Chromosomes attain a metaphase position on half-spindles in the absence of an opposing spindle pole.

abstract：:Tau is a group of neuronal microtubule-associated proteins that are formed by alternative mRNA splicing and accumulate in neurofibrillary tangles in Alzheimer's disease (AD) brain. Tau plays a key role in regulating microtubule dynamics, axonal transport and neurite outgrowth, and all these functions of tau are modula...

journal_title：Journal of cell science

authors： Johnson GV,Stoothoff WH

更新日期：2004-11-15 00:00:00

abstract：:The tumor suppressor p53 is activated in response to many forms of cellular stress leading to cell cycle arrest, senescence or apoptosis. Appropriate sub-cellular localization is essential for modulating p53 function. We recently showed that p53 localizes to the nucleolus after proteasome inhibition with MG132 and thi...

journal_title：Journal of cell science

authors： Karni-Schmidt O,Zupnick A,Castillo M,Ahmed A,Matos T,Bouvet P,Cordon-Cardo C,Prives C

更新日期：2008-12-15 00:00:00

abstract：:When tumour cells are fused with normal ones, malignancy is suppressed. It has been shown that this suppression is associated with the imposition on the hybrid cell of the terminal differentiation programme of the normal parent cell. We report here the consequences of imposing the synthesis of keratin 1 and keratin 10...

journal_title：Journal of cell science

authors： Harris H,Rawlins J,Sharps J

更新日期：1996-09-01 00:00:00

abstract：:Annexins are cytosolic phospholipid-binding proteins that can be found on the outer leaflet of the plasma membrane. The extracellular functions of annexin include modulating fibrinolysis activity and cell migration. Despite having well-described extracellular functions, the mechanism of annexin transport from the cyto...

journal_title：Journal of cell science

authors： Stewart SE,Ashkenazi A,Williamson A,Rubinsztein DC,Moreau K

更新日期：2018-07-19 00:00:00

abstract：:SNARE [soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) receptor] proteins control the membrane-fusion events of eukaryotic membrane-trafficking pathways. Specific vesicular and target SNAREs operate in specific trafficking routes, but the degree of specificity of SNARE functions is still elus...

journal_title：Journal of cell science

authors： Pocard T,Le Bivic A,Galli T,Zurzolo C

更新日期：2007-09-15 00:00:00

abstract：:A cDNA encoding Xlamin B1 was cloned from a whole ovary mRNA by RT-PCR. GST-lamin fusion constructs were generated from this cDNA by first creating convenient restriction sites within the Xlamin B1 coding sequence, using PCR directed mutagenesis, and then sub-cloning relevant sequences into pGEX-4T-3. Two expression c...

journal_title：Journal of cell science

authors： Ellis DJ,Jenkins H,Whitfield WG,Hutchison CJ

更新日期：1997-10-01 00:00:00

abstract：:We have developed a procedure for sequential silver staining and in situ hybridization to analyze the relationship between the amount of rDNA present in nucleolar organizer regions, as estimated by in situ hybridization, and their level of expression, as estimated by the silver signal. For simplicity we used cells fro...

journal_title：Journal of cell science

authors： Zurita F,Jiménez R,Burgos M,de la Guardia RD

更新日期：1998-05-01 00:00:00

abstract：:Members of the PAR-1/MARK kinase family play critical roles in polarity and cell cycle control and are regulated by 14-3-3 scaffolding proteins, as well as the LKB1 tumour suppressor kinase and atypical protein kinase C (PKC). In this study, we initially investigated the mechanism underlying the interaction of mammali...

journal_title：Journal of cell science

authors： Göransson O,Deak M,Wullschleger S,Morrice NA,Prescott AR,Alessi DR

更新日期：2006-10-01 00:00:00

abstract：:Interstitial stem cells in Hydra are rapidly proliferating multipotent stem cells which continuously give rise to precursors for nerve and nematocyte differentiation. Growth of the stem cell population is controlled by the cell cycle time of the stem cells and the self-renewal probability, Ps (the fraction of stem cel...

journal_title：Journal of cell science

authors： Sproull F,David CN

更新日期：1979-08-01 00:00:00

abstract：:During anaphase-I in crane-fly spermatocytes, sister half-bivalents separate and move to opposite poles. When we irradiate a kinetochore spindle fibre with an ultraviolet microbeam, the associated half-bivalent temporarily stops moving and so does the partner half-bivalent with which it was paired during metaphase. To...

journal_title：Journal of cell science

authors： Yin B,Forer A

更新日期：1996-01-01 00:00:00

abstract：:Myogenic differentiation in adult muscle is normally suppressed and can be activated by myogenic cues in a subset of activated satellite cells. The switch mechanism that turns myogenesis on and off is not defined. In the present study, we demonstrate that tissue inhibitor of metalloproteinase 3 (TIMP3), the endogenous...

journal_title：Journal of cell science

authors： Liu H,Chen SE,Jin B,Carson JA,Niu A,Durham W,Lai JY,Li YP

更新日期：2010-09-01 00:00:00

abstract：:Podosomes are highly dynamic actin-based structures commonly found in motile and invasive cells such as macrophages, osteoclasts and vascular smooth muscle cells. Here, we have investigated the role of caldesmon, an actin-binding protein, in the formation of podosomes in aortic smooth muscle A7r5 cells induced by the ...

journal_title：Journal of cell science

authors： Eves R,Webb BA,Zhou S,Mak AS

更新日期：2006-05-01 00:00:00

abstract：:Notch signaling is a conserved signaling pathway implicated in embryogenesis and adult tissue maintenance. Notch signaling strength is strictly regulated, notably by maintaining a controlled pool of functional receptor at the cell surface. Mammalian non-activated Notch receptor is internalized, ubiquitylated by the It...

journal_title：Journal of cell science

authors： Puca L,Chastagner P,Meas-Yedid V,Israël A,Brou C

更新日期：2013-10-01 00:00:00

abstract：:Eukaryotic flagella undertake different beat types as necessary for different functions; for example, the Leishmania parasite flagellum undergoes a symmetric tip-to-base beat for forward swimming and an asymmetric base-to-tip beat to rotate the cell. In multi-ciliated tissues or organisms, the asymmetric beats are coo...

journal_title：Journal of cell science

authors： Wang Z,Beneke T,Gluenz E,Wheeler RJ

更新日期：2020-10-22 00:00:00

abstract：:Glutathione (GSH) levels progressively decline during aging and in neurodegenerative disorders. However, the contribution of such event in mediating neuronal cell death is still uncertain. In this report, we show that, in neuroblastoma cells as well as in primary mouse cortical neurons, GSH decrease, induced by buthio...

journal_title：Journal of cell science

authors： Aquilano K,Baldelli S,Cardaci S,Rotilio G,Ciriolo MR

更新日期：2011-04-01 00:00:00

abstract：:It is becoming clear that mechanical stimuli are crucial factors in regulating the biology of the cell, but the short-term structural response of a cell to mechanical forces remains relatively poorly understood. We mechanically stimulated cells transiently expressing actin-EGFP with controlled forces (0-20 nN) in orde...

journal_title：Journal of cell science

authors： Guolla L,Bertrand M,Haase K,Pelling AE

更新日期：2012-02-01 00:00:00

abstract：:Mechanical stimulation affects many biological aspects in living cells through mechanotransduction. In myogenic precursor cells (MPCs), mechanical stimulation activates p38 mitogen-activated protein kinase (MAPK), a key regulator of myogenesis, via activating TNFα-converting enzyme (TACE, also known as ADAM17), to rel...

journal_title：Journal of cell science

authors： Niu A,Wen Y,Liu H,Zhan M,Jin B,Li YP

更新日期：2013-10-01 00:00:00

abstract：:This study examined the role of Ca(2+) in regulatory volume decrease by Necturus erythrocytes. Hypotonic shock (50% tonicity) stimulated an increase in cytosolic free Ca(2+), detected using epi-fluorescence microscopy and the fluorescent Ca(2+) indicator fluo-4-AM (10 microM). A similar increase in fluorescence did no...

journal_title：Journal of cell science

authors： Light DB,Attwood AJ,Siegel C,Baumann NL

更新日期：2003-01-01 00:00:00

abstract：:Epidermal growth factor receptor (EGFR) ligands undergo a complex series of processing events during their maturation to active signaling proteins. Like its mammalian homologs, the predominant Drosophila EGFR ligand Spitz is produced as a transmembrane pro-protein. In the secretory pathway, Spitz is cleaved within its...

journal_title：Journal of cell science

authors： Steinhauer J,Liu HH,Miller E,Treisman JE

更新日期：2013-10-01 00:00:00

abstract：:The effect of a synthetic peptide consisting of Arg-Gly-Asp-Ser (RGDS), the amino acid sequence representing the fibroblast attachment site in fibronectin (FN), was tested on migrating newt epidermal cells. In one approach, skin explants were placed on the bottom of plastic dishes coated with human FN, human fibrinoge...

journal_title：Journal of cell science

authors： Donaldson DJ,Mahan JT,Smith GN Jr

更新日期：1987-05-01 00:00:00

abstract：:During spermatogenesis in mammals, the transcripts of transition protein 1 (TP 1) and protamine 1 (Prm 1) are under translational regulation. Following their transcription in round spermatids, the mRNAs for TP 1 and Prm 1 are stored in the cytoplasm from 3-7 days before being translated towards the end of spermatogene...

journal_title：Journal of cell science

authors： Morales CR,Kwon YK,Hecht NB

更新日期：1991-09-01 00:00:00

abstract：:Genomes exist in vivo as complex physical structures, and their functional output (i.e. the gene expression profile of a cell) is related to their spatial organization inside the nucleus as well as to local chromatin status. Chromatin modifications and chromosome conformation are distinct in different tissues and cell...

journal_title：Journal of cell science

authors： Naumova N,Dekker J

更新日期：2010-06-15 00:00:00

abstract：:Activator of G-protein signaling 3 (AGS3, also known as GPSM1) regulates the trans-Golgi network. The AGS3 GoLoco motif binds to Gαi and thereby regulates the transport of proteins to the plasma membrane. Compaction of early embryos is based on the accumulation of E-cadherin (Cdh1) at cell-contacted membranes. However...

journal_title：Journal of cell science

authors： Nie ZW,Niu YJ,Zhou W,Zhou DJ,Kim JY,Cui XS

更新日期：2020-12-07 00:00:00

abstract：:Certain embryonic tissue masses and cell aggregates behave like deformable solids during brief experimental manipulations but like viscous liquids in long-term organ cultures. To investigate these seemingly paradoxical physical properties, we have mechanically deformed cell aggregates derived from several embryonic ch...

journal_title：Journal of cell science

authors： Phillips HM,Steinberg MS

更新日期：1978-04-01 00:00:00

abstract：:It has been shown previously that whilst the rat type I integral membrane protein TGN38 (ratTGN38) is predominantly localised to the trans-Golgi network this protein does reach the cell surface from where it is internalised and delivered back to the trans-Golgi network. This protein thus provides a suitable tool for t...

journal_title：Journal of cell science

authors： Banting G,Maile R,Roquemore EP

更新日期：1998-12-01 00:00:00

abstract：:Rab GTPases regulate various membrane trafficking pathways but the mechanisms by which GTPase-activating proteins recognise specific Rabs are not clear. Rab8 is involved in controlling several trafficking processes, including the trafficking of transferrin receptor from the early endosome to the recycling endosome. He...

journal_title：Journal of cell science

authors： Vaibhava V,Nagabhushana A,Chalasani ML,Sudhakar C,Kumari A,Swarup G

更新日期：2012-11-01 00:00:00

abstract：:MACF1 (microtubule actin crosslinking factor), also called ACF7 (actin crosslinking family 7) is a cytoskeletal linker protein that can associate with both actin filaments and microtubules. We have identified a novel alternatively spliced isoform of MACF1. We named this isoform MACF1b and renamed the original isoform ...

journal_title：Journal of cell science

authors： Lin CM,Chen HJ,Leung CL,Parry DA,Liem RK

更新日期：2005-08-15 00:00:00

abstract：:Accurate chromosome segregation requires the spindle assembly checkpoint to be active at the onset of mitosis, before being silenced following chromosome alignment. p31(comet) is a checkpoint antagonist in that its inhibition delays mitotic exit, whereas its overexpression overrides the checkpoint. How exactly p31(com...

journal_title：Journal of cell science

authors： Westhorpe FG,Tighe A,Lara-Gonzalez P,Taylor SS

更新日期：2011-11-15 00:00:00

abstract：:Adult newt cardiomyocytes, in contrast to their mammalian counterparts, can proliferate after injury and contribute to the functional regeneration of the heart. In order to understand the mechanisms underlying this plasticity we performed longitudinal studies on single cardiomyocytes in culture. We find that the major...

journal_title：Journal of cell science

authors： Bettencourt-Dias M,Mittnacht S,Brockes JP

更新日期：2003-10-01 00:00:00

abstract：:The complex lipid constituents of the eukaryotic plasma membrane are precisely controlled in a cell-type-specific manner, suggesting an important, but as yet, unknown cellular function. Neuronal membranes are enriched in long-chain polyunsaturated fatty acids (LC-PUFAs) and alterations in LC-PUFA metabolism cause debi...

journal_title：Journal of cell science

authors： Lesa GM,Palfreyman M,Hall DH,Clandinin MT,Rudolph C,Jorgensen EM,Schiavo G

更新日期：2003-12-15 00:00:00