Abstract:
:Ferlin proteins mediate membrane-fusion events in response to Ca(2+). Myoferlin, a member of the ferlin family, is required for normal muscle development, during which it mediates myoblast fusion. We isolated both damaged and intact myofibers from a mouse model of muscular dystrophy using laser-capture microdissection and found that the levels of myoferlin mRNA and protein were increased in damaged myofibers. To better define the components of the muscle-injury response, we identified a discreet 1543-bp fragment of the myoferlin promoter, containing multiple NFAT-binding sites, and found that this was sufficient to drive high-level myoferlin expression in cells and in vivo. This promoter recapitulated normal myoferlin expression in that it was downregulated in healthy myofibers and was upregulated in response to myofiber damage. Transgenic mice expressing GFP under the control of the myoferlin promoter were generated and GFP expression in this model was used to track muscle damage in vivo after muscle injury and in muscle disease. Myoferlin modulates the response to muscle injury through its activity in both myoblasts and mature myofibers.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Demonbreun AR,Lapidos KA,Heretis K,Levin S,Dale R,Pytel P,Svensson EC,McNally EMdoi
10.1242/jcs.065375subject
Has Abstractpub_date
2010-07-15 00:00:00pages
2413-22issue
Pt 14eissn
0021-9533issn
1477-9137pii
jcs.065375journal_volume
123pub_type
杂志文章abstract::Mastigonemes are hair-like projections on the flagella of various kinds of lower eukaryotes. We obtained a monoclonal antibody (mAb-MAST1) to mastigonemes of Chlamydomonas reinhardtii, and found that it reacts with a single flagellar glycoprotein of about 230 kDa. Interestingly, immunofluorescence microscopy demonstra...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::In order to replicate, most pathogens need to enter their target cells. Many viruses enter the host cell through an endocytic pathway and hijack endosomes for their journey towards sites of replication. For delivery of their genome to the host cell cytoplasm and to avoid degradation, viruses have to escape this endoso...
journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.216259
更新日期:2018-08-03 00:00:00
abstract::Although the long-term effects of all-trans retinoic acid (RA) on neuronal growth and differentiation have been intensively studied, nothing is known about its effect on synaptic transmission. Here we show that RA rapidly and specifically enhances the spontaneous acetylcholine release at developing neuromuscular synap...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01153
更新日期:2004-06-15 00:00:00
abstract::Feedback mechanisms preventing M phase occurrence before S phase completion are assumed to depend on inhibition of cyclin B-cdc2 kinase activation by unreplicated DNA. In sea urchin, fertilization stimulates protein synthesis and releases eggs from G1 arrest. We found that in the one-cell sea urchin embryo cyclin B-cd...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1995-07-01 00:00:00
abstract::Glomerular mesangial cells (MCs) are central to the pathogenesis of progressive glomeruli-associated renal diseases. However, molecular mechanisms underlying changes in MC functions still remain poorly understood. Here, we show that in MCs, the urokinase-type plasminogen activator (uPA) induces, via its specific recep...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02409
更新日期:2005-06-15 00:00:00
abstract::We have used the H-7w subclone of a Chinese hamster ovary cell line (K1) to investigate the role of cell surface architecture (specifically microvilli, blebs, and sheets) in determining the relative agglutinability of a cell line with Con A. Our evidence clearly demonstrates that no specific, immediately recognizable ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1978-12-01 00:00:00
abstract::The attachment of polymorphonuclear leukocytes (PMN) to the endothelial lining of blood vessels is an important initial event in the acute inflammatory reaction. Experiments were carried out to examine some of the parameters of this adhesive interaction and the extent to which the substrate (endothelium) plays a role ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1980-10-01 00:00:00
abstract::HC11 is a normal mouse mammary epithelial cell line that requires certain growth factors, such as EGF or bFGF, to respond optimally to lactogenic hormones and produce the differentiation marker beta-casein. Growth in insulin (Ins) or PDGF does not produce cells competent to respond to lactogenic hormones. Here we show...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1994-04-01 00:00:00
abstract::Formins constitute a family of eukaryotic proteins that are considered to function as a cytoskeleton organizer to regulate morphogenesis, cell polarity and cytokinesis. Fhos is a recently identified mammalian formin, which contains the conserved domains FH (formin homology) 1 and FH2 in the middle region and the Dia-a...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00769
更新日期:2003-11-15 00:00:00
abstract::Cell chemotaxis, such as migration of fibroblasts towards growth factors during development and wound healing, requires precise spatial coordination of signalling events. Phosphoinositides and signalling enzymes involved in their generation and hydrolysis have been implicated in regulation of chemotaxis; however, the ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.110007
更新日期:2012-12-01 00:00:00
abstract::The myosin regulatory light chain (RLC) of Dictyostelium discoideum is phosphorylated at a single serine site in response to chemoattractant. To investigate the role of the phosphorylation of RLC in both motility and chemotaxis, mutants were generated in which the single phosphorylatable serine was replaced with a non...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2002-04-15 00:00:00
abstract::When migrating mesenchymal cells collide, they exhibit a 'contact inhibition of locomotion' response that results in reversal of their front-rear polarity by extension of a new leading edge, which enables their migration away from the opposing contacted cell. The critical cytoskeletal rearrangements underpinning these...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.087965
更新日期:2011-08-01 00:00:00
abstract::Key factors in the adenosine 3',5'-cyclic monophosphate (cyclic AMP) metabolic pathway are two enzymes responsible for its generation and degradation, namely, adenylate cyclase (AC) and phosphodiesterase (PDE). In LD: 12,12 (12 h light, 12 h dark), these enzymes were found to undergo bimodal, circadian variation of ac...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1991-10-01 00:00:00
abstract::The formation of cytoplasmic stress granules and the innate immune response are two distinct cellular stress responses. Our study investigated the involvement of four innate immune proteins - retinoic-acid-inducible gene I (RIG-I, also known as DDX58), melanoma differentiation-associated gene 5 (MDA5, also known as IF...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.139626
更新日期:2014-06-01 00:00:00
abstract::Kalirin, one of the few Rho guanine nucleotide exchange factors (GEFs) that contains spectrin-like repeats, plays a critical role in axon extension and maintenance of dendritic spines. PC12 cells were used to determine whether Cdk5, a critical participant in both processes, regulates the action of Kalirin. Expression ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.016089
更新日期:2008-08-01 00:00:00
abstract::Cilia and flagella are conserved eukaryotic organelles important for motility and sensory. The RanGTPase, best known for nucleocytoplasmic transport functions, may also play a role in protein trafficking into the specialized flagellar/ciliary compartments, although the regulatory mechanisms controlling Ran activity at...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.148015
更新日期:2014-11-15 00:00:00
abstract::In the central nervous system, oligodendrocytes synthesize vast amounts of myelin, a multilamellar membrane wrapped around axons that dramatically enhances nerve transmission. A complex apparatus appears to coordinate trafficking of proteins and lipids during myelin synthesis, but the molecular interactions involved a...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02785
更新日期:2006-03-01 00:00:00
abstract::A temperature-sensitive mutant tsBN63 cell line was isolated by the fluorodeoxyuridine method from the BHK21/13 cell line after mutagenesis with nitrosoguanidine. When cultures of tsBN63 cells growing asynchronously at 33.5 degrees C were shifted to 39.5 degrees C, a nonpermissive temperature, the ability for protein ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1991-09-01 00:00:00
abstract::Increasing evidence indicates that cellular uptake of several molecules can occur independently of functional dynamin, but the molecular players that regulate dynamin-independent endocytosis and the subsequent trafficking steps are still largely unknown. A survival-based short-hairpin (sh) RNA screen using a cell line...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.071712
更新日期:2010-12-15 00:00:00
abstract::Casein kinase 1 (CK1) plays central roles in various signal transduction pathways and performs many cellular activities. For many years CK1 was thought to act independently of modulatory subunits and in a constitutive manner. Recently, DEAD box RNA helicases, in particular DEAD box RNA helicase 3 X-linked (DDX3X), wer...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.207316
更新日期:2018-01-04 00:00:00
abstract::The conversion of the cellular prion protein (PrPc) into a pathogenic isoform (PrP(Sc)) is one of the underlying events in the pathogenesis of the fatal transmissible spongiform encephalopathies (TSEs). Numerous compounds have been described to inhibit prion replication and PrP(Sc) accumulation in cell culture. Among ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02609
更新日期:2005-11-01 00:00:00
abstract::The 43 kDa inositol polyphosphate 5-phosphatase (5-phosphatase) hydrolyses the signalling molecules inositol 1,4,5-trisphosphate (Ins(1,4,5)P(3)) and inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4, 5)P(4)) in a signal-terminating reaction. We have utilised cell lines that stably underexpress the 43 kDa 5-phosphatase, a...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract::Poly(ADP-ribosyl)ation is a post-translational modification of proteins involved in the regulation of chromatin structure, DNA metabolism, cell division and cell death. Through the hydrolysis of poly(ADP-ribose) (PAR), Poly(ADP-ribose) glycohydrolase (PARG) has a crucial role in the control of life-and-death balance f...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.039115
更新日期:2009-06-15 00:00:00
abstract::Macroautophagy is a dynamic process whereby portions of the cytosol are encapsulated in double-membrane vesicles and delivered to the lysosome for degradation. Phosphatidylinositol-3-phosphate (PtdIns3P) is concentrated on autophagic vesicles and recruits effector proteins that are crucial for this process. The produc...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.080341
更新日期:2011-03-01 00:00:00
abstract::Endothelial integrity relies on a mechanical crosstalk between intercellular and cell-matrix interactions. This crosstalk is compromised in hemorrhagic vascular lesions of patients carrying loss-of-function mutations in cerebral cavernous malformation (CCM) genes. RhoA/ROCK-dependent cytoskeletal remodeling is central...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.216093
更新日期:2018-08-13 00:00:00
abstract::Here, we identify a role for the matrilin-2 (Matn2) extracellular matrix protein in controlling the early stages of myogenic differentiation. We observed Matn2 deposition around proliferating, differentiating and fusing myoblasts in culture and during muscle regeneration in vivo. Silencing of Matn2 delayed the express...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.141556
更新日期:2014-08-01 00:00:00
abstract::When a Euglena gracilis in a Ca2+-containing medium is impaled with a microelectrode, the flagellum is instantly ejected. In a Ca2+-free medium to which 1 mM EGTA has been added, the flagellum remains attached to the organism, but it loses activity upon impalement. Externally added ATP at a concentration of 10 mM will...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1977-02-01 00:00:00
abstract::SNARE [soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) receptor] proteins control the membrane-fusion events of eukaryotic membrane-trafficking pathways. Specific vesicular and target SNAREs operate in specific trafficking routes, but the degree of specificity of SNARE functions is still elus...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.007948
更新日期:2007-09-15 00:00:00
abstract::Arf GTPases regulate both the morphological and protein sorting events that are essential for membrane trafficking. Guanine nucleotide exchange factors (GEFs) specific for Arf proteins determine when and where Arf GTPases will be activated in cells. The yeast Gea2p Arf GEF is a member of an evolutionarily conserved fa...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00896
更新日期:2004-02-15 00:00:00
abstract::Many pathways dysregulated in prostate cancer are also involved in epithelial differentiation. To better understand prostate tumor initiation, we sought to investigate specific genes and mechanisms required for normal basal to luminal cell differentiation. Utilizing human prostate basal epithelial cells and an in vitr...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.197152
更新日期:2017-06-01 00:00:00