Abstract：
:In previous work we used immunofluorescent staining with specific antibodies to study the distribution of five desmosomal antigens in the epithelia of different vertebrate animals. We showed that all five antigens were present in all epithelia studied in human, bovine, rat, guinea pig, chick and frog (Rana pipiens) tissues. It was concluded that desmosomes are highly conserved structures. This paper extends those studies: by including three other species, a lizard (Lacerta viridis), the axolotl (Ambystoma mexicanum) and the trout (Salmo trutta), and by looking at several tissues in more detail. The principal results are as follows. The epidermis of all species down to the frog stain with equal intensity for all desmosomal antigens. In the epidermis of axolotl and trout, staining for desmosomal plaque constituents is present, but staining for the desmosomal glycoproteins is greatly reduced or absent. Within mammalian species as well as chick, lizard and frog, staining for the 115 X 10(3) and 100 X 10(3) molecular weight desmosomal glycoproteins is less intense in non-epidermal tissues than in the epidermis, while staining for desmosomal plaque constituents and for the 150 X 10(3) molecular weight glycoprotein is undiminished. It is possible, therefore, that slight differences exist between certain glycoproteins of epidermis and non-epidermal epithelia. The hearts of lower vertebrates (lizard, frog, axolotl and trout) stain only for individual desmosomal plaque antigens. The pillar cells of trout gill stain, adjacent to their collagenous columns, for one desmosomal plaque antigen. There is a fibrous cytoplasmic mat in this position but no desmosomes. Thus one of the desmosomal antigens may have a function outside the desmosome.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Cowin P,Mattey D,Garrod Dsubject
Has Abstractpub_date
1984-03-01 00:00:00pages
119-32eissn
0021-9533issn
1477-9137journal_volume
66pub_type
杂志文章abstract：:In the present study, to further elucidate the molecular events that control neural crest cell migration, we have analyzed in vitro the adhesive and locomotory response of avian trunk neural crest cells to laminin-1 and searched for the integrin receptors involved in this process. Adhesion of crest cells on laminin-1 ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：:During directed cell migration, the movement of the nucleus is coupled to the forward progression of the cell. The microtubule motor cytoplasmic dynein is required for both cell polarization and cell motility. Here, we investigate the mechanism by which dynein contributes to directed migration. Knockdown of dynein slo...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.033878
更新日期：2008-10-01 00:00:00
abstract：:We investigated the link between cell movement and plasma membrane recycling using a fast-acting, temperature-sensitive mutant of the Dictyostelium SecA exocytic protein. Strikingly, most mutant cells become almost paralysed within minutes at the restrictive temperature. However, they can still sense cyclic-AMP (cAMP)...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.072876
更新日期：2010-10-01 00:00:00
abstract：:Schistosomula of Schistosoma mansoni bind human low density lipoproteins (LDL) in a concentration-dependent and saturable manner. The bound LDL could provide phospholipids and sterol to the worm, which cannot synthesize sterol de novo and lacks acyl chain-modifying capability. Here we have used three phospholipid anal...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
abstract：:Adhesion between cells is established by the formation of specialized intercellular junctional complexes, such as desmosomes. Desmosomes contain isoforms of two members of the cadherin superfamily of cell adhesion proteins, desmocollins (Dsc) and desmogleins (Dsg), but their combinatorial roles in desmosome assembly a...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.146316
更新日期：2014-05-15 00:00:00
abstract：:The constant shedding and renewal of epithelial cells maintain the protection of epithelial barriers. Interference with the processes of host cell-cycle regulation and barrier integrity permits the bacterial pathogen Neisseria gonorrhoeae to effectively colonize and invade epithelial cells. Here, we show that a gonoco...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.117721
更新日期：2013-01-01 00:00:00
abstract：:Mutation of the p53 gene is a common occurrence in human breast cancers but is by no means universal. However, even in tumours where the gene is not mutated altered levels of p53 protein are often detected. This is also observed in cell lines derived from human breast cancers. By transfecting such cell lines containin...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-07-01 00:00:00
abstract：:The translationally controlled protein P23 was discovered by the early induction of its rate of synthesis after mitogenic stimulation of mouse fibroblasts. P23 is expressed in almost all mammalian tissues and it is highly conserved between animals, plants and yeast. Based on its amino acid sequence, P23 cannot be attr...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-04-01 00:00:00
abstract：:We have discovered a novel mitosis-specific human autoantigen that arises at the centromeres of prophase chromosomes, but ultimately participates in formation of an organelle that bisects the cell at late anaphase and during telophase. The organelle, discernible as a three-dimensional disc by confocal microscopy, enco...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-07-01 00:00:00
abstract：:We report the first RNAi-induced phenotypes in mammalian cultured cells using RNA interference mediated by duplexes of 21-nt RNAs. The 21 gene products studied have different functions and subcellular localizations. Knockdown experiments monitored by immunofluorescence and immunoblotting show that even major cellular ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-12-01 00:00:00
abstract：:Multivesicular endosomes are present in virtually every eucaryotic cell, where they arise by intra-endosomal budding of the limiting endosomal membrane. Some genetic diseases such as Chediak-Higashi syndrome are characterized by enlarged membrane-filled endosomes. The same altered endosomal morphology can be observed ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01524
更新日期：2004-12-01 00:00:00
abstract：:The organization of the microfilaments in epidermal cells of Vallisneria leaves was investigated with respect to the induction of cytoplasmic streaming (secondary streaming). In many of the epidermal cells, cytoplasm exhibited rotational streaming along the anticlinal wall of the cell after exposure around the anticli...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1981-04-01 00:00:00
abstract：:To clarify the involvement of actin in the formation of the yeast cell wall, reverting protoplasts of Schizosaccharomyces pombe were used as a simple model system. Actin of reverting protoplasts was labeled with rhodamine-conjugated phalloidin and observed by conventional fluorescence microscopy and laser scanning con...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1989-12-01 00:00:00
abstract：:In many cells, the endoplasmic reticulum (ER) contains segregated smooth and rough domains, but the mechanism of this segregation is unclear. Here, we used a HeLa cell line, inducibly expressing a GFP fusion protein [GFP-b(5)tail] anchored to the ER membrane, as a tool to investigate factors influencing ER organisatio...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03058
更新日期：2006-08-01 00:00:00
abstract：:Mechanical cues are essential for the regulation of cell and tissue physiology. Hence, it has become an utmost necessity for cell biologists to account for those mechanical parameters when investigating biological processes and they need devices to manipulate cells accordingly. Here, we report a simple mechanical cell...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.234666
更新日期：2020-01-06 00:00:00
abstract：:Cells experience mechanical stresses in different physiological and pathological settings. Clathrin-coated structures (CCSs) are sensitive to such perturbations in a way that often results in a mechanical impairment of endocytic budding. Compressive stress is a mechanical perturbation that leads to increased membrane ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.239681
更新日期：2020-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1977-06-01 00:00:00
abstract：:Cardiac fibrosis is linked to fibroblast-to-myofibroblast phenoconversion and proliferation but the mechanisms underlying this are poorly understood. Ski is a negative regulator of TGF-β-Smad signaling in myofibroblasts, and might redirect the myofibroblast phenotype back to fibroblasts. Meox2 could alter TGF-β-mediat...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.126722
更新日期：2014-01-01 00:00:00
abstract：:Cystogenesis is a morphological consequence of numerous genetic diseases of the epithelium. In the kidney, the pathogenic mechanisms underlying the program of altered cell and tubule morphology are obscured by secondary effects of cyst expansion. Here, we developed a new 3D tubuloid system to isolate the rapid changes...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.249557
更新日期：2020-07-16 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03072
更新日期：2006-08-01 00:00:00
abstract：:The postnatal mouse ovary is rich in quiescent and early-growing oocytes, each one surrounded by a layer of somatic granulosa cells (GCs) on a basal lamina. As oocytes start to grow the GCs change shape from flattened to cuboidal, increase their proliferation and form multiple layers, providing a unique model for stud...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.036400
更新日期：2008-12-01 00:00:00
abstract：:Microtubule-associated protein 2 (MAP2) is an abundant neuron-specific protein that binds to microtubules through a domain near its carboxyl terminus that contains either three or four similar repeats of a 31 amino acid motif. When expressed in non-neuronal cells by transfection MAP2 stabilises microtubules and induce...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-11-01 00:00:00
abstract：:Extracellular matrix receptors of the integrin family have a dual role in the epidermis, regulating both adhesion and differentiation. Loss of contact with the extracellular matrix causes keratinocytes to become committed to terminal differentiation, and results in a decrease in the ability of the alpha 5 beta 1 integ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-12-01 00:00:00
abstract：:Cartilage oligomeric matrix protein (COMP) is an abundant component in the extracellular matrix (ECM) of load-bearing tissues such as tendons and cartilage. It provides adaptor functions by bridging different ECM structures. We have previously shown that COMP is also a constitutive component of healthy human skin and ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.180216
更新日期：2016-02-15 00:00:00
abstract：:Recently isolated trophoblasts express nitric oxide synthase 2 (NOS-2) and cyclooxygenase 2 (COX-2), decreasing the levels of the corresponding mRNAs when the cells were maintained in culture. The sustained expression of COX-2 and NOS-2 in trophoblasts was dependent on the activation of nuclear factor kappaB (NF-kappa...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-09-01 00:00:00
abstract：:Clathrin-mediated endocytosis (CME) is a crucial cellular process implicated in many aspects of plant growth, development, intra- and intercellular signaling, nutrient uptake and pathogen defense. Despite these significant roles, little is known about the precise molecular details of how CME functions in planta To fac...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.248062
更新日期：2020-08-06 00:00:00
abstract：:To examine the relative roles of chromosomes, spindle poles and microtubules in the formation of the metaphase spindle and metakinesis, I have experimentally placed an extra centrosome-free pronucleus close to a forming bipolar spindle in a living cell. The chromosomes from the extra nucleus induce the formation of an...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-09-01 00:00:00
abstract：:Myofibroblasts, characterised by high expression of alpha-smooth muscle actin (alpha-SMA), are important and transient cells in normal wound healing but are found in increased number in various pathological conditions of the lung including asthma and pulmonary fibrosis. The mechanisms that regulate the myofibroblast p...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-10-01 00:00:00
abstract：:A simplified model of TIRF optics was used to quantitate the relative membrane/substratum separation distances from the spatial pattern of TIRF image brightness. Phase-contrast and total internal reflection fluorescence microscopy (TIRFM) images were collected of bovine aortic endothelial cells (BAEC) plated onto glas...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-10-01 00:00:00
abstract：:The PX domain, which until recently was an orphan domain, has emerged as the latest member of the phosphoinositide-binding module superfamily. Structural studies have revealed that it has a novel fold and identified key residues that interact with the bound phosphoinositide, enabling some prediction of phosphoinositid...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：
更新日期：2002-03-15 00:00:00
