Abstract：
:Targeting of the NG2 proteoglycan to cellular retraction fibers was studied by expressing mutant NG2 molecules lacking specific structural elements of the proteoglycan. Both the cytoplasmic domain and the chondroitin sulfate chain of NG2 appear to have roles in sorting NG2 to subcellular microdomains destined to become retraction fibers. Neither of these structural features alone is sufficient to allow optimal targeting of NG2 to retraction fibers, but together they promote efficient localization of the proteoglycan to these sites. This pattern of NG2 sorting seems to be necessary for optimal retraction fiber formation, as cells expressing poorly targeted NG2 mutants are noticeably deficient in their ability to extend retraction fibers. Furthermore, retraction fiber formation correlates strongly with the tendency of cells to assume a polarized morphology with NG2-positive retraction fibers at one pole of the cell and actin-rich lamellipodia at the other. This polarization can be triggered either through engagement of NG2 by the substratum or by exposure to lysophosphatidic acid, a potent activator of the rho GTPase. These results suggest a possible role for NG2 in regulating rho-dependent mechanisms in the trailing processes of motile cells.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Stallcup WB,Dahlin-Huppe Kkeywords:
subject
Has Abstractpub_date
2001-06-01 00:00:00pages
2315-25issue
Pt 12eissn
0021-9533issn
1477-9137journal_volume
114pub_type
杂志文章abstract：:The signal recognition particle (SRP) is a ribonucleoprotein machine that controls the translation and intracellular sorting of membrane and secreted proteins. The SRP contains a core RNA subunit with which six proteins are assembled. Recent work in both yeast and mammalian cells has identified the nucleolus as a poss...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01726
更新日期：2005-03-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01061
更新日期：2004-04-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-04-01 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2007-06-15 00:00:00
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journal_title：Journal of cell science
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doi：
更新日期：2002-08-01 00:00:00
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更新日期：2008-05-15 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2013-02-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2013-02-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2014-04-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-02-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2017-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-12-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
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更新日期：2007-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
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doi：
更新日期：2000-09-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-08-01 00:00:00
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journal_title：Journal of cell science
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journal_title：Journal of cell science
pub_type： 杂志文章
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journal_title：Journal of cell science
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更新日期：2006-07-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2009-04-15 00:00:00
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pub_type： 杂志文章
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更新日期：2009-02-01 00:00:00
