Abstract:
:Previous investigators have examined mitochondrial DNA (mtDNA) in the electron microscope (EM) after extraction from mitochondria and rotary shadowing. We have observed mtDNA in situ by the osmiumammine procedure for specific staining of DNA in the EM. The procedure was modified to improve the regularity of the staining and then applied to the rapidly dividing cells present in mouse duodenal crypts. In the stained sections of these cells, 25% of the mitochondria exhibited discrete reactive filaments. The filaments, whether observed directly or in stereopairs, appeared either irregular or arranged into distinct patterns, some of which were similar to those previously described after rotary shadowing of duplicating mtDNA: namely, simple and double circular figures, displacement loops and supercoiled forms. The filaments could be traced in serial sections of the same mitochondria and, therefore, were not artifacts. Moreover, their disappearance after DNase digestion demonstrated that they were composed of DNA. It is concluded that mtDNA can be visualized by the modified osmium-ammine technique and may show patterns that can be interpreted as phases in its replication.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Liu DF,el-Alfy M,Leblond CPkeywords:
subject
Has Abstractpub_date
1992-04-01 00:00:00pages
785-93eissn
0021-9533issn
1477-9137journal_volume
101 ( Pt 4)pub_type
杂志文章abstract::Changes in cellular behavior that cause epithelial cells to lose adhesiveness, acquire a motile invasive phenotype and metastasize to secondary sites are complex and poorly understood. Molecules that normally function to integrate adhesive spatial information with cytoskeleton dynamics and membrane trafficking probabl...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.031641
更新日期:2008-09-01 00:00:00
abstract::Earlier reports have indicated that the pollen of Eucalyptus is mechanically robust and unusually resistant to the osmotic stress imposed by immersion in water. We have investigated some of the features of the germination mechanism in the pollen of E. rhodantha with a view to clarifying the role of pollen-wall special...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1985-02-01 00:00:00
abstract::Collagen V is a regulatory fibril-forming collagen that forms heterotypic fibrils with collagen I. Deletion of collagen V in the mouse is associated with a lack of fibril assembly in the embryonic mesenchyme, with a resultant lethal phenotype. The current work elucidates the regulatory roles of collagen V during devel...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.091363
更新日期:2011-12-01 00:00:00
abstract::We have examined the pattern of expression of four different matrix metalloproteinases (MMPs), collagenase, stromelysin, 92 kD gelatinase, and 72 kD gelatinase, by primary and passaged cultures of rabbit corneal fibroblasts. Primary cultures of this cell type have previously been shown to reproduce the normal tissue r...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1990-10-01 00:00:00
abstract::Glycosylphosphatidylinositol-anchored proteins on the egg surface have been proposed to play a role in gamete fusion on the basis of in vitro experiments. We tested this hypothesis by asking if oocyte GPI-anchored proteins are required for fertilization in vivo. Oocyte-specific knockout mice were created using the Cre...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00430
更新日期:2003-06-01 00:00:00
abstract::Cutaneous wound healing is accelerated by exogenous mechanical forces and is impaired in TRPC6-knockout mice. Therefore, we designed experiments to determine how mechanical force and TRPC6 channels contribute to wound healing using HaCaT keratinocytes. HaCaT cells were pretreated with hyperforin, a major component of ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.147314
更新日期:2014-10-01 00:00:00
abstract::T cell activation is accompanied by actin-mediated receptor clustering and reorganization of lipid rafts. It has been suggested that costimulatory molecules might be involved in these processes. We here provide evidence that engagement of the adhesion molecule CD44 initiates cytoskeletal rearrangement and membrane reo...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract::The presence of an acetolysis-resistant polymer (sporopollenin) in the cellular slime moulds is demonstrated. This polymer is located on the stalk sheath of fruiting bodies as a bundle of fine fibrils (4-5 nm diameter). The location and structure of sporopollenin in spores are shown to vary considerably, depending upo...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1984-03-01 00:00:00
abstract::The functions of polycomb products extend beyond their well-known activity as transcriptional regulators to include genome duplication processes. Polycomb activities during DNA replication and DNA damage repair are unclear, particularly without induced replicative stress. We have used a cellular model of conditionally...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.173021
更新日期:2015-10-01 00:00:00
abstract::p190 RhoGAP is a tyrosine phosphorylated protein that contains an N-terminal GTP binding domain, a middle domain (MD) that mediates interaction with p120 RasGAP and a C-terminal GTPase-activating protein (GAP) domain that is specific for the &Rgr; family of small GTPases. Evidence is accumulating to suggest that p190 ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-05-01 00:00:00
abstract::Mesenchyme fragments from early wing buds of normal and talpid3 mutant chick embryos were explanted for culture in plastic Petri dishes and the behaviour of individual cells as they moved out on to the plastic surface was studied by time-lapse ciné photography, followed by statistical analysis. Two parameters of cell ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1975-07-01 00:00:00
abstract::Integrins play a key role in regulating endothelial cell survival, migration and differentiated function during angiogenic blood-vessel remodeling. Integrin-linked kinase (ILK) is a multidomain protein that interacts with the cytoplasmic tail of integrin beta subunits and is thought to participate in integrin-mediated...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01331
更新日期:2004-09-01 00:00:00
abstract::Neural progenitors in the vertebrate nervous system are fully polarized epithelial cells, with intercellular junctions at the apical region. These progenitor cells remain within the neuroepithelium throughout neurogenesis, and will ultimately give rise to all the neurons in the mature nervous system. We have addressed...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.03170
更新日期:2006-10-15 00:00:00
abstract::A Mr 60,000 protein of the axonal cortical cytoplasm, which is recognized by a novel monoclonal antibody, is described. The antibody, DR1, was produced by immunizing mice with a soluble extract of bovine brain membranes that is enriched in known membrane cytoskeletal proteins. DR1 recognizes a Mr 60,000 protein in thi...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1989-11-01 00:00:00
abstract::The cohesin complex prevents premature segregation of duplicated chromosomes by providing resistance to the pole-ward pull by spindle microtubules. The centromeric region (or sister kinetochores) bears the majority of this force and undergoes transient separation prior to anaphase, indicative of its elastic nature. A ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02757
更新日期:2006-02-01 00:00:00
abstract::Maternally inherited cytoplasmic bacteria have occasionally been observed in embryos and adults of different strains of several Drosophila species. While there is a considerable body of data on the relationship between bacteria and embryo viability, little is known about the behavior of these bacteria during the early...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-03-01 00:00:00
abstract::Meckel-Gruber syndrome (MKS) is a severe autosomal recessively inherited disorder caused by mutations in genes that encode components of the primary cilium and basal body. Here we show that two MKS proteins, MKS1 and meckelin, that are required for centrosome migration and ciliogenesis interact with actin-binding isof...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.043794
更新日期:2009-08-01 00:00:00
abstract::Atherosclerosis depends on risk factors such as hyperlipidemia, smoking, hypertension and diabetes. Although these risk factors are relatively constant throughout the arterial circulation, atherosclerotic plaques occur at specific sites where flow patterns are disturbed, with lower overall magnitude and complex change...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.138313
更新日期:2013-11-15 00:00:00
abstract::It is now becoming clear that additional transmembrane components can modify integrin-mediated adhesion. Syndecan-4 is a transmembrane heparan sulfate proteoglycan whose external glycosaminoglycan chains can bind extracellular matrix ligands and whose core protein cytoplasmic domain can signal during adhesion. Two pap...
journal_title:Journal of cell science
pub_type: 评论,杂志文章,评审
doi:
更新日期:1999-10-01 00:00:00
abstract::During meiotic prophase in the ciliate Tetrahymena thermophila micronuclei dramatically elongate and form thread-like crescents. The arrangement of the chromosomes within the crescent as well as the timing of chromosome pairing and recombination with respect to the elongation process have been subjects of ongoing deba...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01504
更新日期:2004-11-15 00:00:00
abstract::Podosomes, adhesion structures capable of matrix degradation, have been linked with the ability of cells to perform chemotaxis and invade tissues. Wiskott-Aldrich Syndrome protein (WASp), an effector of the RhoGTPase Cdc42 and a Src family kinase substrate, regulates macrophage podosome formation. In this study, we de...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.051755
更新日期:2009-11-01 00:00:00
abstract::We have explored the biological function of a surface glycoprotein (GP72) of Trypanosoma cruzi by studying a null mutant parasite, generated by targeted gene deletion. GP72 deletion affected parasite morphology in several stages of the life cycle. Insect midgut (epimastigote) forms had a detached flagellum (apomastigo...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
abstract::Transcriptional activation of the major histocompatibility complex (MHC) by IFNgamma is a key step in cell-mediated immunity. At an early stage of IFNgamma induction, chromatin carrying the entire MHC locus loops out from the chromosome 6 territory. We show here that JAK/STAT signalling triggers this higher-order chro...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.012328
更新日期:2007-09-15 00:00:00
abstract::Cadherins are transmembrane glycoproteins involved in Ca2+-dependent cell-cell adhesion. Using L cells expressing one of three functional E-cadherin constructs, the wild-type, a chimeric molecule with alpha-catenin (EalphaC), and a tail-less one, we determined the effect of v-Src expression on E-cadherin-mediated adhe...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-02-01 00:00:00
abstract::The identification and characterization of scaffold and targeting proteins of the ERK/MAP kinase pathway is important to understand the function and intracellular organization of this pathway. The F-actin binding protein leukocyte-specific protein 1 (LSP1) binds to PKCbetaI and expression of B-LSP1, an LSP1 truncate c...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00955
更新日期:2004-04-15 00:00:00
abstract::We have previously demonstrated that the subcellular distribution of the adhesion plaque protein, talin, changes dramatically in human platelets in response to platelet activation (Beckerle et al., J. Cell Biol. 109, 3333-3346, 1989). Talin is uniformly distributed throughout the cytoplasm of resting platelets. Howeve...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
abstract::Membrane-type matrix metalloproteinase 1 (MT1-MMP) is a membrane-anchored matrix metalloproteinase (MMP) that is frequently associated with processes involving tissue remodelling and cell migration. We have examined MT1-MMP expression and subcellular distribution as a function of MCF10A mammary epithelial cell migrati...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2001-08-01 00:00:00
abstract::Calcineurin-NFAT signaling has been shown to control activity-dependent muscle gene regulation and induce a program of gene expression typical of slow oxidative muscle fibers. Following Ca2+-calmodulin stimulation, calcineurin dephosphorylates NFAT proteins and induces their translocation into the nucleus. However, NF...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02875
更新日期:2006-04-15 00:00:00
abstract::The protein kinase inhibitor H-7 prevents the assembly of tight junctions in cultured Madin Darby Canine Kidney (MDCK) epithelial cells (Balda et al. (1991) J. Membr. Biol. 122, 193-202; Nigam et al. (1991) Biochem. Biophys. Res. Commun. 181, 548-553); however, its mechanism of action is unknown. To understand the bas...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract::In the present study, to further elucidate the molecular events that control neural crest cell migration, we have analyzed in vitro the adhesive and locomotory response of avian trunk neural crest cells to laminin-1 and searched for the integrin receptors involved in this process. Adhesion of crest cells on laminin-1 ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1997-11-01 00:00:00