The role of protein phosphorylation in the assembly of a replication competent nucleus: investigations in Xenopus egg extracts using the cyanobacterial toxin microcystin-LR.

abstract：:The centrosome organizes microtubules by controlling nucleation and anchoring processes. In mammalian cells, subdistal appendages of the mother centriole are major microtubule-anchoring structures of the centrosome. It is not known how newly nucleated microtubules are anchored to these appendages. We show here that ni...

journal_title：Journal of cell science

authors： Delgehyr N,Sillibourne J,Bornens M

更新日期：2005-04-15 00:00:00

abstract：:The essential function of the T cell receptor (TCR) is to translate the engagement of peptides on the major histocompatibility complex (pMHC) into appropriate intracellular signals through the associated cluster of differentiation 3 (CD3) complex. The spatial organization of the TCR-CD3 complex in the membrane is thou...

journal_title：Journal of cell science

authors： Goyette J,Nieves DJ,Ma Y,Gaus K

更新日期：2019-02-11 00:00:00

abstract：:The nature, intracellular distribution, and role of proteins synthesized during meiotic maturation of mouse oocytes in vitro have been examined. Proteins synthesized during the initial stages of maturation are concentrated within the nucleus (germinal vesicle) and become intimately associated with the condensing chrom...

journal_title：Journal of cell science

authors： Wassarman PM,Letourneau GE

更新日期：1976-05-01 00:00:00

abstract：:Epithelia are compact tissues comprising juxtaposed cells that function as mechanical and chemical barriers between the body and the environment. This barrier relies, in part, on adhesive contacts within adherens junctions, which are formed and stabilized by E-cadherin and catenin proteins linked to the actomyosin cyt...

journal_title：Journal of cell science

authors： Le Bras S,Le Borgne R

更新日期：2014-12-15 00:00:00

abstract：:We report the first direct analysis of the endogenous β-catenin phosphorylation activity in colon cancer SW480 cells. By comparing parental SW480 cells that harbor a typical truncated adenomatous polyposis coli (APC) form, cells expressing full-length APC and APC-depleted cells, we provide the formal demonstration tha...

journal_title：Journal of cell science

authors： Wang L,Liu X,Gusev E,Wang C,Fagotto F

更新日期：2014-04-15 00:00:00

abstract：:Human erythrocytes suspended in buffered isotonic saline were frozen to the temperature of liquid nitrogen at various cooling rates of 3, 140, 700, 1800, 3500, 8000 and 11 500 deg. C/min. The membrane ultrastructure in the frozen state and the extent of haemolysis after thawing were examined at each cooling rate. As t...

journal_title：Journal of cell science

authors： Fujikawa S

更新日期：1981-06-01 00:00:00

abstract：:Previously, we have identified a class of retinal degeneration mutants in Drosophila in which the normally transient interaction between arrestin2 (Arr2) and rhodopsin is stabilized and the complexes are rapidly internalized into the cell body by receptor-mediated endocytosis. The accumulation of protein complexes in ...

journal_title：Journal of cell science

authors： Orem NR,Xia L,Dolph PJ

更新日期：2006-08-01 00:00:00

abstract：:Export out of the endoplasmic reticulum (ER) involves the Sar1 and COPII machinery acting at ER exit sites (ERES). Whether and how cargo proteins are recruited upstream of Sar1 and COPII is unclear. Two models are conceivable, a recruitment model where cargo is actively transported through a transport factor and hande...

journal_title：Journal of cell science

authors： Yonemura Y,Li X,Müller K,Krämer A,Atigbire P,Mentrup T,Feuerhake T,Kroll T,Shomron O,Nohl R,Arndt HD,Hoischen C,Hemmerich P,Hirschberg K,Kaether C

更新日期：2016-10-15 00:00:00

abstract：:Promyelocytic leukemia nuclear bodies (PML-NBs) are mobile subnuclear organelles formed by PML and Sp100 protein. They have been reported to have a role in transcription, DNA replication and repair, telomere lengthening, cell cycle control and tumor suppression. We have conducted high-resolution 4Pi fluorescence laser...

journal_title：Journal of cell science

authors： Lang M,Jegou T,Chung I,Richter K,Münch S,Udvarhelyi A,Cremer C,Hemmerich P,Engelhardt J,Hell SW,Rippe K

更新日期：2010-02-01 00:00:00

abstract：:The repair of injured tissue must occur rapidly to prevent microbial invasion and maintain tissue integrity. Epithelial tissues in particular, which serve as a barrier against the external environment, must repair efficiently in order to restore their primary function. Here we analyze the effect of different parameter...

journal_title：Journal of cell science

authors： Abreu-Blanco MT,Verboon JM,Liu R,Watts JJ,Parkhurst SM

更新日期：2012-12-15 00:00:00

abstract：:Microtubules--polymers of tubulin--perform essential functions, including regulation of cell shape, intracellular transport and cell motility. How microtubules are adapted to perform multiple diverse functions is not well understood. Post-translational modifications of tubulin subunits diversify the outer and luminal ...

journal_title：Journal of cell science

authors： Wloga D,Gaertig J

更新日期：2010-10-15 00:00:00

abstract：:Nuclear sap, on fixation in glutaraldehyde, forms a fibrous network that resembles chromatin in its dimensions and staining properties. This artifactual network is easily confused with true chromatin fibres in sections of nuclei. With formaldehyde a homogeneous array of beads is produced. These are approximately 10 nm...

journal_title：Journal of cell science

authors： Skaer RJ,Whytock S

更新日期：1977-08-01 00:00:00

abstract：:The CCAAT/enhancer-binding protein beta (C/EBPbeta, also known as CEBPB) was first identified as a regulator of differentiation and inflammatory processes in adipose tissue and liver. Although C/EBPbeta was initially implicated in synaptic plasticity, its function in the brain remains largely unknown. We have previous...

journal_title：Journal of cell science

authors： Cortes-Canteli M,Luna-Medina R,Sanz-Sancristobal M,Alvarez-Barrientos A,Santos A,Perez-Castillo A

更新日期：2008-04-15 00:00:00

abstract：:Chromatin is no longer considered to be a static structural framework for packaging DNA within the nucleus but is instead believed to be an interactive component of DNA metabolism. The ordered assembly of chromatin produces a nucleoprotein template capable of epigenetically regulating the expression and maintenance of...

journal_title：Journal of cell science

authors： Ridgway P,Almouzni G

更新日期：2000-08-01 00:00:00

abstract：:Cytokines are supposed to be mediators in diarrhoeal diseases. The aim of this study is to characterize the effect of tumor necrosis factor-alpha (TNFalpha) on epithelial barrier function in the colonic epithelial cell line HT-29/B6. Active ion transport and barrier function were measured as short-circuit current and ...

journal_title：Journal of cell science

authors： Schmitz H,Fromm M,Bentzel CJ,Scholz P,Detjen K,Mankertz J,Bode H,Epple HJ,Riecken EO,Schulzke JD

更新日期：1999-01-01 00:00:00

abstract：:The signal recognition particle (SRP) is a ribonucleoprotein machine that controls the translation and intracellular sorting of membrane and secreted proteins. The SRP contains a core RNA subunit with which six proteins are assembled. Recent work in both yeast and mammalian cells has identified the nucleolus as a poss...

journal_title：Journal of cell science

authors： Sommerville J,Brumwell CL,Politz JC,Pederson T

更新日期：2005-03-15 00:00:00

abstract：:The bimE (blocked-in-mitosis) gene appears to function as a negative mitotic regulator because the recessive bimE7 mutation can override certain interphase-arresting treatments and mutations, causing abnormal induction of mitosis. We have further investigated the role of bimE in cell cycle checkpoint control by: (1) c...

journal_title：Journal of cell science

authors： James SW,Mirabito PM,Scacheri PC,Morris NR

更新日期：1995-11-01 00:00:00

abstract：:Integrin-mediated cell adhesion is central to cell survival, differentiation and motility. Many cell responses induced by integrins require both receptor occupancy and receptor aggregation, and appear to be regulated by both biochemical and biophysical means. Multidomain extracellular matrix molecules may serve to fos...

journal_title：Journal of cell science

authors： Koo LY,Irvine DJ,Mayes AM,Lauffenburger DA,Griffith LG

更新日期：2002-04-01 00:00:00

abstract：:Every two years, the French Society for Cell Biology (SBCF) organises an international meeting called 'Imaging the Cell'. This year, the 8th edition was held on 24-26 June 2015 at University of Bordeaux Campus Victoire in the city of Bordeaux, France, a UNESCO World Heritage site. Over the course of three days, the me...

journal_title：Journal of cell science

authors： Moreau V,Cordelières FP,Poujol C,Sagot I,Saltel F

更新日期：2015-11-01 00:00:00

abstract：:Homophilic binding of E-cadherins through their ectodomains is fundamental to epithelial cell-cell adhesion. Despite this, E-cadherin ectodomains have evolved differently in the vertebrate and insect lineages. Of the five rod-like, tandemly aligned extracellular cadherin domains of vertebrate E-cadherin, the tip extra...

journal_title：Journal of cell science

authors： Nishiguchi S,Yagi A,Sakai N,Oda H

更新日期：2016-09-01 00:00:00

abstract：:Alix is a cytosolic protein in mammalian cells that was originally identified on the basis of its association with pro-apoptotic signaling. More recent evidence has established that Alix has a hand in regulating other cellular mechanisms, including endocytic membrane trafficking and cell adhesion. Although Alix appear...

journal_title：Journal of cell science

authors： Odorizzi G

更新日期：2006-08-01 00:00:00

abstract：:Cytoplasmic dynein-2 is a motor protein complex that drives the movement of cargoes along microtubules within cilia, facilitating the assembly of these organelles on the surface of nearly all mammalian cells. Dynein-2 is crucial for ciliary function, as evidenced by deleterious mutations in patients with skeletal abno...

journal_title：Journal of cell science

authors： Vuolo L,Stevenson NL,Mukhopadhyay AG,Roberts AJ,Stephens DJ

更新日期：2020-03-30 00:00:00

abstract：:In this paper, we identify the chondroitin sulfate proteoglycan versican V2 as a major inhibitor of axonal growth in the extracellular matrix of the mature central nervous system. In immunohistochemical and in situ hybridization experiments we show that this tissue-specific splice variant of versican is predominantly ...

journal_title：Journal of cell science

authors： Schmalfeldt M,Bandtlow CE,Dours-Zimmermann MT,Winterhalter KH,Zimmermann DR

更新日期：2000-03-01 00:00:00

abstract：:Stress can be temporary or chronic, and mild or acute. Depending on its extent and severity, cells either alter their metabolism, and adopt a new state, or die. Fluctuations in environmental conditions occur frequently, and such stress disturbs cellular homeostasis, but in general, stresses are reversible and last onl...

journal_title：Journal of cell science

authors： Kucherenko MM,Shcherbata HR

更新日期：2018-02-14 00:00:00

abstract：:The position of the nucleus is regulated in different developmental stages and cellular events. During polarization, the nucleus moves away from the future leading edge and this movement is required for proper cell migration. Nuclear movement requires the LINC complex components nesprin-2G and SUN2, which form transme...

journal_title：Journal of cell science

authors： Borrego-Pinto J,Jegou T,Osorio DS,Auradé F,Gorjánácz M,Koch B,Mattaj IW,Gomes ER

更新日期：2012-03-01 00:00:00

abstract：:Tau is a group of neuronal microtubule-associated proteins that are formed by alternative mRNA splicing and accumulate in neurofibrillary tangles in Alzheimer's disease (AD) brain. Tau plays a key role in regulating microtubule dynamics, axonal transport and neurite outgrowth, and all these functions of tau are modula...

journal_title：Journal of cell science

authors： Johnson GV,Stoothoff WH

更新日期：2004-11-15 00:00:00

abstract：:We investigated the link between cell movement and plasma membrane recycling using a fast-acting, temperature-sensitive mutant of the Dictyostelium SecA exocytic protein. Strikingly, most mutant cells become almost paralysed within minutes at the restrictive temperature. However, they can still sense cyclic-AMP (cAMP)...

journal_title：Journal of cell science

authors： Zanchi R,Howard G,Bretscher MS,Kay RR

更新日期：2010-10-01 00:00:00

abstract：:By differential screening of a Xenopus laevis egg cDNA library, we have isolated a 2,111 bp cDNA which corresponds to a maternal mRNA specifically deadenylated after fertilisation. This cDNA, called Eg2, encodes a 407 amino acid protein kinase. The pEg2 sequence shows significant identity with members of a new protein...

journal_title：Journal of cell science

authors： Roghi C,Giet R,Uzbekov R,Morin N,Chartrain I,Le Guellec R,Couturier A,Dorée M,Philippe M,Prigent C

更新日期：1998-03-01 00:00:00

abstract：:Localization and translation of mRNAs within different subcellular domains provides an important mechanism to spatially and temporally introduce new proteins in polarized cells. Neurons make use of this localized protein synthesis during initial growth, regeneration and functional maintenance of their axons. Although ...

journal_title：Journal of cell science

authors： Sahoo PK,Smith DS,Perrone-Bizzozero N,Twiss JL

更新日期：2018-04-13 00:00:00

abstract：:In skeletal muscle, the triad is a structure comprising a transverse (T)-tubule and sarcoplasmic reticulum (SR) cisternae. Triads constitute the basis of excitation-contraction coupling as the cradle of the Ca2+ release complex. We have shown previously that triadin, a member of this complex, has shaping properties on...

journal_title：Journal of cell science

authors： Osseni A,Sébastien M,Sarrault O,Baudet M,Couté Y,Fauré J,Fourest-Lieuvin A,Marty I

更新日期：2016-10-15 00:00:00