Abstract：
:The postnatal mouse ovary is rich in quiescent and early-growing oocytes, each one surrounded by a layer of somatic granulosa cells (GCs) on a basal lamina. As oocytes start to grow the GCs change shape from flattened to cuboidal, increase their proliferation and form multiple layers, providing a unique model for studying the relationship between cell shape, proliferation and multilayering within the context of two different intercommunicating cell types: somatic and germ cells. Proliferation of GCs was quantified using immunohistochemistry for Ki67 and demonstrated that, unusually, cuboidal cells divided more than flat cells. As a second layer of GCs started to appear, cells on the basal lamina reached maximum packing density and the axes of their mitoses became perpendicular to the basal lamina, resulting in cells dividing inwards to form second and subsequent layers. Proliferation of basal GCs was less than that of inner cells. Ultrastructurally, collagen fibrils outside the basal lamina became more numerous as follicles developed. We propose that the basement membrane and/or theca cells that surround the follicle provide an important confinement for rapidly dividing columnar cells so that they attain maximum packing density, which restricts lateral mitosis and promotes inwardly oriented cell divisions and subsequent multilayering.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Da Silva-Buttkus P,Jayasooriya GS,Mora JM,Mobberley M,Ryder TA,Baithun M,Stark J,Franks S,Hardy Kdoi
10.1242/jcs.036400subject
Has Abstractpub_date
2008-12-01 00:00:00pages
3890-900issue
Pt 23eissn
0021-9533issn
1477-9137pii
jcs.036400journal_volume
121pub_type
杂志文章abstract：:The myosin regulatory light chain (RLC) of Dictyostelium discoideum is phosphorylated at a single serine site in response to chemoattractant. To investigate the role of the phosphorylation of RLC in both motility and chemotaxis, mutants were generated in which the single phosphorylatable serine was replaced with a non...
journal_title：Journal of cell science
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更新日期：2002-04-15 00:00:00
abstract：:Thrombospondin-1 (TSP-1) and the highly related protein thrombospondin-2 (TSP-2) are trimeric extracellular molecules that have complex roles in wound healing, angiogenesis and matrix organisation. At the cellular level, TSP-1 supports cell adhesion and migration by the organisation of fascin spike cytoskeletal struct...
journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2002-06-01 00:00:00
abstract：:Several cell motility processes including cytokinesis and cell locomotion are dependent on the interplay of the microtubule and actomyosin cytoskeletons. However, because such processes are essentially visual phenomena, interactions between the two cytoskeletal systems have been difficult to study quantitatively. To o...
journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：1997-08-01 00:00:00
abstract：:Disruption of the sarcoglycan complex leads to muscle membrane instability and muscular dystrophy in humans and mice. Through the dystrophin glycoprotein complex, sarcoglycan participates in connecting the internal cytoskeleton to the membrane and the extracellular matrix. Integrin alpha7beta1 is also a transmembrane ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01234
更新日期：2004-08-01 00:00:00
abstract：:Previous studies have shown that the non-alpha-helical, amino-terminal head region of vimentin is essential for the formation and stability of vimentin intermediate filaments (IFs). In order to specify its target site on companion protein subunits, it was cut off from vimentin at amino acid position 96 with lysine-spe...
journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：1992-02-01 00:00:00
abstract：:The essential function of the T cell receptor (TCR) is to translate the engagement of peptides on the major histocompatibility complex (pMHC) into appropriate intracellular signals through the associated cluster of differentiation 3 (CD3) complex. The spatial organization of the TCR-CD3 complex in the membrane is thou...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
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更新日期：2019-02-11 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-02-15 00:00:00
abstract：:Ligand-dependent transcriptional regulation by nuclear receptors is believed to be mediated by intermediary factors (TIFs) acting on remodelling of the chromatin structure and/or the activity of the transcriptional machinery. The putative transcriptional mediator TIF1alpha is a nuclear protein kinase that has been ide...
journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：1999-06-01 00:00:00
abstract：:gamma-Tubulin exists in fission yeast as the product of an essential gene, encoding a 446 amino acid protein that is 77.3% identical to Aspergillus nidulans gamma-tubulin. The gene disruption caused cell lethality, displaying condensed, undivided chromosomes with aberrant spindle structures. Anti-gamma-tubulin stainin...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-08-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章,评审
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journal_title：Journal of cell science
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更新日期：2011-12-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1977-08-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1979-02-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.004200
更新日期：2007-07-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01302
更新日期：2004-08-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1980-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.125757
更新日期：2013-08-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1985-10-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1986-02-01 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-08-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2000-02-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-05-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2015-11-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-05-01 00:00:00
abstract：:We have found that Ehrlich ascites tumour (EAT) cells, deprived of any carbon source, and suspended at a density of 2 X 10(5) cells/cm3, begin to die only after 12 h of starvation, though it is known that under these conditions they lose over 80% of their ATP within 30 min. Moreover, we have found that the viability o...
journal_title：Journal of cell science
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更新日期：1985-08-01 00:00:00
