Cultured microvascular endothelial cells (MVEC) differ in cytoskeleton, expression of cadherins and fibronectin matrix. A study under the influence of interferon-gamma.

abstract：:The morphogenesis-related NDR kinase (MOR) pathway regulates morphogenesis in fungi. In spite of the high conservation of its components, impairing their functions results in highly divergent cellular responses depending on the fungal species. The reasons for such differences are unclear. Here we propose that the spec...

journal_title：Journal of cell science

authors： Sartorel E,Pérez-Martín J

更新日期：2012-10-01 00:00:00

abstract：:The mitochondrial influx and efflux of Ca(2+) play a relevant role in cytosolic and mitochondrial Ca(2+) homeostasis, and contribute to the regulation of mitochondrial functions in neurons. The mitochondrial Na(+)/Ca(2+) exchanger, which was first postulated in 1974, has been primarily investigated only from a functio...

journal_title：Journal of cell science

authors： Scorziello A,Savoia C,Sisalli MJ,Adornetto A,Secondo A,Boscia F,Esposito A,Polishchuk EV,Polishchuk RS,Molinaro P,Carlucci A,Lignitto L,Di Renzo G,Feliciello A,Annunziato L

更新日期：2013-12-15 00:00:00

abstract：:Receptor protein tyrosine phosphatase beta (RPTP beta) mediates cell-cell and cell-matrix interactions. By searching for intracellular proteins that interact with the cytoplasmic region of this phosphatase using the two-hybrid method, we identified several proteins containing PDZ domains. One of these proteins, MAGI-3...

journal_title：Journal of cell science

authors： Adamsky K,Arnold K,Sabanay H,Peles E

更新日期：2003-04-01 00:00:00

abstract：:The purpose of our studies was to determine the fate of different cell types present in early primary cultures of tracheal epithelial cells and, if possible, to elucidate the role they play in the establishment of the cultures. Epithelial cells were isolated from rat tracheas with 0.5% Pronase and were cultured on col...

journal_title：Journal of cell science

authors： Chang LY,Wu R,Nettesheim P

更新日期：1985-03-01 00:00:00

abstract：:Using epitope mapping we have demonstrated that a high molecular weight protein (Mr approximately 115 x 10(3)) present in brain and spinal cord is a member of the tau family of microtubule-associated proteins. Antibodies directed against the amino-terminal, middle and carboxyl-terminal portions of tau recognize this p...

journal_title：Journal of cell science

authors： Georgieff IS,Liem RK,Mellado W,Nunez J,Shelanski ML

更新日期：1991-09-01 00:00:00

abstract：:The human adenocarcinoma cell line Caco-2 was used for studies on the biosynthesis and transport of lysosomal acid alpha-glucosidase in polarized epithelial cells. Metabolic labelling revealed that in Caco-2 cells alpha-glucosidase is synthesized as a precursor form of 110 x 10(3) Mr. This form is converted into a pre...

journal_title：Journal of cell science

authors： Klumperman J,Fransen JA,Boekestijn TC,Oude Elferink RP,Matter K,Hauri HP,Tager JM,Ginsel LA

更新日期：1991-10-01 00:00:00

abstract：:The biosynthetic machinery in the melanotrope cells of the Xenopus intermediate pituitary is primarily dedicated to the generation of proopiomelanocortin (POMC)-derived, melanophore-stimulating peptides. Transfer of the animal to a black background stimulates the production of these peptides and causes a dramatic incr...

journal_title：Journal of cell science

authors： Holthuis JC,Jansen EJ,van Riel MC,Martens GJ

更新日期：1995-10-01 00:00:00

abstract：:Schizosaccharomyces pombe cells divide by medial fission throughout contraction of an actomyosin ring and deposition of a multilayered division septum that must be cleaved to release the two daughter cells. Although many studies have focused on the actomoysin ring and septum assembly, little information is available c...

journal_title：Journal of cell science

authors： Martín-Cuadrado AB,Dueñas E,Sipiczki M,Vázquez de Aldana CR,del Rey F

更新日期：2003-05-01 00:00:00

abstract：:Niemann-Pick disease type C (NPC) is an inherited lipid storage disorder caused by mutations in NPC1 or NPC2. NPC1 is a polytopic glycoprotein that contains a sterol-sensing domain, whereas NPC2 is a soluble protein that contains an MD-2-like lipid-recognition domain. In the current study, we addressed the hypothesis ...

journal_title：Journal of cell science

authors： Ohsaki Y,Sugimoto Y,Suzuki M,Hosokawa H,Yoshimori T,Davies JP,Ioannou YA,Vanier MT,Ohno K,Ninomiya H

更新日期：2006-07-01 00:00:00

abstract：:In the cytoplasm of chlorenchyma cells in primary Origanum dictamnus leaves, rectangular prism-like crystals occur, which, as shown by pepsin digestion, are proteinaceous. In sections they usually show either a square lattice or striations running parallel to the short or long axis. In both cases the interspacings are...

journal_title：Journal of cell science

authors： Bosabalidis AM,Papadopoulos D

更新日期：1983-11-01 00:00:00

abstract：:Serotonin receptors 5-HT(1A) and 5-HT(7) are highly coexpressed in brain regions implicated in depression. However, their functional interaction has not been established. In the present study we show that 5-HT(1A) and 5-HT(7) receptors form heterodimers both in vitro and in vivo. Foerster resonance energy transfer-bas...

journal_title：Journal of cell science

authors： Renner U,Zeug A,Woehler A,Niebert M,Dityatev A,Dityateva G,Gorinski N,Guseva D,Abdel-Galil D,Fröhlich M,Döring F,Wischmeyer E,Richter DW,Neher E,Ponimaskin EG

更新日期：2012-05-15 00:00:00

abstract：:In response to endovascular injury, platelet-derived growth factor-BB (PDGF-BB) and basic fibroblast growth factor (bFGF) are released locally and modulate vascular smooth muscle cells (SMC) proliferation and migration within the vascular wall. The aim of the present in vitro study was to determine how rat aorta SMC r...

journal_title：Journal of cell science

authors： Facchiano A,De Marchis F,Turchetti E,Facchiano F,Guglielmi M,Denaro A,Palumbo R,Scoccianti M,Capogrossi MC

更新日期：2000-08-01 00:00:00

abstract：:Mutations in a gene (Klp38B) encoding a novel kinesin-like protein in Drosophila melanogaster lead to the formation of polyploid cells in the larval central nervous system and in the follicle cells of adult egg chambers. Some homozygous mutants survive to adulthood and also exhibit morphological defects indicative of ...

journal_title：Journal of cell science

authors： Ohkura H,Török T,Tick G,Hoheisel J,Kiss I,Glover DM

更新日期：1997-04-01 00:00:00

abstract：:Protein with tau-like repeats (PTL-1) is the sole Caenorhabditis elegans homolog of tau and MAP2, which are members of the mammalian family of microtubule-associated proteins (MAPs). In mammalian neurons, tau and MAP2 are segregated, with tau being mainly localised to the axon and MAP2 mainly to the dendrite. In parti...

journal_title：Journal of cell science

authors： Chew YL,Fan X,Götz J,Nicholas HR

更新日期：2013-05-01 00:00:00

abstract：:Motile cilia generate directed hydrodynamic flow that is important for the motility of cells and extracellular fluids. To optimize directed hydrodynamic flow, motile cilia are organized and oriented into a polarized array. Basal bodies (BBs) nucleate and position motile cilia at the cell cortex. Cytoplasmic BB-associa...

journal_title：Journal of cell science

authors： Junker AD,Soh AWJ,O'Toole ET,Meehl JB,Guha M,Winey M,Honts JE,Gaertig J,Pearson CG

更新日期：2019-08-07 00:00:00

abstract：:The karyo-cytoskeleton of cells cultured in vitro was investigated employing resinless section immunogold electron microscopy. Cells were entrapped in low-melting agarose, sequentially extracted with various buffers and digested with nucleases to obtain karyo-cytoskeletal frameworks and reacted with specific primary a...

journal_title：Journal of cell science

authors： Wang X,Traub P

更新日期：1991-01-01 00:00:00

abstract：:The scaffold attachment factor SAFB1 and its recently discovered homologue SAFB2 might provide an important link between pre-mRNA splicing, intracellular signalling and transcription. Using novel mono-specific antisera, we found endogenous SAFB2 protein has a different spatial distribution from SAFB1 within the nucleu...

journal_title：Journal of cell science

authors： Sergeant KA,Bourgeois CF,Dalgliesh C,Venables JP,Stevenin J,Elliott DJ

更新日期：2007-01-15 00:00:00

abstract：:Tetrahymena cells secrete a factor which inhibits RNA synthesis in vivo and in vitro. The factor is a relatively small peptide with a molecular weight between 300 and 1500 Daltons. Other, non-specific peptides in the broth medium or added to a chemically defined medium have a stimulatory effect on RNA synthesis in viv...

journal_title：Journal of cell science

authors： Andersen HA,Lykkesfeldt AE,Nielsen SJ

更新日期：1980-10-01 00:00:00

abstract：:BMP-dependent patterning in the Drosophila melanogaster wing imaginal disc serves as a paradigm to understand how morphogens specify cell fates. The observed profile of the transcriptional response to the graded signal of BMP relies upon two counter-active gradients of pMad and Brinker (Brk). This patterning model is ...

journal_title：Journal of cell science

authors： Ziv O,Finkelstein R,Suissa Y,Dinur T,Deshpande G,Gerlitz O

更新日期：2012-12-01 00:00:00

abstract：:Nodal signaling induces the formation of the endoderm and mesoderm during gastrulation. Nodal expression persists until the definitive endoderm progenitor has completely formed, and disappears thereafter. A tightly regulated Nodal expression system is essential for the differentiation of embryonic stem (ES) cells into...

journal_title：Journal of cell science

authors： Takenaga M,Fukumoto M,Hori Y

更新日期：2007-06-15 00:00:00

abstract：:Cytoplasm of Paramecium caudatum syngen 3 in the period of sexual immaturity was effective in inducing immaturity when injected into mating-reactive cells of other syngens of P. caudatum. When immature cytoplasm of P. caudatum was injected into mature cells of P. multimicronucleatum, immaturity was also induced in the...

journal_title：Journal of cell science

authors： Miwa I

更新日期：1979-04-01 00:00:00

abstract：:Traction forces increase after microtubule depolymerization; however, the signaling mechanisms underlying this, in particular the dependence upon myosin II, remain unclear. We investigated the mechanism of traction force increase after nocodazole-induced microtubule depolymerization by applying traction force microsco...

journal_title：Journal of cell science

authors： Rape A,Guo WH,Wang YL

更新日期：2011-12-15 00:00:00

abstract：:The neuron specific Drosophila ELAV protein belongs to the ELAV family of RNA binding proteins which are characterized by three highly conserved RNA recognition motifs, an N-terminal domain, and a hinge region between the second and third RNA recognition motifs. Despite their highly conserved RNA recognition motifs th...

journal_title：Journal of cell science

authors： Yannoni YM,White K

更新日期：1999-12-01 00:00:00

abstract：:Madin-Darby canine kidney cells (MDCK) were transfected with a cDNA encoding the glycosyl-phosphatidylinositol (GPI)-anchored protein mouse Thy-1 in order to study the steady-state surface distribution of exogenous and endogenous GPI-linked proteins. Immunofluorescence of transfected cells grown on collagen-coated cov...

journal_title：Journal of cell science

authors： Wilson JM,Fasel N,Kraehenbuhl JP

更新日期：1990-05-01 00:00:00

abstract：:A correlation between intranuclear distribution of large tumor antigen (T-ag) and proliferative activity in Simian virus 40 (SV40)-transformed cells has been established. Nuclear T-ag was cytochemically detected by the immunoperoxidase reaction. Cell populations with a high growth rate displayed a heterogeneous patter...

journal_title：Journal of cell science

authors： Ordenes GE,Beck I,Carmona MT,Santos M

更新日期：1989-07-01 00:00:00

abstract：:The dynamics of cell adhesion sites control cell morphology and motility. Adhesion-site turnover is thought to depend on the local availability of the acidic phospholipid phosphatidylinositol-4,5-bisphosphate (PIP(2)). PIP(2) can bind to many cell adhesion proteins such as vinculin and talin, but the consequences of t...

journal_title：Journal of cell science

authors： Chandrasekar I,Stradal TE,Holt MR,Entschladen F,Jockusch BM,Ziegler WH

更新日期：2005-04-01 00:00:00

abstract：:CENP-C, one of the few known intrinsic proteins of the human centromere, is thought to play structural as well as regulatory roles crucial to proper chromosome segregation and mitotic progression. To further define the functions of CENP-C throughout the cell cycle we have used the yeast interaction trap to identify pr...

journal_title：Journal of cell science

authors： Pluta AF,Earnshaw WC,Goldberg IG

更新日期：1998-07-30 00:00:00

abstract：:Host/symbiont specificity has been investigated in non-symbiotic and aposymbiotic brown and green hydra infected with various free-living and symbiotic species and strains of Chlorella and Chlorococcum. Morphology and ultrastructure of the symbioses obtained have been compared. Aposymbiotic Swiss Hydra viridis and Jap...

journal_title：Journal of cell science

authors： Rahat M,Reich V

更新日期：1986-12-01 00:00:00

abstract：:Hormone secretion by pituitary cells is decreased by roscovitine, an inhibitor of cyclin-dependent kinase 5 (Cdk5). Roscovitine treatment reorganizes cortical actin and ultrastructural analysis demonstrates that roscovitine limits the ability of secretory granules to approach the plasma membrane or one another. Trio, ...

journal_title：Journal of cell science

authors： Xin X,Ferraro F,Bäck N,Eipper BA,Mains RE

更新日期：2004-09-15 00:00:00

abstract：:Cannabinoid receptors (CBRs) belong to the G protein-coupled receptor superfamily, and activation of CBRs in salivary cells inhibits agonist-stimulated salivation and modifies saliva content. However, the role of different CBR subtypes in acinar cell physiology and in intracellular signalling remains unclear. Here, we...

journal_title：Journal of cell science

authors： Kopach O,Vats J,Netsyk O,Voitenko N,Irving A,Fedirko N

更新日期：2012-04-15 00:00:00