Abstract:
:Xenopus egg extracts, which support nuclear assembly and DNA replication, were functionally depleted of lamin LIII by inoculating them with monoclonal anti-lamin antibodies. Phase-contrast microscopy and electron-microscopy studies indicated that lamin-depleted extracts supported efficient chromatin decondensation, and assembly of double membrane structures and nuclear pores on demembranated sperm heads. Immunofluorescence microscopy suggests that lamin-antibody complexes are transported across the nuclear membrane but do not assemble into a lamina. These findings were confirmed by immunoblotting analysis of isolated nuclei. Metabolic labelling studies with either biotin-11-dUTP or [32P]dCTP, revealed that nuclei lacking a lamina were unable to initiate DNA replication and that, although such nuclei could import proteins required for DNA replication (e.g. PCNA), these proteins were apparently not organized into replicon clusters.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Meier J,Campbell KH,Ford CC,Stick R,Hutchison CJsubject
Has Abstractpub_date
1991-03-01 00:00:00pages
271-9eissn
0021-9533issn
1477-9137journal_volume
98 ( Pt 3)pub_type
杂志文章abstract::Nuclear actin plays an important role in many processes that regulate gene expression. Cytoplasmic actin dynamics are tightly controlled by numerous actin-binding proteins, but regulation of nuclear actin has remained unclear. Here, we performed a genome-wide RNA interference (RNAi) screen in Drosophila cells to ident...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.169441
更新日期:2015-07-01 00:00:00
abstract::During pre-mRNA splicing, introns are removed and exons are ligated to form an mRNA. Exon choice is determined by different nuclear protein concentrations varying among tissues and cell types or by developmental stage. These can be altered by different cellular circumstances such as physiological stimuli, environmenta...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01720
更新日期:2005-03-15 00:00:00
abstract::Although the kinase CHK1 is a key player in the DNA damage response (DDR), several studies have recently provided evidence of DDR-independent roles of CHK1, in particular following phosphorylation of its S280 residue. Here, we demonstrate that CHK1 S280 phosphorylation is cell cycle-dependent and peaks during mitosis....
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.213116
更新日期:2018-08-10 00:00:00
abstract::Cells need to sense their mechanical environment during the growth of developing tissues and maintenance of adult tissues. The concept of force-sensing mechanisms that act through cell-cell and cell-matrix adhesions is now well established and accepted. Additionally, it is widely believed that force sensing can be med...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.213496
更新日期:2019-07-30 00:00:00
abstract::Secretory carrier membrane proteins (SCAMPs) are proteins of post-Golgi recycling carriers, including regulated secretory organelles. The two major size variants, SCAMP1 (37 kDa) and SCAMP2 (39 kDa), extensively colocalize in membranes of fibroblasts and parotid acinar cells based on immunocytochemistry and velocity c...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1997-07-01 00:00:00
abstract::Co-culture with glial cells and glia-conditioned media can induce blood-brain barrier properties in microvessel endothelial cells and protect against hypoxia-induced blood-brain barrier breakdown. We examined the effect of two types of glia-conditioned media on brain microvessel endothelial cell permeability and tight...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00264
更新日期:2003-02-15 00:00:00
abstract::The development of DNA microarrays to study simultaneously the level of mRNA expressed from thousands of genes offers great promise to cell biologists. Microarrays can be used to gain detailed information about transcriptional changes involved in a specific pathway, potentially leading to the identification of novel c...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:
更新日期:2000-12-01 00:00:00
abstract::The activation of phospholipase C (PLC) is a conserved mechanism of receptor-activated cell signaling at the plasma membrane. PLC hydrolyzes the minor membrane lipid phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], and continued signaling requires the resynthesis and availability of PI(4,5)P2 at the plasma membrane....
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.217257
更新日期:2018-08-03 00:00:00
abstract::In somatic cells, the heterodimeric Structural Maintenance of Chromosomes (SMC) proteins are involved in chromosome condensation and gene dosage compensation (SMC2 and 4), and sister chromatid cohesion and DNA recombination (SMC1 and 3). We report here evidence for an involvement of mammalian SMC1 and SMC3 proteins in...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00
abstract::Mitogen-activated protein kinases (MAPKs) are conserved regulators of proliferation, differentiation and adaptation in eukaryotic cells. Their activity often involves changes in their subcellular localization, indicating an important role for these spatio-temporal dynamics in signal transmission. A striking model illu...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.213462
更新日期:2018-05-04 00:00:00
abstract::During the prometaphase stage of mitosis, the cell builds a bipolar spindle of microtubules that mechanically segregates sister chromatids between two daughter cells in anaphase. The spindle assembly checkpoint (SAC) is a quality control mechanism that monitors proper attachment of microtubules to chromosome kinetocho...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.191353
更新日期:2018-04-12 00:00:00
abstract::Phagocytosis is an actin-based process used by macrophages to clear particles greater than 0.5 microm in diameter. In addition to its role in immunological responses, phagocytosis is also necessary for tissue remodeling and repair. To prevent catastrophic autoimmune reactions, phagocytosis must be tightly regulated. I...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-02-15 00:00:00
abstract::A key step in cell migration is the dynamic formation and disassembly of adhesions at the front and the concomitant movement and release of adhesions in the rear of the cell. Fibroblasts maintained in the absence of serum have stable adhesions within the rear of the cell and exhibit reduced trailing-edge retraction re...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.020941
更新日期:2008-03-15 00:00:00
abstract::Focal adhesion kinase is a recently characterized tyrosine kinase that is concentrated at focal contacts in cultured cells. It is thought to play an important role in the regulation of the integrin-based signal transduction mechanism involved in the assembly of this membrane specialization. In this study, we examined ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00
abstract::Vertebrate nuclear lamins form a multigene family with developmentally controlled expression. In contrast, invertebrates have long been thought to contain only a single lamin, which in Drosophila is the well-characterized lamin Dm0. Recently, however, a Drosophila cDNA clone (pG-IF) has been identified that codes for ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-10-01 00:00:00
abstract::Actins are major eukaryotic cytoskeletal proteins, and they are involved in many important cell functions, including cell division, cell polarity, wound healing and muscle contraction. Despite obvious drawbacks, muscle actin, which is easily purified, is used extensively for biochemical studies of the non-muscle actin...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.213827
更新日期:2018-04-23 00:00:00
abstract::Previously, we have identified a class of retinal degeneration mutants in Drosophila in which the normally transient interaction between arrestin2 (Arr2) and rhodopsin is stabilized and the complexes are rapidly internalized into the cell body by receptor-mediated endocytosis. The accumulation of protein complexes in ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03052
更新日期:2006-08-01 00:00:00
abstract::Cyclin G (CycG) belongs to the atypical cyclins, which have diverse cellular functions. The two mammalian CycG genes, CycG1 and CycG2, regulate the cell cycle in response to cell stress. Detailed analyses of the role of the single Drosophila cycG gene have been hampered by the lack of a mutant. We generated a null mut...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.113902
更新日期:2012-11-15 00:00:00
abstract::Chromatin is no longer considered to be a static structural framework for packaging DNA within the nucleus but is instead believed to be an interactive component of DNA metabolism. The ordered assembly of chromatin produces a nucleoprotein template capable of epigenetically regulating the expression and maintenance of...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:
更新日期:2000-08-01 00:00:00
abstract::Phosphoinositides regulate numerous processes in various subcellular compartments. Whereas many stimuli trigger changes in the plasma-membrane PtdIns(4,5)P(2) concentration, little is known about its precursor, PtdIns(4)P, in particular whether there are stimulus-induced alterations independent of those of PtdIns(4,5)...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.060525
更新日期:2010-05-01 00:00:00
abstract::MAP1B is a developmentally regulated microtubule-associated phosphoprotein that regulates microtubule dynamics in growing axons and growth cones. We used mass spectrometry to map 28 phosphorylation sites on MAP1B, and selected for further study a putative primed GSK3 beta site and compared it with two nonprimed GSK3 b...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.040162
更新日期:2009-07-15 00:00:00
abstract::During vertebrate gastrulation, a well-orchestrated series of morphogenetic changes leads to the formation of the three germ layers: the ectoderm, mesoderm and endoderm. The analysis of gene expression patterns during gastrulation has been central to the identification of genes involved in germ layer formation. Howeve...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02928
更新日期:2006-05-15 00:00:00
abstract::The cellular microenvironment, characterized by an extracellular matrix (ECM), played an essential role in the transition from unicellularity to multicellularity in animals (metazoans), and in the subsequent evolution of diverse animal tissues and organs. A major ECM component are members of the collagen superfamily -...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.203950
更新日期:2018-04-09 00:00:00
abstract::Spindle pole bodies in the fission yeast Schizosaccharomyces pombe are required during meiosis, not only for spindle formation but also for the assembly of forespore membranes. The spo15 mutant is defective in the formation of forespore membranes, which develop into spore envelopes. The spo15(+)gene encodes a protein ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00
abstract::The proteasome is the major degradation machinery of the cell that regulates multiple cellular processes as diverse as cell cycle, signal transduction and gene expression. Recognition and unfolding of target proteins involves the regulatory cap whose base contains six AAA-ATPases that display reverse chaperone activit...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02743
更新日期:2006-01-15 00:00:00
abstract::During the first meiotic prophase, alignment and synapsis of the homologous chromosomes are mediated by the synaptonemal complex. Incorrect assembly of this complex results in cell death, impaired meiotic recombination and formation of aneuploid germ cells. We have identified a novel mouse meiosis-specific protein, TE...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03182
更新日期:2006-10-01 00:00:00
abstract::Cytokines are supposed to be mediators in diarrhoeal diseases. The aim of this study is to characterize the effect of tumor necrosis factor-alpha (TNFalpha) on epithelial barrier function in the colonic epithelial cell line HT-29/B6. Active ion transport and barrier function were measured as short-circuit current and ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::HSP27 and alphaB-crystallin are both members of the small heat shock protein family. alphaB-crystalllin has been proposed to modulate intermediate filaments and recently a mutation in alphaB-crystallin has been identified as the genetic basis of desmin related myopathy. This disease is characterised in its pathology b...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract::Insect ovarian Sf9 cells extend processes with complex morphologies when infected with a recombinant baculovirus encoding the catalytic subunit of protein kinase A. Within the shafts of the processes are abundant microtubules, which, in contrast to those in Sf9 cells expressing the microtubule-associated protein tau, ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1992-08-01 00:00:00
abstract::The cell wall of 24-h zygotes of Fucus serratus is composed of 3 layers--an inner fibrillar layer (sulphated fucan), an outer fibrillar layer (alginic aicd/cellulose) and an exterior amorphous layer (sulphated fucan, alginic acid). The 2 layers containing sulphated fucan are preferentially thickened at the rhizoid pol...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1978-08-01 00:00:00