Novel function of beta-arrestin2 in the nucleus of mature spermatozoa.

abstract：:Annexin A2 (AnxA2) is a multi-functional and -compartmental protein whose subcellular localisation and functions are tightly regulated by its post-translational modifications. AnxA2 and its Tyr23-phosphorylated form (pTyr23AnxA2) are involved in malignant cell transformation, metastasis and angiogenesis. Here, we show...

journal_title：Journal of cell science

authors： Grindheim AK,Hollås H,Raddum AM,Saraste J,Vedeler A

更新日期：2016-01-15 00:00:00

abstract：:MicroRNAs are known to play regulatory roles in gene expression associated with cancer development. We analyzed levels of the microRNA miR-24 in patients with breast carcinoma and found that miR-24 was higher in breast carcinoma samples than in benign breast tissues. We generated constructs expressing miR-24 and studi...

journal_title：Journal of cell science

authors： Du WW,Fang L,Li M,Yang X,Liang Y,Peng C,Qian W,O'Malley YQ,Askeland RW,Sugg SL,Qian J,Lin J,Jiang Z,Yee AJ,Sefton M,Deng Z,Shan SW,Wang CH,Yang BB

更新日期：2013-03-15 00:00:00

abstract：:We reported previously that Ca2+ depletion of Dictyostelium discoideum cells severely inhibits extracellular cyclic nucleotide phosphodiesterase (PD) synthesis at a post-transcriptional step. In this study, further experiments were performed to learn more about the nature of this phenomenon. Examination of the polysom...

journal_title：Journal of cell science

authors： Coukell MB,Cameron AM,Adames NR

更新日期：1992-10-01 00:00:00

abstract：:Maintenance of epithelial polarity depends on the correct localization and levels of polarity determinants. The evolutionarily conserved transmembrane protein Crumbs is crucial for the size and identity of the apical membrane, yet little is known about the molecular mechanisms controlling the amount of Crumbs at the s...

journal_title：Journal of cell science

authors： Lin YH,Currinn H,Pocha SM,Rothnie A,Wassmer T,Knust E

更新日期：2015-12-15 00:00:00

abstract：:We report here an analysis of the execution point of the temperature-sensitive Saccharomyces cerevisiae cell cycle mutant, cdc27-47. When a logarithmically growing culture was shifted from standard growth conditions (strain 27.8B growing in YEPD at 25 degrees C) to the restrictive temperature cell division ceased abru...

journal_title：Journal of cell science

authors： Richmond KM,Williamson DH

更新日期：1983-11-01 00:00:00

abstract：:The biosynthetic machinery in the melanotrope cells of the Xenopus intermediate pituitary is primarily dedicated to the generation of proopiomelanocortin (POMC)-derived, melanophore-stimulating peptides. Transfer of the animal to a black background stimulates the production of these peptides and causes a dramatic incr...

journal_title：Journal of cell science

authors： Holthuis JC,Jansen EJ,van Riel MC,Martens GJ

更新日期：1995-10-01 00:00:00

abstract：:The phagocytic ability of macrophages empowers them to enforce innate immunity. RAW264.7, THP-1 and peripheral blood mononuclear cell-derived macrophages display considerable variability with regards to their phagocytic ability. We identify the underlying causes that attenuate the phagocytic abilities of a macrophage....

journal_title：Journal of cell science

authors： Agarwal M,Biswas P,Bhattacharya A,Sinha DK

更新日期：2020-03-02 00:00:00

abstract：:Assembly of contractile apparatuses in striated muscle requires precisely regulated reorganization of the actin cytoskeletal proteins into sarcomeric organization. Regulation of actin filament dynamics is one of the essential processes of myofibril assembly, but the mechanism of actin regulation in striated muscle is ...

journal_title：Journal of cell science

authors： Nomura K,Ono K,Ono S

更新日期：2012-09-01 00:00:00

abstract：:Mammalian homologs of the Drosophila TRP protein have been shown to form cation-permeable channels in the plasma membrane but very little is known about the mechanisms that control their cell surface localization. Recently it has been demonstrated that the last three C-terminal amino acids (TRL) of TRPC4 comprise a PD...

journal_title：Journal of cell science

authors： Mery L,Strauss B,Dufour JF,Krause KH,Hoth M

更新日期：2002-09-01 00:00:00

abstract：:The proteasome is the major degradation machinery of the cell that regulates multiple cellular processes as diverse as cell cycle, signal transduction and gene expression. Recognition and unfolding of target proteins involves the regulatory cap whose base contains six AAA-ATPases that display reverse chaperone activit...

journal_title：Journal of cell science

authors： Müller D,Nagel AC,Maier D,Preiss A

更新日期：2006-01-15 00:00:00

abstract：:The translationally controlled protein P23 was discovered by the early induction of its rate of synthesis after mitogenic stimulation of mouse fibroblasts. P23 is expressed in almost all mammalian tissues and it is highly conserved between animals, plants and yeast. Based on its amino acid sequence, P23 cannot be attr...

journal_title：Journal of cell science

authors： Gachet Y,Tournier S,Lee M,Lazaris-Karatzas A,Poulton T,Bommer UA

更新日期：1999-04-01 00:00:00

abstract：:The insulin receptor tyrosine kinase substrate p53 (IRSp53) links Rac and WAVE2 and has been implicated in lamellipodia protrusion. Recently, however, IRSp53 has been reported to bind to both Cdc42 and Mena to induce filopodia. To shed independent light on IRSp53 function we determined the localisations and dynamics o...

journal_title：Journal of cell science

authors： Nakagawa H,Miki H,Nozumi M,Takenawa T,Miyamoto S,Wehland J,Small JV

更新日期：2003-06-15 00:00:00

abstract：:Phosphorylation regulates assembly and disassembly of proteins during endocytosis. In yeast, Prk1 and Ark1 phosphorylate factors after vesicle internalization leading to coat disassembly. Scd5, a protein phosphatase-1 (PP1)-targeting subunit, is proposed to regulate dephosphorylation of Prk1/Ark1 substrates to promote...

journal_title：Journal of cell science

authors： Chi RJ,Torres OT,Segarra VA,Lansley T,Chang JS,Newpher TM,Lemmon SK

更新日期：2012-10-15 00:00:00

abstract：:Linear chains of five to hundreds of phosphates called polyphosphate are found in organisms ranging from bacteria to humans, but their function is poorly understood. In Dictyostelium discoideum, polyphosphate is used as a secreted signal that inhibits cytokinesis in an autocrine negative feedback loop. To elucidate ho...

journal_title：Journal of cell science

authors： Suess PM,Watson J,Chen W,Gomer RH

更新日期：2017-07-15 00:00:00

abstract：:Semaphorin 3A (Sema3A), a secretory semaphorin, exerts various biological actions through a complex between neuropilin-1 and plexin-As (PlexAs). Sema3A induces retrograde signaling, which is involved in regulating dendritic localization of GluA2 (also known as GRIA2), an AMPA receptor subunit. Here, we investigated a ...

journal_title：Journal of cell science

authors： Yamashita N,Yamane M,Suto F,Goshima Y

更新日期：2016-05-01 00:00:00

abstract：:Circadian rhythms exist in most living organisms. The general molecular mechanisms that are used to generate 24-hour rhythms are conserved among organisms, although the details vary. These core clocks consist of multiple regulatory feedback loops, and must be coordinated and orchestrated appropriately for the fine-tun...

journal_title：Journal of cell science

authors： Kojima S,Shingle DL,Green CB

更新日期：2011-02-01 00:00:00

abstract：:Tetrahymena thermophila swims by the coordinated beating of hundreds of cilia that cover its body. It has been proposed that the outer arm dyneins of the ciliary axoneme control beat frequency, whereas the inner arm dyneins control waveform. To test the role of one of these inner arms, dynein heavy chain 7 protein (Dy...

journal_title：Journal of cell science

authors： Wood CR,Hard R,Hennessey TM

更新日期：2007-09-01 00:00:00

abstract：:Polarity of many cell types is controlled by a protein complex consisting of Bazooka/PAR-3 (Baz), PAR-6 and atypical protein kinase C (aPKC). In Drosophila, the Baz-PAR-6-aPKC complex is required for the control of cell polarity in the follicular epithelium, in ectodermal epithelia and neuroblasts. aPKC is the main si...

journal_title：Journal of cell science

authors： Kim S,Gailite I,Moussian B,Luschnig S,Goette M,Fricke K,Honemann-Capito M,Grubmüller H,Wodarz A

更新日期：2009-10-15 00:00:00

abstract：:Previous reports have suggested that Cx26 exhibits unique intracellular transport pathways en route to the cell surface compared with other members of the connexin family. To directly examine and compare nascent and steady-state delivery of Cx43 and Cx26 to the plasma membrane and gap junction biogenesis we expressed ...

journal_title：Journal of cell science

authors： Thomas T,Jordan K,Simek J,Shao Q,Jedeszko C,Walton P,Laird DW

更新日期：2005-10-01 00:00:00

abstract：:MicroRNAs (miRNAs) play important roles in the development of skeletal muscle. In our previous study, expression of miR-195 and miR-497 were shown to be upregulated during muscle development in pigs. In this study, we investigated the roles of these two miRNAs in myogenesis and analyzed their transcriptional regulatio...

journal_title：Journal of cell science

authors： Wei W,Zhang WY,Bai JB,Zhang HX,Zhao YY,Li XY,Zhao SH

更新日期：2016-01-01 00:00:00

abstract：:Clusterin expression is increased in tissues undergoing apoptosis, including neurodegenerative retina, but the causal relationships remain to be clarified. To test the hypothesis that overexpression of clusterin could induce apoptosis in neurons, transgenic mice were generated in which rat clusterin transgene was expr...

journal_title：Journal of cell science

authors： Jomary C,Chatelain G,Michel D,Weston A,Neal MJ,Jones SE

更新日期：1999-05-01 00:00:00

abstract：:Peritubular cells from 15- and 25-day-old rat testis trapped in collagen lattices caused those lattices to contract. Contraction proceeded more rapidly and to a greater extent using cells from younger rats. When 36,000 cells from 15- and 25-day-old rats were trapped in 800 mm2 lattices, the areas were reduced to 28 mm...

journal_title：Journal of cell science

authors： Ehrlich HP,Borland KM,Muffly KE,Hall PF

更新日期：1986-06-01 00:00:00

abstract：:Several studies have shown that altered gravity conditions influence mammalian cell growth and differentiation. The molecular mechanisms underlying these effects, however, remain relatively obscure. In this paper we show that microgravity reached in a sounding rocket strongly decreases epidermal growth factor (EGF)-in...

journal_title：Journal of cell science

authors： de Groot RP,Rijken PJ,den Hertog J,Boonstra J,Verkleij AJ,de Laat SW,Kruijer W

更新日期：1990-09-01 00:00:00

abstract：:MACF (microtubule actin cross-linking factor) is a large, 608-kDa protein that can associate with both actin microfilaments and microtubules (MTs). Structurally, MACF can be divided into 3 domains: an N-terminal domain that contains both a calponin type actin-binding domain and a plakin domain; a rod domain that is co...

journal_title：Journal of cell science

authors： Sun D,Leung CL,Liem RK

更新日期：2001-01-01 00:00:00

abstract：:Analysis by double-label indirect immunofluorescence of PtK1 and HeLa cells had previously demonstrated that prosome* antigens form networks that superimpose on those of the intermediate filaments of the cytokeratin type. We show here that in PtK1 cells various prosomal antigens also reside to a variable extent on int...

journal_title：Journal of cell science

authors： Olink-Coux M,Arcangeletti C,Pinardi F,Minisini R,Huesca M,Chezzi C,Scherrer K

更新日期：1994-03-01 00:00:00

abstract：:The LEM domain (for lamina-associated polypeptide, emerin, MAN1 domain) defines a group of nuclear proteins that bind chromatin through interaction of the LEM motif with the conserved DNA crosslinking protein, barrier-to-autointegration factor (BAF). Here, we describe a LEM protein annotated in databases as 'Ankyrin r...

journal_title：Journal of cell science

authors： Brachner A,Braun J,Ghodgaonkar M,Castor D,Zlopasa L,Ehrlich V,Jiricny J,Gotzmann J,Knasmüller S,Foisner R

更新日期：2012-02-15 00:00:00

abstract：:The CHO1 antigen is a mitosis-specific kinesin-like motor located at the interzonal region of the spindle. The human cDNA coding for the antigen contains a domain with sequence similarity to the motor domain of kinesin-like protein (Nislow et al., Nature 359, 543, 1992). Here we cloned cDNAs encoding the CHO1 antigen ...

journal_title：Journal of cell science

authors： Kuriyama R,Dragas-Granoic S,Maekawa T,Vassilev A,Khodjakov A,Kobayashi H

更新日期：1994-12-01 00:00:00

abstract：:Endocytic trafficking is regulated by ubiquitylation (also known as ubiquitination) of cargoes and endocytic machineries. The role of ubiquitylation in lysosomal delivery has been well documented, but its role in the recycling pathway is largely unknown. Here, we report that the ubiquitin (Ub) ligase RFFL regulates ub...

journal_title：Journal of cell science

authors： Sakai R,Fukuda R,Unida S,Aki M,Ono Y,Endo A,Kusumi S,Koga D,Fukushima T,Komada M,Okiyoneda T

更新日期：2019-02-07 00:00:00

abstract：:In many cells, the endoplasmic reticulum (ER) contains segregated smooth and rough domains, but the mechanism of this segregation is unclear. Here, we used a HeLa cell line, inducibly expressing a GFP fusion protein [GFP-b(5)tail] anchored to the ER membrane, as a tool to investigate factors influencing ER organisatio...

journal_title：Journal of cell science

authors： Sprocati T,Ronchi P,Raimondi A,Francolini M,Borgese N

更新日期：2006-08-01 00:00:00

abstract：:Mitochondrial homeostasis reflects a dynamic balance between membrane fission and fusion events thought essential for mitochondrial function. We report here that altered expression of the C. elegans BCL2 homolog CED-9 affects both mitochondrial fission and fusion. Although striated muscle cells lacking CED-9 have no a...

journal_title：Journal of cell science

authors： Tan FJ,Husain M,Manlandro CM,Koppenol M,Fire AZ,Hill RB

更新日期：2008-10-15 00:00:00