Microtubule-organizing centres during the cell cycle of 3T3 cells.

abstract：:The position of the nucleus is regulated in different developmental stages and cellular events. During polarization, the nucleus moves away from the future leading edge and this movement is required for proper cell migration. Nuclear movement requires the LINC complex components nesprin-2G and SUN2, which form transme...

journal_title：Journal of cell science

authors： Borrego-Pinto J,Jegou T,Osorio DS,Auradé F,Gorjánácz M,Koch B,Mattaj IW,Gomes ER

更新日期：2012-03-01 00:00:00

abstract：:Genetic analysis in the yeast Schizosaccharomyces pombe has shown that three genes cdc18, cut5, and cdt1, are essential for DNA synthesis and also for the checkpoint control that couples completion of DNA replication to the onset of mitosis. To test whether assembly of the replication initiation complex is an importan...

journal_title：Journal of cell science

authors： D'Urso G,Grallert B,Nurse P

更新日期：1995-09-01 00:00:00

abstract：:Separation of clathrin-coated pits from the plasma membrane, a key event during endocytosis, is thought to be driven by dynamin and the actin cytoskeleton. However, the mechanism for the actin-mediated endocytosis remains elusive. RNA interference-mediated suppression of cortactin, an F-actin binding protein that prom...

journal_title：Journal of cell science

authors： Zhu J,Zhou K,Hao JJ,Liu J,Smith N,Zhan X

更新日期：2005-02-15 00:00:00

abstract：:Notwithstanding the fact that adult bone marrow cell engraftment to epithelial organs seems a somewhat uncommon event, there is no doubt it does occur, and under appropriate conditions of a strong and positive selection pressure these cells will expand clonally and make a significant contribution to tissue replacement...

journal_title：Journal of cell science

authors： Alison MR,Poulsom R,Otto WR,Vig P,Brittan M,Direkze NC,Preston SL,Wright NA

更新日期：2003-02-15 00:00:00

abstract：:By operating as both a subunit of the cadherin complex and a key component of Wnt signalling, β-catenin acts as the lynchpin between cell-cell contact and transcriptional regulation of proliferation, coordinating epithelial tissue homeostasis and regeneration. The integration of multiple growth-regulatory inputs with ...

journal_title：Journal of cell science

authors： Georgopoulos NT,Kirkwood LA,Southgate J

更新日期：2014-07-01 00:00:00

abstract：:Successful implantation requires the synchronization of viable embryonic development with endometrial receptivity. The mechanisms allowing for the initiation of crosstalk between the embryo and the endometrium remain elusive; however, recent studies have revealed that there are alterations in endometrial microRNAs (mi...

journal_title：Journal of cell science

authors： Kang YJ,Lees M,Matthews LC,Kimber SJ,Forbes K,Aplin JD

更新日期：2015-02-15 00:00:00

abstract：:Recent biochemical and molecular approaches have begun to establish the protein interactions that lead to desmosome assembly. To determine whether these associations occur in native desmosomes we have performed ultrastructural localisation of specific domains of the major desmosomal components and have used the result...

journal_title：Journal of cell science

authors： North AJ,Bardsley WG,Hyam J,Bornslaeger EA,Cordingley HC,Trinnaman B,Hatzfeld M,Green KJ,Magee AI,Garrod DR

更新日期：1999-12-01 00:00:00

abstract：:The activation of phospholipase C (PLC) is a conserved mechanism of receptor-activated cell signaling at the plasma membrane. PLC hydrolyzes the minor membrane lipid phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], and continued signaling requires the resynthesis and availability of PI(4,5)P2 at the plasma membrane....

journal_title：Journal of cell science

authors： Balakrishnan SS,Basu U,Shinde D,Thakur R,Jaiswal M,Raghu P

更新日期：2018-08-03 00:00:00

abstract：:Junctional adhesion molecules (JAMs) are members of an immunoglobulin subfamily expressed by leukocytes and platelets as well as by epithelial and endothelial cells, in which they localize to cell-cell contacts and are specifically enriched at tight junctions. The recent identification of extracellular ligands and int...

journal_title：Journal of cell science

authors： Ebnet K,Suzuki A,Ohno S,Vestweber D

更新日期：2004-01-01 00:00:00

abstract：:We have identified a novel autoantibody specificity in scleroderma that we term anti-chromo. These antibodies recognize several chromosomal antigens with apparent molecular mass of between 23 and 25 kDa, as determined by immunoblots. Anti-chromo autoantibodies occur in 10-15% of sera from patients with anti-centromere...

journal_title：Journal of cell science

authors： Saunders WS,Chue C,Goebl M,Craig C,Clark RF,Powers JA,Eissenberg JC,Elgin SC,Rothfield NF,Earnshaw WC

更新日期：1993-02-01 00:00:00

abstract：:The Dlg tumour suppressor protein is intimately involved in the control of cell contact and polarity. Previous studies have shown that hDlg is a target for a number of viral transforming proteins. In particular, the high risk human papillomavirus (HPV) E6 proteins target hDlg for proteasome-mediated degradation, an ac...

journal_title：Journal of cell science

authors： Mantovani F,Massimi P,Banks L

更新日期：2001-12-01 00:00:00

abstract：:We have demonstrated the possibility of cross-talk between E-cadherin and alphav integrins in breast carcinoma cells. Using the function-blocking anti-alphav monoclonal antibody 17E6, applied to monolayer cultures of breast cancer lines, it was found that treatment of cells possessing detergent-insoluble (implying att...

journal_title：Journal of cell science

authors： von Schlippe M,Marshall JF,Perry P,Stone M,Zhu AJ,Hart IR

更新日期：2000-02-01 00:00:00

abstract：:Here we present experimental evidence that phagocytic cells use modulation of specific components of their glycocalyx to regulate their binding capacity. Particles coated with antibodies specific for the CD32 medium affinity IgG receptor were driven along human monocytic THP-1 cells (expressing CD32) in a flow chamber...

journal_title：Journal of cell science

authors： Sabri S,Soler M,Foa C,Pierres A,Benoliel A,Bongrand P

更新日期：2000-05-01 00:00:00

abstract：:Oocytes of Xenopus laevis in pachytene and early diplotene of meiosis have been studied using the Miller spreading technique. Transcription first appears in germinal vesicles 25-40 micrometers in diameter, when the oocyte is in early diplotene. Transcription at this stage consists of arrays of short RNP transcripts, i...

journal_title：Journal of cell science

authors： Hill RS,Macgregor HC

更新日期：1980-08-01 00:00:00

abstract：:Centaurin-alpha(1) is a phosphatidylinositol 3,4,5-trisphosphate binding protein as well as a GTPase activating protein (GAP) for the ADP-ribosylation factor (ARF) family of small GTPases. To further understand its cellular function, we screened a rat brain cDNA library using centaurin-alpha(1) as bait to identify cen...

journal_title：Journal of cell science

authors： Venkateswarlu K,Hanada T,Chishti AH

更新日期：2005-06-01 00:00:00

abstract：:We have investigated the intracellular distribution of insoluble fibronectin in HeLa tumour cells. By indirect immunofluorescence microscopy fibronectin was detected in the nuclear region, but not in the region of the cell surface. Isolated nuclei and isolated nuclear matrices also stained for fibronectin. By quantita...

journal_title：Journal of cell science

authors： Zerlauth G,Wesierska-Gadek J,Sauermann G

更新日期：1988-03-01 00:00:00

abstract：:Nearly all intermediate filament (IF) proteins share two sequence motifs located at the N- and the C-terminal ends of their helical rod domain ('coil 1a' and 'coil 2b', respectively). To examine the structural role of the coil 2b motif, we have performed in vitro assembly studies and in vivo microinjection experiments...

journal_title：Journal of cell science

authors： Kouklis PD,Traub P,Georgatos SD

更新日期：1992-05-01 00:00:00

abstract：:Distributions of ErbB receptors on membranes of SKBR3 breast cancer cells were mapped by immunoelectron microscopy. The most abundant receptor, ErbB2, is phosphorylated, clustered and active. Kinase inhibitors ablate ErbB2 phosphorylation without dispersing clusters. Modest co-clustering of ErbB2 and EGFR, even after ...

journal_title：Journal of cell science

authors： Yang S,Raymond-Stintz MA,Ying W,Zhang J,Lidke DS,Steinberg SL,Williams L,Oliver JM,Wilson BS

更新日期：2007-08-15 00:00:00

abstract：:Cells govern tissue shape by exerting highly regulated forces at sites of matrix adhesion. As the major force-bearing adhesion-receptor protein, integrins have a central role in how cells sense and respond to the mechanics of their surroundings. Recent studies have shown that a key aspect of mechanotransduction is the...

journal_title：Journal of cell science

authors： Puklin-Faucher E,Sheetz MP

更新日期：2009-01-15 00:00:00

abstract：:Micrographs were made of free-swimming hamster spermatozoa using a high intensity xenon flash and two-colour darkground illumination - conditions that permit three-dimensional reconstruction of the instantaneous shape of the flagellum. From the waveforms observed, we constructed an account of the three-dimensional kin...

journal_title：Journal of cell science

authors： Woolley DM,Osborn IW

更新日期：1984-04-01 00:00:00

abstract：:Kindlin-2 is a FERM and PH domain-containing integrin-binding protein that is emerging as an important regulator of integrin activation. How kindlin-2 functions in integrin activation, however, is not known. We report here that kindlin-2 interacts with multiple phosphoinositides, preferentially with phosphatidylinosit...

journal_title：Journal of cell science

authors： Qu H,Tu Y,Shi X,Larjava H,Saleem MA,Shattil SJ,Fukuda K,Qin J,Kretzler M,Wu C

更新日期：2011-03-15 00:00:00

abstract：:Blocking expression of EhCaBP1, a calmodulin-like, four EF-hand protein from the protozoan parasite Entamoeba histolytica, resulted in inhibition of cellular proliferation. In this paper we report that EhCaBP1 is involved in dynamic changes of the actin cytoskeleton. Both endocytosis and phagocytosis were severely imp...

journal_title：Journal of cell science

authors： Sahoo N,Labruyère E,Bhattacharya S,Sen P,Guillén N,Bhattacharya A

更新日期：2004-07-15 00:00:00

abstract：:The protozoan parasite Giardia intestinalis (also known as Giardia lamblia) is a major waterborne pathogen. During its life cycle, Giardia alternates between the actively growing trophozoite, which has two diploid nuclei with low levels of allelic heterozygosity, and the infectious cyst, which has four nuclei and a to...

journal_title：Journal of cell science

authors： Carpenter ML,Assaf ZJ,Gourguechon S,Cande WZ

更新日期：2012-05-15 00:00:00

abstract：:Enterotoxigenic Bacteroides fragilis - organisms that live in the colon - secrete a metalloprotease toxin, B. fragilis toxin. This toxin binds to a specific intestinal epithelial cell receptor and stimulates cell proliferation, which is dependent, in part, on E-cadherin degradation and beta-catenin-T-cell-factor nucle...

journal_title：Journal of cell science

authors： Wu S,Rhee KJ,Zhang M,Franco A,Sears CL

更新日期：2007-06-01 00:00:00

abstract：:The type II transmembrane protein CD98, best known as the heavy chain of the heterodimeric amino acid transporters (HAT), is required for the surface expression and basolateral localization of this transporter complex in polarized epithelial cells. CD98 also interacts with beta1 integrins resulting in an increase in t...

journal_title：Journal of cell science

authors： Cai S,Bulus N,Fonseca-Siesser PM,Chen D,Hanks SK,Pozzi A,Zent R

更新日期：2005-03-01 00:00:00

abstract：:Meckel-Gruber syndrome (MKS) is a severe autosomal recessively inherited disorder caused by mutations in genes that encode components of the primary cilium and basal body. Here we show that two MKS proteins, MKS1 and meckelin, that are required for centrosome migration and ciliogenesis interact with actin-binding isof...

journal_title：Journal of cell science

authors： Dawe HR,Adams M,Wheway G,Szymanska K,Logan CV,Noegel AA,Gull K,Johnson CA

更新日期：2009-08-01 00:00:00

abstract：:Microtubules--polymers of tubulin--perform essential functions, including regulation of cell shape, intracellular transport and cell motility. How microtubules are adapted to perform multiple diverse functions is not well understood. Post-translational modifications of tubulin subunits diversify the outer and luminal ...

journal_title：Journal of cell science

authors： Wloga D,Gaertig J

更新日期：2010-10-15 00:00:00

abstract：:Bone morphogenetic protein (BMP)-6 is a member of the transforming growth factor (TGF)-(&bgr;) superfamily, and is most similar to BMP-5, osteogenic protein (OP)-1/BMP-7, and OP-2/BMP-8. In the present study, we characterized the endogenous BMP-6 signaling pathway during osteoblast differentiation. BMP-6 strongly indu...

journal_title：Journal of cell science

authors： Ebisawa T,Tada K,Kitajima I,Tojo K,Sampath TK,Kawabata M,Miyazono K,Imamura T

更新日期：1999-10-01 00:00:00

abstract：:Increasing evidence indicates that cellular uptake of several molecules can occur independently of functional dynamin, but the molecular players that regulate dynamin-independent endocytosis and the subsequent trafficking steps are still largely unknown. A survival-based short-hairpin (sh) RNA screen using a cell line...

journal_title：Journal of cell science

authors： Krag C,Malmberg EK,Salcini AE

更新日期：2010-12-15 00:00:00

abstract：:Phosphoinositides regulate numerous processes in various subcellular compartments. Whereas many stimuli trigger changes in the plasma-membrane PtdIns(4,5)P(2) concentration, little is known about its precursor, PtdIns(4)P, in particular whether there are stimulus-induced alterations independent of those of PtdIns(4,5)...

journal_title：Journal of cell science

authors： Wuttke A,Sågetorp J,Tengholm A

更新日期：2010-05-01 00:00:00