Optimizing live-cell fluorescence imaging conditions to minimize phototoxicity.

abstract：:The vacuolar H(+)-ATPase (V-ATPase) is a multi-subunit enzyme that plays important roles in eukaryotic cells. In Dictyostelium, it is found primarily in membranes of the contractile vacuole complex, where it energizes fluid accumulation by this osmoregulatory organelle and also in membranes of endolysosomes, where it ...

journal_title：Journal of cell science

authors： Clarke M,Köhler J,Arana Q,Liu T,Heuser J,Gerisch G

更新日期：2002-07-15 00:00:00

abstract：:Human erythrocytes suspended in buffered isotonic saline were frozen to the temperature of liquid nitrogen at various cooling rates of 3, 140, 700, 1800, 3500, 8000 and 11 500 deg. C/min. The membrane ultrastructure in the frozen state and the extent of haemolysis after thawing were examined at each cooling rate. As t...

journal_title：Journal of cell science

authors： Fujikawa S

更新日期：1981-06-01 00:00:00

abstract：:We have previously reported that foetal and adult fibroblasts display distinctive migratory phenotypes when cultured on three-dimensional collagen gels. Both skin and tumour-derived fibroblasts from a significant proportion of patients with breast cancer were subsequently observed to display foetal-like migratory beha...

journal_title：Journal of cell science

authors： Schor SL,Schor AM,Rushton G

更新日期：1988-07-01 00:00:00

abstract：:Rubella virus (RV) envelope glycoproteins E1 and E2 are targeted to the Golgi as heterodimers. While E2 contains a transmembrane Golgi retention signal, E1 is arrested in a pre-Golgi compartment in the absence of E2, and appears to require heterodimerization in order to reach the Golgi. Various forms of E1 with deleti...

journal_title：Journal of cell science

authors： Bernasconi E,Fasel N,Wittek R

更新日期：1996-06-01 00:00:00

abstract：:The existence of two forms of the chromosome passenger complex (CPC) in the mammalian oocyte has meant that its role in female meiosis has remained unclear. Here we use loss- and gain-of function approaches to assess the meiotic functions of one of the shared components of these complexes, INCENP, and of the variable ...

journal_title：Journal of cell science

authors： Sharif B,Na J,Lykke-Hartmann K,McLaughlin SH,Laue E,Glover DM,Zernicka-Goetz M

更新日期：2010-12-15 00:00:00

abstract：:The morphogenesis-related NDR kinase (MOR) pathway regulates morphogenesis in fungi. In spite of the high conservation of its components, impairing their functions results in highly divergent cellular responses depending on the fungal species. The reasons for such differences are unclear. Here we propose that the spec...

journal_title：Journal of cell science

authors： Sartorel E,Pérez-Martín J

更新日期：2012-10-01 00:00:00

abstract：:Hyperthermia adversely affects cell structure and function, but also induces adaptive responses that allow cells to tolerate these stressful conditions. For example, heat-induced expression of the molecular chaperone protein HSP70 can prevent stress-induced cell death by inhibiting signaling pathways that lead to apop...

journal_title：Journal of cell science

authors： Simard JP,Reynolds DN,Kraguljac AP,Smith GS,Mosser DD

更新日期：2011-07-15 00:00:00

abstract：:Inactivation of Simian Virus 40 large T antigen, in cells immortalized with conditional mutants, leads to activation of p53 and apoptosis. We used the mRNA differential display method to identify genes differentially expressed during this process. We found that steady-state levels of mRNA for cytoplasmic actins decrea...

journal_title：Journal of cell science

authors： Guénal I,Risler Y,Mignotte B

更新日期：1997-02-01 00:00:00

abstract：:Concomitant expression of mutant p53 and oncogenic Ras, leading to cellular transformation, is well documented. However, the mechanisms by which the various mutant p53 categories cooperate with Ras remain largely obscure. From this study we suggest that different mutant p53 categories cooperate with H-Ras in different...

journal_title：Journal of cell science

authors： Solomon H,Buganim Y,Kogan-Sakin I,Pomeraniec L,Assia Y,Madar S,Goldstein I,Brosh R,Kalo E,Beatus T,Goldfinger N,Rotter V

更新日期：2012-07-01 00:00:00

abstract：:Rothmund-Thomson syndrome (RTS) is a human genetic disorder characterized by genome instability, cancer susceptibility and premature aging. The gene defective in a subset of RTS cases, RECQL4, encodes a member of the RecQ family of DNA helicases. To better define the function of the RECQL4 protein, we have determined ...

journal_title：Journal of cell science

authors： Petkovic M,Dietschy T,Freire R,Jiao R,Stagljar I

更新日期：2005-09-15 00:00:00

abstract：:In Saccharomyces cerevisiae, DNA replication stress activates the replication checkpoint, which slows S-phase progression, stabilizes slowed or stalled replication forks, and relieves inhibition of the ribonucleotide reductase (RNR) complex. To identify novel genes that promote cellular viability after replication str...

journal_title：Journal of cell science

authors： Woolstencroft RN,Beilharz TH,Cook MA,Preiss T,Durocher D,Tyers M

更新日期：2006-12-15 00:00:00

abstract：:Previous work has shown that phorbol esters or diacylglycerol trigger cortical granule exocytosis in Xenopus oocytes. We sought to identify the isoform(s) of protein kinase C (PKC) that mediate(s) this regulated secretory event. Because this process is initiated by lipid activators of PKC but is independent of calcium...

journal_title：Journal of cell science

authors： Gundersen CB,Kohan SA,Chen Q,Iagnemma J,Umbach JA

更新日期：2002-03-15 00:00:00

abstract：:Although membrane addition is crucial for cytokinesis in many animal cell types, the specific mechanisms supporting cleavage furrow ingression are not yet understood. Mutations in the gene brunelleschi (bru), which encodes the Drosophila ortholog of the yeast Trs120p subunit of TRAPPII, cause failure of furrow ingress...

journal_title：Journal of cell science

authors： Robinett CC,Giansanti MG,Gatti M,Fuller MT

更新日期：2009-12-15 00:00:00

abstract：:The cytostome-cytopharynx complex is the main site for endocytosis in epimastigotes of Trypanosoma cruzi It consists of an opening at the plasma membrane surface - the cytostome - followed by a membrane invagination - the cytopharynx. In G1/S cells, this structure is associated with two specific sets of microtubules, ...

journal_title：Journal of cell science

authors： Alcantara CL,Vidal JC,de Souza W,Cunha-E-Silva NL

更新日期：2017-01-01 00:00:00

abstract：:Many adherent cells in vitro are surrounded by a transparent exclusion zone or halo, several micrometers thick, which red blood cells, bacteria and carbon particles cannot penetrate. This halo is rapidly and specifically removed by hyaluronidase and its high degree of hydration is demonstrated by the fact that, althou...

journal_title：Journal of cell science

authors： Bard JB,McBride WH,Ross AR

更新日期：1983-07-01 00:00:00

abstract：:The ribonucleoprotein enzyme RNase P catalyzes the 5' processing of pre-transfer RNA, and has also recently been implicated in pre-ribosomal RNA processing. In the present investigation, in situ hybridization revealed that RNase P RNA is present throughout the nucleus of mammalian cells. However, rhodamine-labeled hum...

journal_title：Journal of cell science

authors： Jacobson MR,Cao LG,Taneja K,Singer RH,Wang YL,Pederson T

更新日期：1997-04-01 00:00:00

abstract：:The adaptor protein Miranda plays a pivotal role in the asymmetric cell division of neuroblasts by asymmetrically segregating key differentiation factors. Miranda localization requires Myosin VI and Myosin II. The apical-then-basal localization pattern of Miranda detected in fixed tissue, and the localization defects ...

journal_title：Journal of cell science

authors： Erben V,Waldhuber M,Langer D,Fetka I,Jansen RP,Petritsch C

更新日期：2008-05-01 00:00:00

abstract：:The Schizosaccaromyces pombe protein Flp1p belongs to a conserved family of serine-threonine-phosphatases. The founding member of this family, Saccharomyces cerevisiae Cdc14p, is required for inactivation of mitotic CDKs and reversal of CDK mediated phosphorylation at the end of mitosis, thereby bringing about the M-G...

journal_title：Journal of cell science

authors： Esteban V,Blanco M,Cueille N,Simanis V,Moreno S,Bueno A

更新日期：2004-05-15 00:00:00

abstract：:We investigated human Hap50, the large isoform of the previously characterized Hsp70/Hsc70-associating protein Hap46, also called BAG-1, for effects on transcriptional activities. Overproduction by transient transfection led to enhanced expression of reporter gene constructs in various cell types using different promo...

journal_title：Journal of cell science

authors： Niyaz Y,Zeiner M,Gehring U

更新日期：2001-05-01 00:00:00

abstract：:Glomerular mesangial cells (MCs) are central to the pathogenesis of progressive glomeruli-associated renal diseases. However, molecular mechanisms underlying changes in MC functions still remain poorly understood. Here, we show that in MCs, the urokinase-type plasminogen activator (uPA) induces, via its specific recep...

journal_title：Journal of cell science

authors： Shushakova N,Tkachuk N,Dangers M,Tkachuk S,Park JK,Hashimoto K,Haller H,Dumler I

更新日期：2005-06-15 00:00:00

abstract：:Beta-arrestin 1 and beta-arrestin 2 are well-known negative regulators of G-protein-coupled receptor (GPCR) signaling. Upon GPCR activation, beta-arrestins translocate to the cell membrane and bind to the agonist-occupied receptors. This uncouples these receptors from G proteins and promotes their internalization, thu...

journal_title：Journal of cell science

authors： Ma L,Pei G

更新日期：2007-01-15 00:00:00

abstract：:What forces drive chromosome segregation remains one of the most challenging questions in cell division. Even though the duration of anaphase is short, it is of utmost importance for genome fidelity that no mistakes are made. Seminal studies in model organisms have revealed different mechanisms operating during chromo...

journal_title：Journal of cell science

authors： Vukušić K,Buđa R,Tolić IM

更新日期：2019-09-16 00:00:00

abstract：:In the developing Drosophila eye, the centrioles of the differentiating retinal cells are not surrounded by the microtubule-nucleating γ-tubulin, suggesting that they are unable to organize functional microtubule-organizing centers. Consistent with this idea, Cnn and Spd-2, which are involved in γ-tubulin recruitment,...

journal_title：Journal of cell science

authors： Riparbelli MG,Persico V,Gottardo M,Callaini G

更新日期：2018-02-22 00:00:00

abstract：:Neural stem cells (NSCs) typically show efficient self-renewal and selective differentiation. Their invasion potential, however, is not well studied. In this study, Sox2-positive NSCs from the E14.5 rat cortex were found to be non-invasive and showed only limited migration in vitro. By contrast, FGF2-expanded NSCs sho...

journal_title：Journal of cell science

authors： Sailer MH,Gerber A,Tostado C,Hutter G,Cordier D,Mariani L,Ritz MF

更新日期：2013-08-15 00:00:00

abstract：:Transcriptional activation of the major histocompatibility complex (MHC) by IFNgamma is a key step in cell-mediated immunity. At an early stage of IFNgamma induction, chromatin carrying the entire MHC locus loops out from the chromosome 6 territory. We show here that JAK/STAT signalling triggers this higher-order chro...

journal_title：Journal of cell science

authors： Christova R,Jones T,Wu PJ,Bolzer A,Costa-Pereira AP,Watling D,Kerr IM,Sheer D

更新日期：2007-09-15 00:00:00

abstract：:Borrelia burgdorferi is the causative agent of Lyme disease, a multisystemic disorder affecting primarily skin, joints and nervous system. Successful internalization and intracellular processing of borreliae by immune cells like macrophages is decisive for the outcome of a respective infection. Here, we use for the fi...

journal_title：Journal of cell science

authors： Klose M,Scheungrab M,Luckner M,Wanner G,Linder S

更新日期：2020-12-30 00:00:00

abstract：:The redox status of human spermatozoa was found to have a profound influence on the fertilizing potential of these cells in association with qualitative and quantitative changes in the patterns of tyrosine phosphorylation. In general, oxidizing conditions enhanced tyrosine phosphorylation and stimulated sperm function...

journal_title：Journal of cell science

authors： Aitken RJ,Paterson M,Fisher H,Buckingham DW,van Duin M

更新日期：1995-05-01 00:00:00

abstract：:alpha3beta1-integrin is abundantly expressed in the epidermis, and in mice, ablation of the alpha3 gene results in embryonic defects and perinatal lethality. To determine the role of alpha3-integrin in adult skin development, we grafted skin from newborn alpha3-integrin-deficient mice on to ICRF nu/nu recipients. We r...

journal_title：Journal of cell science

authors： Conti FJ,Rudling RJ,Robson A,Hodivala-Dilke KM

更新日期：2003-07-01 00:00:00

abstract：:In fission yeast, Mid1p, a major determinant for division plane position, defines a medial cortical compartment where it recruits myosin II at the onset of mitosis to initiate contractile ring assembly. How Mid1p is restricted to the medial cortex is unknown. We report here that in a pom1 polarity mutant, which displa...

journal_title：Journal of cell science

authors： Celton-Morizur S,Racine V,Sibarita JB,Paoletti A

更新日期：2006-11-15 00:00:00

abstract：:Plectin, a cytolinker protein greater than 500 kDa in size, has an important role as a mechanical stabiliser of cells. It interlinks the various cytoskeletal filament systems and anchors intermediate filaments to peripheral junctional complexes. In addition, there is increasing evidence that plectin acts as a scaffold...

journal_title：Journal of cell science

authors： Gregor M,Zeöld A,Oehler S,Marobela KA,Fuchs P,Weigel G,Hardie DG,Wiche G

更新日期：2006-05-01 00:00:00