DNA polymerase alpha, a component of the replication initiation complex, is essential for the checkpoint coupling S phase to mitosis in fission yeast.

abstract：:Endothelial integrity relies on a mechanical crosstalk between intercellular and cell-matrix interactions. This crosstalk is compromised in hemorrhagic vascular lesions of patients carrying loss-of-function mutations in cerebral cavernous malformation (CCM) genes. RhoA/ROCK-dependent cytoskeletal remodeling is central...

journal_title：Journal of cell science

authors： Lisowska J,Rödel CJ,Manet S,Miroshnikova YA,Boyault C,Planus E,De Mets R,Lee HH,Destaing O,Mertani H,Boulday G,Tournier-Lasserve E,Balland M,Abdelilah-Seyfried S,Albiges-Rizo C,Faurobert E

更新日期：2018-08-13 00:00:00

abstract：:The essential function of the T cell receptor (TCR) is to translate the engagement of peptides on the major histocompatibility complex (pMHC) into appropriate intracellular signals through the associated cluster of differentiation 3 (CD3) complex. The spatial organization of the TCR-CD3 complex in the membrane is thou...

journal_title：Journal of cell science

authors： Goyette J,Nieves DJ,Ma Y,Gaus K

更新日期：2019-02-11 00:00:00

abstract：:The constitutive recirculation of lymphocytes between the widely distributed organs of the immune system is essential for host defence. We have developed an in vitro model of lymphocyte migration from the blood into lymph nodes which employs primary cultures of high endothelial cells (HEC). HEC-adherent lymphocytes ad...

journal_title：Journal of cell science

authors： Hourihan H,Allen TD,Ager A

更新日期：1993-04-01 00:00:00

abstract：:Nearly all intermediate filament (IF) proteins share two sequence motifs located at the N- and the C-terminal ends of their helical rod domain ('coil 1a' and 'coil 2b', respectively). To examine the structural role of the coil 2b motif, we have performed in vitro assembly studies and in vivo microinjection experiments...

journal_title：Journal of cell science

authors： Kouklis PD,Traub P,Georgatos SD

更新日期：1992-05-01 00:00:00

abstract：:We have found that Ehrlich ascites tumour (EAT) cells, deprived of any carbon source, and suspended at a density of 2 X 10(5) cells/cm3, begin to die only after 12 h of starvation, though it is known that under these conditions they lose over 80% of their ATP within 30 min. Moreover, we have found that the viability o...

journal_title：Journal of cell science

authors： Zaporowska-Siwiak E,Michalik M,Kajstura J,Korohoda W

更新日期：1985-08-01 00:00:00

abstract：:We have applied fluorescence ratio imaging to the analysis of an actin-binding protein concentration relative to F-actin in macrophages, in order to explore the role of a novel (alpha)-actinin isoform, actinin-4, relative to that of the classical isoform, actinin-1. Conventional immunofluorescence images showed that b...

journal_title：Journal of cell science

authors： Araki N,Hatae T,Yamada T,Hirohashi S

更新日期：2000-09-01 00:00:00

abstract：:Elementary Ca(2+) signals, such as "Ca(2+) puffs", which arise from the activation of inositol 1,4,5-trisphosphate receptors, are building blocks for local and global Ca(2+) signalling. We characterized Ca(2+) puffs in six cell types that expressed differing ratios of the three inositol 1,4,5-trisphosphate receptor is...

journal_title：Journal of cell science

authors： Tovey SC,de Smet P,Lipp P,Thomas D,Young KW,Missiaen L,De Smedt H,Parys JB,Berridge MJ,Thuring J,Holmes A,Bootman MD

更新日期：2001-11-01 00:00:00

abstract：:Autophagy is fundamental for cell and organismal health. Two types of autophagy are conserved in eukaryotes: macroautophagy and microautophagy. During macroautophagy, autophagosomes deliver cytoplasmic constituents to endosomes or lysosomes, whereas during microautophagy lytic organelles take up cytoplasm directly. Wh...

journal_title：Journal of cell science

authors： Schuck S

更新日期：2020-09-09 00:00:00

abstract：:Class-V myosins are two-headed actin-based mechanoenzymes that function in the transport and subcellular localization of organelles and possibly in the outgrowth of cellular processes. To determine which domains of myosin-V are involved in intracellular localization of this motor protein, we have expressed fusions of ...

journal_title：Journal of cell science

authors： Tsakraklides V,Krogh K,Wang L,Bizario JC,Larson RE,Espreafico EM,Wolenski JS

更新日期：1999-09-01 00:00:00

abstract：:Barrier junctions prevent pathogen invasion and restrict paracellular leakage across epithelial sheets. To understand how one barrier junction, the septate junction (SJ), is regulated in vivo, we used fluorescence recovery after photobleaching (FRAP) to examine SJ protein dynamics in Drosophila. Most SJ-associated pro...

journal_title：Journal of cell science

authors： Oshima K,Fehon RG

更新日期：2011-08-15 00:00:00

abstract：:Several studies have shown that altered gravity conditions influence mammalian cell growth and differentiation. The molecular mechanisms underlying these effects, however, remain relatively obscure. In this paper we show that microgravity reached in a sounding rocket strongly decreases epidermal growth factor (EGF)-in...

journal_title：Journal of cell science

authors： de Groot RP,Rijken PJ,den Hertog J,Boonstra J,Verkleij AJ,de Laat SW,Kruijer W

更新日期：1990-09-01 00:00:00

abstract：:Current methodologies for targeting the mitochondrial genome for research and/or therapy development in mitochondrial diseases are restricted by practical limitations and technical inflexibility. A molecular toolbox for CRISPR-mediated mitochondrial genome editing is desirable, as this could enable targeting of mtDNA ...

journal_title：Journal of cell science

authors： Antón Z,Mullally G,Ford HC,van der Kamp MW,Szczelkun MD,Lane JD

更新日期：2020-09-16 00:00:00

abstract：:Embryonic stem (ES) cells are cells derived from the early embryo that can be propagated indefinitely in the primitive undifferentiated state while remaining pluripotent; they share these properties with embryonic germ (EG) cells. Candidate ES and EG cell lines from the human blastocyst and embryonic gonad can differe...

journal_title：Journal of cell science

authors： Pera MF,Reubinoff B,Trounson A

更新日期：2000-01-01 00:00:00

abstract：:Human cytomegalovirus (HCMV) establishes life-long latent infection in hematopoietic progenitor cells and circulating monocytes in infected individuals. Myeloid differentiation coupled with immune dysregulation leads to viral reactivation, which can cause severe disease and mortality. Reactivation of latent virus requ...

journal_title：Journal of cell science

authors： Krishna BA,Wass AB,Dooley AL,O'Connor CM

更新日期：2021-01-25 00:00:00

abstract：:Wound healing crucially relies on the mechanical activity of fibroblasts responding to TGFβ1 and to forces transmitted across focal adhesions. Integrin-linked kinase (ILK) is a central adapter recruited to integrin β1 tails in focal adhesions mediating the communication between cells and extracellular matrix. Here, we...

journal_title：Journal of cell science

authors： Blumbach K,Zweers MC,Brunner G,Peters AS,Schmitz M,Schulz JN,Schild A,Denton CP,Sakai T,Fässler R,Krieg T,Eckes B

更新日期：2010-11-15 00:00:00

abstract：:Dystrophin has been proposed to associate with the skeletal muscle membrane by way of a glycoprotein complex that interacts with its C-terminal domains. Transfection of mdx mouse myotubes in culture or myofibres in vivo with recombinant genes encoding human dystrophin deletion mutants shows, however, that not only the...

journal_title：Journal of cell science

authors： Dunckley MG,Wells KE,Piper TA,Wells DJ,Dickson G

更新日期：1994-06-01 00:00:00

abstract：:Vimentin expression in human mammary epithelial MCF10A cells was examined as a function of their migratory status using an in vitro wound-healing model. Analysis of the trajectories of the cells and their migratory speeds by time lapse-video microscopy revealed that vimentin mRNA and protein expression were exclusivel...

journal_title：Journal of cell science

authors： Gilles C,Polette M,Zahm JM,Tournier JM,Volders L,Foidart JM,Birembaut P

更新日期：1999-12-01 00:00:00

abstract：:Niemann-Pick disease type C (NPC) is an inherited lipid storage disorder caused by mutations in NPC1 or NPC2. NPC1 is a polytopic glycoprotein that contains a sterol-sensing domain, whereas NPC2 is a soluble protein that contains an MD-2-like lipid-recognition domain. In the current study, we addressed the hypothesis ...

journal_title：Journal of cell science

authors： Ohsaki Y,Sugimoto Y,Suzuki M,Hosokawa H,Yoshimori T,Davies JP,Ioannou YA,Vanier MT,Ohno K,Ninomiya H

更新日期：2006-07-01 00:00:00

abstract：:Microscopic green algae inhabiting desert microbiotic crusts are remarkably diverse phylogenetically, and many desert lineages have independently evolved from aquatic ancestors. Here we worked with five desert and aquatic species within the family Scenedesmaceae to examine mechanisms that underlie desiccation toleranc...

journal_title：Journal of cell science

authors： Cardon ZG,Peredo EL,Dohnalkova AC,Gershone HL,Bezanilla M

更新日期：2018-04-10 00:00:00

abstract：:We investigated the in vivo localisation of fission yeast cyclin-dependent kinase cdc2p during mitosis and meiosis. Fusion to yellow fluorescent protein (YFP) revealed that cdc2-YFP is present in the cytoplasm at all stages of the cell cycle. Nuclear cdc2-YFP fluorescence oscillates with that of cdc13-YFP cyclin. At G...

journal_title：Journal of cell science

authors： Decottignies A,Zarzov P,Nurse P

更新日期：2001-07-01 00:00:00

abstract：:During their development, mammalian oocytes acquire the ability to resume meiosis. We demonstrate that the concentration of p34cdc2 increases during the acquisition of meiotic competence, as determined by immunoblotting, whereas the concentration of cyclin B1 decreases. Laser-scanning confocal microscopy corroborated ...

journal_title：Journal of cell science

authors： Mitra J,Schultz RM

更新日期：1996-09-01 00:00:00

abstract：:We have demonstrated the possibility of cross-talk between E-cadherin and alphav integrins in breast carcinoma cells. Using the function-blocking anti-alphav monoclonal antibody 17E6, applied to monolayer cultures of breast cancer lines, it was found that treatment of cells possessing detergent-insoluble (implying att...

journal_title：Journal of cell science

authors： von Schlippe M,Marshall JF,Perry P,Stone M,Zhu AJ,Hart IR

更新日期：2000-02-01 00:00:00

abstract：:The establishment of a tissue-culture system for epithelial cells derived from human foetal pancreas has recently been reported. Further analyses have now been made on these cells in vitro, together with parallel investigation of the distribution of different cell types within the intact foetal pancreas. Results suppo...

journal_title：Journal of cell science

authors： Harris A,Coleman L

更新日期：1988-05-01 00:00:00

abstract：:The extracellular matrix (ECM) regulates cell behavior by influencing cell proliferation, survival, shape, migration and differentiation. Far from being a static structure, the ECM is constantly undergoing remodeling--i.e. assembly and degradation--particularly during the normal processes of development, differentiati...

journal_title：Journal of cell science

authors： Daley WP,Peters SB,Larsen M

更新日期：2008-02-01 00:00:00

abstract：:We investigated mechanisms of the p73alpha-mediated repression of the platelet-derived growth factor beta-receptor (PDGFRB) promoter caused by its interaction with NF-Y. Treatment of cells with the histone deacetylase (HDAC) inhibitor, Trichostatin A, increases PDGFRB promoter activity through the CCAAT motif and coun...

journal_title：Journal of cell science

authors： Uramoto H,Wetterskog D,Hackzell A,Matsumoto Y,Funa K

更新日期：2004-10-15 00:00:00

abstract：:The cell surface delivery of extracellular matrix (ECM) and integrins is fundamental for cell migration in wound healing and during cancer cell metastasis. This process is not only driven by several soluble NSF attachment protein (SNAP) receptor (SNARE) proteins, which are key players in vesicle transport at the cell ...

journal_title：Journal of cell science

authors： Enrich C,Rentero C,Hierro A,Grewal T

更新日期：2015-03-15 00:00:00

abstract：:The distribution of anionic sites on the membranes of rat pancreatic B cells and of their storage granules has been studied by the use of a visual probe of cationic ferritin. Membranes of isolated storage granules possessed a net negative charge which was apparently evenly distributed; the number of anionic sites was ...

journal_title：Journal of cell science

authors： Howell SL,Tyhurst M

更新日期：1977-01-01 00:00:00

abstract：:We have isolated the human genes encoding the Pyst1 (MKP-3) and Pyst2 (MKP-X) MAP kinase phosphatases. Both genes consist of three exons interrupted by two introns and lack an intron which is conserved in all the other members of this gene family characterised to date. This reinforces the conclusion that Pyst1 and Pys...

journal_title：Journal of cell science

authors： Dowd S,Sneddon AA,Keyse SM

更新日期：1998-11-01 00:00:00

abstract：:Ca2+ is a second messenger in many physiological and phytopathological processes. Peroxisomes are subcellular compartments with an active oxidative and nitrosative metabolism. Previous studies have demonstrated that peroxisomal nitric oxide (NO) generation is dependent on Ca2+ and calmodulin (CaM). Here, we used Arabi...

journal_title：Journal of cell science

authors： Corpas FJ,Barroso JB

更新日期：2018-01-29 00:00:00

abstract：:The vacuolar H(+)-ATPase (V-ATPase) establishes pH gradients along secretory and endocytic pathways. Progressive acidification is essential for proteolytic processing of prohormones and aggregation of soluble content proteins. The V-ATPase V(0) subunit is thought to have a separate role in budding and fusion events. P...

journal_title：Journal of cell science

authors： Sobota JA,Bäck N,Eipper BA,Mains RE

更新日期：2009-10-01 00:00:00