Chromosomes attain a metaphase position on half-spindles in the absence of an opposing spindle pole.

Abstract:

:To examine the relative roles of chromosomes, spindle poles and microtubules in the formation of the metaphase spindle and metakinesis, I have experimentally placed an extra centrosome-free pronucleus close to a forming bipolar spindle in a living cell. The chromosomes from the extra nucleus induce the formation of an extra half-spindle from one pole of the otherwise normal bipolar spindle with chromosomes positioned at the putative metaphase plate. I conclude that chromosomes determine the location of half-spindles by sustaining a higher than normal density of microtubules. These results are surprising for two reasons: first, because previous in vivo experiments in tissue culture cells show that mono-oriented chromosomes with functional attachments to spindle microtubules do not support half-spindle formation but oscillate unstably or move to one spindle pole. Additionally, the generally accepted view is that chromosomes attain a metastable condition at the metaphase plate as a result of a balance between forces directed to opposite spindle poles. However, our observation that chromosomes on extra half-spindles attain a metastable position in the absence of an opposing spindle pole, suggests that Ostergren's model does not account for metakinesis in sea urchin embryos.

journal_name

J Cell Sci

journal_title

Journal of cell science

authors

Leslie RJ

keywords:

subject

Has Abstract

pub_date

1992-09-01 00:00:00

pages

125-30

eissn

0021-9533

issn

1477-9137

journal_volume

103 ( Pt 1)

pub_type

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