Spatial regulation of RhoC activity defines protrusion formation in migrating cells.

abstract：:We report the first RNAi-induced phenotypes in mammalian cultured cells using RNA interference mediated by duplexes of 21-nt RNAs. The 21 gene products studied have different functions and subcellular localizations. Knockdown experiments monitored by immunofluorescence and immunoblotting show that even major cellular ...

journal_title：Journal of cell science

authors： Harborth J,Elbashir SM,Bechert K,Tuschl T,Weber K

更新日期：2001-12-01 00:00:00

abstract：:Dynamin-related GTPase proteins (DRPs) are main players in membrane remodelling. Conserved DRPs called mitofusins (Mfn1/Mfn2/Fzo1) mediate the fusion of mitochondrial outer membranes (OM). OM fusion depends on self-assembly and GTPase activity of mitofusins as well as on two other proteins, Ugo1 and Mdm30. Here, we de...

journal_title：Journal of cell science

authors： Anton F,Fres JM,Schauss A,Pinson B,Praefcke GJ,Langer T,Escobar-Henriques M

更新日期：2011-04-01 00:00:00

abstract：:Natural killer (NK) cells eliminate abnormal cells through the release of cytolytic granule contents. In this process, NK cells must adhere to target cells through integrin-mediated adhesion, which is highly dependent on the generation of F-actin. Ena/VASP-like (EVL) is an actin regulatory protein previously shown to ...

journal_title：Journal of cell science

authors： Wilton KM,Overlee BL,Billadeau DD

更新日期：2019-07-18 00:00:00

abstract：:Although the entry of DNA into the nucleus is a crucial step of non-viral gene delivery, fundamental features of this transport process have remained unexplored. This study analyzed the effect of linear double stranded DNA size on its passive diffusion, its active transport and its NLS-assisted transport. The size lim...

journal_title：Journal of cell science

authors： Ludtke JJ,Zhang G,Sebestyén MG,Wolff JA

更新日期：1999-06-01 00:00:00

abstract：:Analysis by double-label indirect immunofluorescence of PtK1 and HeLa cells had previously demonstrated that prosome* antigens form networks that superimpose on those of the intermediate filaments of the cytokeratin type. We show here that in PtK1 cells various prosomal antigens also reside to a variable extent on int...

journal_title：Journal of cell science

authors： Olink-Coux M,Arcangeletti C,Pinardi F,Minisini R,Huesca M,Chezzi C,Scherrer K

更新日期：1994-03-01 00:00:00

abstract：:Recently, we have presented evidence that proliferating blood vessels produce and deposit fibronectin in situ during the angiogenesis of wound repair. This report extends these observations by demonstrating that human endothelial cells from both large and small vessels depend on fibronectin for their adherence in vitr...

journal_title：Journal of cell science

authors： Clark RA,Folkvord JM,Nielsen LD

更新日期：1986-06-01 00:00:00

abstract：:Megalin is a receptor expressed by embryonic epithelia that mediates endocytosis of numerous ligands, including sonic hedgehog (Shh) and retinol, the precursor to retinoic acid (RA). The importance of Shh and RA signaling in neurodevelopment, combined with the fact that megalin-deficient mice show profound neurodevelo...

journal_title：Journal of cell science

authors： McCarthy RA,Argraves WS

更新日期：2003-03-15 00:00:00

abstract：:Sorting nexins are a large family of phox-homology-domain-containing proteins that have been implicated in the control of endosomal sorting. Sorting nexin-1 is a component of the mammalian retromer complex that regulates retrieval of the cation-independent mannose 6-phosphate receptor from endosomes to the trans-Golgi...

journal_title：Journal of cell science

authors： Carlton JG,Bujny MV,Peter BJ,Oorschot VM,Rutherford A,Arkell RS,Klumperman J,McMahon HT,Cullen PJ

更新日期：2005-10-01 00:00:00

abstract：:In culture, cell confluence generates signals that commit actively growing keratinocytes to exit the cell cycle and differentiate to form a stratified epithelium. Using a comparative proteomic approach, we studied this 'confluence switch' and identified a new pathway triggered by cell confluence that regulates basemen...

journal_title：Journal of cell science

authors： Botta A,Delteil F,Mettouchi A,Vieira A,Estrach S,Négroni L,Stefani C,Lemichez E,Meneguzzi G,Gagnoux-Palacios L

更新日期：2012-09-15 00:00:00

abstract：:The Cep63-Cep152 complex located at the mother centriole recruits Plk4 to initiate centriole biogenesis. How the complex is targeted to mother centrioles, however, is unclear. In this study, we show that Cep57 and its paralog, Cep57l1, colocalize with Cep63 and Cep152 at the proximal end of mother centrioles in both c...

journal_title：Journal of cell science

authors： Zhao H,Yang S,Chen Q,Duan X,Li G,Huang Q,Zhu X,Yan X

更新日期：2020-07-03 00:00:00

abstract：:Transcription factor IIH (TFIIH) participates in transcription, nucleotide excision repair and the control of the cell cycle. In the present study, we demonstrate that the Dmp52 subunit of TFIIH in Drosophila physically interacts with the fly p53 homologue, Dp53. The depletion of Dmp52 in the wing disc generates chrom...

journal_title：Journal of cell science

authors： Villicaña C,Cruz G,Zurita M

更新日期：2013-06-01 00:00:00

abstract：:Long noncoding RNAs (lncRNAs) have emerged as important regulators of cancer progression. Abnormal sialylation leads to renal cell carcinoma (RCC) malignancy. However, the mechanism by which the lncRNA maternally expressed gene 3 (MEG3) mediates RCC progression by regulating ST3Gal1 transcription and EGFR sialylation ...

journal_title：Journal of cell science

authors： Gong A,Zhao X,Pan Y,Qi Y,Li S,Huang Y,Guo Y,Qi X,Zheng W,Jia L

更新日期：2020-08-25 00:00:00

abstract：:Rac1 has a crucial role in epidermal growth factor (EGF)-induced membrane ruffling, lamellipodial protrusion, and cell migration. Several guanine nucleotide exchange factors (GEFs) including Sos1, Sos2, Tiam1 and Vav2 have been shown to transduce the growth signal from the EGF receptor to Rac1. To clarify the role of ...

journal_title：Journal of cell science

authors： Itoh RE,Kiyokawa E,Aoki K,Nishioka T,Akiyama T,Matsuda M

更新日期：2008-08-15 00:00:00

abstract：:Cyclic AMP-dependent protein kinase (cAPK) is the intracellular mediator of signal transduction events involving the adenylate cyclase-cyclic AMP system. A monoclonal antibody (MAb BB1) to the type II regulatory subunit (RII) of cAPK was used in a post-embedding immunogold-labeling procedure to determine the ultrastru...

journal_title：Journal of cell science

authors： Hand AR,Mednieks MI

更新日期：1989-08-01 00:00:00

abstract：:We have shown previously, by fluorescent antibody staining, that desmosomal antigens are widely distributed in the tissues of vertebrate animals. Furthermore, we have demonstrated mutual desmosome formation between cells derived from man, cow, dog, chicken and frog. In this paper we have studied the components of desm...

journal_title：Journal of cell science

authors： Suhrbier A,Garrod D

更新日期：1986-03-01 00:00:00

abstract：:Endocytosis is a regulated physiological process by which membrane receptors and their extracellular ligands are internalized. After internalization, they enter the endosomal trafficking pathway for sorting and processing. Amphiphysins consist of a family of proteins conserved throughout evolution that are crucial ele...

journal_title：Journal of cell science

authors： Leprince C,Le Scolan E,Meunier B,Fraisier V,Brandon N,De Gunzburg J,Camonis J

更新日期：2003-05-15 00:00:00

abstract：:Mammalian, or mechanistic, target of rapamycin complex 2 (mTORC2) regulates a variety of vital cellular processes, and its aberrant functioning is often associated with various diseases. Rictor is a peculiar and distinguishing mTORC2 component playing a pivotal role in controlling its assembly and activity. Among exta...

journal_title：Journal of cell science

authors： Pergolizzi B,Panuzzo C,Ali MS,Lo Iacono M,Levra Levron C,Ponzone L,Prelli M,Cilloni D,Calautti E,Bozzaro S,Bracco E

更新日期：2019-11-14 00:00:00

abstract：:Endocytosis and recycling of membrane proteins are key processes for nutrient uptake, receptor signaling and synaptic transmission. Different steps in these fission and fusion cycles have been proposed to be regulated by physiological changes in plasma membrane (PM) phosphatidylinositol (4,5)-bisphosphate [PtdIns(4,5)...

journal_title：Journal of cell science

authors： Abe N,Inoue T,Galvez T,Klein L,Meyer T

更新日期：2008-05-01 00:00:00

abstract：:The water pipes of elongating plant organs are the result of programmed cell death and are formed by the walls of dead and empty protoxylem elements. These protoxylem elements are passively elongated many times by the surrounding tissue before they are replaced and collapse. Well-known adaptations for this unique task...

journal_title：Journal of cell science

authors： Ryser U,Schorderet M,Guyot R,Keller B

更新日期：2004-03-01 00:00:00

abstract：:A universal feature of mitosis is that all chromosomes become aligned at the spindle equator--the halfway point between the two spindle poles--prior to anaphase onset. This migratory event is called congression, and is powered by centromere-bound protein machines called kinetochores. This Commentary aims to document r...

journal_title：Journal of cell science

authors： Auckland P,McAinsh AD

更新日期：2015-09-15 00:00:00

abstract：:Annexin A2 (AnxA2) is a multi-functional and -compartmental protein whose subcellular localisation and functions are tightly regulated by its post-translational modifications. AnxA2 and its Tyr23-phosphorylated form (pTyr23AnxA2) are involved in malignant cell transformation, metastasis and angiogenesis. Here, we show...

journal_title：Journal of cell science

authors： Grindheim AK,Hollås H,Raddum AM,Saraste J,Vedeler A

更新日期：2016-01-15 00:00:00

abstract：:Compartmentalization of membrane transport and signaling processes is of pivotal importance to eukaryotic cell function. While plasma membrane compartmentalization and dynamics are well known to depend on the scaffolding function of septin GTPases, the roles of septins at intracellular membranes have remained largely ...

journal_title：Journal of cell science

authors： Song K,Gras C,Capin G,Gimber N,Lehmann M,Mohd S,Puchkov D,Rödiger M,Wilhelmi I,Daumke O,Schmoranzer J,Schürmann A,Krauss M

更新日期：2019-02-01 00:00:00

abstract：:In chicken oocytes, proteins of the K/J family or their analogs, such as are known to be involved in mRNA processing in humans, are closely associated with nascent C-rich RNA transcripts on the loops of lampbrush chromosomes. Using labelled single stranded nucleotide probes and an antibody to protein K, these C-rich t...

journal_title：Journal of cell science

authors： Solovei I,Macgregor H,Gaginskaya E

更新日期：1995-04-01 00:00:00

abstract：:Nopp44/46 is a phosphoprotein of the protozoan parasite Trypanosoma brucei that is localized to the nucleolus. Based on the primary sequence, Nopp44/46 appears to be a protein composed of distinct domains. This communication describes the relationship of these domains to the known functional interactions of the molecu...

journal_title：Journal of cell science

authors： Das A,Park JH,Hagen CB,Parsons M

更新日期：1998-09-01 00:00:00

abstract：:Myosin VI is an actin-based retrograde motor protein that plays a crucial role in both endocytic and secretory membrane trafficking pathways. Myosin VI's targeting to and function in these intracellular pathways is mediated by a number of specific binding partners. In this paper we have identified a new myosin-VI-bind...

journal_title：Journal of cell science

authors： Chibalina MV,Seaman MN,Miller CC,Kendrick-Jones J,Buss F

更新日期：2007-12-15 00:00:00

abstract：:The function of microtubules depends on their arrangement into highly ordered arrays. Spatio-temporal control over the formation of new microtubules and regulation of their properties are central to the organization of these arrays. The nucleation of new microtubules requires γ-tubulin, an essential protein that assem...

journal_title：Journal of cell science

authors： Teixidó-Travesa N,Roig J,Lüders J

更新日期：2012-10-01 00:00:00

abstract：:The 16 kDa proteolipid (subunit c) of the eukaryotic vacuolar H(+)-ATPase (V-ATPase) is closely related to the ductin polypeptide that forms the connexon channel of gap junctions in the crustacean Nephrops norvegicus. Here we show that the major protein component of Manduca sexta gap junction preparations is a 16 kDa ...

journal_title：Journal of cell science

authors： Finbow ME,Goodwin SF,Meagher L,Lane NJ,Keen J,Findlay JB,Kaiser K

更新日期：1994-07-01 00:00:00

abstract：:ARF-like proteins (ARLs) are distinct group of members of the ARF family of Ras-related GTPases. Although ARLs are very similar in primary structure to ARFs, their functions remain unclear. We cloned mouse (m) and human (h) ARL5 cDNAs to characterize the protein products and their molecular properties. mARL5 mRNA was ...

journal_title：Journal of cell science

authors： Lin CY,Li CC,Huang PH,Lee FJ

更新日期：2002-12-01 00:00:00

abstract：:Tumour necrosis factor receptors (TNF-Rs) and their ligands, tumour necrosis factors, are highly conserved proteins described in all metazoan phyla. They function as inducers of extrinsic apoptotic signalling and facilitate inflammation, differentiation and cell survival. TNF-Rs use distinct adaptor molecules to activ...

journal_title：Journal of cell science

authors： Steichele M,Sauermann LS,König AC,Hauck S,Böttger A

更新日期：2021-01-25 00:00:00

abstract：:We have recently shown that leukotriene D(4) (LTD(4)) increases cell survival in intestinal epithelial cells. Here we report and explore the complementary finding that LTD(4) also enhances proliferation in these cells. This proliferative response was approximately half of that induced by epidermal growth factor (EGF) ...

journal_title：Journal of cell science

authors： Paruchuri S,Hallberg B,Juhas M,Larsson C,Sjölander A

更新日期：2002-05-01 00:00:00