Interaction between intracellular vacuoles and the cell surface analysed by finite aperture theory interference reflection microscopy.

abstract：:During early stages of meiotic prophase I the nucleus undergoes considerable reorganization, including the clustering of telomeres, the release of contacts between chromosomes and the nuclear membrane, the reorganization of the nucleolus, and chromatin remodeling. Using a light squashing technique for the analysis of ...

journal_title：Journal of cell science

authors： Yang X,Timofejeva L,Ma H,Makaroff CA

更新日期：2006-09-15 00:00:00

abstract：:The Schizosaccaromyces pombe protein Flp1p belongs to a conserved family of serine-threonine-phosphatases. The founding member of this family, Saccharomyces cerevisiae Cdc14p, is required for inactivation of mitotic CDKs and reversal of CDK mediated phosphorylation at the end of mitosis, thereby bringing about the M-G...

journal_title：Journal of cell science

authors： Esteban V,Blanco M,Cueille N,Simanis V,Moreno S,Bueno A

更新日期：2004-05-15 00:00:00

abstract：:A conjugate of horseradish peroxidase (HRP) to poly(L-lysine) (PLL) was used as a non-specific adsorptive probe to study transcytosis in MDCK strain I and Caco-2 epithelial cells. As we have shown previously, HRP-PLL transcytosis proceeds via an intracellular, non-lysosomal proteolytic compartment in MDCK cells; yet, ...

journal_title：Journal of cell science

authors： Taub ME,Shen WC

更新日期：1993-12-01 00:00:00

abstract：:U937 human promonocytic leukemia cells express PKC isozymes beta 1, beta 2, epsilon and zeta. Indirect immunocytofluorescence using affinity-purified PKC-specific antibodies indicates that each of the endogenous PKC isozymes in U937 cells display a unique compartmentalization within the intact cell. PKC-beta 1 is dist...

journal_title：Journal of cell science

authors： Kiley SC,Parker PJ

更新日期：1995-03-01 00:00:00

abstract：:BMP-dependent patterning in the Drosophila melanogaster wing imaginal disc serves as a paradigm to understand how morphogens specify cell fates. The observed profile of the transcriptional response to the graded signal of BMP relies upon two counter-active gradients of pMad and Brinker (Brk). This patterning model is ...

journal_title：Journal of cell science

authors： Ziv O,Finkelstein R,Suissa Y,Dinur T,Deshpande G,Gerlitz O

更新日期：2012-12-01 00:00:00

abstract：:To examine the role of the keratin head region and its subdomains in filament assembly we constructed several deletion mutants of type I and type II keratins and analysed their in vitro IF forming capacity. The delta K8 (1-74) and delta K18 (1-56), mutants formed only soluble oligomers, predominantly tetramers with th...

journal_title：Journal of cell science

authors： Hatzfeld M,Burba M

更新日期：1994-07-01 00:00:00

abstract：:The repair of injured tissue must occur rapidly to prevent microbial invasion and maintain tissue integrity. Epithelial tissues in particular, which serve as a barrier against the external environment, must repair efficiently in order to restore their primary function. Here we analyze the effect of different parameter...

journal_title：Journal of cell science

authors： Abreu-Blanco MT,Verboon JM,Liu R,Watts JJ,Parkhurst SM

更新日期：2012-12-15 00:00:00

abstract：:During the assembly of Plasmodium falciparum merozoites within the schizont stage, the parasite synthesizes and positions three sets of secretory vesicles (rhoptries, micronemes and dense granules) that are active during red cell invasion. There are up to 40 micronemes per merozoite, shaped like long-necked bottles, a...

journal_title：Journal of cell science

authors： Bannister LH,Hopkins JM,Dluzewski AR,Margos G,Williams IT,Blackman MJ,Kocken CH,Thomas AW,Mitchell GH

更新日期：2003-09-15 00:00:00

abstract：:In our previous experiments with linear strips of adhesive substrate, we found that elongated cultured fibroblasts preserve their length regardless of cell width and the number of cytoplasmic processes. This constancy of length was called 'length control'. In contrast to fibroblasts, single cultured epitheliocytes hav...

journal_title：Journal of cell science

authors： Kharitonova MA,Vasiliev JM

更新日期：2004-04-15 00:00:00

abstract：:Promyelocytic leukemia nuclear bodies (PML-NBs) are mobile subnuclear organelles formed by PML and Sp100 protein. They have been reported to have a role in transcription, DNA replication and repair, telomere lengthening, cell cycle control and tumor suppression. We have conducted high-resolution 4Pi fluorescence laser...

journal_title：Journal of cell science

authors： Lang M,Jegou T,Chung I,Richter K,Münch S,Udvarhelyi A,Cremer C,Hemmerich P,Engelhardt J,Hell SW,Rippe K

更新日期：2010-02-01 00:00:00

abstract：:An electron-microscopic study of various fractions taken during a newly developed method for preparation of brush border vesicles from rat duodenum, has shown that vesiculation of microvilli can occur in situ on the brush border membrane. This mode of formation ensures that the vesicle membranes are oriented as in viv...

journal_title：Journal of cell science

authors： Hearn PR,Russell RG,Farmer J

更新日期：1981-02-01 00:00:00

abstract：:Schistosomula of Schistosoma mansoni bind human low density lipoproteins (LDL) in a concentration-dependent and saturable manner. The bound LDL could provide phospholipids and sterol to the worm, which cannot synthesize sterol de novo and lacks acyl chain-modifying capability. Here we have used three phospholipid anal...

journal_title：Journal of cell science

authors： Caulfield JP,Chiang CP,Yacono PW,Smith LA,Golan DE

更新日期：1991-05-01 00:00:00

abstract：:Voltage-gated Ca2+ (CaV) channels couple membrane depolarization to Ca2+ influx, triggering a range of Ca2+-dependent cellular processes. CaV channels are, therefore, crucial in shaping neuronal activity and function, depending on their individual temporal and spatial properties. Furthermore, many neurotransmitters an...

journal_title：Journal of cell science

authors： Mustafá ER,López Soto EJ,Martínez Damonte V,Rodríguez SS,Lipscombe D,Raingo J

更新日期：2017-11-15 00:00:00

abstract：:Axonemal precursor tubulin is the major protein component of the detergent-soluble membrane/matrix fraction of sea urchin embryonic cilia. Its unusual abundance may reflect the rapid turnover of these cilia, a process that is further documented here. However, whether during induced regeneration or normal turnover and ...

journal_title：Journal of cell science

authors： Stephens RE

更新日期：1994-02-01 00:00:00

abstract：:Dystrophin has been proposed to associate with the skeletal muscle membrane by way of a glycoprotein complex that interacts with its C-terminal domains. Transfection of mdx mouse myotubes in culture or myofibres in vivo with recombinant genes encoding human dystrophin deletion mutants shows, however, that not only the...

journal_title：Journal of cell science

authors： Dunckley MG,Wells KE,Piper TA,Wells DJ,Dickson G

更新日期：1994-06-01 00:00:00

abstract：:The formation of cytoplasmic stress granules and the innate immune response are two distinct cellular stress responses. Our study investigated the involvement of four innate immune proteins - retinoic-acid-inducible gene I (RIG-I, also known as DDX58), melanoma differentiation-associated gene 5 (MDA5, also known as IF...

journal_title：Journal of cell science

authors： Zhang P,Li Y,Xia J,He J,Pu J,Xie J,Wu S,Feng L,Huang X,Zhang P

更新日期：2014-06-01 00:00:00

abstract：:Despite observations that the lacrimal gland has been identified as the principal source of dimeric immunoglobulin A (dIgA) in tears, the mechanism used by lacrimal gland acinar cells (LGACs) to transcytose dIgA produced by interstitial plasma cells is not well-characterized. This study identifies a transcytotic pathw...

journal_title：Journal of cell science

authors： Xu S,Edman M,Kothawala MS,Sun G,Chiang L,Mircheff A,Zhu L,Okamoto C,Hamm-Alvarez S

更新日期：2011-10-15 00:00:00

abstract：:Inositol-(1,4,5)-triphosphate receptors (InsP(3)Rs) are ligand-gated Ca(2+) channels that control Ca(2+) release from intracellular stores and play a central role in a wide range of cellular responses. In most epithelial cells, InsP(3)Rs are not uniformly distributed within the endoplasmic reticulum (ER) membrane, wit...

journal_title：Journal of cell science

authors： Dingli F,Parys JB,Loew D,Saule S,Mery L

更新日期：2012-11-15 00:00:00

abstract：:Enterotoxigenic Bacteroides fragilis - organisms that live in the colon - secrete a metalloprotease toxin, B. fragilis toxin. This toxin binds to a specific intestinal epithelial cell receptor and stimulates cell proliferation, which is dependent, in part, on E-cadherin degradation and beta-catenin-T-cell-factor nucle...

journal_title：Journal of cell science

authors： Wu S,Rhee KJ,Zhang M,Franco A,Sears CL

更新日期：2007-06-01 00:00:00

abstract：:Cell migration and invasion play a crucial role during normal and pathological development. The expression of several members of the Ets family of transcription factors has been shown to correlate with the occurrence of these processes. In the present study, we investigated the effect of the expression of Ets1-DB, the...

journal_title：Journal of cell science

authors： Delannoy-Courdent A,Mattot V,Fafeur V,Fauquette W,Pollet I,Calmels T,Vercamer C,Boilly B,Vandenbunder B,Desbiens X

更新日期：1998-06-01 00:00:00

abstract：:Iron (Fe(2+), Fe(3+)) homeostasis is a tightly regulated process, involving precise control of iron influx and egress from cells. Although the mechanisms of its import into cells by iron carrier molecules are well characterized, iron export remains poorly understood. The current paradigm envisages unique functions ass...

journal_title：Journal of cell science

authors： Sheokand N,Malhotra H,Kumar S,Tillu VA,Chauhan AS,Raje CI,Raje M

更新日期：2014-10-01 00:00:00

abstract：:Phosphatidylinositol-3-phosphate [PtdIns(3)P] regulates endocytic and autophagic membrane traffic. In order to understand the downstream effects of PtdIns(3)P in these processes, it is important to identify PtdIns(3)P-binding proteins, many of which contain FYVE zinc-finger domains. Here, we describe a novel giant FYV...

journal_title：Journal of cell science

authors： Simonsen A,Birkeland HC,Gillooly DJ,Mizushima N,Kuma A,Yoshimori T,Slagsvold T,Brech A,Stenmark H

更新日期：2004-08-15 00:00:00

abstract：:Moving the nucleus to a specific position within the cell is an important event during many cell and developmental processes. Several different molecular mechanisms exist to position nuclei in various cell types. In this Commentary, we review the recent progress made in elucidating mechanisms of nuclear migration in a...

journal_title：Journal of cell science

authors： Bone CR,Starr DA

更新日期：2016-05-15 00:00:00

abstract：:It has long been established that neuronal growth cone navigation depends on changes in microtubule (MT) and F-actin architecture downstream of guidance cues. However, the mechanisms by which MTs and F-actin are dually coordinated remain a fundamentally unresolved question. Here, we report that the well-characterized ...

journal_title：Journal of cell science

authors： Slater PG,Cammarata GM,Samuelson AG,Magee A,Hu Y,Lowery LA

更新日期：2019-04-30 00:00:00

abstract：:Prox1 is a master regulator for the development of lymphatic vasculature and the induction of lymphangiogenesis. In this study, we identified Prox1 as a new target for small ubiquitin-like modifier 1 (SUMO-1). Lysine 556 (K556) was found to be the major sumoylation site for Prox1 in vitro and in vivo. Mutation of this...

journal_title：Journal of cell science

authors： Pan MR,Chang TM,Chang HC,Su JL,Wang HW,Hung WC

更新日期：2009-09-15 00:00:00

abstract：:The intracellular protozoan parasite Toxoplasma gondii, like all members of the phylum Apicomplexa, is known to possess many organelles: in addition to mitochondria and the compartments of the secretory pathway, there is a reduced chloroplast (the apicoplast) and the phylum-specific components of the apical complex: d...

journal_title：Journal of cell science

authors： Ding M,Clayton C,Soldati D

更新日期：2000-07-01 00:00:00

abstract：:The Arabidopsis thaliana ASY1 gene is essential for homologous chromosome synapsis. Antibodies specific to Asy1 protein and its homologue BoAsy1 from the related crop species Brassica oleracea have been used to investigate the temporal expression and localization of the protein in both species. Asy1 is initially detec...

journal_title：Journal of cell science

authors： Armstrong SJ,Caryl AP,Jones GH,Franklin FC

更新日期：2002-09-15 00:00:00

abstract：:Recent studies show that mitochondria and actin filaments work together in two contexts: (1) increased cytoplasmic calcium induces cytoplasmic actin polymerization that stimulates mitochondrial fission and (2) mitochondrial depolarization causes actin assembly around mitochondria, with roles in mitophagy. It is unclea...

journal_title：Journal of cell science

authors： Fung TS,Ji WK,Higgs HN,Chakrabarti R

更新日期：2019-09-23 00:00:00

abstract：:The Dlg tumour suppressor protein is intimately involved in the control of cell contact and polarity. Previous studies have shown that hDlg is a target for a number of viral transforming proteins. In particular, the high risk human papillomavirus (HPV) E6 proteins target hDlg for proteasome-mediated degradation, an ac...

journal_title：Journal of cell science

authors： Mantovani F,Massimi P,Banks L

更新日期：2001-12-01 00:00:00

abstract：:Cells experience mechanical stresses in different physiological and pathological settings. Clathrin-coated structures (CCSs) are sensitive to such perturbations in a way that often results in a mechanical impairment of endocytic budding. Compressive stress is a mechanical perturbation that leads to increased membrane ...

journal_title：Journal of cell science

authors： Baschieri F,Le Devedec D,Tettarasar S,Elkhatib N,Montagnac G

更新日期：2020-09-01 00:00:00