Abstract：
:When they are fixed with glutaraldehyde in the presence of calcium ions, the plasma membranes of Beroe giant smooth muscle fibres display micropapillae filled with an electron-dense deposit. After freeze-fracturing of fixed tissue, the micropapillae are still present, therefore their shape and size are determined during or before glutaraldehyde fixation, and are not due to rearrangement during subsequent steps of tissue processing; intramembranous particles are seen at the periphery rather than at the top of micropapillae. In conventional stained sections, the surface of most micropapillae is surrounded by fine fibrils; when the fuzzy coat is separated from the muscle cell by a clear space, this fibrillar material becomes conspicuous and links the micropapillae to the coat. After calcium-free (EGTA) fixation, the plasma membrane is completely free of electron-dense sites but "empty' micropapillae can be seen. X-ray microanalysis of single electron-dense deposits by wavelength-dispersive spectrometry reveals a high calcium content. A weak osmiophily is suspected, but does not seem to interfere with this analysis of calcium. The highest peak-to-background ratios for calcium were obtained using the smallest aperture of the Wehnelt of the analytical microscope. In the Discussion, the micropapillae are compared to similar structures described by other authors in a variety of cell types.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Nicaise G,Hernandez-Nicaise ML,Malaval Lsubject
Has Abstractpub_date
1982-06-01 00:00:00pages
353-64eissn
0021-9533issn
1477-9137journal_volume
55pub_type
杂志文章abstract：:The semaphorin family comprises soluble and membrane-bound proteins originally identified as axonal guidance cues that function during neuronal development. Emerging evidence suggests that a subset of semaphorins, called 'immune semaphorins', function in the immune system. The class IV semaphorins Sema4D/CD100 and Sem...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
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更新日期：2003-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章,收录出版
doi：10.1242/jcs.02697
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journal_title：Journal of cell science
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更新日期：2001-02-01 00:00:00
abstract：:The requirement for a nucleolus in the expression of structural genes of nuclear viruses was examined by means of experiments in which the nucleolus or other parts of the nucleus was inactivated by a microbean of unltraviolet light. These experiments showed that the expression of such genes is not dependent on cucleol...
journal_title：Journal of cell science
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更新日期：1975-05-01 00:00:00
abstract：:Mutations in inositol polyphosphate 5-phosphatase E (INPP5E) cause the ciliopathies known as Joubert and MORM syndromes; however, the role of INPP5E in ciliary biology is not well understood. Here, we describe an interaction between INPP5E and AURKA, a centrosomal kinase that regulates mitosis and ciliary disassembly,...
journal_title：Journal of cell science
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更新日期：2015-01-15 00:00:00
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journal_title：Journal of cell science
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更新日期：2012-11-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2000-04-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.110007
更新日期：2012-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：2015-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：2015-03-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1979-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.090563
更新日期：2011-12-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.044255
更新日期：2009-05-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-02-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-02-01 00:00:00
abstract：:Notch signaling is pivotal for the morphogenesis and homeostasis of many tissues. We found that aberrant Notch activation in mouse neural-crest-derived periocular mesenchymal cells (POMCs), which contribute to the formation of corneal and eyelid stroma, results in blepharophimosis. Compound transgenic mice overexpress...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.085001
更新日期：2011-08-01 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：1997-01-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2017-07-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03361
更新日期：2007-02-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1977-08-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2014-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：2003-03-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01153
更新日期：2004-06-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1986-02-01 00:00:00
abstract：:In previous work we used immunofluorescent staining with specific antibodies to study the distribution of five desmosomal antigens in the epithelia of different vertebrate animals. We showed that all five antigens were present in all epithelia studied in human, bovine, rat, guinea pig, chick and frog (Rana pipiens) ti...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1984-03-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-02-01 00:00:00
abstract：:The scaffold attachment factor SAFB1 and its recently discovered homologue SAFB2 might provide an important link between pre-mRNA splicing, intracellular signalling and transcription. Using novel mono-specific antisera, we found endogenous SAFB2 protein has a different spatial distribution from SAFB1 within the nucleu...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.03344
更新日期：2007-01-15 00:00:00
