Lumican is required for neutrophil extravasation following corneal injury and wound healing.

Abstract:

:An important aspect of wound healing is the recruitment of neutrophils to the site of infection or tissue injury. Lumican, an extracellular matrix component belonging to the small leucine rich proteoglycan (SLRP) family, is one of the major keratan sulfate proteoglycans (KSPGs) within the corneal stroma. Increasing evidence indicates that lumican can serve as a regulatory molecule for several cellular processes, including cell proliferation and migration. In the present study, we addressed the role of lumican in the process of extravasation of polymorphonuclear leukocytes (PMNs) during the early inflammatory phase present in the healing of the corneal epithelium following debridement. We used Lum(-/-) mice and a novel transgenic mouse, Lum(-/-),Kera-Lum, which expresses lumican only in the corneal stroma, to assess the role of lumican in PMN extravasation into injured corneas. Our results showed that PMNs did not readily invade injured corneas of Lum(-/-) mice and this defect was rescued by the expression of lumican in the corneas of Lum(-/-),Kera-Lum mice. The presence of lumican in situ facilitates PMN infiltration into the peritoneal cavity in casein-induced inflammation. Our findings are consistent with the notion that in addition to regulating the collagen fibril architecture, lumican acts to aid neutrophil recruitment and invasion following corneal damage and inflammation.

journal_name

J Cell Sci

journal_title

Journal of cell science

authors

Hayashi Y,Call MK,Chikama T,Liu H,Carlson EC,Sun Y,Pearlman E,Funderburgh JL,Babcock G,Liu CY,Ohashi Y,Kao WW

doi

10.1242/jcs.068221

subject

Has Abstract

pub_date

2010-09-01 00:00:00

pages

2987-95

issue

Pt 17

eissn

0021-9533

issn

1477-9137

pii

jcs.068221

journal_volume

123

pub_type

杂志文章
  • Electron-microscope study of Dictyostelium discoideum plasma membrane and its modifications during and after phagocytosis.

    abstract::The study of plasma membrane and phagosome membrane of Dictyostelium discoideum was performed using 2 lectins: concanavalin A (Con A) and Wheat Germ Agglutinin (WGA), and 2 markers of anionic sites: colloidal iron hydroxide (CIH) and cationized ferritin (CF). These labellings were applied to fixed partially broken cel...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Ryter A,Hellio R

    更新日期:1980-02-01 00:00:00

  • CCAR-1 affects hemidesmosome biogenesis by regulating unc-52/perlecan alternative splicing in the C. elegans epidermis.

    abstract::Hemidesmosomes are epithelial-specific attachment structures that maintain tissue integrity and resist tension. Despite their importance, how hemidesmosomes are regulated at the post-transcriptional level is poorly understood. Caenorhabditiselegans hemidesmosomes (CeHDs) have a similar structure and composition to the...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.214379

    authors: Fu R,Zhu Y,Jiang X,Li Y,Zhu M,Dong M,Huang Z,Wang C,Labouesse M,Zhang H

    更新日期:2018-06-11 00:00:00

  • Sphingomyelin synthase-related protein SMSr is a suppressor of ceramide-induced mitochondrial apoptosis.

    abstract::Cells synthesize ceramides in the endoplasmic reticulum (ER) as precursors for sphingolipids to form an impermeable plasma membrane. As ceramides are engaged in apoptotic pathways, cells would need to monitor their levels closely to avoid killing themselves during sphingolipid biosynthesis. How this is accomplished re...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.138933

    authors: Tafesse FG,Vacaru AM,Bosma EF,Hermansson M,Jain A,Hilderink A,Somerharju P,Holthuis JC

    更新日期:2014-01-15 00:00:00

  • Developmentally and environmentally regulated expression of gamone 1: the trigger molecule for sexual reproduction in Blepharisma japonicum.

    abstract::Sexual reproduction (conjugation) in protozoan ciliates is induced by specific cell-cell interactions between cells of complementary mating types. The ancestral ciliate Blepharisma japonicum has two mating types, I and II. The substances that act as signaling molecules in this extracellular interaction for conjugation...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.02359

    authors: Sugiura M,Kawahara S,Iio H,Harumoto T

    更新日期:2005-06-15 00:00:00

  • A method for determining the periodicity of a troponin component in isolated insect flight muscle thin filaments by gold/Fab labelling.

    abstract::Insect flight muscle has a large component (Tn-H) in the tropomyosin-troponin complex that is not present in vertebrate striated muscle thin filaments. Tn-H is shown by gold/Fab labelling to be present at regular intervals in insect flight muscle thin filaments. The Fab fragment of a monoclonal antibody to Tn-H was co...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Newman R,Butcher GW,Bullard B,Leonard KR

    更新日期:1992-03-01 00:00:00

  • The chemotactic and mitogenic effects of platelet-derived growth factor-BB on rat aorta smooth muscle cells are inhibited by basic fibroblast growth factor.

    abstract::In response to endovascular injury, platelet-derived growth factor-BB (PDGF-BB) and basic fibroblast growth factor (bFGF) are released locally and modulate vascular smooth muscle cells (SMC) proliferation and migration within the vascular wall. The aim of the present in vitro study was to determine how rat aorta SMC r...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Facchiano A,De Marchis F,Turchetti E,Facchiano F,Guglielmi M,Denaro A,Palumbo R,Scoccianti M,Capogrossi MC

    更新日期:2000-08-01 00:00:00

  • TRAPPII is required for cleavage furrow ingression and localization of Rab11 in dividing male meiotic cells of Drosophila.

    abstract::Although membrane addition is crucial for cytokinesis in many animal cell types, the specific mechanisms supporting cleavage furrow ingression are not yet understood. Mutations in the gene brunelleschi (bru), which encodes the Drosophila ortholog of the yeast Trs120p subunit of TRAPPII, cause failure of furrow ingress...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.054536

    authors: Robinett CC,Giansanti MG,Gatti M,Fuller MT

    更新日期:2009-12-15 00:00:00

  • Neutrophil behaviour in the presence of protease inhibitors.

    abstract::Neutrophil leucocytes circulate in plasma, which contains broad-spectrum antiproteases such as alpha-2-macroglobulin and alpha-1-antitrypsin, and move into environments in which antiproteases are present. Plasma antiproteases significantly reduce the adhesiveness of leucocytes in both static and dynamic adhesion assay...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Forrester JV,Lackie JM,Brown AF

    更新日期:1983-01-01 00:00:00

  • Connexin-43 K63-polyubiquitylation on lysines 264 and 303 regulates gap junction internalization.

    abstract::Gap junctions (GJs) assembled from connexin (Cx) proteins allow direct cell-cell communication. While phosphorylation is known to regulate multiple GJ functions, much less is known about the role of ubiquitin in these processes. Using ubiquitylation-type-specific antibodies and Cx43 lysine-to-arginine mutants we show ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.204321

    authors: Kells-Andrews RM,Margraf RA,Fisher CG,Falk MM

    更新日期:2018-08-09 00:00:00

  • Variation in cytoplasmic microtubule organization and spindle length between the two forms of the dimorphic fungus Candida albicans.

    abstract::Candida albicans is a dimorphic fungus capable of growing as a budding yeast and as a filamentous hypha. We have used the technique of immunofluorescence to study the changes in the microtubule cytoskeleton during the cell cycle in both growth forms. This approach has revealed the presence of an extensive system of mi...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Barton R,Gull K

    更新日期:1988-10-01 00:00:00

  • Molecular basis for MMP9 induction and disruption of epithelial cell-cell contacts by galectin-3.

    abstract::Dynamic modulation of the physical contacts between neighboring cells is integral to epithelial processes such as tissue repair and cancer dissemination. Induction of matrix metalloproteinase (MMP) activity contributes to the disassembly of intercellular junctions and the degradation of the extracellular matrix, thus ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.148510

    authors: Mauris J,Woodward AM,Cao Z,Panjwani N,Argüeso P

    更新日期:2014-07-15 00:00:00

  • Molecular determinants for differential membrane trafficking of PMCA1 and PMCA2 in mammalian hair cells.

    abstract::The plasma membrane Ca2+-ATPase-2 (PMCA2) is expressed in stereocilia of hair cells of the inner ear, whereas PMCA1 is expressed in the basolateral plasma membrane of hair cells. Both extrude excess Ca2+ from the cytosol. They are predicted to contain ten membrane-spanning segments, two large cytoplasmic loops as well...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.03030

    authors: Grati M,Aggarwal N,Strehler EE,Wenthold RJ

    更新日期:2006-07-15 00:00:00

  • The role of mechanical stimuli in the vascular differentiation of mesenchymal stem cells.

    abstract::Mesenchymal stem cells (MSCs) are among the most promising and suitable stem cell types for vascular tissue engineering. Substantial effort has been made to differentiate MSCs towards vascular cell phenotypes, including endothelial cells and smooth muscle cells (SMCs). The microenvironment of vascular cells not only c...

    journal_title:Journal of cell science

    pub_type: 杂志文章,评审

    doi:10.1242/jcs.167783

    authors: Dan P,Velot É,Decot V,Menu P

    更新日期:2015-07-15 00:00:00

  • Regulation of the acquisition of meiotic competence in the mouse: changes in the subcellular localization of cdc2, cyclin B1, cdc25C and wee1, and in the concentration of these proteins and their transcripts.

    abstract::During their development, mammalian oocytes acquire the ability to resume meiosis. We demonstrate that the concentration of p34cdc2 increases during the acquisition of meiotic competence, as determined by immunoblotting, whereas the concentration of cyclin B1 decreases. Laser-scanning confocal microscopy corroborated ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Mitra J,Schultz RM

    更新日期:1996-09-01 00:00:00

  • Inversion heterozygosity in females of the newt Notophthalmus viridescens and its influence on chiasma distribution.

    abstract::In a wild population of the American newt Notophthalmus viridescens 15 females out of a total of 94 were found to be heterozygous for a paracentric inversion which includes almost the whole of the longer arm of the smallest chromosome (XI). The inversion was recognized in preparations of lampbrush chromosomes because ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Hartley SE,Callan HG

    更新日期:1977-04-01 00:00:00

  • Flagellum elongation is required for correct structure, orientation and function of the flagellar pocket in Trypanosoma brucei.

    abstract::In trypanosomes, the flagellum is rooted in the flagellar pocket, a surface micro-domain that is the sole site for endocytosis and exocytosis. By analysis of anterograde or retrograde intraflagellar transport in IFT88RNAi or IFT140RNAi mutant cells, we show that elongation of the new flagellum is not required for flag...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.035626

    authors: Absalon S,Blisnick T,Bonhivers M,Kohl L,Cayet N,Toutirais G,Buisson J,Robinson D,Bastin P

    更新日期:2008-11-15 00:00:00

  • cdc2 kinase-induced destabilization of MAP2-coated microtubules in Xenopus egg extracts.

    abstract::During the interphase to metaphase transition, microtubules are destabilized by a cdc2 kinase-dependent phosphorylation event. This destabilization is due to a dramatic increase in the rate at which each growing microtubule starts to shrink (catastrophe rate). In principle, this could be brought about by lowering the ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Faruki S,Dorée M,Karsenti E

    更新日期:1992-01-01 00:00:00

  • A developmentally regulated ARF-like 5 protein (ARL5), localized to nuclei and nucleoli, interacts with heterochromatin protein 1.

    abstract::ARF-like proteins (ARLs) are distinct group of members of the ARF family of Ras-related GTPases. Although ARLs are very similar in primary structure to ARFs, their functions remain unclear. We cloned mouse (m) and human (h) ARL5 cDNAs to characterize the protein products and their molecular properties. mARL5 mRNA was ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.00123

    authors: Lin CY,Li CC,Huang PH,Lee FJ

    更新日期:2002-12-01 00:00:00

  • The development of lampbrush chromosome-type transcription in the early diplotene oocytes of Xenopus laevis: an electron-microscope analysis.

    abstract::Oocytes of Xenopus laevis in pachytene and early diplotene of meiosis have been studied using the Miller spreading technique. Transcription first appears in germinal vesicles 25-40 micrometers in diameter, when the oocyte is in early diplotene. Transcription at this stage consists of arrays of short RNP transcripts, i...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Hill RS,Macgregor HC

    更新日期:1980-08-01 00:00:00

  • Myoferlin regulation by NFAT in muscle injury, regeneration and repair.

    abstract::Ferlin proteins mediate membrane-fusion events in response to Ca(2+). Myoferlin, a member of the ferlin family, is required for normal muscle development, during which it mediates myoblast fusion. We isolated both damaged and intact myofibers from a mouse model of muscular dystrophy using laser-capture microdissection...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.065375

    authors: Demonbreun AR,Lapidos KA,Heretis K,Levin S,Dale R,Pytel P,Svensson EC,McNally EM

    更新日期:2010-07-15 00:00:00

  • Large heterogeneity of mitochondrial DNA transcription and initiation of replication exposed by single-cell imaging.

    abstract::Mitochondrial DNA (mtDNA) replication and transcription are crucial for cell function, but these processes are poorly understood at the single-cell level. We describe a novel fluorescence in situ hybridization protocol, called mTRIP (mitochondrial transcription and replication imaging protocol), that reveals simultane...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.114322

    authors: Chatre L,Ricchetti M

    更新日期:2013-02-15 00:00:00

  • Mapping cell-glass contacts of Dictyostelium amoebae by total internal reflection aqueous fluorescence overcomes a basic ambiguity of interference reflection microscopy.

    abstract::The widespread ability of eukaryotic cells to produce thin cytoplasmic sheets or lamellae 100-200 nm thick can give rise to uncertainties in the interpretation of interference reflection microscopy (IRM) images when cell-substratum topography is the key interest. If allowed to spread upon a poly-L-lysine-coated surfac...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Todd I,Mellor JS,Gingell D

    更新日期:1988-01-01 00:00:00

  • Recurrent deletions of ULK4 in schizophrenia: a gene crucial for neuritogenesis and neuronal motility.

    abstract::Although many pathogenic copy number variations (CNVs) are associated with neuropsychiatric diseases, few of them have been functionally characterised. Here we report multiple schizophrenia cases with CNV abnormalities specific to unc-51-like kinase 4 (ULK4), a serine/threonine kinase gene. Deletions spanning exons 21...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.137604

    authors: Lang B,Pu J,Hunter I,Liu M,Martin-Granados C,Reilly TJ,Gao GD,Guan ZL,Li WD,Shi YY,He G,He L,Stefánsson H,St Clair D,Blackwood DH,McCaig CD,Shen S

    更新日期:2014-02-01 00:00:00

  • Lentivirus-mediated transduction of connexin cDNAs shows level- and isoform-specific alterations in insulin secretion of primary pancreatic beta-cells.

    abstract::We have generated novel lentiviral vectors to integrate various connexin cDNAs into primary, non-dividing cells. We have used these vectors to test whether proper control of insulin secretion depends on a specific connexin isoform and/or on its level of expression. We have observed that transduced connexin32, connexin...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.00442

    authors: Caton D,Calabrese A,Mas C,Serre-Beinier V,Charollais A,Caille D,Zufferey R,Trono D,Meda P

    更新日期:2003-06-01 00:00:00

  • A caveolin-3 mutant that causes limb girdle muscular dystrophy type 1C disrupts Src localization and activity and induces apoptosis in skeletal myotubes.

    abstract::Caveolins are membrane proteins that are the major coat proteins of caveolae, specialized lipid rafts in the plasma membrane that serve as scaffolding sites for many signaling complexes. Among the many signaling molecules associated with caveolins are the Src tyrosine kinases, whose activation regulates numerous cellu...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.00806

    authors: Smythe GM,Eby JC,Disatnik MH,Rando TA

    更新日期:2003-12-01 00:00:00

  • Meeting report--Imaging the Cell.

    abstract::Every two years, the French Society for Cell Biology (SBCF) organises an international meeting called 'Imaging the Cell'. This year, the 8th edition was held on 24-26 June 2015 at University of Bordeaux Campus Victoire in the city of Bordeaux, France, a UNESCO World Heritage site. Over the course of three days, the me...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.180042

    authors: Moreau V,Cordelières FP,Poujol C,Sagot I,Saltel F

    更新日期:2015-11-01 00:00:00

  • Histochemical and ultrastructural characteristics of a cell line from human bone-marrow stroma.

    abstract::Morphological, enzymic and antigenic data are presented regarding a human bone-marrow stromal cell line maintained for 10 months and subcultured weekly. The main characteristics are a fibroblastoid morphology, diffuse growth in collagen gels, no colony formation in soft gel media, contact inhibition of growth and conv...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Lanotte M,Allen TD,Dexter TM

    更新日期:1981-08-01 00:00:00

  • Formation of multivesicular endosomes in Dictyostelium.

    abstract::Multivesicular endosomes are present in virtually every eucaryotic cell, where they arise by intra-endosomal budding of the limiting endosomal membrane. Some genetic diseases such as Chediak-Higashi syndrome are characterized by enlarged membrane-filled endosomes. The same altered endosomal morphology can be observed ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.01524

    authors: Marchetti A,Mercanti V,Cornillon S,Alibaud L,Charette SJ,Cosson P

    更新日期:2004-12-01 00:00:00

  • Saccharomyces cerevisiae mutants lacking a functional vacuole are defective for aspects of the pheromone response.

    abstract::The end1 mutant belongs to a group of four vacuolar protein sorting mutants (class C vps) that lack a morphologically distinguishable and functional vacuole. These mutants share several other phenotypes, such as the inability to grow at 37 degrees C or on nonfermentable carbon sources. We show that, as in the case of ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Dulić V,Riezman H

    更新日期:1990-11-01 00:00:00

  • Stabilization and activation of p53 induced by Cdk5 contributes to neuronal cell death.

    abstract::The p53 tumor suppressor protein is a key regulator of cellular functions including responses to numerous stress signals, and triggers apoptosis in many cell types, including neurons. The major mechanisms known to regulate p53 stabilization and activation include phosphorylation and ubiquitin ligase-mediated proteasom...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.03468

    authors: Lee JH,Kim HS,Lee SJ,Kim KT

    更新日期:2007-07-01 00:00:00