Involvement of conventional kinesin in glucose-stimulated secretory granule movements and exocytosis in clonal pancreatic beta-cells.

abstract：:The microtubule-associated protein tau is a developmentally regulated family of neuronal phosphoproteins that promotes the assembly and stabilization of microtubules. The carboxy-terminal half of the protein contains three copies of an imperfectly repeated sequence; this region has been found to bind microtubules in v...

journal_title：Journal of cell science

authors： Lee G,Rook SL

更新日期：1992-06-01 00:00:00

abstract：:Ca2+ is a second messenger in many physiological and phytopathological processes. Peroxisomes are subcellular compartments with an active oxidative and nitrosative metabolism. Previous studies have demonstrated that peroxisomal nitric oxide (NO) generation is dependent on Ca2+ and calmodulin (CaM). Here, we used Arabi...

journal_title：Journal of cell science

authors： Corpas FJ,Barroso JB

更新日期：2018-01-29 00:00:00

abstract：:Planar cell polarity (PCP) has been demonstrated in the epithelium of organisms from flies to humans. Recent research has revealed that the planar organization of cells requires a conserved set of genes, known as the PCP genes. Tbe PCP proteins Frizzled (Fz) and Dishevelled (Dsh) function as key players in PCP signall...

journal_title：Journal of cell science

authors： Fanto M,McNeill H

更新日期：2004-02-01 00:00:00

abstract：:Septins have been shown to play important roles in cytokinesis in diverse organisms ranging from yeast to mammals. In this study, we show that both the unc-59 and unc-61 loci encode Caenorhabditis elegans septins. Genomic database searches indicate that unc-59 and unc-61 are probably the only septin genes in the C. el...

journal_title：Journal of cell science

authors： Nguyen TQ,Sawa H,Okano H,White JG

更新日期：2000-11-01 00:00:00

abstract：:Integrins mediate cell adhesion and signal transduction at focal adhesions. Here we investigate the roles of integrin beta subunits in the regulation of actin cytoskeletal structure and the activities of Rho and Rac. The overexpression of beta3 integrin in Chinese hamster ovary cells enhances Rho activity and stress f...

journal_title：Journal of cell science

authors： Miao H,Li S,Hu YL,Yuan S,Zhao Y,Chen BP,Puzon-McLaughlin W,Tarui T,Shyy JY,Takada Y,Usami S,Chien S

更新日期：2002-05-15 00:00:00

abstract：:In Saccharomyces cerevisiae, DNA replication stress activates the replication checkpoint, which slows S-phase progression, stabilizes slowed or stalled replication forks, and relieves inhibition of the ribonucleotide reductase (RNR) complex. To identify novel genes that promote cellular viability after replication str...

journal_title：Journal of cell science

authors： Woolstencroft RN,Beilharz TH,Cook MA,Preiss T,Durocher D,Tyers M

更新日期：2006-12-15 00:00:00

abstract：:Triosephosphate isomerase (TPI) is a glycolytic enzyme that converts dihydroxyacetone phosphate (DHAP) into glyceraldehyde 3-phosphate (GAP). Glycolytic enzyme dysfunction leads to metabolic diseases collectively known as glycolytic enzymopathies. Of these enzymopathies, TPI deficiency is unique in the severity of neu...

journal_title：Journal of cell science

authors： Roland BP,Stuchul KA,Larsen SB,Amrich CG,Vandemark AP,Celotto AM,Palladino MJ

更新日期：2013-07-15 00:00:00

abstract：:Neutrophil extracellular traps (NETs) are one of the most intriguing discoveries in immunological research of the past few years. After their first description in 2004, the number of research articles on how NETs affect immunodefense, and also how they contribute to an ever-growing number of diseases, has skyrocketed....

journal_title：Journal of cell science

authors： Neubert E,Meyer D,Kruss S,Erpenbeck L

更新日期：2020-03-10 00:00:00

abstract：:Dishevelled-3 (Dvl3) is a multivalent scaffold protein that is essential to Wnt signaling during development. Although Dvl-based punctae have been visualized by fluorescence microscopy; the physical nature and dynamic character of the such complexes are enigmatic. We use steric-exclusion chromatography, affinity pull-...

journal_title：Journal of cell science

authors： Yokoyama N,Golebiewska U,Wang HY,Malbon CC

更新日期：2010-11-01 00:00:00

abstract：:Basement membranes are complex extracellular matrices contributing to the regulation of growth, migration and differentiation of many cell types. However, little is known about the mechanisms regulating the deposition and assembly of basement membrane from its constituents. We have investigated the role of extracellul...

journal_title：Journal of cell science

authors： Austria MR,Couchman JR

更新日期：1991-06-01 00:00:00

abstract：:Rat small hepatocytes have been shown to proliferate in culture and to form organoids with differentiated hepatocytes in vitro. To evaluate the degree of polarized transporter differentiation of rat small hepatocytes during 9 weeks of culturing, we studied the time-dependent expression and subcellular localization of ...

journal_title：Journal of cell science

authors： Sidler Pfändler MA,Höchli M,Inderbitzin D,Meier PJ,Stieger B

更新日期：2004-08-15 00:00:00

abstract：:Membrane-bound transferrin-like protein (MTf), a glycosylphosphatidylinositol-anchored protein, is expressed at high levels in many tumors and in several fetal and adult tissues including cartilage and the intestine, as well as in the amyloid plaques of Alzheimer's disease, although its role remains unknown. MTf is on...

journal_title：Journal of cell science

authors： Oda R,Suardita K,Fujimoto K,Pan H,Yan W,Shimazu A,Shintani H,Kato Y

更新日期：2003-05-15 00:00:00

abstract：:In S. cerevisiae, spindle orientation is linked to the inheritance of the ;old' spindle pole by the bud. A player in this asymmetric commitment, Bud6p, promotes cortical capture of astral microtubules. Additionally, Bud6p stimulates actin cable formation though the formin Bni1p. A relationship with the second formin, ...

journal_title：Journal of cell science

authors： Delgehyr N,Lopes CS,Moir CA,Huisman SM,Segal M

更新日期：2008-11-15 00:00:00

abstract：:The adaptor protein SETA/CIN85/Ruk is involved in regulating diverse signal transduction pathways, including the internalization of tyrosine kinase receptors via the Cbl ubiquitin ligases, and attenuating PI3K activity by interaction with its regulatory subunit. Here we present evidence for a new aspect of SETA functi...

journal_title：Journal of cell science

authors： Schmidt MHH,Chen B,Randazzo LM,Bogler O

更新日期：2003-07-15 00:00:00

abstract：:Distribution of mitochondria as well as other intracellular organelles in mammalian cells is regulated by interphase microtubules. Here, we demonstrate a role of microtubules in the mitochondrial biogenesis using various microtubule-active drugs and human osteosarcoma cell line 143B cells and rat liver-derived RL-34 c...

journal_title：Journal of cell science

authors： Karbowski M,Spodnik JH,Teranishi M,Wozniak M,Nishizawa Y,Usukura J,Wakabayashi T

更新日期：2001-01-01 00:00:00

abstract：:The translationally controlled tumor protein (TCTP; also known as TPT1 in mammals) is highly conserved and ubiquitously expressed in eukaryotes. It is involved in growth and development, cell cycle progression, protection against cellular stresses and apoptosis, indicating the multifunctional role of the protein. Here...

journal_title：Journal of cell science

authors： Jojic B,Amodeo S,Bregy I,Ochsenreiter T

更新日期：2018-05-10 00:00:00

abstract：:Rickettsia conorii, the causative agent of Mediterranean spotted fever, is able to attach to and invade a variety of cell types both in vitro and in vivo. Although previous studies show that entry of R. conorii into non-phagocytic cells relies on actin polymerization, little else is known about the molecular details g...

journal_title：Journal of cell science

authors： Martinez JJ,Cossart P

更新日期：2004-10-01 00:00:00

abstract：:The human adenocarcinoma cell line Caco-2 was used for studies on the biosynthesis and transport of lysosomal acid alpha-glucosidase in polarized epithelial cells. Metabolic labelling revealed that in Caco-2 cells alpha-glucosidase is synthesized as a precursor form of 110 x 10(3) Mr. This form is converted into a pre...

journal_title：Journal of cell science

authors： Klumperman J,Fransen JA,Boekestijn TC,Oude Elferink RP,Matter K,Hauri HP,Tager JM,Ginsel LA

更新日期：1991-10-01 00:00:00

abstract：:In hepatocytes, PLIN2 is the major protein coating lipid droplets (LDs), an organelle the hepatitis C virus (HCV) hijacks for virion morphogenesis. We investigated the consequences of PLIN2 deficiency on LDs and on HCV infection. Knockdown of PLIN2 did not affect LD homeostasis, likely due to compensation by PLIN3, bu...

journal_title：Journal of cell science

authors： Lassen S,Grüttner C,Nguyen-Dinh V,Herker E

更新日期：2019-01-09 00:00:00

abstract：:Poly(ADP-ribose) polymerase-1 (PARP1) is a nuclear enzyme that can trigger caspase-independent necrosis. Two main mechanisms for this have been proposed: one involving RIP1 and JNK kinases and mitochondrial permeability transition (MPT), the other involving calpain-mediated activation of Bax and mitochondrial release ...

journal_title：Journal of cell science

authors： Douglas DL,Baines CP

更新日期：2014-10-01 00:00:00

abstract：:Podosomes are highly dynamic actin-based structures commonly found in motile and invasive cells such as macrophages, osteoclasts and vascular smooth muscle cells. Here, we have investigated the role of caldesmon, an actin-binding protein, in the formation of podosomes in aortic smooth muscle A7r5 cells induced by the ...

journal_title：Journal of cell science

authors： Eves R,Webb BA,Zhou S,Mak AS

更新日期：2006-05-01 00:00:00

abstract：:Presenilins were first identified as causative factors in early onset, familial Alzheimer's Disease (FAD). They are predicted to encode a highly conserved novel family of eight transmembrane domain proteins with a large hydrophilic loop between TM6 and TM7 that is the site of numerous FAD mutations. Here, we show that...

journal_title：Journal of cell science

authors： Guo Y,Zhang SX,Sokol N,Cooley L,Boulianne GL

更新日期：2000-10-01 00:00:00

abstract：:Anionic phospholipids PI(4,5)P2 and phosphatidylserine (PS) are enriched in the cytosolic leaflet of the plasma membrane where endocytic sites form. In this study, we investigated the roles of PI(4,5)P2 and PS in clathrin-mediated endocytosis (CME) site initiation and vesicle formation in Saccharomyces cerevisiae. Liv...

journal_title：Journal of cell science

authors： Sun Y,Drubin DG

更新日期：2012-12-15 00:00:00

abstract：:Eukaryogenesis - the emergence of eukaryotic cells - represents a pivotal evolutionary event. With a fundamentally more complex cellular plan compared to prokaryotes, eukaryotes are major contributors to most aspects of life on Earth. For decades, we have understood that eukaryotic origins lie within both the Archaea ...

journal_title：Journal of cell science

authors： Dacks JB,Field MC,Buick R,Eme L,Gribaldo S,Roger AJ,Brochier-Armanet C,Devos DP

更新日期：2016-10-15 00:00:00

abstract：:If mitotic-arrested, cold-stored HeLa cells are incubated at 37 degrees C a proportion of the population divides by an aberrant process which we have called subdivision by extrusion. This process has been studied by time-lapse photography and shown to differ from normal cleavage in several respects. The cell surface b...

journal_title：Journal of cell science

authors： Mullinger AM,Johnson RT

更新日期：1976-11-01 00:00:00

abstract：:The functions of polycomb products extend beyond their well-known activity as transcriptional regulators to include genome duplication processes. Polycomb activities during DNA replication and DNA damage repair are unclear, particularly without induced replicative stress. We have used a cellular model of conditionally...

journal_title：Journal of cell science

authors： Bravo M,Nicolini F,Starowicz K,Barroso S,Calés C,Aguilera A,Vidal M

更新日期：2015-10-01 00:00:00

abstract：:Earlier studies have established that the average speed of a replication fork is two to three times slower in early S-phase than in late S-phase and that the intracellular 2'-deoxyribonucleoside 5'-triphosphate pools grow during S-phase. In this study, the effect of the exogenous 2'-deoxyribonucleoside 5'-triphosphate...

journal_title：Journal of cell science

authors： Malínsky J,Koberna K,Stanĕk D,Masata M,Votruba I,Raska I

更新日期：2001-02-01 00:00:00

abstract：:The essential function of the T cell receptor (TCR) is to translate the engagement of peptides on the major histocompatibility complex (pMHC) into appropriate intracellular signals through the associated cluster of differentiation 3 (CD3) complex. The spatial organization of the TCR-CD3 complex in the membrane is thou...

journal_title：Journal of cell science

authors： Goyette J,Nieves DJ,Ma Y,Gaus K

更新日期：2019-02-11 00:00:00

abstract：:The acidic carboxy-terminal regions of alpha- and beta-tubulin subunits are currently thought to be centrally involved in microtubule stability and in microtubule association with a variety of proteins (MAPs) such as MAP2 and tau proteins. Here, pure tubulin microtubules were exposed to subtilisin to produce polymers ...

journal_title：Journal of cell science

authors： Saoudi Y,Paintrand I,Multigner L,Job D

更新日期：1995-01-01 00:00:00

abstract：:Axenically grown cells of D. discoideum Ax-2 harvested in the log phase of growth, cohere rapidly when shaken in phosphate buffer. After 3.5 days in the stationary phase of growth, cells become completely non-cohesive. Although they do not stick to each other, stationary phase cells do stick to both log phase cells an...

journal_title：Journal of cell science

authors： Jaffe AR,Swan AP,Garrod DR

更新日期：1979-06-01 00:00:00