A role for the small GTPase Rab21 in the early endocytic pathway.

Abstract:

:Rab proteins comprise a family of monomeric GTPases that control cellular membrane traffic. Rab21 is a poorly characterised member with no known function. Human Rab21 cDNA from K562 cells was subcloned into GFP expression vectors to generate Rab21 and Rab21 mutants defective in either GTP hydrolysis (Rab21 Q78L) or binding (Rab21 T33N) for transfection studies in HeLa cells. Confocal fluorescence microscopy and ultrastructural studies revealed Rab21 to be predominantly localised to the early endocytic pathway, on vesicles containing earlyendosomal antigen 1 EEA1, transferrin receptor and internalised ligands. EEA1 was localised to enlarged endosomes in Rab21 wild-type expressing cells but the GTP hydrolysis and GDP binding mutants had unique phenotypes labelling tubular reticular structures and the trans-Golgi network, respectively. Early endosome localisation for Rab21 was confirmed in a hepatoma cell line that allowed analysis of the subcellular distribution of the endogenous protein. Comparison of the localisation of Rab21 with other Rabs revealed extensive colocalisation with early endocytic variants Rab4, Rab5, Rab17 and Rab22 but much less overlap with those associated with late endosomes, recycling endosomes and the early secretory pathway. Cells expressing Rab21 T33N had defects in endocytosis of transferrin and epidermal growth factor and failed to effectively deliver the latter ligand to late endosomes and lysosomes for degradation. Collectively, our data provide the first characterisation of Rab21 function in early endosome dynamics.

journal_name

J Cell Sci

journal_title

Journal of cell science

authors

Simpson JC,Griffiths G,Wessling-Resnick M,Fransen JA,Bennett H,Jones AT

doi

10.1242/jcs.01560

keywords:

subject

Has Abstract

pub_date

2004-12-15 00:00:00

pages

6297-311

issue

Pt 26

eissn

0021-9533

issn

1477-9137

pii

jcs.01560

journal_volume

117

pub_type

杂志文章
  • Ability to organize microtubules in taxol-treated mitotic PtK2 cells goes with the SPN antigen and not with the centrosome.

    abstract::The SPN antigen plays an essential role in mitosis, since microinjection of antibodies causes mitotic arrest. Here we show, by examination of the relative locations of SPN antigen, the centrosomal 5051 antigen and tubulin in normal mitotic, and in taxol-treated mitotic cells, that the SPN antigen is involved in organi...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Kallajoki M,Weber K,Osborn M

    更新日期:1992-05-01 00:00:00

  • The dynamic properties of intermediate filaments during organelle transport.

    abstract::Intermediate filament (IF) dynamics during organelle transport and their role in organelle movement were studied using Xenopus laevis melanophores. In these cells, pigment granules (melanosomes) move along microtubules and microfilaments, toward and away from the cell periphery in response to alpha-melanocyte stimulat...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.046789

    authors: Chang L,Barlan K,Chou YH,Grin B,Lakonishok M,Serpinskaya AS,Shumaker DK,Herrmann H,Gelfand VI,Goldman RD

    更新日期:2009-08-15 00:00:00

  • Reactive oxygen species-mediated cytoplasmic stiffening impairs the phagocytic ability of the macrophage.

    abstract::The phagocytic ability of macrophages empowers them to enforce innate immunity. RAW264.7, THP-1 and peripheral blood mononuclear cell-derived macrophages display considerable variability with regards to their phagocytic ability. We identify the underlying causes that attenuate the phagocytic abilities of a macrophage....

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.236471

    authors: Agarwal M,Biswas P,Bhattacharya A,Sinha DK

    更新日期:2020-03-02 00:00:00

  • The desmosome: cell science lessons from human diseases.

    abstract::Human skin diseases have revealed fundamental mechanisms by which cytoskeletal proteins contribute to tissue architecture and function. In particular, the analysis of epidermal blistering disorders and the role of keratin gene mutations in these diseases has led to significant increases in our understanding of interme...

    journal_title:Journal of cell science

    pub_type: 杂志文章,评审

    doi:10.1242/jcs.02888

    authors: Kottke MD,Delva E,Kowalczyk AP

    更新日期:2006-03-01 00:00:00

  • Modulation of epidermal differentiation by epiligrin and integrin alpha 3 beta 1.

    abstract::We previously reported that integrin alpha 3 beta 1 mediates epidermal intercellular adhesion as well as cell-substrate adhesion. P1B5, an anti-alpha 3 beta 1 specific monoclonal antibody, is a potent in vitro trigger of epidermal cell-cell adhesion and an inhibitor of cell-substrate adhesion. We now show that P1B5 sp...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Symington BE,Carter WG

    更新日期:1995-02-01 00:00:00

  • Regulation of multiple target genes by miR-1 and miR-206 is pivotal for C2C12 myoblast differentiation.

    abstract::MicroRNAs are short non-coding RNAs involved in post-transcriptional regulation of multiple messenger RNA targets. The miR-1/miR-206 family is expressed during skeletal muscle differentiation and is an integral component of myogenesis. To better understand miR-1/miR-206 function during myoblast differentiation we iden...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.101758

    authors: Goljanek-Whysall K,Pais H,Rathjen T,Sweetman D,Dalmay T,Münsterberg A

    更新日期:2012-08-01 00:00:00

  • SH4-domain-induced plasma membrane dynamization promotes bleb-associated cell motility.

    abstract::SH4 domains provide bipartite membrane-targeting signals for oncogenic Src family kinases. Here we report the induction of non-apoptotic plasma membrane (PM) blebbing as a novel and conserved activity of SH4 domains derived from the prototypic Src kinases Src, Fyn, Yes and Lck as well as the HASPB protein of Leishmani...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.011130

    authors: Tournaviti S,Hannemann S,Terjung S,Kitzing TM,Stegmayer C,Ritzerfeld J,Walther P,Grosse R,Nickel W,Fackler OT

    更新日期:2007-11-01 00:00:00

  • A role for the dynamin-like protein Vps1 during endocytosis in yeast.

    abstract::Dynamins are a conserved family of proteins involved in membrane fusion and fission. Although mammalian dynamins are known to be involved in several membrane-trafficking events, the role of dynamin-1 in endocytosis is the best-characterised role of this protein family. Despite many similarities between endocytosis in ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.070508

    authors: Smaczynska-de Rooij II,Allwood EG,Aghamohammadzadeh S,Hettema EH,Goldberg MW,Ayscough KR

    更新日期:2010-10-15 00:00:00

  • The nebulin SH3 domain is dispensable for normal skeletal muscle structure but is required for effective active load bearing in mouse.

    abstract::Nemaline myopathy (NM) is a congenital myopathy with an estimated incidence of 150,000 live births. It is caused by mutations in thin filament components, including nebulin, which accounts for about 50% of the cases. The identification of NM cases with nonsense mutations resulting in loss of the extreme C-terminal SH3...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.137026

    authors: Yamamoto DL,Vitiello C,Zhang J,Gokhin DS,Castaldi A,Coulis G,Piaser F,Filomena MC,Eggenhuizen PJ,Kunderfranco P,Camerini S,Takano K,Endo T,Crescenzi M,Luther PK,Lieber RL,Chen J,Bang ML

    更新日期:2013-12-01 00:00:00

  • Galpha12 regulates protein interactions within the MDCK cell tight junction and inhibits tight-junction assembly.

    abstract::The polarized functions of epithelia require an intact tight junction (TJ) to restrict paracellular movement and to separate membrane proteins into specific domains. TJs contain scaffolding, integral membrane and signaling proteins, but the mechanisms that regulate TJs and their assembly are not well defined. Galpha12...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.014878

    authors: Sabath E,Negoro H,Beaudry S,Paniagua M,Angelow S,Shah J,Grammatikakis N,Yu AS,Denker BM

    更新日期:2008-03-15 00:00:00

  • cdc2 kinase-induced destabilization of MAP2-coated microtubules in Xenopus egg extracts.

    abstract::During the interphase to metaphase transition, microtubules are destabilized by a cdc2 kinase-dependent phosphorylation event. This destabilization is due to a dramatic increase in the rate at which each growing microtubule starts to shrink (catastrophe rate). In principle, this could be brought about by lowering the ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Faruki S,Dorée M,Karsenti E

    更新日期:1992-01-01 00:00:00

  • Ca2+ influx and protein scaffolding via TRPC3 sustain PKCbeta and ERK activation in B cells.

    abstract::Ca(2+) signaling mediated by phospholipase C that produces inositol 1,4,5-trisphosphate [Ins(1,4,5)P(3)] and diacylglycerol (DAG) controls lymphocyte activation. In contrast to store-operated Ca(2+) entry activated by Ins(1,4,5)P(3)-induced Ca(2+) release from endoplasmic reticulum, the importance of DAG-activated Ca(...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.061051

    authors: Numaga T,Nishida M,Kiyonaka S,Kato K,Katano M,Mori E,Kurosaki T,Inoue R,Hikida M,Putney JW Jr,Mori Y

    更新日期:2010-03-15 00:00:00

  • Localization of kinesin superfamily proteins to the connecting cilium of fish photoreceptors.

    abstract::Kinesin superfamily proteins (KIFs) are probable motors in vesicular and non-vesicular transport along microtubular tracks. Since a variety of KIFs have been recently identified in the motile flagella of Chlamydomonas, we sought to ascertain whether KIFs are also associated with the connecting cilia of vertebrate rod ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Beech PL,Pagh-Roehl K,Noda Y,Hirokawa N,Burnside B,Rosenbaum JL

    更新日期:1996-04-01 00:00:00

  • Drosophila Yemanuclein associates with the cohesin and synaptonemal complexes.

    abstract::Meiosis is characterized by two chromosome segregation rounds (meiosis I and II), which follow a single round of DNA replication, resulting in haploid genome formation. Chromosome reduction occurs at meiosis I. It relies on key structures, such as chiasmata, which are formed by repair of double-strand breaks (DSBs) be...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.152520

    authors: Meyer RE,Algazeery A,Capri M,Brazier H,Ferry C,Aït-Ahmed O

    更新日期:2014-11-01 00:00:00

  • Chromosome condensation activity in ovulated metaphase II mouse oocytes assayed by fusion with interphase blastomeres.

    abstract::Fusion of large and small karyoplasts produced from metaphase II mouse oocytes with interphase blastomeres from 2-cell and 8-cell embryos (volume ratio of partners, 1:1) results in premature chromosome condensation (PCC) of the interphase nucleus in the majority of the fusion products (hybrids). Fused under the same e...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Czołowska R,Waksmundzka M,Kubiak JZ,Tarkowski AK

    更新日期:1986-08-01 00:00:00

  • Dual capacity for nutrient uptake in Tetrahymena. V. Utilization of amino acids and proteins.

    abstract::We investigated the relative contributions of phagocytosis and plasma membrane transport to the uptake of amino acids and a protein (egg albumin) in amounts which allow Tetrahymena thermophila to grow and multiply. We used a mutant capable of indefinite growth without food vacuole formation (phagocytosis) and its wild...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Orias E,Rasmussen L

    更新日期:1979-04-01 00:00:00

  • Development of a macromolecular diffusion pathway in the lens.

    abstract::The mammalian lens consists of an aged core of quiescent cells enveloped by a layer of synthetically active cells. Abundant gap junctions within and between these cell populations ensure that the lens functions as an electrical syncytium and facilitates the exchange of small molecules between surface and core cells. I...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.00738

    authors: Shestopalov VI,Bassnett S

    更新日期:2003-10-15 00:00:00

  • Unconventional myosin VIIA promotes melanoma progression.

    abstract::Unconventional myosin VIIA (Myo7a) is an actin-based motor molecule that normally functions in the cochlear hair cells of the inner ear. Mutations of MYO7A/Myo7a have been implicated in inherited deafness in both humans and mice. However, there is limited information about the functions of Myo7a outside of the special...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.209924

    authors: Liu Y,Wei X,Guan L,Xu S,Yuan Y,Lv D,He X,Zhan J,Kong Y,Guo J,Zhang H

    更新日期:2018-02-22 00:00:00

  • Dynamic redistribution of calmodulin in HeLa cells during cell division as revealed by a GFP-calmodulin fusion protein technique.

    abstract::It has been suggested by many studies that Ca2+ signaling plays an important role in regulating key steps in cell division. In order to study the down stream components of calcium signaling, we have fused the gene of calmodulin (CaM) with that of green fluorescent protein (GFP) and expressed it in HeLa cells. The GFP-...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Li CJ,Heim R,Lu P,Pu Y,Tsien RY,Chang DC

    更新日期:1999-05-01 00:00:00

  • Cell surface glycoproteins change during gastrulation in Pleurodeles waltlii.

    abstract::Identification of the cell surface glycoproteins is an essential requirement for elucidating the mechanisms that mediate intercellular adhesion and cell migration in gastrulating amphibian embryos. In our experiments the glycoproteins present at the surface of isolated cells at blastula and gastrula stages were labell...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Riou JF,Darribère T,Boucaut JC

    更新日期:1986-06-01 00:00:00

  • Lipid- and protein-mediated multimerization of PSD-95: implications for receptor clustering and assembly of synaptic protein networks.

    abstract::Postsynaptic density protein 95 (PSD-95/SAP-90) is a palmitoylated membrane-associated guanylate kinase that oligomerizes and clusters ion channels and associated signaling machinery at excitatory synapses in brain. However, the mechanism for PSD-95 oligomerization and its relationship to ion channel clustering remain...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.00617

    authors: Christopherson KS,Sweeney NT,Craven SE,Kang R,El-Husseini Ael-D,Bredt DS

    更新日期:2003-08-01 00:00:00

  • Characterization of the ZO-1 protein in endothelial and other cell lines.

    abstract::A high molecular weight tight junction-associated protein, ZO-1, has been demonstrated in liver (hepatocytes) and in both epithelium and endothelium. We carried out studies to examine the presence of the protein in vascular endothelial cell cultures and several other types of cultured cells, and the relationship betwe...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Li CX,Poznansky MJ

    更新日期:1990-10-01 00:00:00

  • Heterogeneous proliferative potential in regenerative adult newt cardiomyocytes.

    abstract::Adult newt cardiomyocytes, in contrast to their mammalian counterparts, can proliferate after injury and contribute to the functional regeneration of the heart. In order to understand the mechanisms underlying this plasticity we performed longitudinal studies on single cardiomyocytes in culture. We find that the major...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.00698

    authors: Bettencourt-Dias M,Mittnacht S,Brockes JP

    更新日期:2003-10-01 00:00:00

  • Uncoupling proteasome peptidase and ATPase activities results in cytosolic release of an ER polytopic protein.

    abstract::The 26S proteasome is the primary protease responsible for degrading misfolded membrane proteins in the endoplasmic reticulum. Here we examine the specific role of beta subunit function on polypeptide cleavage and membrane release of CFTR, a prototypical ER-associated degradation substrate with 12 transmembrane segmen...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.02732

    authors: Oberdorf J,Carlson EJ,Skach WR

    更新日期:2006-01-15 00:00:00

  • Ancestral role of TNF-R pathway in cell differentiation in the basal metazoan Hydra.

    abstract::Tumour necrosis factor receptors (TNF-Rs) and their ligands, tumour necrosis factors, are highly conserved proteins described in all metazoan phyla. They function as inducers of extrinsic apoptotic signalling and facilitate inflammation, differentiation and cell survival. TNF-Rs use distinct adaptor molecules to activ...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.255422

    authors: Steichele M,Sauermann LS,König AC,Hauck S,Böttger A

    更新日期:2021-01-25 00:00:00

  • Regulation of bile canalicular network formation and maintenance by AMP-activated protein kinase and LKB1.

    abstract::AMP-activated protein kinase (AMPK), a cellular metabolic sensor, is essential in energy regulation and metabolism. Hepatocyte polarization during liver development and regeneration parallels increased metabolism. The current study investigates the effects of AMPK and its upstream activator LKB1 on polarity and bile c...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.068098

    authors: Fu D,Wakabayashi Y,Ido Y,Lippincott-Schwartz J,Arias IM

    更新日期:2010-10-01 00:00:00

  • TOM1 is a PI5P effector involved in the regulation of endosomal maturation.

    abstract::Phosphoinositides represent a major class of lipids specifically involved in the organization of signaling cascades, maintenance of the identity of organelles and regulation of multiple intracellular trafficking steps. We previously reported that phosphatidylinositol 5-monophosphate (PI5P), produced by the Shigella fl...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.166314

    authors: Boal F,Mansour R,Gayral M,Saland E,Chicanne G,Xuereb JM,Marcellin M,Burlet-Schiltz O,Sansonetti PJ,Payrastre B,Tronchère H

    更新日期:2015-02-15 00:00:00

  • N-Ras induces alterations in Golgi complex architecture and in constitutive protein transport.

    abstract::Aberrant glycosylation of proteins and lipids is a common feature of many tumor cell types, and is often accompanied by alterations in membrane traffic and an anomalous localization of Golgi-resident proteins and glycans. These observations suggest that the Golgi complex is a key organelle for at least some of the fun...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Babiá T,Ayala I,Valderrama F,Mato E,Bosch M,Santarén JF,Renau-Piqueras J,Kok JW,Thomson TM,Egea G

    更新日期:1999-02-01 00:00:00

  • Immunochemical and immunocytochemical identification of a myosin heavy chain polypeptide in Nicotiana pollen tubes.

    abstract::A myosin heavy chain polypeptide has been identified and localized in Nicotiana pollen tubes using monoclonal anti-myosin antibodies. The epitopes of these antibodies were found to reside on the myosin heavy chain head and rod portion and were, therefore, designated anti-S-1 (myosin S-1) and anti-LMM (light meromyosin...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:

    authors: Tang XJ,Hepler PK,Scordilis SP

    更新日期:1989-04-01 00:00:00

  • Rapid production of pure recombinant actin isoforms in Pichia pastoris.

    abstract::Actins are major eukaryotic cytoskeletal proteins, and they are involved in many important cell functions, including cell division, cell polarity, wound healing and muscle contraction. Despite obvious drawbacks, muscle actin, which is easily purified, is used extensively for biochemical studies of the non-muscle actin...

    journal_title:Journal of cell science

    pub_type: 杂志文章

    doi:10.1242/jcs.213827

    authors: Hatano T,Alioto S,Roscioli E,Palani S,Clarke ST,Kamnev A,Hernandez-Fernaud JR,Sivashanmugam L,Chapa-Y-Lazo B,Jones AME,Robinson RC,Sampath K,Mishima M,McAinsh AD,Goode BL,Balasubramanian MK

    更新日期:2018-04-23 00:00:00