Abstract:
:The marginal band microtubules of isolated Xenopus erythrocyte cytoskeletons possess the stability properties of non-steady-state microtubules. They are unperturbed by low temperatures, a variety of microtubule inhibitors, hypotonic treatment and the direct action of calcium. These microtubules can be rapidly depolymerized by erythrocyte lysis in the presence of calcium or by exposure of cytoskeletons obtained and washed in calcium-free media to calcium-containing supernatants of other cell lysates. Thus, marginal band microtubules are calcium-sensitive only in the presence of cytoplasm. The calcium-activated disassembly of the marginal band does not appear to be the result of general or tubulin-specific proteolysis and is prevented by the calmodulin inhibitor, trifluoperazine. On sodium dodecyl sulphate/polyacrylamide gels, samples of calcium-induced, marginal band disassembled cytoskeletons are always tubulin-depleted and also possess a new high molecular weight polypeptide doublet that is believed to constitute stable partial degradation products of spectrin. In the presence of calcium, addition of calmodulin and ATP to cytoskeletons washed free of cytoplasm does not initiate marginal band disassembly. Therefore, if calmodulin mediates marginal band disassembly, it requires cytoplasmic binding proteins or cytoplasmic cofactors.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Gambino J,Ross MJ,Weatherbee JA,Gavin RH,Eckhardt RAsubject
Has Abstractpub_date
1985-11-01 00:00:00pages
199-215eissn
0021-9533issn
1477-9137journal_volume
79pub_type
杂志文章abstract::Male infertility has become a worldwide health problem, but the etiologies of most cases are still unknown. SEPT12, a GTP-binding protein, is involved in male fertility. Two SEPT12 mutations (SEPT12(T89M) and SEPT12(D197N)) have been identified in infertile men who have a defective sperm annulus with a bent tail. The ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.158998
更新日期:2015-03-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:
更新日期:2002-01-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.123612
更新日期:2013-08-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1998-09-01 00:00:00
abstract::The surface of the ciliate Dileptus contains locomotor and sensory domains. Each domain contains cilia of its own kind, arranged in a characteristic pattern. Transformation of a portion of the locomotor area into the sensory domain has been studied. Locomotor ciliary units in the transforming area undergo resorption o...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1983-07-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2018-06-27 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2002-03-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.138933
更新日期:2014-01-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2019-01-09 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.051912
更新日期:2009-07-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01615
更新日期:2005-01-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.067330
更新日期:2010-09-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.184267
更新日期:2016-06-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1989-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02795
更新日期:2006-03-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.035071
更新日期:2008-10-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.114231
更新日期:2013-01-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2005-07-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2019-05-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1979-02-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2013-06-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1984-12-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1979-06-01 00:00:00