Abstract：
:The CHO1 antigen is a mitosis-specific kinesin-like motor located at the interzonal region of the spindle. The human cDNA coding for the antigen contains a domain with sequence similarity to the motor domain of kinesin-like protein (Nislow et al., Nature 359, 543, 1992). Here we cloned cDNAs encoding the CHO1 antigen by immunoscreening of a CHO Uni-Zap expression library, the same species in which the original monoclonal antibody was raised. cDNAs of CHO cells encode a 953 amino acid polypeptide with a calculated molecular mass of 109 kDa. The N-terminal 73% of the antigen was 87% identical to the human clone, whereas the remaining 27% of the coding region showed only 48% homology. Insect Sf9 cells infected with baculovirus containing the full-length insert produced 105 and 95 kDa polypeptides, the same doublet identified as the original antigen in CHO cells. Truncated polypeptides corresponding to the N-terminal motor and C-terminal tail produced a 56 and 54 kDa polypeptide in Sf9 cells, respectively. Full and N-terminal proteins co-sedimented with, and caused bundling of, brain microtubules in vitro, whereas the C-terminal polypeptide did not. Cells expressing the N terminus formed one or more cytoplasmic processes. Immunofluorescence as well as electron microscopic observations revealed the presence of thick bundles of microtubules, which were closely packed, forming a marginal ring just beneath the cell membrane and a core in the processes. The diffusion coefficient and sedimentation coefficient were determined for the native CHO1 antigen by gel filtration and sucrose density gradient centrifugation, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Kuriyama R,Dragas-Granoic S,Maekawa T,Vassilev A,Khodjakov A,Kobayashi Hsubject
Has Abstractpub_date
1994-12-01 00:00:00pages
3485-99eissn
0021-9533issn
1477-9137journal_volume
107 ( Pt 12)pub_type
杂志文章abstract：:The molecular motor dynein is essential for mitotic spindle orientation, which defines the axis of cell division. The light intermediate chain subunits, LIC1 and LIC2, define biochemically and functionally distinct vertebrate dynein complexes, with LIC2-dynein playing a crucial role in ensuring spindle orientation. We...
journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2020-06-23 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2012-03-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：1979-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：1995-01-01 00:00:00
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journal_title：Journal of cell science
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更新日期：1997-01-01 00:00:00
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journal_title：Journal of cell science
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更新日期：1998-07-01 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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更新日期：1992-05-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03170
更新日期：2006-10-15 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1980-02-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1981-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00475
更新日期：2003-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01419
更新日期：2004-10-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00808
更新日期：2003-12-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.041178
更新日期：2009-03-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.082644
更新日期：2011-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.212977
更新日期：2018-07-27 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03474
更新日期：2007-08-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章,收录出版
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更新日期：2006-01-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-10-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2005-05-15 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1984-12-01 00:00:00
abstract：:Murine mesenchymal stem cells are capable of differentiating in vitro into different lineages under stimulation with certain cytokines, growth factors and chemicals. However, the true capacity of these cells to contribute to different cell-types in vivo is still unclear, especially under minimal injury conditions. In ...
journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2004-11-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2003-01-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.040709
更新日期：2009-05-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:During mouse meiosis, the early prophase RAD51/DMC1 recombination protein sites, which are associated with the chromosome cores and which serve as markers for ongoing DNA-DNA interactions, are in ten-fold excess of the eventual reciprocal recombinant events. Most, if not all, of these early interactions are eliminated...
journal_title：Journal of cell science
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更新日期：2002-04-15 00:00:00
abstract：:Using a polyclonal antibody against purified zymogen granule membrane components from rat pancreas a cDNA coding for the 29 kDa protein (ZG29p) was identified by immunoscreening of a hormonally stimulated pancreas cDNA library. Western blot analysis suggests that ZG29p is a pancreas-specific protein and immunofluoresc...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-08-01 00:00:00
