Abstract:
:Members of the PKC superfamily have been implicated in various migratory models and in particular in spatially restricted processes. However, defining the precise local events that underlie the PKC-dependent processes is constrained by the unspecific nature of interventions. Here we address this problem in relation to atypical PKC (aPKC) action, which in conjunction with the exocyst complex controls the polarised delivery of promigratory signals. A drug-dependent recruitment approach was employed to manipulate the local recruitment of signals to the leading edge of migrating cells, under conditions where the aPKC-exocyst control is globally abrogated. We found that activation of ERK but not JNK at focal adhesions recovers the majority of the migratory loss attributed to ERK action, demonstrating a necessary role for active plasma membrane ERK in the downstream signalling of aPKC-dependent migration. The data further show that restored focal adhesion dynamics are a contributing mechanism through which localized ERK activity influences this aPKC-exocyst-dependent migration.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Boeckeler K,Rosse C,Howell M,Parker PJdoi
10.1242/jcs.062299subject
Has Abstractpub_date
2010-08-15 00:00:00pages
2725-32issue
Pt 16eissn
0021-9533issn
1477-9137pii
jcs.062299journal_volume
123pub_type
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