Extracellular polyphosphate signals through Ras and Akt to prime Dictyostelium discoideum cells for development.

abstract：:We have applied fluorescence ratio imaging to the analysis of an actin-binding protein concentration relative to F-actin in macrophages, in order to explore the role of a novel (alpha)-actinin isoform, actinin-4, relative to that of the classical isoform, actinin-1. Conventional immunofluorescence images showed that b...

journal_title：Journal of cell science

authors： Araki N,Hatae T,Yamada T,Hirohashi S

更新日期：2000-09-01 00:00:00

abstract：:Alpha4 integrins are used by leukocytes and neural crest derivatives for adhesion and migration during embryogenesis, immune responses and tumour invasion. The pro-migratory activity of alpha4 integrin is mediated in part through the direct binding of the cytoplasmic domain to paxillin. Here, using intermolecular FRET...

journal_title：Journal of cell science

authors： Deakin NO,Bass MD,Warwood S,Schoelermann J,Mostafavi-Pour Z,Knight D,Ballestrem C,Humphries MJ

更新日期：2009-05-15 00:00:00

abstract：:FAS associated factor 1 (FAF1) has been described as an unusual protein that binds to the intracellular portion of the apoptosis signal transducing receptor FAS/Apo-1 and potentiates apoptosis in L-cells. By means of mRNA differential display we have identified the avian homologue (qFAF) as a fibroblast growth factor-...

journal_title：Journal of cell science

authors： Fröhlich T,Risau W,Flamme I

更新日期：1998-08-01 00:00:00

abstract：:In metazoans, cyclin-dependent kinase 7 (CDK7) has essential roles in both the cell-division cycle and transcription, as a CDK-activating kinase (CAK) and as a component of the general transcription factor TFIIH, respectively. Controversy over its double duty has been resolved, but questions remain. First, how does CD...

journal_title：Journal of cell science

authors： Fisher RP

更新日期：2005-11-15 00:00:00

abstract：:The Trypanosoma brucei flagellum is unusual as it is attached along the cell body and contains, in addition to an apparently conventional axoneme, a structure called the paraflagellar rod, which is essential for cell motility. Here, we investigated flagellum behaviour in normal and mutant trypanosome cell lines where ...

journal_title：Journal of cell science

authors： Branche C,Kohl L,Toutirais G,Buisson J,Cosson J,Bastin P

更新日期：2006-08-15 00:00:00

abstract：:Crosstalk between the microtubule (MT) and actin cytoskeletons is fundamental to many cellular processes including cell polarisation and cell motility. Previous work has shown that members of the growth-arrest-specific 2 (GAS2) family mediate the crosstalk between filamentous actin (F-actin) and MTs, but the molecular...

journal_title：Journal of cell science

authors： Stroud MJ,Nazgiewicz A,McKenzie EA,Wang Y,Kammerer RA,Ballestrem C

更新日期：2014-06-15 00:00:00

abstract：:The mixed lineage leukemia (MLL) proto-oncogenic protein is a histone-lysine N-methyltransferase that is produced by proteolytic cleavage and self-association of the respective functionally distinct subunits (MLL(N) and MLL(C)) to form a holocomplex involved in epigenetic transcriptional regulation. On the basis of st...

journal_title：Journal of cell science

authors： Yokoyama A,Ficara F,Murphy MJ,Meisel C,Naresh A,Kitabayashi I,Cleary ML

更新日期：2011-07-01 00:00:00

abstract：:3-Phosphoinositide-dependent protein kinase-1 (PDK1) and phospholipase C (PLC)γ1 are two key enzymes in signal transduction that control several intracellular processes. Despite the fact that PLCγ1 has been investigated for several years, the mechanisms of activation of this enzyme are still not completely clear. Simi...

journal_title：Journal of cell science

authors： Raimondi C,Chikh A,Wheeler AP,Maffucci T,Falasca M

更新日期：2012-07-01 00:00:00

abstract：:A total arrest of cell membrane movement occurred after incubation for 2 h of cultured fibroblasts with the polycation DEAE-dextran. The immobilized cells resumed normal membrane undulations after exposure for 5 min to the polyanion dextran-sulphate. The influence of DEAE-dextran on the electrophoretic mobility, which...

journal_title：Journal of cell science

authors： Ebbesen P,Güttler F

更新日期：1979-06-01 00:00:00

abstract：:The end1 mutant belongs to a group of four vacuolar protein sorting mutants (class C vps) that lack a morphologically distinguishable and functional vacuole. These mutants share several other phenotypes, such as the inability to grow at 37 degrees C or on nonfermentable carbon sources. We show that, as in the case of ...

journal_title：Journal of cell science

authors： Dulić V,Riezman H

更新日期：1990-11-01 00:00:00

abstract：:The movement of cells in small groups, or clusters, was studied in vitro using epithelioid cells from Gordon-Kosswig melanomas (from poecelid fish) and time-lapse cinemicrography. Tumour explants cultured on glass yield cell sheets from which groups of cells separate and become independently motile clusters. These clu...

journal_title：Journal of cell science

authors： Kolega J

更新日期：1981-06-01 00:00:00

abstract：:CDC6, a replication licensing protein, is partially exported to the cytoplasm in human cells through phosphorylation by Cdk during S phase, but a significant proportion remains in the nucleus. We report here that human CDC6 physically interacts with ATR, a crucial checkpoint kinase, in a manner that is stimulated by p...

journal_title：Journal of cell science

authors： Yoshida K,Sugimoto N,Iwahori S,Yugawa T,Narisawa-Saito M,Kiyono T,Fujita M

更新日期：2010-01-15 00:00:00

abstract：:Cytokines are supposed to be mediators in diarrhoeal diseases. The aim of this study is to characterize the effect of tumor necrosis factor-alpha (TNFalpha) on epithelial barrier function in the colonic epithelial cell line HT-29/B6. Active ion transport and barrier function were measured as short-circuit current and ...

journal_title：Journal of cell science

authors： Schmitz H,Fromm M,Bentzel CJ,Scholz P,Detjen K,Mankertz J,Bode H,Epple HJ,Riecken EO,Schulzke JD

更新日期：1999-01-01 00:00:00

abstract：:Endocytic trafficking is regulated by ubiquitylation (also known as ubiquitination) of cargoes and endocytic machineries. The role of ubiquitylation in lysosomal delivery has been well documented, but its role in the recycling pathway is largely unknown. Here, we report that the ubiquitin (Ub) ligase RFFL regulates ub...

journal_title：Journal of cell science

authors： Sakai R,Fukuda R,Unida S,Aki M,Ono Y,Endo A,Kusumi S,Koga D,Fukushima T,Komada M,Okiyoneda T

更新日期：2019-02-07 00:00:00

abstract：:Endo180 was previously characterized as a novel, cell type specific, recycling transmembrane glycoprotein. This manuscript describes the isolation of a full length human Endo180 cDNA clone which was shown to encode a fourth member of a family of proteins comprising the macrophage mannose receptor, the phospholipase A(...

journal_title：Journal of cell science

authors： Sheikh H,Yarwood H,Ashworth A,Isacke CM

更新日期：2000-03-01 00:00:00

abstract：:The sclerotome portion of the differentiating embryonic chick somite becomes infiltrated by neural crest cells prior to its dispersal towards the embryonic axis. This means that sclerotome cells explanted into culture for the purpose of examining their interactions in vitro are contaminated with a proportion of neural...

journal_title：Journal of cell science

authors： Sanders EJ,Cheung E

更新日期：1988-05-01 00:00:00

abstract：:Failure to form proper synapses in mechanosensory hair cells, the sensory cells responsible for hearing and balance, leads to deafness and balance disorders. Ribbons are electron-dense structures that tether synaptic vesicles to the presynaptic zone of mechanosensory hair cells where they are juxtaposed with the post-...

journal_title：Journal of cell science

authors： Suli A,Pujol R,Cunningham DE,Hailey DW,Prendergast A,Rubel EW,Raible DW

更新日期：2016-06-01 00:00:00

abstract：:Heat-shock for 2 hours at 42 degrees C, or the administration for 3 hours of 100 or 150 microM cadmium chloride, inhibited the subsequent proliferation activity, induced the expression of functional differentiation markers, and caused an increase in the amount of the stress-responsive HSP70 protein in U-937 human prom...

journal_title：Journal of cell science

authors： Vilaboa NE,García-Bermejo L,Pérez C,de Blas E,Calle C,Aller P

更新日期：1997-01-01 00:00:00

abstract：:Although the kinase CHK1 is a key player in the DNA damage response (DDR), several studies have recently provided evidence of DDR-independent roles of CHK1, in particular following phosphorylation of its S280 residue. Here, we demonstrate that CHK1 S280 phosphorylation is cell cycle-dependent and peaks during mitosis....

journal_title：Journal of cell science

authors： Adam K,Cartel M,Lambert M,David L,Yuan L,Besson A,Mayeux P,Manenti S,Didier C

更新日期：2018-08-10 00:00:00

abstract：:Endothelial cell-based angiogenesis requires activation of survival signals that generate resistance to external apoptotic stimuli, such as tumor necrosis factor-alpha (TNF-alpha), during pathobiologic settings. Mechanisms by which this is achieved are not fully defined. Here, we use a model in which the multifunction...

journal_title：Journal of cell science

authors： Kang-Decker N,Cao S,Chatterjee S,Yao J,Egan LJ,Semela D,Mukhopadhyay D,Shah V

更新日期：2007-02-01 00:00:00

abstract：:Loss of tissue organization is a hallmark of the early stages of cancer, and there is considerable interest in proteins that maintain normal tissue architecture. Prostate epithelial cells cultured in Matrigel form three-dimensional acini that mimic aspects of prostate gland development. The organization of these struc...

journal_title：Journal of cell science

authors： Romero D,Kawano Y,Bengoa N,Walker MM,Maltry N,Niehrs C,Waxman J,Kypta R

更新日期：2013-04-15 00:00:00

abstract：:In human red blood cells, protein 4.1 (4.1R) stabilizes the spectrin-actin network and anchors it to the plasma membrane. To contribute to the characterization of functional roles of 4.1R in nonerythroid cells, we analysed the effect of ectopic expression of 4.1R isoforms on interphase microtubules in fibroblastic cel...

journal_title：Journal of cell science

authors： Pérez-Ferreiro CM,Vernos I,Correas I

更新日期：2004-12-01 00:00:00

abstract：:Basonuclin is a zinc finger protein mainly expressed in keratinocytes of the basal layer of epidermis and the outer root sheath of hair follicles. It is also found in abundance in the germ cells of testis and ovary. In cultured keratinocytes, basonuclin is associated with chromatin in all phases of the cell cycle, inc...

journal_title：Journal of cell science

authors： Tseng H,Biegel JA,Brown RS

更新日期：1999-09-01 00:00:00

abstract：:KLP61F in Drosophila and other BimC kinesins are essential for spindle bipolarity across species; loss of BimC function generates high frequencies of monopolar spindles. Concomitant loss of Kar3 kinesin function increases the frequency of bipolar spindles although the underlying mechanism is not known. Recent studies ...

journal_title：Journal of cell science

authors： Wilson PG,Simmons R,Saighal S

更新日期：2004-10-01 00:00:00

abstract：:Low doses of the muscarinic agonist pilocarpine (0.1-1 microM) and the beta-adrenergic agonist isoproterenol (0.5-4 nM) stimulate a minor regulated secretory pathway for salivary proteins in rat parotid lobules. Newly synthesized proteins (labeled biosynthetically) are selectively discharged, and they are secreted in ...

journal_title：Journal of cell science

authors： Castle JD,Castle AM

更新日期：1996-10-01 00:00:00

abstract：:Protein kinase CK2, a vital, pleiotropic and highly conserved serine/threonine phosphotransferase is involved in transcription-directed signaling, gene control and cell cycle regulation and is suspected to play a role in global processes. Searching for these global roles, we analyzed the involvement of CK2 in gene exp...

journal_title：Journal of cell science

authors： Barz T,Ackermann K,Dubois G,Eils R,Pyerin W

更新日期：2003-04-15 00:00:00

abstract：:The development or maturation of intercellular adhesions following their initiation has received very little attention even though this is an area of significance for a variety of in vivo processes. Using Ca2(+)-induced desmosome formation in MDCK cells as a study system it is shown that, following its initiation, des...

journal_title：Journal of cell science

authors： Mattey DL,Burdge G,Garrod DR

更新日期：1990-12-01 00:00:00

abstract：:The mechanisms by which volatile general anaesthetics (VAs) produce a depression of central nervous system are beginning to be better understood, but little is known about a number of side effects. Here, we show that the cold receptor transient receptor potential melastatin 8 (TRPM8) undergoes a complex modulation by ...

journal_title：Journal of cell science

authors： Vanden Abeele F,Kondratskyi A,Dubois C,Shapovalov G,Gkika D,Busserolles J,Shuba Y,Skryma R,Prevarskaya N

更新日期：2013-10-01 00:00:00

abstract：:The tandemly organised ribosomal DNA (rDNA) repeats are transcribed by a dedicated RNA polymerase in a specialised nuclear compartment, the nucleolus. There appears to be an intimate link between the maintenance of nucleolar structure and the presence of heterochromatic chromatin domains. This is particularly evident ...

journal_title：Journal of cell science

authors： Akhmanova A,Verkerk T,Langeveld A,Grosveld F,Galjart N

更新日期：2000-12-01 00:00:00

abstract：:Clathrin-mediated endocytosis (CME) is a crucial cellular process implicated in many aspects of plant growth, development, intra- and intercellular signaling, nutrient uptake and pathogen defense. Despite these significant roles, little is known about the precise molecular details of how CME functions in planta To fac...

journal_title：Journal of cell science

authors： Johnson A,Gnyliukh N,Kaufmann WA,Narasimhan M,Vert G,Bednarek SY,Friml J

更新日期：2020-08-06 00:00:00