DNA damage response clamp 9-1-1 promotes assembly of ZMM proteins for formation of crossovers and synaptonemal complex.

abstract：:Present in organisms ranging from yeast to man, homologues of the Saccharomyces cerevisiae ubiquitin-conjugating enzyme CDC34 have been shown to play important roles in the regulation of cell cycle progression and checkpoint function. Here we analyze the expression and intracellular localization of endogenous CDC34 du...

journal_title：Journal of cell science

authors： Reymond F,Wirbelauer C,Krek W

更新日期：2000-05-01 00:00:00

abstract：:We have investigated the role in macrophage lysosomal enzyme release of arachidonate metabolites, extracellular divalent cations and cyclic AMP (cAMP) which modulate secretion in other cell types. Lysosomal enzyme secretion induced by zymosan was accompanied by release of malondialdehyde (MDA), which is derived from a...

journal_title：Journal of cell science

authors： McMillan RM,Macintyre DE,Beesley JE,Gordon JL

更新日期：1980-08-01 00:00:00

abstract：:Junction dynamics of endothelial cells are based on the integration of signal transduction, cytoskeletal remodeling and contraction, which are necessary for the formation and maintenance of monolayer integrity, but also enable repair and regeneration. The VE-cadherin-catenin complex forms the molecular basis of the ad...

journal_title：Journal of cell science

authors： Cao J,Schnittler H

更新日期：2019-01-03 00:00:00

abstract：:Syntaxin 1C (STX1C), produced by alternative splicing of the stx1A gene, is a soluble syntaxin lacking a SNARE domain and a transmembrane domain. It is unclear how soluble syntaxin can control intracellular membrane trafficking. We found that STX1C affected microtubule (MT) dynamics through its tubulin-binding domain ...

journal_title：Journal of cell science

authors： Nakayama T,Kamiguchi H,Akagawa K

更新日期：2012-02-15 00:00:00

abstract：:Keratin 8 is a type II intermediate filament protein found in simple epithelia. We have introduced a 12 kb DNA fragment of the human K8 locus into the germ line of mice. The transgene, containing 1.1 kb of 5' flanking sequences, 7.7 kb corresponding to the body of the gene and 3.2 kb of 3' flanking sequences, was expr...

journal_title：Journal of cell science

authors： Casanova L,Bravo A,Were F,Ramírez A,Jorcano JJ,Vidal M

更新日期：1995-02-01 00:00:00

abstract：:Intracellular pH has been measured by laser microspectrofluorimetry, using the pH-sensitive dyes SNARF-1, SNARF-calcein and SNARF-1-dextran. By this technique it was possible to accurately determine pH in volumes as small as 0.5 x 0.5 x 1 microns 3. The probes were loaded into the cells either by diffusion of their ac...

journal_title：Journal of cell science

authors： Seksek O,Bolard J

更新日期：1996-01-01 00:00:00

abstract：:We have used MPM-2, a monoclonal antibody raised against mitotic HeLa cells, to stain a Drosophila cell line, whole mounts of Drosophila embryos, and sectioned tissue from embryonic and larval stages of development. MPM-2 recognizes a major phosphoprotein of approximately 125 X 10(3) Mr in Drosophila tissue culture ce...

journal_title：Journal of cell science

authors： Millar SE,Freeman M,Glover DM

更新日期：1987-02-01 00:00:00

abstract：:Disruption of the sarcoglycan complex leads to muscle membrane instability and muscular dystrophy in humans and mice. Through the dystrophin glycoprotein complex, sarcoglycan participates in connecting the internal cytoskeleton to the membrane and the extracellular matrix. Integrin alpha7beta1 is also a transmembrane ...

journal_title：Journal of cell science

authors： Allikian MJ,Hack AA,Mewborn S,Mayer U,McNally EM

更新日期：2004-08-01 00:00:00

abstract：:The family of integrin transmembrane receptors is essential for the normal function of multicellular organisms by facilitating cell-extracellular matrix adhesion. The vitronectin-binding integrin αVβ5 localizes to focal adhesions (FAs) as well as poorly characterized flat clathrin lattices (FCLs). Here, we show that, ...

journal_title：Journal of cell science

authors： Zuidema A,Wang W,Kreft M,Te Molder L,Hoekman L,Bleijerveld OB,Nahidiazar L,Janssen H,Sonnenberg A

更新日期：2018-11-05 00:00:00

abstract：:The serine/threonine protein phosphatase-1 (PP1) complex is a key regulator of the cell cycle. However, the redundancy of PP1 isoforms and the lack of specific inhibitors have hampered studies on the global role of PP1 in cell cycle progression in vertebrates. Here, we show that the overexpression of nuclear inhibitor...

journal_title：Journal of cell science

authors： Winkler C,De Munter S,Van Dessel N,Lesage B,Heroes E,Boens S,Beullens M,Van Eynde A,Bollen M

更新日期：2015-12-15 00:00:00

abstract：:In Saccharomyces cerevisiae, DNA replication stress activates the replication checkpoint, which slows S-phase progression, stabilizes slowed or stalled replication forks, and relieves inhibition of the ribonucleotide reductase (RNR) complex. To identify novel genes that promote cellular viability after replication str...

journal_title：Journal of cell science

authors： Woolstencroft RN,Beilharz TH,Cook MA,Preiss T,Durocher D,Tyers M

更新日期：2006-12-15 00:00:00

abstract：:Focal adhesion kinase is a recently characterized tyrosine kinase that is concentrated at focal contacts in cultured cells. It is thought to play an important role in the regulation of the integrin-based signal transduction mechanism involved in the assembly of this membrane specialization. In this study, we examined ...

journal_title：Journal of cell science

authors： Baker LP,Daggett DF,Peng HB

更新日期：1994-06-01 00:00:00

abstract：:Insulin-like growth factor-1 (IGF-1) has been shown to induce skeletal muscle hypertrophy, to prevent the loss of muscle mass with ageing and to improve the muscle phenotype of dystrophic mice. We previously developed a model of IGF-1-induced hypertrophy of human myotubes, in which hypertrophy was not only characteriz...

journal_title：Journal of cell science

authors： Jacquemin V,Butler-Browne GS,Furling D,Mouly V

更新日期：2007-02-15 00:00:00

abstract：:Pin1/Ess1p is a highly conserved WW domain-containing peptidyl-prolyl isomerase (PPIase); its WW domain binds specifically to phospho-Ser/Thr-Pro sequences and its catalytic domain isomerizes phospho-Ser/Thr-Pro bonds. Pin1 PPIase activity can alter protein conformation in a phosphorylation-dependent manner and/or pro...

journal_title：Journal of cell science

authors： Huang HK,Forsburg SL,John UP,O'Connell MJ,Hunter T

更新日期：2001-10-01 00:00:00

abstract：:The development of DNA microarrays to study simultaneously the level of mRNA expressed from thousands of genes offers great promise to cell biologists. Microarrays can be used to gain detailed information about transcriptional changes involved in a specific pathway, potentially leading to the identification of novel c...

journal_title：Journal of cell science

authors： Schulze A,Downward J

更新日期：2000-12-01 00:00:00

abstract：:A specific mechanism for the intracellular translocation of nonvesicle-associated proteins is proposed. This movement machinery is based on the assumption that the cytoskeleton represents an interconnected network of filamentous macromolecules, which extends over the entire cytoplasm. Diffusion along the filaments pro...

journal_title：Journal of cell science

authors： Shafrir Y,ben-Avraham D,Forgacs G

更新日期：2000-08-01 00:00:00

abstract：:Calnexin and calreticulin are related proteins that comprise an ER chaperone system that ensures the proper folding and quality control of newly synthesized glycoproteins. The specificity for glycoproteins is conferred by a lectin site that recognizes an early oligosaccharide processing intermediate on the folding gly...

journal_title：Journal of cell science

authors： Williams DB

更新日期：2006-02-15 00:00:00

abstract：:The intraerythrocytic developmental cycle of Plasmodium falciparum is completed with the release of up to 32 invasive daughter cells, the merozoites, into the blood stream. Before release, the final step of merozoite development is the assembly of the cortical pellicle, a multi-layered membrane structure. This unique ...

journal_title：Journal of cell science

authors： Kono M,Heincke D,Wilcke L,Wong TW,Bruns C,Herrmann S,Spielmann T,Gilberger TW

更新日期：2016-02-15 00:00:00

abstract：:In fibroblasts, platelet-derived growth factor receptor alpha (PDGFRα) is upregulated during growth arrest and compartmentalized to the primary cilium. PDGF-AA mediated activation of the dimerized ciliary receptor produces a phosphorylation cascade through the PI3K-AKT and MEK1/2-ERK1/2 pathways leading to the activat...

journal_title：Journal of cell science

authors： Clement DL,Mally S,Stock C,Lethan M,Satir P,Schwab A,Pedersen SF,Christensen ST

更新日期：2013-02-15 00:00:00

abstract：:The vacuolar H(+)-ATPase (V-ATPase) establishes pH gradients along secretory and endocytic pathways. Progressive acidification is essential for proteolytic processing of prohormones and aggregation of soluble content proteins. The V-ATPase V(0) subunit is thought to have a separate role in budding and fusion events. P...

journal_title：Journal of cell science

authors： Sobota JA,Bäck N,Eipper BA,Mains RE

更新日期：2009-10-01 00:00:00

abstract：:The signal recognition particle (SRP) is a ribonucleoprotein machine that controls the translation and intracellular sorting of membrane and secreted proteins. The SRP contains a core RNA subunit with which six proteins are assembled. Recent work in both yeast and mammalian cells has identified the nucleolus as a poss...

journal_title：Journal of cell science

authors： Sommerville J,Brumwell CL,Politz JC,Pederson T

更新日期：2005-03-15 00:00:00

abstract：:MACF1 (microtubule actin crosslinking factor), also called ACF7 (actin crosslinking family 7) is a cytoskeletal linker protein that can associate with both actin filaments and microtubules. We have identified a novel alternatively spliced isoform of MACF1. We named this isoform MACF1b and renamed the original isoform ...

journal_title：Journal of cell science

authors： Lin CM,Chen HJ,Leung CL,Parry DA,Liem RK

更新日期：2005-08-15 00:00:00

abstract：:Excitatory amino acid transporter type 3 (EAAT3, also known as SLC1A1) is a high-affinity, Na(+)-dependent glutamate carrier that localizes primarily within the cell and at the apical plasma membrane. Although previous studies have reported proteins and sequence regions involved in EAAT3 trafficking, the detailed mole...

journal_title：Journal of cell science

authors： Su JF,Wei J,Li PS,Miao HH,Ma YC,Qu YX,Xu J,Qin J,Li BL,Song BL,Xu ZP,Luo J

更新日期：2016-08-15 00:00:00

abstract：:Targeting of the NG2 proteoglycan to cellular retraction fibers was studied by expressing mutant NG2 molecules lacking specific structural elements of the proteoglycan. Both the cytoplasmic domain and the chondroitin sulfate chain of NG2 appear to have roles in sorting NG2 to subcellular microdomains destined to becom...

journal_title：Journal of cell science

authors： Stallcup WB,Dahlin-Huppe K

更新日期：2001-06-01 00:00:00

abstract：:Cytoplasmic linker proteins (CLIPs) bind to microtubules and are proposed to link this cytoskeletal network to other intracellular structures. We are interested in CLIP-115, since this protein is enriched in neuronal dendrites and may operate in the control of brain-specific organelle translocations. Each CLIP monomer...

journal_title：Journal of cell science

authors： Hoogenraad CC,Akhmanova A,Grosveld F,De Zeeuw CI,Galjart N

更新日期：2000-06-01 00:00:00

abstract：:Basonuclin is a zinc finger protein mainly expressed in keratinocytes of the basal layer of epidermis and the outer root sheath of hair follicles. It is also found in abundance in the germ cells of testis and ovary. In cultured keratinocytes, basonuclin is associated with chromatin in all phases of the cell cycle, inc...

journal_title：Journal of cell science

authors： Tseng H,Biegel JA,Brown RS

更新日期：1999-09-01 00:00:00

abstract：:Most nonmuscle cells of higher vertebrates contain two different actin isoforms, beta- and gamma-cytoplasmic actin. The beta-isoform is with few exceptions the predominant isoform in nonmuscle cells and tissues. Perturbation of the beta:gamma ratio has been shown to affect the organization of bundled actin filaments i...

journal_title：Journal of cell science

authors： Höfer D,Ness W,Drenckhahn D

更新日期：1997-03-01 00:00:00

abstract：:This study focuses on the effect of static and dynamic mechanical compression on the biosynthetic activity of chondrocytes cultured within agarose gel. Chondrocyte/agarose disks (3 mm diameter) were placed between impermeable platens and subjected to uniaxial unconfined compression at various times in culture (2-43 da...

journal_title：Journal of cell science

authors： Buschmann MD,Gluzband YA,Grodzinsky AJ,Hunziker EB

更新日期：1995-04-01 00:00:00

abstract：:Collapsin response mediator proteins (CRMPs) mediate signal transduction of neurite outgrowth and axonal guidance during neuronal development. Voltage-gated Ca(2+) channels and interacting proteins are essential in neuronal signaling and synaptic transmission during this period. We recently identified the presynaptic ...

journal_title：Journal of cell science

authors： Chi XX,Schmutzler BS,Brittain JM,Wang Y,Hingtgen CM,Nicol GD,Khanna R

更新日期：2009-12-01 00:00:00

abstract：:Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) triggers apoptosis selectively in tumor cells through interaction with TRAIL-R1/DR4 or TRAIL-R2/DR5 and this process is considered a promising avenue for cancer treatment. TRAIL resistance, however, is frequently encountered and hampers anti-cancer ...

journal_title：Journal of cell science

authors： Azijli K,Yuvaraj S,Peppelenbosch MP,Würdinger T,Dekker H,Joore J,van Dijk E,Quax WJ,Peters GJ,de Jong S,Kruyt FA

更新日期：2012-10-01 00:00:00