Abstract：
:The tumor suppressor p53 is activated in response to many forms of cellular stress leading to cell cycle arrest, senescence or apoptosis. Appropriate sub-cellular localization is essential for modulating p53 function. We recently showed that p53 localizes to the nucleolus after proteasome inhibition with MG132 and this localization requires sequences within its carboxyl terminus. In the present study, we found that after treatment with MG132, p53 associates with a discrete sub-nucleolar component, the fibrillar center (FC), a region mainly enriched with RNA polymerase I. Moreover, we now demonstrate that this localization is an energy-dependent process as reduction of ATP levels prevents nucleolar localization. In addition, p53 sub-nucleolar accumulation is abolished when cells are subjected to various types of genotoxic stress. Furthermore, we show that monoubiquitination of p53, which causes it to localize to the cytoplasm and nucleoplasm, does not prevent the association of p53 with the nucleolus after MG132 treatment. Importantly, we demonstrate that p53 nucleolar association occurs in lung and bladder carcinomas.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Karni-Schmidt O,Zupnick A,Castillo M,Ahmed A,Matos T,Bouvet P,Cordon-Cardo C,Prives Cdoi
10.1242/jcs.030098subject
Has Abstractpub_date
2008-12-15 00:00:00pages
4098-105issue
Pt 24eissn
0021-9533issn
1477-9137pii
jcs.030098journal_volume
121pub_type
杂志文章abstract：:This study uses electron tomography linked to a variety of other EM methods to provide an integrated view of the flagellar pocket and basal body area of the African trypanosome procyclic trypomastigote. We reveal the pocket as an asymmetric membranous 'balloon' with two boundary structures. One of these - the collar -...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.045740
更新日期：2009-04-15 00:00:00
abstract：:We have previously shown that Xenopus oocytes, eggs, and early embryos contain lamins LII and LIII, and that portions of each are associated with distinct egg vesicle populations. We now report that a lamin similar or identical to the B-type lamin LI is also present in oocyte nuclei and in egg extracts. We have quanti...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-07-01 00:00:00
abstract：:Of the classical compartments of eukaryotic cells, peroxisomes were the last to be discovered. They are small, single-membrane-bound vesicles involved in cellular metabolism, most notably the beta-oxidation of fatty acids. Characterization of their properties and behavior has progressed rather slowly. However, during ...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.02893
更新日期：2006-03-15 00:00:00
abstract：:Low-intensity pulsed ultrasound (LIPUS) is a therapy used clinically to promote healing. Using live-cell imaging we show that LIPUS stimulation, acting through integrin-mediated cell-matrix adhesions, rapidly induces Rac1 activation associated with dramatic actin cytoskeleton rearrangements. Our study demonstrates tha...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.192781
更新日期：2017-07-15 00:00:00
abstract：:Neutrophils enter sites of inflammation by crossing the endothelial lining of the blood vessel wall. VE-cadherin is an endothelial specific, homophilic adhesion molecule located at the lateral cell surface. We have generated a monoclonal antibody against mouse VE-cadherin which inhibits electrical resistance of endoth...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1997-03-01 00:00:00
abstract：:Insect flight muscle has a large component (Tn-H) in the tropomyosin-troponin complex that is not present in vertebrate striated muscle thin filaments. Tn-H is shown by gold/Fab labelling to be present at regular intervals in insect flight muscle thin filaments. The Fab fragment of a monoclonal antibody to Tn-H was co...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
abstract：:Tau is a group of neuronal microtubule-associated proteins that are formed by alternative mRNA splicing and accumulate in neurofibrillary tangles in Alzheimer's disease (AD) brain. Tau plays a key role in regulating microtubule dynamics, axonal transport and neurite outgrowth, and all these functions of tau are modula...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.01558
更新日期：2004-11-15 00:00:00
abstract：:Rabbit muscle myosin subfragment S1 decorates 6 nm diameter filaments in Drosophila wing epidermal cells in the arrowhead fashion characteristic of the binding of subfragment S1 to actin filaments. The filaments in question are concentrated between microtubules that are mostly composed of 15 protofilaments and form ce...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1988-11-01 00:00:00
abstract：:Rab proteins comprise a family of monomeric GTPases that control cellular membrane traffic. Rab21 is a poorly characterised member with no known function. Human Rab21 cDNA from K562 cells was subcloned into GFP expression vectors to generate Rab21 and Rab21 mutants defective in either GTP hydrolysis (Rab21 Q78L) or bi...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01560
更新日期：2004-12-15 00:00:00
abstract：:In many physiological contexts, intracellular reduction-oxidation (redox) conditions and the unfolded protein response (UPR) are important for the control of cell life and death decisions. UPR is triggered by the disruption of endoplasmic reticulum (ER) homeostasis, also known as ER stress. Depending on the duration a...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.153643
更新日期：2014-09-01 00:00:00
abstract：:Excitatory amino acid transporter type 3 (EAAT3, also known as SLC1A1) is a high-affinity, Na(+)-dependent glutamate carrier that localizes primarily within the cell and at the apical plasma membrane. Although previous studies have reported proteins and sequence regions involved in EAAT3 trafficking, the detailed mole...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.185496
更新日期：2016-08-15 00:00:00
abstract：:Osteoclasts are giant multinucleated myeloid cells specialized for bone resorption, which is essential for the preservation of bone health throughout life. The activity of osteoclasts relies on the typical organization of osteoclast cytoskeleton components into a highly complex structure comprising actin, microtubules...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.244798
更新日期：2020-07-01 00:00:00
abstract：:Rac1 has a crucial role in epidermal growth factor (EGF)-induced membrane ruffling, lamellipodial protrusion, and cell migration. Several guanine nucleotide exchange factors (GEFs) including Sos1, Sos2, Tiam1 and Vav2 have been shown to transduce the growth signal from the EGF receptor to Rac1. To clarify the role of ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.028647
更新日期：2008-08-15 00:00:00
abstract：:CDC6, a replication licensing protein, is partially exported to the cytoplasm in human cells through phosphorylation by Cdk during S phase, but a significant proportion remains in the nucleus. We report here that human CDC6 physically interacts with ATR, a crucial checkpoint kinase, in a manner that is stimulated by p...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.058693
更新日期：2010-01-15 00:00:00
abstract：:Quantitative data are presented regarding cell proliferation and migration on (a) collagen films (b) the surface of 3-dimensional gels of native collagen fibres and (c) within the 3-dimensional collagen gel matrix, as part of a study of the effects of the extracellular matrix on cell behaviour. The nature of the colla...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1980-02-01 00:00:00
abstract：:Guinea pig sperm were suspended in calcium-containing medium supplemented with various concentrations of the tetrasaccharide, stachyose. At concentrations up to and including 0.6 M, stachyose was without effect on the A23187-induced acrosome reaction. At 1.0 M stachyose, greater than 97% of sperm retained their acroso...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:Mutations in the maternal-effect gene abnormal chromatin (abc) in Drosophila melanogaster result in a variety of defects involving nuclear replication/division. Three recessive alleles of this gene, which maps near 51F on chromosome 2, all result in female sterility. They cause slower embryonic development that is usu...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-02-01 00:00:00
abstract：:The conventional kinesin motor transports many different cargos to specific locations in neurons. How cargos regulate motor function remains unclear. Here we focus on KIF5, the heavy chain of conventional kinesin, and report that the Kv3 (Shaw) voltage-gated K(+) channel, the only known tetrameric KIF5-binding protein...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.122234
更新日期：2013-05-01 00:00:00
abstract：:Embryonic stem (ES) cells are cells derived from the early embryo that can be propagated indefinitely in the primitive undifferentiated state while remaining pluripotent; they share these properties with embryonic germ (EG) cells. Candidate ES and EG cell lines from the human blastocyst and embryonic gonad can differe...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：
更新日期：2000-01-01 00:00:00
abstract：:Prion diseases are fatal transmissible neurodegenerative disorders characterized by the accumulation of an abnormally folded isoform of the cellular prion protein (PrP(C)) denoted PrP(Sc). Recently, wild-type and pathogenic PrP mutants have been shown to be degraded by the endoplasmic reticulum-associated degradation ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01094
更新日期：2004-05-01 00:00:00
abstract：:Members of the PKC superfamily have been implicated in various migratory models and in particular in spatially restricted processes. However, defining the precise local events that underlie the PKC-dependent processes is constrained by the unspecific nature of interventions. Here we address this problem in relation to...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.062299
更新日期：2010-08-15 00:00:00
abstract：:The protein product of the gene responsible for ocular albinism type 1, named OA1, is a pigment-cell-specific membrane glycoprotein, displaying features of G-protein-coupled receptors, yet exclusively localized to late endosomes, lysosomes and melanosomes. To dissect the signals responsible for the intracellular local...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02930
更新日期：2006-05-15 00:00:00
abstract：:Two monoclonal antibodies, BA16 and BA17, have been developed using a detergent-insoluble extract of human mammary epithelial organoids as immunogen. Indirect immunofluorescent staining of cultured cells showed that the component reacting with the antibodies was filamentous and the intensity of staining was stronger i...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1985-04-01 00:00:00
abstract：:Human skin diseases have revealed fundamental mechanisms by which cytoskeletal proteins contribute to tissue architecture and function. In particular, the analysis of epidermal blistering disorders and the role of keratin gene mutations in these diseases has led to significant increases in our understanding of interme...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.02888
更新日期：2006-03-01 00:00:00
abstract：:Cytokines are supposed to be mediators in diarrhoeal diseases. The aim of this study is to characterize the effect of tumor necrosis factor-alpha (TNFalpha) on epithelial barrier function in the colonic epithelial cell line HT-29/B6. Active ion transport and barrier function were measured as short-circuit current and ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:A growing number of proteins originally found in endocytic structures of the plasma membrane appear to be able to traffic into the nucleus, but the cellular function of this translocation remains unclear. We have found that beta-arrestin2, which typically shows a cytoplasmic localization owing to constitutive nuclear ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03046
更新日期：2006-08-01 00:00:00
abstract：:It is now becoming clear that additional transmembrane components can modify integrin-mediated adhesion. Syndecan-4 is a transmembrane heparan sulfate proteoglycan whose external glycosaminoglycan chains can bind extracellular matrix ligands and whose core protein cytoplasmic domain can signal during adhesion. Two pap...
journal_title：Journal of cell science
pub_type： 评论,杂志文章,评审
doi：
更新日期：1999-10-01 00:00:00
abstract：:This study focuses on the effect of static and dynamic mechanical compression on the biosynthetic activity of chondrocytes cultured within agarose gel. Chondrocyte/agarose disks (3 mm diameter) were placed between impermeable platens and subjected to uniaxial unconfined compression at various times in culture (2-43 da...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
abstract：:The surface coat of procyclic forms of Trypanosoma brucei consists of related, internally repetitive glycoproteins known as EP and GPEET procyclins. Previously we showed that the extracellular domain of GPEET is phosphorylated. We now show that phosphorylation of this glycosylphosphatidylinositol-anchored surface prot...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-06-01 00:00:00
abstract：:In order to produce proteins essential for their propagation, many pathogenic human viruses, including SARS-CoV-2 the causative agent of COVID-19 respiratory disease, commandeer host biosynthetic machineries and mechanisms. Three major structural proteins, the spike, envelope and membrane proteins, are amongst several...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.257758
更新日期：2021-01-19 00:00:00
