The glycoprotein of VSV accumulates in a distal Golgi compartment in the presence of CCCP.

abstract：:Several cell types express inducible nitric oxide synthase (NOS2) in response to exogenous insults such as bacterial lipopolysaccharide (LPS) or proinflammatory cytokines. For instance, muscular cells treated with LPS and interferon gamma (IFN-gamma) respond by increasing the mRNA and protein levels of NOS2, and synth...

journal_title：Journal of cell science

authors： Navarro-Lérida I,Portolés MT,Barrientos AA,Gavilanes F,Boscá L,Rodríguez-Crespo I

更新日期：2004-04-01 00:00:00

abstract：:Cytokines, such as interferons, erythropoietin, leptin and most interleukins, signal through type 1 cytokine receptors and activate the canonical JAK-STAT pathway. Aberrant cytokine signalling underlies numerous pathologies and adequate, temporary receptor activation is therefore under tight control. Negative-feedback...

journal_title：Journal of cell science

authors： Wauman J,De Ceuninck L,Vanderroost N,Lievens S,Tavernier J

更新日期：2011-03-15 00:00:00

abstract：:During gestation the epidermis develops from a single layer of ectoderm into a layer of keratinocytes overlaid by a layer of periderm; this is followed by a progressive increase in the number of layers of keratinocytes, until finally the distinct granular and cornified layers characteristic of mature epidermis are for...

journal_title：Journal of cell science

authors： Watt FM,Keeble S,Fisher C,Hudson DL,Codd J,Salisbury JR

更新日期：1989-10-01 00:00:00

abstract：:Protein traffic moving from the endoplasmic reticulum (ER) to the Golgi complex in mammalian cells passes through the tubulovesicular membrane clusters of the ER-Golgi intermediate compartment (ERGIC), the marker of which is the lectin ERGIC-53. The dynamic nature and functional role of the ERGIC have been debated for...

journal_title：Journal of cell science

authors： Appenzeller-Herzog C,Hauri HP

更新日期：2006-06-01 00:00:00

abstract：:Centralspindlin, a complex of the kinesin-6-family member MKLP1 and MgcRacGAP (also known as Kif23 and Racgap1, respectively), is required for cytokinesis and cell-cell junctions. During anaphase, Centralspindlin accumulates at overlapping central spindle microtubules and directs contractile ring formation by recruiti...

journal_title：Journal of cell science

authors： Breznau EB,Murt M,Blasius TL,Verhey KJ,Miller AL

更新日期：2017-05-15 00:00:00

abstract：:Cytoplasmic dynein-2 is a motor protein complex that drives the movement of cargoes along microtubules within cilia, facilitating the assembly of these organelles on the surface of nearly all mammalian cells. Dynein-2 is crucial for ciliary function, as evidenced by deleterious mutations in patients with skeletal abno...

journal_title：Journal of cell science

authors： Vuolo L,Stevenson NL,Mukhopadhyay AG,Roberts AJ,Stephens DJ

更新日期：2020-03-30 00:00:00

abstract：:Circadian rhythms regulate a wide variety of physiological and metabolic processes. The clock machinery comprises complex transcriptional-translational feedback loops that, through the action of specific transcription factors, modulate the expression of as many as 10% of cellular transcripts. This marked change in gen...

journal_title：Journal of cell science

authors： Bellet MM,Sassone-Corsi P

更新日期：2010-11-15 00:00:00

abstract：:HT-29 cells selected by adaptation to 10(-5) M methotrexate (HT-29 MTX) are a homogeneous cell population producing high amounts of mucin. Intracellular mucins and proteoglycans were isolated from these cells by ultracentrifugation of cell lysates on a cesium bromide gradient and further separated by anion-exchange hi...

journal_title：Journal of cell science

authors： Huet G,Kim I,de Bolos C,Lo-Guidice JM,Moreau O,Hemon B,Richet C,Delannoy P,Real FX,Degand P

更新日期：1995-03-01 00:00:00

abstract：:Faithful genome transmission requires a network of pathways coordinating DNA replication to DNA repair and recombination. Here, we used molecular combing to measure the impact of homologous recombination (HR) on the velocity of DNA replication forks. We used three hamster cell lines defective in HR either by overexpre...

journal_title：Journal of cell science

authors： Daboussi F,Courbet S,Benhamou S,Kannouche P,Zdzienicka MZ,Debatisse M,Lopez BS

更新日期：2008-01-15 00:00:00

abstract：:Nemaline myopathy (NM) is a congenital myopathy with an estimated incidence of 150,000 live births. It is caused by mutations in thin filament components, including nebulin, which accounts for about 50% of the cases. The identification of NM cases with nonsense mutations resulting in loss of the extreme C-terminal SH3...

journal_title：Journal of cell science

authors： Yamamoto DL,Vitiello C,Zhang J,Gokhin DS,Castaldi A,Coulis G,Piaser F,Filomena MC,Eggenhuizen PJ,Kunderfranco P,Camerini S,Takano K,Endo T,Crescenzi M,Luther PK,Lieber RL,Chen J,Bang ML

更新日期：2013-12-01 00:00:00

abstract：:When HeLa cells are lysed in solutions containing a non-ionic detergent and 2 M-NaCl, structures are released that retain many of the morphological features of nuclei. These nucleoids contain all the nuclear RNA and DNA but few of the proteins characteristic of chromatin. Their DNA is supercoiled and so intact. Using ...

journal_title：Journal of cell science

authors： Levin JM,Cook PR

更新日期：1981-08-01 00:00:00

abstract：:Assembly of the nuclear envelope (NE) in telophase is essential for higher eukaryotic cells to re-establish a functional nucleus. Time-lapse, FRAP and FRET analyses in human cells showed that barrier-to-autointegration factor (BAF), a DNA-binding protein, assembled first at the distinct ;core' region of the telophase ...

journal_title：Journal of cell science

authors： Haraguchi T,Kojidani T,Koujin T,Shimi T,Osakada H,Mori C,Yamamoto A,Hiraoka Y

更新日期：2008-08-01 00:00:00

abstract：:Nervous system development and function are tightly regulated by metabolic processes, including the metabolism of lipids such as fatty acids. Mutations in long-chain acyl-CoA synthetase 4 (ACSL4) are associated with non-syndromic intellectual disabilities. We previously reported that Acsl, the Drosophila ortholog of m...

journal_title：Journal of cell science

authors： Huang Y,Huang S,Lam SM,Liu Z,Shui G,Zhang YQ

更新日期：2016-11-01 00:00:00

abstract：:The structural events in the stigma and transmitting tissue of Petunia hybrida pistils that accompany compatible and incompatible intraspecific pollinations have been investigated in detail, together with the changes in reserve levels that also take place at this time. Many of these phenomena may be explained in terms...

journal_title：Journal of cell science

authors： Herrero M,Dickinson HG

更新日期：1979-04-01 00:00:00

abstract：:Delayed wound healing caused by inefficient re-epithelialization underlines chronic skin lesions such as those found in diabetes. The dioxin receptor (AhR) modulates cell plasticity and migration and its activation by occupational polycyclic aromatic hydrocarbons (PAHs) results in severe skin lesions such as contact h...

journal_title：Journal of cell science

authors： Carvajal-Gonzalez JM,Roman AC,Cerezo-Guisado MI,Rico-Leo EM,Martin-Partido G,Fernandez-Salguero PM

更新日期：2009-06-01 00:00:00

abstract：:One aspect of human placental function which has not hitherto been studied is the ability of the placenta to digest proteins intracellularly and use the products of hydrolysis to supply its own and foetall complement of hydrolytic enzymes, including the acid proteases cathepsin C and D. We have used trophoblast cells ...

journal_title：Journal of cell science

authors： Contractor SF,Krakauer K

更新日期：1976-08-01 00:00:00

abstract：:Focal adhesion kinase is a recently characterized tyrosine kinase that is concentrated at focal contacts in cultured cells. It is thought to play an important role in the regulation of the integrin-based signal transduction mechanism involved in the assembly of this membrane specialization. In this study, we examined ...

journal_title：Journal of cell science

authors： Baker LP,Daggett DF,Peng HB

更新日期：1994-06-01 00:00:00

abstract：:Collagen binding proteins (CBP) are hydrophobic, cell surface polypeptides, isolated by collagen affinity chromatography. Antibodies to CBPs inhibit the attachment of embryonic chicken heart fibroblasts to native type I collagen fibrils in a dose-dependent manner. The CBP antibodies also induce rounding and detachment...

journal_title：Journal of cell science

authors： Ogle RC,Potts AJ,Yacoe M,Little CD

更新日期：1989-10-01 00:00:00

abstract：:Cell migration involves the dynamic formation and release of cell-substrate adhesions, where the exertion and detection of mechanical forces take place. Members of the calpain family of calcium-dependent proteases are believed to have a central role in these processes, possibly through the regulation of focal adhesion...

journal_title：Journal of cell science

authors： Undyala VV,Dembo M,Cembrola K,Perrin BJ,Huttenlocher A,Elce JS,Greer PA,Wang YL,Beningo KA

更新日期：2008-11-01 00:00:00

abstract：:Triosephosphate isomerase (TPI) is a glycolytic enzyme that converts dihydroxyacetone phosphate (DHAP) into glyceraldehyde 3-phosphate (GAP). Glycolytic enzyme dysfunction leads to metabolic diseases collectively known as glycolytic enzymopathies. Of these enzymopathies, TPI deficiency is unique in the severity of neu...

journal_title：Journal of cell science

authors： Roland BP,Stuchul KA,Larsen SB,Amrich CG,Vandemark AP,Celotto AM,Palladino MJ

更新日期：2013-07-15 00:00:00

abstract：:The relationshio between Con A-receptor mobility and Con A-induced agglutination of Novikoff hepatoma and normal rat liver cells was investigated. Novikoff cells, incubated with fluorescein-labelled Con A at 3 degrees C displayed uniform, ring-like surface fluorescence. Increasing the temperature of the cells to 37 de...

journal_title：Journal of cell science

authors： Mastromarino P,Neri G,Serra A,Walborg EF Jr

更新日期：1980-04-01 00:00:00

abstract：:Axin and conductin (also known as axin2) are structurally related inhibitors of Wnt/β-catenin signalling that promote degradation of β-catenin. Whereas axin is constitutively expressed, conductin is a Wnt target gene implicated in Wnt negative-feedback regulation. Here, we show that axin and conductin differ in their ...

journal_title：Journal of cell science

authors： Bernkopf DB,Hadjihannas MV,Behrens J

更新日期：2015-01-01 00:00:00

abstract：:Cytokinesis in many eukaryotes requires a contractile actomyosin ring that is placed at the division site. In fission yeast, which is an attractive organism for the study of cytokinesis, actomyosin ring assembly and contraction requires the myosin II heavy chain Myo2p. Although myo2-E1, a temperature-sensitive mutant ...

journal_title：Journal of cell science

authors： Palani S,Srinivasan R,Zambon P,Kamnev A,Gayathri P,Balasubramanian MK

更新日期：2018-01-04 00:00:00

abstract：:Nonsense-mediated mRNA decay (NMD) is an evolutionarily conserved mRNA surveillance system that degrades mRNA transcripts that harbour a premature translation-termination codon (PTC), thus reducing the synthesis of truncated proteins that would otherwise have deleterious effects. Although extensive research has identi...

journal_title：Journal of cell science

authors： Fang Y,Bateman JF,Mercer JF,Lamandé SR

更新日期：2013-06-15 00:00:00

abstract：:A universal feature of mitosis is that all chromosomes become aligned at the spindle equator--the halfway point between the two spindle poles--prior to anaphase onset. This migratory event is called congression, and is powered by centromere-bound protein machines called kinetochores. This Commentary aims to document r...

journal_title：Journal of cell science

authors： Auckland P,McAinsh AD

更新日期：2015-09-15 00:00:00

abstract：:Heat shock transcription factor 1 (HSF1) regulates the expression of a wide array of genes, controls the expression of heat shock proteins (HSPs) as well as cell growth. Although acute depletion of HSF1 induces cellular senescence, the underlying mechanisms are poorly understood. Here, we report that HSF1 depletion-in...

journal_title：Journal of cell science

authors： Oda T,Sekimoto T,Kurashima K,Fujimoto M,Nakai A,Yamashita T

更新日期：2018-05-08 00:00:00

abstract：:The model organism Neurospora crassa undergoes programmed cell death when exposed to staurosporine. Here, we show that staurosporine causes defined changes in cytosolic free Ca(2+) ([Ca(2+)]c) dynamics and a distinct Ca(2+) signature that involves Ca(2+) influx from the external medium and internal Ca(2+) stores. We i...

journal_title：Journal of cell science

authors： Gonçalves AP,Cordeiro JM,Monteiro J,Muñoz A,Correia-de-Sá P,Read ND,Videira A

更新日期：2014-09-01 00:00:00

abstract：:Neurofilaments (NFs) are the major intermediate filaments (IFs) of mature neurons. They play important roles in the structure and function of axons. Recently, two mutations in the neurofilament light (NFL) subunit have been identified in families affected by Charcot-Marie-Tooth (CMT) neuropathy type 2. We have charact...

journal_title：Journal of cell science

authors： Perez-Olle R,Leung CL,Liem RK

更新日期：2002-12-15 00:00:00

abstract：:When Swiss 3T3 cells are treated with colcemid for 60 min (0.5-2 micrograms/ml), although the cytoplasmic network of microtubules is considerably reduced, a significant but variable number of microtubules remain. These residual microtubules appear to converge on a single, usually ciliated, organizing centre. In contra...

journal_title：Journal of cell science

authors： Brooks RF,Richmond FN

更新日期：1983-05-01 00:00:00

abstract：:Export out of the endoplasmic reticulum (ER) involves the Sar1 and COPII machinery acting at ER exit sites (ERES). Whether and how cargo proteins are recruited upstream of Sar1 and COPII is unclear. Two models are conceivable, a recruitment model where cargo is actively transported through a transport factor and hande...

journal_title：Journal of cell science

authors： Yonemura Y,Li X,Müller K,Krämer A,Atigbire P,Mentrup T,Feuerhake T,Kroll T,Shomron O,Nohl R,Arndt HD,Hoischen C,Hemmerich P,Hirschberg K,Kaether C

更新日期：2016-10-15 00:00:00