Abstract:
:We have previously shown by examining the anchorage dependence, density-dependent inhibition of growth, LETS protein and microfilament bundles that the transformed phenotype of the parental tumours are suppressed in hybrids between rat embryo fibroblast (REF) and mouse tumour cells (TA3B). Hybrids between TA3B and Syrian hamster sarcoma cells (BHK-B1) also show suppression. We now demonstrate that tumorigenicity in nude mice is also suppressed in TA3B X REF and B1 X TA3B hybrids. Tumours arise from the suppressed hybrids by the selective outgrowth of variants with properties different from the majority of cells inoculated. These tumour variants always had an altered cytoskeletin but 1 out of 14 cases retained anchorage-dependent growth. Selection in culture for anchorage-independent growth selected for tumorigenicity. The coordinate suppression of the transformed phenotype and tumorigenicity could be explained by postulating a pleiotropic control mechanism affecting both. However, the occasional dissociation of tumorigenicity from anchorage dependence in the variants suggests that the targets for the control mechanism must be different.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Marshall CJsubject
Has Abstractpub_date
1979-10-01 00:00:00pages
319-27eissn
0021-9533issn
1477-9137journal_volume
39pub_type
杂志文章abstract::AMP-activated protein kinase (AMPK), a cellular metabolic sensor, is essential in energy regulation and metabolism. Hepatocyte polarization during liver development and regeneration parallels increased metabolism. The current study investigates the effects of AMPK and its upstream activator LKB1 on polarity and bile c...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.068098
更新日期:2010-10-01 00:00:00
abstract::In this paper we examine DNA repair in the mouse. Using stringently applied inhibitors of DNA synthesis (hydroxyurea and 1-beta-D-arabinofuranosylcytosine) after ultraviolet irradiation we have quantitated an early step in the repair process - the frequency of single-strand DNA breaks, products of enzymic incision--in...
journal_title:Journal of cell science
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更新日期:1983-03-01 00:00:00
abstract::By operating as both a subunit of the cadherin complex and a key component of Wnt signalling, β-catenin acts as the lynchpin between cell-cell contact and transcriptional regulation of proliferation, coordinating epithelial tissue homeostasis and regeneration. The integration of multiple growth-regulatory inputs with ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.150888
更新日期:2014-07-01 00:00:00
abstract::Cornichon proteins are structurally related transmembrane proteins that have been studied in and Drosophila and yeast. In Drosophila, Cornichon (Cni) is involved in embryo polarization by the TGFalpha-related Gurken. In yeast, the Cni-related Erv14 is required for axial budding. A cargo receptor function has been prop...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.004200
更新日期:2007-07-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1993-09-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-05-15 00:00:00
abstract::A myosin heavy chain polypeptide has been identified and localized in Nicotiana pollen tubes using monoclonal anti-myosin antibodies. The epitopes of these antibodies were found to reside on the myosin heavy chain head and rod portion and were, therefore, designated anti-S-1 (myosin S-1) and anti-LMM (light meromyosin...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1989-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.060319
更新日期:2010-03-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00403
更新日期:2003-05-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.132613
更新日期:2013-12-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
abstract::Blocking expression of EhCaBP1, a calmodulin-like, four EF-hand protein from the protozoan parasite Entamoeba histolytica, resulted in inhibition of cellular proliferation. In this paper we report that EhCaBP1 is involved in dynamic changes of the actin cytoskeleton. Both endocytosis and phagocytosis were severely imp...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01198
更新日期:2004-07-15 00:00:00
abstract::The surface coat of procyclic forms of Trypanosoma brucei consists of related, internally repetitive glycoproteins known as EP and GPEET procyclins. Previously we showed that the extracellular domain of GPEET is phosphorylated. We now show that phosphorylation of this glycosylphosphatidylinositol-anchored surface prot...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract::Distinctive views of the tubulo-vesicular elements interposed between the endoplasmic reticulum (ER) and the Golgi apparatus were obtained in thin sections. The tubules that protrude from the transitional rough ER (tRER) are of dissimilar length. The numbers of tubules and of the nearby omega- and pear-shaped profiles...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-03-01 00:00:00
abstract::Primary cilia are conserved organelles that play crucial roles as mechano- and chemosensors, as well as transducing signaling cascades. Consequently, ciliary dysfunction results in a broad range of phenotypes: the ciliopathies. Bardet-Biedl syndrome (BBS), a model ciliopathy, is caused by mutations in 16 known genes. ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.089375
更新日期:2012-01-15 00:00:00
abstract::Phosphoinositides regulate numerous processes in various subcellular compartments. Whereas many stimuli trigger changes in the plasma-membrane PtdIns(4,5)P(2) concentration, little is known about its precursor, PtdIns(4)P, in particular whether there are stimulus-induced alterations independent of those of PtdIns(4,5)...
journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2010-05-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.100149
更新日期:2012-03-15 00:00:00
abstract::Expression of a dominant negative fragment of the spectrin family member Syne-1 causes an accumulation of binucleate cells, suggesting a role for this protein in cytokinesis. An association of this fragment with the C-terminal tail domain of the kinesin II subunit KIF3B was identified by yeast two-hybrid and co-precip...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00892
更新日期:2004-02-01 00:00:00
abstract::An electron microscopic investigation of the events associated with meiosis in Lilium has revealed a number of changes in both the organellar population and the other cytoplasmic components. Ribosome numbers decrease significantly in early prophase and are later replenished in the tetrads, a process most likely involv...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::During cell division, misaligned chromosomes are captured and aligned by motors before their segregation. The CENP-E motor is recruited to polar unattached kinetochores to facilitate chromosome alignment. The spindle checkpoint protein BubR1 (also known as BUB1B) has been reported as a CENP-E interacting partner, but ...
journal_title:Journal of cell science
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更新日期:2020-08-25 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.146316
更新日期:2014-05-15 00:00:00
abstract::Aminopeptidase N purified from whole Manduca sexta midgut binds the Cry1Ac insecticidal toxin from Bacillus thuringiensis and this binding is inhibited by N-acetylgalactosamine (GalNAc). We have examined the membrane permeabilising activity of the Cry1Ac toxin using brush border membrane vesicles (BBMV) prepared from ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1997-12-01 00:00:00
abstract::Transcription factor E2F plays an important role in coordinating and integrating early cell cycle progression with the transcription apparatus. It is known that physiological E2F arises when a member of two families of proteins, E2F and DP, interact as E2F/DP heterodimers and that transcriptional activity is regulated...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00
abstract::The integrin alphavbeta6 is expressed on a variety of epithelial cells during dynamic processes including organogenesis, tissue injury and malignant transformation. However, because of the lack of tools to specifically inhibit the function of this integrin, little is known about its effects on cell behavior. To direct...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
abstract::Paramecium tetraurelia is a unicellular organism that utilizes both axonemal and cytoplasmic dyneins. The highly conserved region containing the catalytic P-loop of the dynein heavy chain was amplified by RNA-directed polymerase chain reaction. Eight different P-loop-containing cDNA fragments were cloned. Southern hyb...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract::Podosomes are highly dynamic actin-based structures commonly found in motile and invasive cells such as macrophages, osteoclasts and vascular smooth muscle cells. Here, we have investigated the role of caldesmon, an actin-binding protein, in the formation of podosomes in aortic smooth muscle A7r5 cells induced by the ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02881
更新日期:2006-05-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.131045
更新日期:2014-04-15 00:00:00