Abstract：
:Cadherins are transmembrane glycoproteins involved in Ca2+-dependent cell-cell adhesion. Using L cells expressing one of three functional E-cadherin constructs, the wild-type, a chimeric molecule with alpha-catenin (EalphaC), and a tail-less one, we determined the effect of v-Src expression on E-cadherin-mediated adhesion. The aggregation of L cells expressing the wild-type or EalphaC chimeric protein, which both interact with p120(ctn), was reduced by v-Src expression, whereas that of L cells expressing the tail-less E-cadherin was not affected by the expression. Tyrosine phosphorylation of p120(ctn) was observed in v-Src-transformed L cells expressing the wild-type or EalphaC chimeric protein, but not in ones expressing the tail-less E-cadherin. Thus, tyrosine phosphorylation of p120(ctn) depends on the complex formation with E-cadherin and the resulting membrane localization. Constitutive phosphorylation of p120(ctn) on serine and threonine residues also depends on the complex formation and membrane localization. Coexpression of the p120(ctn) protein with an N-terminal deletion, which eliminates some potential tyrosine phosphorylation sites, or the protein with a single amino acid substitution (tyrosine at 217 to phenylalanine) resulted in an increase in the aggregation of v-Src-transformed EL and EalphaCL cells. These results indicate that tyrosine phosphorylation of p120(ctn) is involved in the v-Src modulation of E-cadherin-mediated cell adhesion.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Ozawa M,Ohkubo Tkeywords:
subject
Has Abstractpub_date
2001-02-01 00:00:00pages
503-12issue
Pt 3eissn
0021-9533issn
1477-9137journal_volume
114pub_type
杂志文章abstract：:Cell-free extracts of Xenopus eggs support nuclear assembly and DNA replication in vitro. Extracts supplemented with the protein phosphatase inhibitor microcystin-LR displayed various inhibitory effects at different concentrations of the toxin. In the presence of cycloheximide, additions of microcystin did not induce ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Activation and invasion of the vascular endothelium by Staphylococcus aureus is a major cause of sepsis and endocarditis. For endothelial cell invasion, S. aureus triggers actin polymerization through Cdc42, N-WASp (also known as WASL) and the Arp2/3 complex to assemble a phagocytic cup-like structure. Here, we show t...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.186213
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abstract：:Epithelial cell-cell junctions are specialised structures connecting individual cells in epithelial tissues. They are dynamically and functionally linked to the actin cytoskeleton. Disassembly of these junctions is a key event during physiological and pathological processes, but how this influences gene expression is ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.014456
更新日期：2008-04-01 00:00:00
abstract：:Intracellular signal transduction occurs through cascades of reactions involving dozens of proteins that transmit signals from the cell surface, through a crowded cellular environment filled with organelles and a filamentous cytoskeleton, to specific targets. Numerous signaling molecules are immobilized or transiently...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01122
更新日期：2004-06-01 00:00:00
abstract：:BCL6 is a potent transcriptional repressor that plays important roles in germinal center formation, T helper cell differentiation and lymphomagenesis and regulates expression of several chemokine genes in macrophages. In a further investigation of its role in macrophages, we show that BCL6 inactivation in primary bone...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02314
更新日期：2005-05-01 00:00:00
abstract：:The elliptical, anucleate erythrocytes of camels have been examined for the presence of marginal bands and their constituent microtubules. Lysis of erythrocytes under microtubule-stabilizing conditions readily revealed marginal bands in at least 3 % of the cells, as observed by phase-contrast and darkfield light micro...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1979-04-01 00:00:00
abstract：:During angiogenesis, anastomosing capillary sprouts align to form complex three-dimensional networks of new blood vessels. Using an endothelial cell spheroid model that was developed to study endothelial cell differentiation processes, we have devised a novel collagen gel-based three-dimensional in vitro angiogenesis ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-10-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.133538
更新日期：2013-11-01 00:00:00
abstract：:Enterotoxigenic Bacteroides fragilis - organisms that live in the colon - secrete a metalloprotease toxin, B. fragilis toxin. This toxin binds to a specific intestinal epithelial cell receptor and stimulates cell proliferation, which is dependent, in part, on E-cadherin degradation and beta-catenin-T-cell-factor nucle...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03455
更新日期：2007-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00352
更新日期：2003-04-15 00:00:00
abstract：:We describe here the expression during development, tissue distribution and molecular properties of GP90: a major concanavalin A (ConA)-binding glycoprotein present in the neuronal membrane skeleton from chicken brain. GP90 is co-isolated with, and has a similar developmental profile to contactin (previously called GP...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1989-05-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03020
更新日期：2006-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01139
更新日期：2004-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1977-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.078949
更新日期：2011-05-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-10-01 00:00:00
abstract：:The spindle checkpoint detects errors in kinetochore attachment to microtubules and delays anaphase if attachment is improper. The checkpoint is activated by attachment-sensitive components including Mad2 and certain phosphorylated proteins detected by the 3F3/2 antibody. We have studied Mad2 and 3F3/2 immunofluoresce...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-12-01 00:00:00
abstract：:Insect flight muscle has a large component (Tn-H) in the tropomyosin-troponin complex that is not present in vertebrate striated muscle thin filaments. Tn-H is shown by gold/Fab labelling to be present at regular intervals in insect flight muscle thin filaments. The Fab fragment of a monoclonal antibody to Tn-H was co...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
abstract：:When HeLa cells are lysed in solutions containing a non-ionic detergent and 2 M-NaCl, structures are released that retain many of the morphological features of nuclei. These nucleoids contain all the nuclear RNA and DNA but few of the proteins characteristic of chromatin. Their DNA is supercoiled and so intact. Using ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1981-08-01 00:00:00
abstract：:Mutations within connexin50 (Cx50) have been linked to various cataract phenotypes. To determine the mechanism behind cataract formation we used the paired Xenopus oocyte system in conjunction with transfected HeLa cells and genetically engineered mouse models to examine the functional characteristics of gap junctions...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.012237
更新日期：2007-12-01 00:00:00
abstract：:C-CAM is a Ca(2+)-independent cell adhesion molecule (CAM) that mediates intercellular adhesion of isolated rat hepatocytes. It is widely distributed in epithelia, where its presence both at lateral cell borders and on apical cell surfaces suggests that it may have diverse biological functions. Two major isoforms, C-C...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.204537
更新日期：2017-07-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01635
更新日期：2005-02-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.186700
更新日期：2016-10-01 00:00:00
abstract：:The distribution of centromeres in the interphase nuclei of human diploid fibroblasts was analyzed using anti-centromere immunofluorescence and laser scanning confocal microscopoy. The positions of the centromeres were placed within the nuclear chromatin distribution and presented some aspects of the dynamics of nucle...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:Injection of the circular plasmid FV1 (derived from type I bovine papilloma virus) into Xenopus eggs before the start of the first cell cycle dramatically increases the efficiency of plasmid replication once eggs are chemically activated. We call this the preloading effect and report kinetic and quantitative character...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:The plasma membrane Ca2+-ATPase-2 (PMCA2) is expressed in stereocilia of hair cells of the inner ear, whereas PMCA1 is expressed in the basolateral plasma membrane of hair cells. Both extrude excess Ca2+ from the cytosol. They are predicted to contain ten membrane-spanning segments, two large cytoplasmic loops as well...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03030
更新日期：2006-07-15 00:00:00
abstract：:Epidermal growth factor receptor has been localized to the proliferative cell layers in a variety of stratified squamous epithelia. In the current study, the rat cornea was used as an experimental model to determine if epidermal growth factor receptor is concentrated in epithelial stem cells. Epidermal growth factor r...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
abstract：:Recruitment of secretory vesicles to the cell surface is essential for the sustained secretion of insulin in response to glucose. At present, the molecular motors involved in this movement, and the mechanisms whereby they may be regulated, are undefined. To investigate the role of kinesin family members, we labelled d...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00083
更新日期：2002-11-01 00:00:00
