Abstract：
:The constitutive (or default) pathway for protein secretion was investigated in two epithelial cells, Madin-Darby canine kidney (MDCK) and human colonic adenocarcinoma (Caco-2), using a bacterial enzyme. The choice of a bacterial protein was based on the requirement to identify a protein devoid of sorting signals. The sorting of a bacterial endoglucanase derived from Clostridium thermocellum, endoglucanase E, from stably transfected MDCK and Caco-2 cells was examined. The choice of a bacterial endoglucanase for these studies has advantages of simple, sensitive and quantitative detection, while higher eukaryotic cells do not express endoglucanase activity. Both cell lines secreted a 50 kDa form of the bacterial protein, while smaller intracellular forms were also observed. In polarized layers of MDCK cells the endoglucanase was secreted into both membrane domains in the ratio 62% apical and 38% basolateral. In Caco-2 cells secretion was predominantly, 70%, through the basolateral membrane. These results define the constitutive pathway for protein secretion in these two model epithelial cells.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Soole KL,Hall J,Jepson MA,Hazlewood GP,Gilbert HJ,Hirst BHkeywords:
subject
Has Abstractpub_date
1992-07-01 00:00:00pages
495-504eissn
0021-9533issn
1477-9137journal_volume
102 ( Pt 3)pub_type
杂志文章abstract：:We have used genetic and molecular techniques to investigate the interactions among genes required for the initiation and regulation of septum formation in Schizosaccharomyces pombe. Our data suggest that the products of the cdc7, cdc11, cdc14 and cdc16 genes interact. These activities may regulate the function of the...
journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：1992-04-01 00:00:00
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journal_title：Journal of cell science
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pub_type： 杂志文章
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更新日期：2018-07-19 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03030
更新日期：2006-07-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：1979-10-01 00:00:00
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journal_title：Journal of cell science
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更新日期：1979-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：1978-12-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01573
更新日期：2004-12-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2016-10-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1980-08-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.129031
更新日期：2014-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1242/jcs.246934
更新日期：2020-08-21 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2018-11-16 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.023143
更新日期：2008-01-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03217
更新日期：2006-10-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03020
更新日期：2006-07-01 00:00:00
abstract：:Oncogenic forms of the non-receptor tyrosine kinase Src alter cell structure, in particular the actin cytoskeleton and the adhesion networks that control cell migration, and also transmit signals that regulate proliferation and cell survival. Recent work indicates that they do so by influencing the RhoA-ROCK pathway t...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.01111
更新日期：2004-03-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2015-12-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01168
更新日期：2004-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章,评审
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更新日期：2020-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.247700
更新日期：2020-10-23 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.022103
更新日期：2008-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1988-03-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03080
更新日期：2006-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.231357
更新日期：2019-10-09 00:00:00
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pub_type： 杂志文章
doi：10.1242/jcs.205625
更新日期：2018-01-04 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-05-01 00:00:00
