Abstract：
:The formation and extension of filopodia in response to an extracellular stimulus by guidance cues determine the path of growth cone advance. Actin-filament bundling and actin polymerization at the tips supply the driving force behind the formation and elongation. We tried to clarify how signals in response to extracellular cues are transformed to induce filopodial generation and extension. Observations on the formation process of filopodia at growth cones in the neuroblastoma cell line NG108 showed that WAVE (WASP (Wiskott-Aldrich syndrome protein)-family verprolin homologous protein) isoforms played crucial and distinct roles in this process. WAVE1 was continuously distributed along the leading edge only and was not found in the filopodia. WAVE2 and WAVE3 discretely localized at the initiation sites of microspikes on the leading edge and also concentrated at the tips of protruding filopodia. We further found that WAVE isoforms localized at the filopodial tips through SHD (SCAR homology domain), next to its leucine zipper-like motif. Furthermore, time-lapse observations of filopodial formation in living cells showed that WAVE2 and WAVE3 were continuously expressed at the tips of filopodia during elongation. These results indicate that WAVE2 or WAVE3 may guide the actin bundles into the filopodia and promote actin assembly at the tips.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Nozumi M,Nakagawa H,Miki H,Takenawa T,Miyamoto Sdoi
10.1242/jcs.00233keywords:
subject
Has Abstractpub_date
2003-01-15 00:00:00pages
239-46issue
Pt 2eissn
0021-9533issn
1477-9137journal_volume
116pub_type
杂志文章abstract：:Host/symbiont specificity has been investigated in non-symbiotic and aposymbiotic brown and green hydra infected with various free-living and symbiotic species and strains of Chlorella and Chlorococcum. Morphology and ultrastructure of the symbioses obtained have been compared. Aposymbiotic Swiss Hydra viridis and Jap...
journal_title：Journal of cell science
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更新日期：2007-06-01 00:00:00
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更新日期：2019-03-07 00:00:00
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doi：
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更新日期：2001-05-01 00:00:00
