Abstract:
:Extracellular matrix receptors of the integrin family have a dual role in the epidermis, regulating both adhesion and differentiation. Loss of contact with the extracellular matrix causes keratinocytes to become committed to terminal differentiation, and results in a decrease in the ability of the alpha 5 beta 1 integrin to bind fibronectin. We have investigated whether the decrease in ligand-binding ability is reversible and, if so, whether commitment to terminal differentiation can also be reversed. Keratinocytes that had been placed in suspension for 5 hours to induce commitment were compared with the starting population (0 hour cells) in the presence or absence of 8A2, an activating anti-beta 1 antibody. 8A2 IgG or FAb fragments increased the amount of alpha 5 beta 1 in cell extracts that bound to fibronectin-Sepharose and in the presence of 8A2 the amount of bound alpha 5 beta 1 in 0 hour and 5 hour extracts was equal. 8A2 also restored alpha 5 beta 1 function in adhesion assays of intact 5 hour cells. Ca2+, Mg2+ and Mn2+ alone, at concentrations of up to 1 mM, did not increase the adhesiveness of 5 hour cells relative to 0 hour cells; however, the effect of 8A2 on keratinocytes was dependent on Ca2+. Although 8A2 restored alpha 5 beta 1 ligand-binding ability it did not prevent committed cells from withdrawing from the cell cycle and expressing involucrin, a differentiation marker.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Hotchin NA,Kovach NL,Watt FMsubject
Has Abstractpub_date
1993-12-01 00:00:00pages
1131-8eissn
0021-9533issn
1477-9137journal_volume
106 ( Pt 4)pub_type
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2006-09-01 00:00:00
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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更新日期:2012-05-15 00:00:00
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更新日期:2011-10-15 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2004-01-01 00:00:00
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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更新日期:2019-02-28 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2005-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-01-22 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2017-01-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-06-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::Cyclin E is a regulatory subunit of the cdc2-related protein kinase cdk2, which is activated shortly before S-phase entry, thus defining it as a G1 cyclin. We report here the existence of a 43 kDa splice variant of human cyclin E, termed cyclin Es, which lacks 49 amino acids within the cyclin box compared to the known...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-02-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2005-05-01 00:00:00
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
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journal_title:Journal of cell science
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journal_title:Journal of cell science
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journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
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更新日期:2013-10-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1983-07-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
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journal_title:Journal of cell science
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更新日期:2012-03-15 00:00:00
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journal_title:Journal of cell science
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