Dissecting the role of PtdIns(4,5)P2 in endocytosis and recycling of the transferrin receptor.

abstract：:Immediately following embryonic cleavage, the cells of Astrephomene have four equal-sized basal bodies, two of which are connected by a striated distal fibre and two striated proximal fibres. The four microtubular rootlets, which alternate between having 3/1 and 2 members, are arranged cruciately. The two basal bodies...

journal_title：Journal of cell science

authors： Hoops HJ,Floyd GL

更新日期：1983-09-01 00:00:00

abstract：:We have discovered a novel mitosis-specific human autoantigen that arises at the centromeres of prophase chromosomes, but ultimately participates in formation of an organelle that bisects the cell at late anaphase and during telophase. The organelle, discernible as a three-dimensional disc by confocal microscopy, enco...

journal_title：Journal of cell science

authors： Andreassen PR,Palmer DK,Wener MH,Margolis RL

更新日期：1991-07-01 00:00:00

abstract：:The position of the nucleus is regulated in different developmental stages and cellular events. During polarization, the nucleus moves away from the future leading edge and this movement is required for proper cell migration. Nuclear movement requires the LINC complex components nesprin-2G and SUN2, which form transme...

journal_title：Journal of cell science

authors： Borrego-Pinto J,Jegou T,Osorio DS,Auradé F,Gorjánácz M,Koch B,Mattaj IW,Gomes ER

更新日期：2012-03-01 00:00:00

abstract：:The actin-dependent sensory and response elements of stromal cells that are involved in mechanical signal transduction are poorly understood. To study mechanotransduction we have described previously a collagen-magnetic bead model in which application of well-defined forces to integrins induces an immediate (< 1 secon...

journal_title：Journal of cell science

authors： Glogauer M,Arora P,Yao G,Sokholov I,Ferrier J,McCulloch CA

更新日期：1997-01-01 00:00:00

abstract：:The sensory functions of cilia are dependent on the enrichment of cilium-resident proteins. Although it is known that ciliary targeting signals (CTSs) specifically target ciliary proteins to cilia, it is still unclear how CTSs facilitate the entry and retention of cilium-resident proteins at the molecular level. We fo...

journal_title：Journal of cell science

authors： Madugula V,Lu L

更新日期：2016-10-15 00:00:00

abstract：:Successful implantation requires the synchronization of viable embryonic development with endometrial receptivity. The mechanisms allowing for the initiation of crosstalk between the embryo and the endometrium remain elusive; however, recent studies have revealed that there are alterations in endometrial microRNAs (mi...

journal_title：Journal of cell science

authors： Kang YJ,Lees M,Matthews LC,Kimber SJ,Forbes K,Aplin JD

更新日期：2015-02-15 00:00:00

abstract：:An electron-microscopic study of various fractions taken during a newly developed method for preparation of brush border vesicles from rat duodenum, has shown that vesiculation of microvilli can occur in situ on the brush border membrane. This mode of formation ensures that the vesicle membranes are oriented as in viv...

journal_title：Journal of cell science

authors： Hearn PR,Russell RG,Farmer J

更新日期：1981-02-01 00:00:00

abstract：:The weak spindle integrity checkpoint in Drosophila spermatocytes has revealed a novel function of the gamma-tubulin ring complex (gammaTuRC) in maintaining spindle bipolarity throughout meiosis. Bipolar and bi-astral spindles could form in Drosophila mutants for dd4, the gene encoding the 91 kDa subunit of gammaTuRC....

journal_title：Journal of cell science

authors： Barbosa V,Gatt M,Rebollo E,Gonzalez C,Glover DM

更新日期：2003-03-01 00:00:00

abstract：:A high molecular weight tight junction-associated protein, ZO-1, has been demonstrated in liver (hepatocytes) and in both epithelium and endothelium. We carried out studies to examine the presence of the protein in vascular endothelial cell cultures and several other types of cultured cells, and the relationship betwe...

journal_title：Journal of cell science

authors： Li CX,Poznansky MJ

更新日期：1990-10-01 00:00:00

abstract：:When cultured human lymphocytes (IM-9) are exposed to 10(-6) M procine insulin for 6 h, washed, and incubated with 125I-insulin, the ability of the cell to bind the labelled hormone is reduced by a mean of 78%. Under these experimental conditions that induce insulin-receptor loss in this cell there is a mean 95% incre...

journal_title：Journal of cell science

authors： Gordon P,Carpentier JL,Van Obberghen E,Barazzone P,Roth J,Orci L

更新日期：1979-10-01 00:00:00

abstract：:The mammalian retromer complex is a multi-protein complex that regulates retrograde transport of the cation-independent mannose 6-phosphate receptor (CI-MPR) from early endosomes to the trans Golgi network (TGN). It consists of two subcomplexes: a membrane-bound coat comprising sorting nexin-1 (SNX1) and possibly sort...

journal_title：Journal of cell science

authors： Bujny MV,Popoff V,Johannes L,Cullen PJ

更新日期：2007-06-15 00:00:00

abstract：:When migrating mesenchymal cells collide, they exhibit a 'contact inhibition of locomotion' response that results in reversal of their front-rear polarity by extension of a new leading edge, which enables their migration away from the opposing contacted cell. The critical cytoskeletal rearrangements underpinning these...

journal_title：Journal of cell science

authors： Kadir S,Astin JW,Tahtamouni L,Martin P,Nobes CD

更新日期：2011-08-01 00:00:00

abstract：:The monoclonal antibody Bx63 recognizes a centrosomal antigen of Drosophila melanogaster by indirect immunofluorescence and identifies two proteins, with apparent molecular weights of 185 x 10(3) and 66 x 10(3), on Western blots. We have used this antibody to isolate five clones (lambda cs1, -2, -3, -4 and lambda j63)...

journal_title：Journal of cell science

authors： Whitfield WG,Millar SE,Saumweber H,Frasch M,Glover DM

更新日期：1988-04-01 00:00:00

abstract：:The effect of glycosaminoglycans on the invasion of choroid fibroblasts into type I collagen gels was studied. Both hyaluronate and chondroitin sulphate, when incorporated into the gel, facilitated invasion of the collagen matrix, although hyaluronate was considerably more effective. Hyaluronate-induced fibroblast inv...

journal_title：Journal of cell science

authors： Docherty R,Forrester JV,Lackie JM,Gregory DW

更新日期：1989-02-01 00:00:00

abstract：:Anionic phospholipids PI(4,5)P2 and phosphatidylserine (PS) are enriched in the cytosolic leaflet of the plasma membrane where endocytic sites form. In this study, we investigated the roles of PI(4,5)P2 and PS in clathrin-mediated endocytosis (CME) site initiation and vesicle formation in Saccharomyces cerevisiae. Liv...

journal_title：Journal of cell science

authors： Sun Y,Drubin DG

更新日期：2012-12-15 00:00:00

abstract：:The process of cell migration is initiated by protrusion at the leading edge of the cell, the formation of peripheral adhesions, the exertion of force on these adhesions, and finally the release of the adhesions at the rear of the cell. Focal adhesion kinase (FAK) is intimately involved in the regulation of this proce...

journal_title：Journal of cell science

authors： Tilghman RW,Slack-Davis JK,Sergina N,Martin KH,Iwanicki M,Hershey ED,Beggs HE,Reichardt LF,Parsons JT

更新日期：2005-06-15 00:00:00

abstract：:The functions of polycomb products extend beyond their well-known activity as transcriptional regulators to include genome duplication processes. Polycomb activities during DNA replication and DNA damage repair are unclear, particularly without induced replicative stress. We have used a cellular model of conditionally...

journal_title：Journal of cell science

authors： Bravo M,Nicolini F,Starowicz K,Barroso S,Calés C,Aguilera A,Vidal M

更新日期：2015-10-01 00:00:00

abstract：:Prions are proteins that access self-templating amyloid forms, which confer phenotypic changes that can spread from individual to individual within or between species. These infectious phenotypes can be beneficial, as with yeast prions, or deleterious, as with mammalian prions that transmit spongiform encephalopathies...

journal_title：Journal of cell science

authors： Cushman M,Johnson BS,King OD,Gitler AD,Shorter J

更新日期：2010-04-15 00:00:00

abstract：:Melanotransferrin (p97) is an iron-binding membrane glycoprotein with 40% homology to transferrin and lactoferrin. It was first identified on the basis of its high level of expression in melanoma cells, as compared to normal melanocytes. It is also present in many cultured cell types. In normal tissues, p97 is express...

journal_title：Journal of cell science

authors： Alemany R,Vilá MR,Francí C,Egea G,Real FX,Thomson TM

更新日期：1993-04-01 00:00:00

abstract：:In order to produce proteins essential for their propagation, many pathogenic human viruses, including SARS-CoV-2 the causative agent of COVID-19 respiratory disease, commandeer host biosynthetic machineries and mechanisms. Three major structural proteins, the spike, envelope and membrane proteins, are amongst several...

journal_title：Journal of cell science

authors： O'Keefe S,Roboti P,Duah KB,Zong G,Schneider H,Shi WQ,High S

更新日期：2021-01-19 00:00:00

abstract：:When a Euglena gracilis in a Ca2+-containing medium is impaled with a microelectrode, the flagellum is instantly ejected. In a Ca2+-free medium to which 1 mM EGTA has been added, the flagellum remains attached to the organism, but it loses activity upon impalement. Externally added ATP at a concentration of 10 mM will...

journal_title：Journal of cell science

authors： Nichols KM,Rikmenspoel R

更新日期：1977-02-01 00:00:00

abstract：:The molecular chaperone Hsp90 has been found ubiquitously as a predominantly cytoplasmic dimer. By interacting with cytoplasmic or nuclear proteins such as pp60v-src or steroid receptors, Hsp90 helps its targets to become competent for full biological activity. Mutational deletion analysis of some properties of chicke...

journal_title：Journal of cell science

authors： Meng X,Devin J,Sullivan WP,Toft D,Baulieu EE,Catelli MG

更新日期：1996-07-01 00:00:00

abstract：:Re-epithelialization after skin wounding requires both migration and hyperproliferation of keratinocytes. Laminin-332 is deposited during migration over the provisional matrix. To investigate the function of the laminin-332 binding integrin alpha3beta1 in wound re-epithelialization, we generated Itga3flox/flox; K14-Cr...

journal_title：Journal of cell science

authors： Margadant C,Raymond K,Kreft M,Sachs N,Janssen H,Sonnenberg A

更新日期：2009-01-15 00:00:00

abstract：:Recent studies have shown that Src-family kinases (SFKs) play an important role in mediating integrin signalling, and the beta3 subunit of alphaIIbbeta3 integrin has been shown to interact with multiple SFK members. Here, we analyzed the interactions and functional consequences of Fyn and Src binding to alphaIIbbeta3....

journal_title：Journal of cell science

authors： Reddy KB,Smith DM,Plow EF

更新日期：2008-05-15 00:00:00

abstract：:It is becoming clear that mechanical stimuli are crucial factors in regulating the biology of the cell, but the short-term structural response of a cell to mechanical forces remains relatively poorly understood. We mechanically stimulated cells transiently expressing actin-EGFP with controlled forces (0-20 nN) in orde...

journal_title：Journal of cell science

authors： Guolla L,Bertrand M,Haase K,Pelling AE

更新日期：2012-02-01 00:00:00

abstract：:In epithelial cells integrins are segregated on discrete domains of the plasma membrane. Redistribution may also occur during migration or differentiation. However, little is known about the mechanisms that control such redistribution. Receptor internalization may be a part of one such mechanism. We developed a quanti...

journal_title：Journal of cell science

authors： Gaietta G,Redelmeier TE,Jackson MR,Tamura RN,Quaranta V

更新日期：1994-12-01 00:00:00

abstract：:We report the first direct analysis of the endogenous β-catenin phosphorylation activity in colon cancer SW480 cells. By comparing parental SW480 cells that harbor a typical truncated adenomatous polyposis coli (APC) form, cells expressing full-length APC and APC-depleted cells, we provide the formal demonstration tha...

journal_title：Journal of cell science

authors： Wang L,Liu X,Gusev E,Wang C,Fagotto F

更新日期：2014-04-15 00:00:00

abstract：:A transformed variant derived as a clone from normal 3T3 cells infected with simian virus 40 (SV40) has been found to possess a phenotype intermediate between that of normal cells and that characteristic of the transformed state, yet cells of the variant still test positively for the SV40-specific nuclear T-antigen. T...

journal_title：Journal of cell science

authors： Thompson JE,Elligsen JD,Frey HE

更新日期：1975-08-01 00:00:00

abstract：:Prostaglandins E1, E2, and F2alpha (PGE1, PGE2, and PGF2alpha) were shown to inhibit the growth of mouse leukaemia lymphoblasts L5178Y in culture. The effects of PGE1 and PGE2 were greater than that of PGF2alpha. PGE1 and PGE2, at the concentration of 100 mug per ml showed significant inhibitory effects on the rates o...

journal_title：Journal of cell science

authors： Yang TJ,Dale JB,Machanoff R

更新日期：1976-01-01 00:00:00

abstract：:The canonical Wnt/β-catenin signaling pathway regulates cell proliferation in development and adult tissue homeostasis. Dysregulated signaling contributes to human diseases, in particular cancer. Growing evidence suggests a role for clathrin and/or endocytosis in the regulation of this pathway, but conflicting results...

journal_title：Journal of cell science

authors： Munthe E,Raiborg C,Stenmark H,Wenzel EM

更新日期：2020-07-09 00:00:00