N-terminal acetylation and replicative age affect proteasome localization and cell fitness during aging.

abstract：:The myosin regulatory light chain (RLC) of Dictyostelium discoideum is phosphorylated at a single serine site in response to chemoattractant. To investigate the role of the phosphorylation of RLC in both motility and chemotaxis, mutants were generated in which the single phosphorylatable serine was replaced with a non...

journal_title：Journal of cell science

authors： Zhang H,Wessels D,Fey P,Daniels K,Chisholm RL,Soll DR

更新日期：2002-04-15 00:00:00

abstract：:Cellular activities are regulated by environmental stimuli through protein phosphorylation. Target of rapamycin (TOR), a serine/threonine kinase, plays pivotal roles in cell proliferation and cell growth in response to nutrient status. In Schizosaccharomyces pombe, TORC1, which contains Tor2, plays crucial roles in nu...

journal_title：Journal of cell science

authors： Nakashima A,Sato T,Tamanoi F

更新日期：2010-03-01 00:00:00

abstract：:During the assembly of Plasmodium falciparum merozoites within the schizont stage, the parasite synthesizes and positions three sets of secretory vesicles (rhoptries, micronemes and dense granules) that are active during red cell invasion. There are up to 40 micronemes per merozoite, shaped like long-necked bottles, a...

journal_title：Journal of cell science

authors： Bannister LH,Hopkins JM,Dluzewski AR,Margos G,Williams IT,Blackman MJ,Kocken CH,Thomas AW,Mitchell GH

更新日期：2003-09-15 00:00:00

abstract：:A laser light source along with a new method of preparing endosperm cells of Haemanthus katherinae for differential interference contrast (DIC) microscopy has led to increased visibility of kinetochore fibres. Little information is available concerning the behaviour of these fibres during anaphase in living cells. In ...

journal_title：Journal of cell science

authors： Hard R,Allen RD

更新日期：1977-01-01 00:00:00

abstract：:In this paper we examine DNA repair in the mouse. Using stringently applied inhibitors of DNA synthesis (hydroxyurea and 1-beta-D-arabinofuranosylcytosine) after ultraviolet irradiation we have quantitated an early step in the repair process - the frequency of single-strand DNA breaks, products of enzymic incision--in...

journal_title：Journal of cell science

authors： Elliott GC,Johnson RT

更新日期：1983-03-01 00:00:00

abstract：:The function of microtubules depends on their arrangement into highly ordered arrays. Spatio-temporal control over the formation of new microtubules and regulation of their properties are central to the organization of these arrays. The nucleation of new microtubules requires γ-tubulin, an essential protein that assem...

journal_title：Journal of cell science

authors： Teixidó-Travesa N,Roig J,Lüders J

更新日期：2012-10-01 00:00:00

abstract：:Little is known about the molecular mechanisms that regulate the organization of vascular lumen. In this paper we show that lumen formation correlates with endothelial polarization. Adherens junctions (AJs) and VE-cadherin (VEC, encoded by CDH5) are required for endothelial apicobasal polarity in vitro and during embr...

journal_title：Journal of cell science

authors： Lampugnani MG,Orsenigo F,Rudini N,Maddaluno L,Boulday G,Chapon F,Dejana E

更新日期：2010-04-01 00:00:00

abstract：:Members of the PKC superfamily have been implicated in various migratory models and in particular in spatially restricted processes. However, defining the precise local events that underlie the PKC-dependent processes is constrained by the unspecific nature of interventions. Here we address this problem in relation to...

journal_title：Journal of cell science

authors： Boeckeler K,Rosse C,Howell M,Parker PJ

更新日期：2010-08-15 00:00:00

abstract：:Serotonin receptors 5-HT(1A) and 5-HT(7) are highly coexpressed in brain regions implicated in depression. However, their functional interaction has not been established. In the present study we show that 5-HT(1A) and 5-HT(7) receptors form heterodimers both in vitro and in vivo. Foerster resonance energy transfer-bas...

journal_title：Journal of cell science

authors： Renner U,Zeug A,Woehler A,Niebert M,Dityatev A,Dityateva G,Gorinski N,Guseva D,Abdel-Galil D,Fröhlich M,Döring F,Wischmeyer E,Richter DW,Neher E,Ponimaskin EG

更新日期：2012-05-15 00:00:00

abstract：:Cilia and flagella are conserved eukaryotic organelles important for motility and sensory. The RanGTPase, best known for nucleocytoplasmic transport functions, may also play a role in protein trafficking into the specialized flagellar/ciliary compartments, although the regulatory mechanisms controlling Ran activity at...

journal_title：Journal of cell science

authors： Brasseur A,Bayat S,Chua XL,Zhang Y,Zhou Q,Low BC,He CY

更新日期：2014-11-15 00:00:00

abstract：:A fundamental prediction of the hypothesis that critical events in tumour formation are rare is that the tumours develop by clonal expansion. This prediction has been tested by establishing the isozyme content of tumours induced in mosaic animals whose normal tissues contain two distinguishable isozyme activities. The...

journal_title：Journal of cell science

authors： Deamant FD,Iannaccone PM

更新日期：1987-10-01 00:00:00

abstract：:Stress can be temporary or chronic, and mild or acute. Depending on its extent and severity, cells either alter their metabolism, and adopt a new state, or die. Fluctuations in environmental conditions occur frequently, and such stress disturbs cellular homeostasis, but in general, stresses are reversible and last onl...

journal_title：Journal of cell science

authors： Kucherenko MM,Shcherbata HR

更新日期：2018-02-14 00:00:00

abstract：:Plastids in plants and algae evolved from the endosymbiotic integration of a cyanobacterium by a heterotrophic eukaryote. New plastids can only emerge through fission; thus, the synchronization of bacterial division with the cell cycle of the eukaryotic host was vital to the origin of phototrophic eukaryotes. Most of ...

journal_title：Journal of cell science

authors： de Vries J,Gould SB

更新日期：2018-01-29 00:00:00

abstract：:Bone morphogenetic protein (BMP)-6 is a member of the transforming growth factor (TGF)-(&bgr;) superfamily, and is most similar to BMP-5, osteogenic protein (OP)-1/BMP-7, and OP-2/BMP-8. In the present study, we characterized the endogenous BMP-6 signaling pathway during osteoblast differentiation. BMP-6 strongly indu...

journal_title：Journal of cell science

authors： Ebisawa T,Tada K,Kitajima I,Tojo K,Sampath TK,Kawabata M,Miyazono K,Imamura T

更新日期：1999-10-01 00:00:00

abstract：:Yeast Reb1 and its mammalian ortholog TTF1 are conserved Myb-type DNA-binding proteins that bind to specific sites near the 3'-end of rRNA genes (rDNA). Here, they participate in the termination of transcription driven by RNA polymerase I and block DNA replication forks approaching in the opposite direction. We found ...

journal_title：Journal of cell science

authors： Rodríguez-Sánchez L,Rodríguez-López M,García Z,Tenorio-Gómez M,Schvartzman JB,Krimer DB,Hernández P

更新日期：2011-01-01 00:00:00

abstract：:The current paradigm of noncanonical NF-κB signaling suggests that the loss of TRAF2, TRAF3 or cIAP1 and cIAP2 leads to stabilization of NF-κB-inducing kinase (NIK) to activate the noncanonical pathway. Although a crucial role of RIP1 in the TNFα-induced canonical NF-κB pathway has been well established, its involveme...

journal_title：Journal of cell science

authors： Kim JY,Morgan M,Kim DG,Lee JY,Bai L,Lin Y,Liu ZG,Kim YS

更新日期：2011-02-15 00:00:00

abstract：:The telomeric repeat binding factor 2 (TRF2) specifically recognizes TTAGGG tandem repeats at chromosomal ends. Unexpectedly immunofluorescence studies revealed a prominent nucleolar localization of TRF2 in human cells, which appeared as discrete dots with sizes similar to those present in the nucleoplasm. The TRF2 do...

journal_title：Journal of cell science

authors： Zhang S,Hemmerich P,Grosse F

更新日期：2004-08-01 00:00:00

abstract：:Separation of clathrin-coated pits from the plasma membrane, a key event during endocytosis, is thought to be driven by dynamin and the actin cytoskeleton. However, the mechanism for the actin-mediated endocytosis remains elusive. RNA interference-mediated suppression of cortactin, an F-actin binding protein that prom...

journal_title：Journal of cell science

authors： Zhu J,Zhou K,Hao JJ,Liu J,Smith N,Zhan X

更新日期：2005-02-15 00:00:00

abstract：:Expression of a dominant negative fragment of the spectrin family member Syne-1 causes an accumulation of binucleate cells, suggesting a role for this protein in cytokinesis. An association of this fragment with the C-terminal tail domain of the kinesin II subunit KIF3B was identified by yeast two-hybrid and co-precip...

journal_title：Journal of cell science

authors： Fan J,Beck KA

更新日期：2004-02-01 00:00:00

abstract：:Cilia and flagella are eukaryotic organelles involved in multiple cellular functions. The primary cilium is generally non motile and found in numerous vertebrate cell types where it controls key signalling pathways. Despite a common architecture, ultrastructural data suggest some differences in their organisation. Her...

journal_title：Journal of cell science

authors： Molla-Herman A,Ghossoub R,Blisnick T,Meunier A,Serres C,Silbermann F,Emmerson C,Romeo K,Bourdoncle P,Schmitt A,Saunier S,Spassky N,Bastin P,Benmerah A

更新日期：2010-05-15 00:00:00

abstract：:Kindlin-2 is a FERM and PH domain-containing integrin-binding protein that is emerging as an important regulator of integrin activation. How kindlin-2 functions in integrin activation, however, is not known. We report here that kindlin-2 interacts with multiple phosphoinositides, preferentially with phosphatidylinosit...

journal_title：Journal of cell science

authors： Qu H,Tu Y,Shi X,Larjava H,Saleem MA,Shattil SJ,Fukuda K,Qin J,Kretzler M,Wu C

更新日期：2011-03-15 00:00:00

abstract：:New crystal structures of the kinesin motors differ from previously described motor-ADP atomic models, showing striking changes both in the switch I region near the nucleotide-binding cleft and in the switch II or 'relay' helix at the filament-binding face of the motor. The switch I region, present as a short helix fl...

journal_title：Journal of cell science

authors： Kull FJ,Endow SA

更新日期：2002-01-01 00:00:00

abstract：:MOR1, the Arabidopsis thaliana homologue of the Xenopus microtubule-associated protein MAP215, is required for spatial organization of the acentrosomal microtubule arrays of plant cells. To determine how loss of MOR1 function affects microtubule dynamics, we compared various parameters of microtubule dynamics in the t...

journal_title：Journal of cell science

authors： Kawamura E,Wasteneys GO

更新日期：2008-12-15 00:00:00

abstract：:The distribution of anionic sites on the membranes of rat pancreatic B cells and of their storage granules has been studied by the use of a visual probe of cationic ferritin. Membranes of isolated storage granules possessed a net negative charge which was apparently evenly distributed; the number of anionic sites was ...

journal_title：Journal of cell science

authors： Howell SL,Tyhurst M

更新日期：1977-01-01 00:00:00

abstract：:The synaptonemal complex is an elaborate meiosis-specific supramolecular protein assembly that promotes chromosome synapsis and meiotic recombination. We inactivated the meiosis-specific gene Tex12 and found that TEX12 is essential for progression of meiosis in both male and female germ cells. Structural analysis of t...

journal_title：Journal of cell science

authors： Hamer G,Wang H,Bolcun-Filas E,Cooke HJ,Benavente R,Höög C

更新日期：2008-08-01 00:00:00

abstract：:Meckel-Gruber syndrome (MKS) is a severe autosomal recessively inherited disorder caused by mutations in genes that encode components of the primary cilium and basal body. Here we show that two MKS proteins, MKS1 and meckelin, that are required for centrosome migration and ciliogenesis interact with actin-binding isof...

journal_title：Journal of cell science

authors： Dawe HR,Adams M,Wheway G,Szymanska K,Logan CV,Noegel AA,Gull K,Johnson CA

更新日期：2009-08-01 00:00:00

abstract：:Loss-of-function mutations in the genes encoding PINK1 and Parkin (also known as PARK2) are the most common causes of recessive Parkinson's disease. Both together mediate the selective degradation of mitochondrial proteins and whole organelles via the proteasome and the autophagy-lysosome pathway (mitophagy). The mito...

journal_title：Journal of cell science

authors： Fiesel FC,Moussaud-Lamodière EL,Ando M,Springer W

更新日期：2014-08-15 00:00:00

abstract：:The conventional kinesin motor transports many different cargos to specific locations in neurons. How cargos regulate motor function remains unclear. Here we focus on KIF5, the heavy chain of conventional kinesin, and report that the Kv3 (Shaw) voltage-gated K(+) channel, the only known tetrameric KIF5-binding protein...

journal_title：Journal of cell science

authors： Barry J,Xu M,Gu Y,Dangel AW,Jukkola P,Shrestha C,Gu C

更新日期：2013-05-01 00:00:00

abstract：:Quantitative data are presented regarding cell proliferation and migration on (a) collagen films (b) the surface of 3-dimensional gels of native collagen fibres and (c) within the 3-dimensional collagen gel matrix, as part of a study of the effects of the extracellular matrix on cell behaviour. The nature of the colla...

journal_title：Journal of cell science

authors： Schor SL

更新日期：1980-02-01 00:00:00

abstract：:We have used the H-7w subclone of a Chinese hamster ovary cell line (K1) to investigate the role of cell surface architecture (specifically microvilli, blebs, and sheets) in determining the relative agglutinability of a cell line with Con A. Our evidence clearly demonstrates that no specific, immediately recognizable ...

journal_title：Journal of cell science

authors： Noonan KD,Ukena T

更新日期：1978-12-01 00:00:00