Abstract：
:Cytokinesis in many eukaryotes requires a contractile actomyosin ring that is placed at the division site. In fission yeast, which is an attractive organism for the study of cytokinesis, actomyosin ring assembly and contraction requires the myosin II heavy chain Myo2p. Although myo2-E1, a temperature-sensitive mutant defective in the upper 50 kDa domain of Myo2p, has been studied extensively, the molecular basis of the cytokinesis defect is not understood. Here, we isolate myo2-E1-Sup2, an intragenic suppressor that contains the original mutation in myo2-E1 (G345R) and a second mutation in the upper 50 kDa domain (Y297C). Unlike myo2-E1-Sup1, a previously characterized myo2-E1 suppressor, myo2-E1-Sup2 reverses actomyosin ring contraction defects in vitro and in vivo Structural analysis of available myosin motor domain conformations suggests that a steric clash in myo2-E1, which is caused by the replacement of a glycine with a bulky arginine, is relieved in myo2-E1-Sup2 by mutation of a tyrosine to a smaller cysteine. Our work provides insight into the function of the upper 50 kDa domain of Myo2p, informs a molecular basis for the cytokinesis defect in myo2-E1, and may be relevant to the understanding of certain cardiomyopathies.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Palani S,Srinivasan R,Zambon P,Kamnev A,Gayathri P,Balasubramanian MKdoi
10.1242/jcs.205625subject
Has Abstractpub_date
2018-01-04 00:00:00issue
1eissn
0021-9533issn
1477-9137pii
jcs.205625journal_volume
131pub_type
杂志文章abstract：:The cell nucleus is highly organized. Many nuclear functions are localized in discrete domains, suggesting that compartmentalization is an important aspect of the regulation and coordination of nuclear functions. We investigated the subnuclear distribution of the glucocorticoid receptor, a hormone-dependent transcript...
journal_title：Journal of cell science
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journal_title：Journal of cell science
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更新日期：1983-09-01 00:00:00
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journal_title：Journal of cell science
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更新日期：1979-10-01 00:00:00
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journal_title：Journal of cell science
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更新日期：1995-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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