PTL-1 regulates neuronal integrity and lifespan in C. elegans.

abstract：:The distribution of anionic sites on the membranes of rat pancreatic B cells and of their storage granules has been studied by the use of a visual probe of cationic ferritin. Membranes of isolated storage granules possessed a net negative charge which was apparently evenly distributed; the number of anionic sites was ...

journal_title：Journal of cell science

authors： Howell SL,Tyhurst M

更新日期：1977-01-01 00:00:00

abstract：:A method is reported for preparing surface (plasma) membranes from adrenal tumour (Y-1) cells. The procedure is based upon homogenization in hypotonic ZnCl2 followed by sedimentation through two sucrose density gradients. The purified membranes consist of large sheets of membrane. The identity and purity of the membra...

journal_title：Journal of cell science

authors： Osawa S,Hall PF

更新日期：1985-08-01 00:00:00

abstract：:The mitochondrial influx and efflux of Ca(2+) play a relevant role in cytosolic and mitochondrial Ca(2+) homeostasis, and contribute to the regulation of mitochondrial functions in neurons. The mitochondrial Na(+)/Ca(2+) exchanger, which was first postulated in 1974, has been primarily investigated only from a functio...

journal_title：Journal of cell science

authors： Scorziello A,Savoia C,Sisalli MJ,Adornetto A,Secondo A,Boscia F,Esposito A,Polishchuk EV,Polishchuk RS,Molinaro P,Carlucci A,Lignitto L,Di Renzo G,Feliciello A,Annunziato L

更新日期：2013-12-15 00:00:00

abstract：:We report the first direct analysis of the endogenous β-catenin phosphorylation activity in colon cancer SW480 cells. By comparing parental SW480 cells that harbor a typical truncated adenomatous polyposis coli (APC) form, cells expressing full-length APC and APC-depleted cells, we provide the formal demonstration tha...

journal_title：Journal of cell science

authors： Wang L,Liu X,Gusev E,Wang C,Fagotto F

更新日期：2014-04-15 00:00:00

abstract：:Intracellular pH has been measured by laser microspectrofluorimetry, using the pH-sensitive dyes SNARF-1, SNARF-calcein and SNARF-1-dextran. By this technique it was possible to accurately determine pH in volumes as small as 0.5 x 0.5 x 1 microns 3. The probes were loaded into the cells either by diffusion of their ac...

journal_title：Journal of cell science

authors： Seksek O,Bolard J

更新日期：1996-01-01 00:00:00

abstract：:The assembly of an organism requires the interaction between different layers of cells, in many cases via an extracellular matrix. In the developing Drosophila larva, muscles attach in an integrin-dependent manner to the epidermis, via a specialized extracellular matrix called tendon matrix. Tiggrin, a tendon matrix i...

journal_title：Journal of cell science

authors： Martin-Bermudo MD,Brown NH

更新日期：2000-11-01 00:00:00

abstract：:Currently >50 proteins have been reported to be associated with focal contacts and related ECM adhesions. Most of these contain multiple domains through which they can interact with different molecular partners, potentially forming a dense and heterogeneous protein network at the cytoplasmic faces of the adhesion site...

journal_title：Journal of cell science

authors： Zamir E,Geiger B

更新日期：2001-10-01 00:00:00

abstract：:Here we report Drosophila Waharan (Wah), a 170-kD predominantly nuclear protein with two potential human homologues, as a newly identified regulator of endosomal trafficking. Wah is required for neuromuscular-junction development and muscle integrity. In muscles, knockdown of Wah caused novel accumulations of tightly ...

journal_title：Journal of cell science

authors： Lone M,Kungl T,Koper A,Bottenberg W,Kammerer R,Klein M,Sweeney ST,Auburn RP,O'Kane CJ,Prokop A

更新日期：2010-07-15 00:00:00

abstract：:Cornichon proteins are structurally related transmembrane proteins that have been studied in and Drosophila and yeast. In Drosophila, Cornichon (Cni) is involved in embryo polarization by the TGFalpha-related Gurken. In yeast, the Cni-related Erv14 is required for axial budding. A cargo receptor function has been prop...

journal_title：Journal of cell science

authors： Castro CP,Piscopo D,Nakagawa T,Derynck R

更新日期：2007-07-15 00:00:00

abstract：:Borrelia burgdorferi is the causative agent of Lyme disease, a multisystemic disorder affecting primarily skin, joints and nervous system. Successful internalization and intracellular processing of borreliae by immune cells like macrophages is decisive for the outcome of a respective infection. Here, we use for the fi...

journal_title：Journal of cell science

authors： Klose M,Scheungrab M,Luckner M,Wanner G,Linder S

更新日期：2020-12-30 00:00:00

abstract：:To examine the relative roles of chromosomes, spindle poles and microtubules in the formation of the metaphase spindle and metakinesis, I have experimentally placed an extra centrosome-free pronucleus close to a forming bipolar spindle in a living cell. The chromosomes from the extra nucleus induce the formation of an...

journal_title：Journal of cell science

authors： Leslie RJ

更新日期：1992-09-01 00:00:00

abstract：:Transcription factor IIH (TFIIH) participates in transcription, nucleotide excision repair and the control of the cell cycle. In the present study, we demonstrate that the Dmp52 subunit of TFIIH in Drosophila physically interacts with the fly p53 homologue, Dp53. The depletion of Dmp52 in the wing disc generates chrom...

journal_title：Journal of cell science

authors： Villicaña C,Cruz G,Zurita M

更新日期：2013-06-01 00:00:00

abstract：:Receptor protein tyrosine phosphatase beta (RPTP beta) mediates cell-cell and cell-matrix interactions. By searching for intracellular proteins that interact with the cytoplasmic region of this phosphatase using the two-hybrid method, we identified several proteins containing PDZ domains. One of these proteins, MAGI-3...

journal_title：Journal of cell science

authors： Adamsky K,Arnold K,Sabanay H,Peles E

更新日期：2003-04-01 00:00:00

abstract：:We have identified a novel autoantibody specificity in scleroderma that we term anti-chromo. These antibodies recognize several chromosomal antigens with apparent molecular mass of between 23 and 25 kDa, as determined by immunoblots. Anti-chromo autoantibodies occur in 10-15% of sera from patients with anti-centromere...

journal_title：Journal of cell science

authors： Saunders WS,Chue C,Goebl M,Craig C,Clark RF,Powers JA,Eissenberg JC,Elgin SC,Rothfield NF,Earnshaw WC

更新日期：1993-02-01 00:00:00

abstract：:T cell activation is accompanied by actin-mediated receptor clustering and reorganization of lipid rafts. It has been suggested that costimulatory molecules might be involved in these processes. We here provide evidence that engagement of the adhesion molecule CD44 initiates cytoskeletal rearrangement and membrane reo...

journal_title：Journal of cell science

authors： Föger N,Marhaba R,Zöller M

更新日期：2001-03-01 00:00:00

abstract：:Atherosclerosis depends on risk factors such as hyperlipidemia, smoking, hypertension and diabetes. Although these risk factors are relatively constant throughout the arterial circulation, atherosclerotic plaques occur at specific sites where flow patterns are disturbed, with lower overall magnitude and complex change...

journal_title：Journal of cell science

authors： Conway DE,Schwartz MA

更新日期：2013-11-15 00:00:00

abstract：:Several properties of cadherin-4 and cadherin-5 were characterized by using the cDNA transfection approach. The proteins of both cadherins had a relative molecular mass of about 130 kDa and were present at the cell periphery, especially at cell-cell contact sites. These cadherins were easily digested with trypsin, and...

journal_title：Journal of cell science

authors： Tanihara H,Kido M,Obata S,Heimark RL,Davidson M,St John T,Suzuki S

更新日期：1994-06-01 00:00:00

abstract：:Rap1 is a Ras-like small GTPase that is activated by many extracellular stimuli and strongly implicated in the control of integrin-mediated cell adhesion. Recent evidence indicates that Rap1 also plays a key role in formation of cadherin-based cell-cell junctions. Indeed, inhibition of Rap1 generates immature adherens...

journal_title：Journal of cell science

authors： Kooistra MR,Dubé N,Bos JL

更新日期：2007-01-01 00:00:00

abstract：:Endoblast and hypoblast tissue, dissected from early chick embryos, was explanted and cultured on glass or plastic substrata. These tissues grew rapidly to form epithelial sheets. Under the same conditions, mesoderm, dissected without the aid of dissociating agents, grew poorly. After 24 h in culture, the mesoderm exp...

journal_title：Journal of cell science

authors： Sanders EJ

更新日期：1980-08-01 00:00:00

abstract：:The Wnt signal transduction pathway regulates various aspects of embryonal development and is involved in cancer formation. Wnts induce the stabilisation of cytosolic (beta)-catenin, which then associates with TCF transcription factors to regulate expression of Wnt-target genes. At various levels the Wnt pathway is su...

journal_title：Journal of cell science

authors： Behrens J

更新日期：2000-03-01 00:00:00

abstract：:Patients with myotonic dystrophy type 1 exhibit a diversity of symptoms that affect many different organs. Among these are cognitive dysfunctions, the origin of which has remained elusive, partly because of the difficulty in accessing neural cells. Here, we have taken advantage of pluripotent stem cell lines derived f...

journal_title：Journal of cell science

authors： Denis JA,Gauthier M,Rachdi L,Aubert S,Giraud-Triboult K,Poydenot P,Benchoua A,Champon B,Maury Y,Baldeschi C,Scharfmann R,Piétu G,Peschanski M,Martinat C

更新日期：2013-04-15 00:00:00

abstract：:During vertebrate gastrulation, a well-orchestrated series of morphogenetic changes leads to the formation of the three germ layers: the ectoderm, mesoderm and endoderm. The analysis of gene expression patterns during gastrulation has been central to the identification of genes involved in germ layer formation. Howeve...

journal_title：Journal of cell science

authors： Link V,Carvalho L,Castanon I,Stockinger P,Shevchenko A,Heisenberg CP

更新日期：2006-05-15 00:00:00

abstract：:We have applied fluorescence ratio imaging to the analysis of an actin-binding protein concentration relative to F-actin in macrophages, in order to explore the role of a novel (alpha)-actinin isoform, actinin-4, relative to that of the classical isoform, actinin-1. Conventional immunofluorescence images showed that b...

journal_title：Journal of cell science

authors： Araki N,Hatae T,Yamada T,Hirohashi S

更新日期：2000-09-01 00:00:00

abstract：:Formins constitute a family of eukaryotic proteins that are considered to function as a cytoskeleton organizer to regulate morphogenesis, cell polarity and cytokinesis. Fhos is a recently identified mammalian formin, which contains the conserved domains FH (formin homology) 1 and FH2 in the middle region and the Dia-a...

journal_title：Journal of cell science

authors： Takeya R,Sumimoto H

更新日期：2003-11-15 00:00:00

abstract：:The presence of high-ploidy cells in malignant tumours has long been documented. However, the biological significance of these cells is not known and there is a great deal of controversy over their proliferative potential. We have analysed the behaviour of these cells in two murine tumour lines, B16F10 melanoma and 3T...

journal_title：Journal of cell science

authors： de la Hoz C,Baroja A

更新日期：1993-01-01 00:00:00

abstract：:The family of integrin transmembrane receptors is essential for the normal function of multicellular organisms by facilitating cell-extracellular matrix adhesion. The vitronectin-binding integrin αVβ5 localizes to focal adhesions (FAs) as well as poorly characterized flat clathrin lattices (FCLs). Here, we show that, ...

journal_title：Journal of cell science

authors： Zuidema A,Wang W,Kreft M,Te Molder L,Hoekman L,Bleijerveld OB,Nahidiazar L,Janssen H,Sonnenberg A

更新日期：2018-11-05 00:00:00

abstract：:Mitosis involves considerable membrane remodelling and vesicular trafficking to generate two independent cells. Consequently, endocytosis and endocytic proteins are required for efficient mitotic progression and completion. Several endocytic proteins also participate in mitosis in an endocytosis-independent manner. He...

journal_title：Journal of cell science

authors： Ma MP,Chircop M

更新日期：2012-09-15 00:00:00

abstract：:We have performed a screening analysis of differential gene expression using a defined in vitro model of human capillary tube formation. Gene array, differential display and cDNA library screening were used to identify both known and novel differentially expressed genes. Major findings include: the upregulation and fu...

journal_title：Journal of cell science

authors： Bell SE,Mavila A,Salazar R,Bayless KJ,Kanagala S,Maxwell SA,Davis GE

更新日期：2001-08-01 00:00:00

abstract：:Expression of actin was examined in a cultured rat embryonic cell line 3Y1 and transformed cell lines that originated from 3Y1. An alpha-actin in addition to cytoplasmic beta- and gamma-actins was detected in 3Y1 by two-dimensional gel electrophoresis. This alpha-actin was hardly detected at all in the transformants i...

journal_title：Journal of cell science

authors： Okamoto-Inoue M,Taniguchi S,Sadano H,Kawano T,Kimura G,Gabbiani G,Baba T

更新日期：1990-08-01 00:00:00

abstract：:Alix is a cytosolic protein in mammalian cells that was originally identified on the basis of its association with pro-apoptotic signaling. More recent evidence has established that Alix has a hand in regulating other cellular mechanisms, including endocytic membrane trafficking and cell adhesion. Although Alix appear...

journal_title：Journal of cell science

authors： Odorizzi G

更新日期：2006-08-01 00:00:00