Abstract：
:Cells depend on a highly ordered organisation of their content and must develop strategies to maintain the anisotropic distribution of organelles during periods of nutrient shortage. One of these strategies is to solidify the cytoplasm, which was observed in bacteria and yeast cells with acutely interrupted energy production. Here, we describe a different type of cytoplasm solidification fission yeast cells switch to, after having run out of nutrients during multiple days in culture. It provides the most profound reversible cytoplasmic solidification of yeast cells described to date. Our data exclude the previously proposed mechanisms for cytoplasm solidification in yeasts and suggest a mechanism that immobilises cellular components in a size-dependent manner. We provide experimental evidence that, in addition to time, cells use intrinsic nutrients and energy sources to reach this state. Such cytoplasmic solidification may provide a robust means to protect cellular architecture in dormant cells.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Heimlicher MB,Bächler M,Liu M,Ibeneche-Nnewihe C,Florin EL,Hoenger A,Brunner Ddoi
10.1242/jcs.231688subject
Has Abstractpub_date
2019-11-08 00:00:00issue
21eissn
0021-9533issn
1477-9137pii
jcs.231688journal_volume
132pub_type
杂志文章abstract：:To investigate the mechanism of chromatin assembly at human centromeres, we isolated cultured human cell lines in which a transfected alpha-satellite (alphoid) YAC was integrated ectopically into the terminal region of host chromosome 16, where it was stably maintained. Centromere activity of the alphoid YAC was suppr...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00697
更新日期：2003-10-01 00:00:00
abstract：:Previously, we showed that an integral component of stored mRNP particles in Xenopus oocytes, Xp54, is a DEAD-box RNA helicase with ATP-dependent RNA-unwinding activity. Xp54 belongs to small family of helicases (DDX6) that associate with mRNA molecules encoding proteins required for progress through meiosis. Here we ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-01-15 00:00:00
abstract：:This project examines the transcellular membrane protein polarity of bovine aortic endothelial cell (BAEC) monolayers in vitro with respect to the roles that intercellular junctions (as defined by comparing confluent and subconfluent monolayers) and the submembranous cytoskeleton play in controlling this phenomenon. P...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-09-01 00:00:00
abstract：:Epitectin, the mucin-like glycoprotein defined by the monoclonal antibodies CA1, CA2 and CA3, has been examined by electron microscopy to determine its shape and size. It appears to be a single extended strand with a mean length of about 270 nm. The antibodies CA1 and CA2 appear to bind preferentially to a terminal si...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1986-12-01 00:00:00
abstract：:The monoclonal antibody Bx63 recognizes a centrosomal antigen of Drosophila melanogaster by indirect immunofluorescence and identifies two proteins, with apparent molecular weights of 185 x 10(3) and 66 x 10(3), on Western blots. We have used this antibody to isolate five clones (lambda cs1, -2, -3, -4 and lambda j63)...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1988-04-01 00:00:00
abstract：:Apical endocytosis of 125I-ricin in Caco-2 cells was inhibited > 95% by hypertonic and/or acid media, consistent with the major uptake route being clathrin-mediated. The presence of apical cell surface bound ricin-gold in clathrin coated pits and vesicles was observed by electron microscopy. An electron microscopic in...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-12-01 00:00:00
abstract：:Fluorescence in situ hybridization in combination with synaptonemal complex and spindle pole body immunostaining to both spread and structurally preserved nuclei from time course experiments disclosed prominent telomere clustering during meiotic prophase of the yeast Saccharomyces cerevisiae. It was found that centrom...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：:Dyneins are motor proteins that move along microtubules. They have many roles in the cell. They drive the beating of cilia and flagella, move cargos in the cytoplasm and function in the mitotic spindle. Dyneins are large and complex protein machines. Until recently, the way they move was poorly understood. In 2012, tw...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.120725
更新日期：2013-02-01 00:00:00
abstract：:A method is reported for preparing surface (plasma) membranes from adrenal tumour (Y-1) cells. The procedure is based upon homogenization in hypotonic ZnCl2 followed by sedimentation through two sucrose density gradients. The purified membranes consist of large sheets of membrane. The identity and purity of the membra...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1985-08-01 00:00:00
abstract：:Assembly of the nuclear envelope (NE) in telophase is essential for higher eukaryotic cells to re-establish a functional nucleus. Time-lapse, FRAP and FRET analyses in human cells showed that barrier-to-autointegration factor (BAF), a DNA-binding protein, assembled first at the distinct ;core' region of the telophase ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.033597
更新日期：2008-08-01 00:00:00
abstract：:Laminins are heterotrimeric glycoproteins of the extracellular matrix that are secreted by epithelial cells and which are crucial for the normal structure and function of the basement membrane. We have generated a mouse harboring a conditional knockout of α3 laminin (Lama3(fl/fl)), one of the main laminin subunits in ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.080911
更新日期：2011-09-01 00:00:00
abstract：:There is a limited number of methods to examine transbilayer lipid distribution in biomembranes. We employed freeze-fracture replica-labelling immunoelectron microscopy in combination with lipid-binding proteins and a peptide to examine both transbilayer distribution and lateral distribution of various phospholipids i...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.163105
更新日期：2015-04-15 00:00:00
abstract：:Endo180 was previously characterized as a novel, cell type specific, recycling transmembrane glycoprotein. This manuscript describes the isolation of a full length human Endo180 cDNA clone which was shown to encode a fourth member of a family of proteins comprising the macrophage mannose receptor, the phospholipase A(...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：:A line of null mice has been produced which fails to express the transmembrane chondroitin sulfate proteoglycan NG2. Homozygous NG2 null mice do not exhibit gross phenotypic differences from wild-type mice, suggesting that detailed analyses are required to detect subtle alterations caused by the absence of NG2. Accord...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：:The ret finger protein (rfp) is a member of the B-box zinc finger gene family many of which may function in growth regulation and in the appropriate context become oncogenic. Members of this family are nuclear proteins that possess a characteristic tripartite motif consisting of the RING and B-box zinc binding domains...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
abstract：:Neuronal morphology plays an essential role in neuronal function. The establishment and maintenance of neuronal morphology is intimately linked to the actin cytoskeleton; however, the molecular mechanisms that regulate changes in neuronal morphology are poorly understood. Here we identify a novel myosin-Va (MyoVa)-int...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.050344
更新日期：2009-10-15 00:00:00
abstract：:Cell migration makes a fundamental contribution to both normal physiology and disease pathogenesis. Integrin engagement with extracellular ligands spatially controls, via the cyclical activation and deactivation of the small GTPase Rac1, the dynamic membrane protrusion and cytoskeletal reorganization events that are r...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.121988
更新日期：2013-09-15 00:00:00
abstract：:The centrosome organizes microtubules by controlling nucleation and anchoring processes. In mammalian cells, subdistal appendages of the mother centriole are major microtubule-anchoring structures of the centrosome. It is not known how newly nucleated microtubules are anchored to these appendages. We show here that ni...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02302
更新日期：2005-04-15 00:00:00
abstract：:Syntaxin 1C (STX1C), produced by alternative splicing of the stx1A gene, is a soluble syntaxin lacking a SNARE domain and a transmembrane domain. It is unclear how soluble syntaxin can control intracellular membrane trafficking. We found that STX1C affected microtubule (MT) dynamics through its tubulin-binding domain ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.081943
更新日期：2012-02-15 00:00:00
abstract：:The cell types responsible for osteolysis associated with skeletal metastasis of solid malignancies are unclear. Tumour infiltrating macrophages (TIMs) isolated from primary mammary carcinomas of C3H/Avy mice were cultured on bone slices to assess their ability to resorb bone. After 14 days in co-culture with murine m...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
abstract：:Prion diseases are fatal transmissible neurodegenerative disorders characterized by the accumulation of an abnormally folded isoform of the cellular prion protein (PrP(C)) denoted PrP(Sc). Recently, wild-type and pathogenic PrP mutants have been shown to be degraded by the endoplasmic reticulum-associated degradation ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01094
更新日期：2004-05-01 00:00:00
abstract：:SNAREs such as VAMP, SNAP-25 and syntaxin are essential for intracellular trafficking, but what are their exact molecular roles and how are their interactions with other proteins manifest? Capitalizing on the differential sensitivity of SNAREs to exogenous proteases, we quantified the selective removal of identified S...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00374
更新日期：2003-05-15 00:00:00
abstract：:Eukaryotic cell cycles are controlled by the activities of cyclin-dependent kinases (cdks). The major cdk in budding yeast, Saccharomyces cerevisiae, is Cdc28p. Activation of Cdc28p requires phosphorylation on threonine 169 and binding to a cyclin. Thr-169 is phosphorylated by the cdk-activating kinase (CAK), Cak1p, w...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-12-18 00:00:00
abstract：:The surface of the ciliate Dileptus contains locomotor and sensory domains. Each domain contains cilia of its own kind, arranged in a characteristic pattern. Transformation of a portion of the locomotor area into the sensory domain has been studied. Locomotor ciliary units in the transforming area undergo resorption o...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1983-07-01 00:00:00
abstract：:Basement membranes are complex extracellular matrices contributing to the regulation of growth, migration and differentiation of many cell types. However, little is known about the mechanisms regulating the deposition and assembly of basement membrane from its constituents. We have investigated the role of extracellul...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:The current paradigm of noncanonical NF-κB signaling suggests that the loss of TRAF2, TRAF3 or cIAP1 and cIAP2 leads to stabilization of NF-κB-inducing kinase (NIK) to activate the noncanonical pathway. Although a crucial role of RIP1 in the TNFα-induced canonical NF-κB pathway has been well established, its involveme...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.075770
更新日期：2011-02-15 00:00:00
abstract：:Notch signalling is used throughout the animal kingdom to spatially and temporally regulate cell fate, proliferation and differentiation. Its importance is reflected in the dramatic effects produced on both development and health by small variations in the strength of the Notch signal. The Down-syndrome-associated kin...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.044354
更新日期：2009-05-15 00:00:00
abstract：:Several lines of evidence indicate that increases in nuclear Ca(2+) have specific biological effects that differ from those of cytosolic Ca(2+), suggesting that they occur independently. The mechanisms involved in controlling nuclear Ca(2+) signaling are both controversial and still poorly understood. Using hypotonic ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02446
更新日期：2005-07-15 00:00:00
abstract：:During directed cell migration, the movement of the nucleus is coupled to the forward progression of the cell. The microtubule motor cytoplasmic dynein is required for both cell polarization and cell motility. Here, we investigate the mechanism by which dynein contributes to directed migration. Knockdown of dynein slo...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.033878
更新日期：2008-10-01 00:00:00
abstract：:Members of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family of proteolytic enzymes are implicated in a variety of physiological processes, such as collagen maturation, organogenesis, angiogenesis, reproduction and inflammation. Moreover, deficiency or overexpression of certain ADAMTS ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.005751
更新日期：2007-10-15 00:00:00
