Abstract：
:Expression of a dominant negative fragment of the spectrin family member Syne-1 causes an accumulation of binucleate cells, suggesting a role for this protein in cytokinesis. An association of this fragment with the C-terminal tail domain of the kinesin II subunit KIF3B was identified by yeast two-hybrid and co-precipitation assays, suggesting that the role of Syne-1 in cytokinesis involves an interaction with kinesin II. In support of this we found that (1) expression of KIF3B tail domain also gives rise to multinucleate cells, (2) both Syne-1 and KIF3B localize to the central spindle and midbody during cytokinesis in a detergent resistant and ATP sensitive manner and (3) Syne-1 localization is blocked by expression of KIF3B tail. Also, membrane vesicles containing syntaxin associate with the spindle midbody with identical properties. We conclude that Syne-1 and KIF3B function together in cytokinesis by facilitating the accumulation of membrane vesicles at the spindle midbody.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Fan J,Beck KAdoi
10.1242/jcs.00892keywords:
subject
Has Abstractpub_date
2004-02-01 00:00:00pages
619-29issue
Pt 4eissn
0021-9533issn
1477-9137pii
jcs.00892journal_volume
117pub_type
杂志文章abstract：:In culture, cell confluence generates signals that commit actively growing keratinocytes to exit the cell cycle and differentiate to form a stratified epithelium. Using a comparative proteomic approach, we studied this 'confluence switch' and identified a new pathway triggered by cell confluence that regulates basemen...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.096289
更新日期：2012-09-15 00:00:00
abstract：:Monoclonal mouse hybridoma antibodies were obtained for secreted cellular fibronectin (cFn) from A8387 fibrosarcoma cells. One of them, 52-DH1 (DH), reacted exclusively with cFns but not with plasma Fns (pFns) in immunoblotting and solid-phase EIA. The DH antibody also recognized thermolysin cFn fragments and beta-gal...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1987-11-01 00:00:00
abstract：:The essential gene noa (CG 3971; also known as Baldspot) encodes a very long chain fatty acid elongase which is most similar to the mammalian elongase ELOVL6. noa is expressed in the nervous system from embryogenesis on, in imaginal discs, the fat body, malpighian tubules and in the gonads of both sexes. Its function ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.006551
更新日期：2007-08-15 00:00:00
abstract：:Nematocysts are explosive organelles found in all Cnidaria. Explosion of nematocyst capsules is driven by the high pressure within the capsule formed by the high concentration of poly-gamma-glutamate in the capsule matrix. Poly-gamma-glutamate is a polyanion that binds cations tightly, including the fluorescent cation...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-02-15 00:00:00
abstract：:Studies of human cell hybrids have provided evidence that the tumorigenicity of a cervical carcinoma (HeLa) is under the control of a putative tumor suppressor on chromosome 11. Using these human cell hybrids, we found a tumor-associated glycosylation change in the glucose transporter GLUT1, which is an N-linked glyco...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-12-01 00:00:00
abstract：:Compartmentalization of membrane transport and signaling processes is of pivotal importance to eukaryotic cell function. While plasma membrane compartmentalization and dynamics are well known to depend on the scaffolding function of septin GTPases, the roles of septins at intracellular membranes have remained largely ...
journal_title：Journal of cell science
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更新日期：2019-02-01 00:00:00
abstract：:In this paper we examine DNA repair in the mouse. Using stringently applied inhibitors of DNA synthesis (hydroxyurea and 1-beta-D-arabinofuranosylcytosine) after ultraviolet irradiation we have quantitated an early step in the repair process - the frequency of single-strand DNA breaks, products of enzymic incision--in...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1983-03-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.089672
更新日期：2011-11-01 00:00:00
abstract：:The current paradigm of noncanonical NF-κB signaling suggests that the loss of TRAF2, TRAF3 or cIAP1 and cIAP2 leads to stabilization of NF-κB-inducing kinase (NIK) to activate the noncanonical pathway. Although a crucial role of RIP1 in the TNFα-induced canonical NF-κB pathway has been well established, its involveme...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.075770
更新日期：2011-02-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.236505
更新日期：2019-11-14 00:00:00
abstract：:The mitotic spindle has long been recognized to play an essential role in determining the position of the cleavage furrow during cell division, however little is known about the mechanisms involved in this process. One attractive hypothesis is that signals from the spindle may function to induce reorganization of cort...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-08-01 00:00:00
abstract：:Asymmetric cell division can produce daughter cells with different developmental fates and is often accompanied by a difference in cell size. A number of recent genetic and in vivo imaging studies in Drosophila and Caenorhabditis elegans have begun to elucidate the mechanisms underlying the rearrangements of the cytos...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：
更新日期：2002-06-01 00:00:00
abstract：:Basement membranes are complex extracellular matrices contributing to the regulation of growth, migration and differentiation of many cell types. However, little is known about the mechanisms regulating the deposition and assembly of basement membrane from its constituents. We have investigated the role of extracellul...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.234435
更新日期：2019-09-23 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:Voltage-gated Ca2+ (CaV) channels couple membrane depolarization to Ca2+ influx, triggering a range of Ca2+-dependent cellular processes. CaV channels are, therefore, crucial in shaping neuronal activity and function, depending on their individual temporal and spatial properties. Furthermore, many neurotransmitters an...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.207886
更新日期：2017-11-15 00:00:00
abstract：:Genetic analysis in the yeast Schizosaccharomyces pombe has shown that three genes cdc18, cut5, and cdt1, are essential for DNA synthesis and also for the checkpoint control that couples completion of DNA replication to the onset of mitosis. To test whether assembly of the replication initiation complex is an importan...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：:Formins constitute a family of eukaryotic proteins that are considered to function as a cytoskeleton organizer to regulate morphogenesis, cell polarity and cytokinesis. Fhos is a recently identified mammalian formin, which contains the conserved domains FH (formin homology) 1 and FH2 in the middle region and the Dia-a...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00769
更新日期：2003-11-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.033852
更新日期：2008-12-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2004-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.058693
更新日期：2010-01-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
abstract：:Stress can be temporary or chronic, and mild or acute. Depending on its extent and severity, cells either alter their metabolism, and adopt a new state, or die. Fluctuations in environmental conditions occur frequently, and such stress disturbs cellular homeostasis, but in general, stresses are reversible and last onl...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
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更新日期：2018-02-14 00:00:00
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journal_title：Journal of cell science
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更新日期：2007-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01168
更新日期：2004-07-01 00:00:00
abstract：:It is now becoming clear that additional transmembrane components can modify integrin-mediated adhesion. Syndecan-4 is a transmembrane heparan sulfate proteoglycan whose external glycosaminoglycan chains can bind extracellular matrix ligands and whose core protein cytoplasmic domain can signal during adhesion. Two pap...
journal_title：Journal of cell science
pub_type： 评论,杂志文章,评审
doi：
更新日期：1999-10-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2016-07-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.203372
更新日期：2017-07-15 00:00:00
