Abstract：
:Endocytosis of caveolae has previously been implicated in the repair of plasma membrane wounds. Here, we show that caveolin-1-deficient fibroblasts lacking caveolae upregulate a tubular endocytic pathway and have a reduced capacity to reseal after permeabilization with pore-forming toxins compared with wild-type cells. Silencing endophilin-A2 expression inhibited fission of endocytic tubules and further reduced plasma membrane repair in cells lacking caveolin-1, supporting a role for tubular endocytosis as an alternative pathway for the removal of membrane lesions. Endophilin-A2 was visualized in association with cholera toxin B-containing endosomes and was recruited to recently formed intracellular vacuoles containing Trypanosoma cruzi, a parasite that utilizes the plasma membrane wounding repair pathway to invade host cells. Endophilin-A2 deficiency inhibited T. cruzi invasion, and fibroblasts deficient in both caveolin-1 and endophilin-A2 did not survive prolonged exposure to the parasites. These findings reveal a novel crosstalk between caveolin-1 and endophilin-A2 in the regulation of clathrin-independent endocytosis and plasma membrane repair, a process that is subverted by T. cruzi parasites for cell invasion.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Corrotte M,Cerasoli M,Maeda FY,Andrews NWdoi
10.1242/jcs.249524subject
Has Abstractpub_date
2020-12-01 00:00:00issue
5eissn
0021-9533issn
1477-9137pii
jcs.249524journal_volume
134pub_type
杂志文章abstract：:Two of the best understood somatic cell mRNA cytoplasmic trafficking elements are those governing localization of beta-actin and myelin basic protein mRNAs. These cis-acting elements bind the trans-acting factors fibroblast ZBP-1 and hnRNP A2, respectively. It is not known whether these elements fulfil other roles in ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00137
更新日期：2002-12-01 00:00:00
abstract：:SNARE [soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) receptor] proteins control the membrane-fusion events of eukaryotic membrane-trafficking pathways. Specific vesicular and target SNAREs operate in specific trafficking routes, but the degree of specificity of SNARE functions is still elus...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.007948
更新日期：2007-09-15 00:00:00
abstract：:This study describes the functional characterization of two cell lines derived from the proximal convoluted (PKSV-PCT cells) and proximal straight (PKSV-PR) tubules microdissected out from kidneys of transgenic mice harboring the simian virus 40 (SV40) large T and small t antigens placed under the control of the rat L...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1993-03-01 00:00:00
abstract：:Centaurin-alpha(1) is a phosphatidylinositol 3,4,5-trisphosphate binding protein as well as a GTPase activating protein (GAP) for the ADP-ribosylation factor (ARF) family of small GTPases. To further understand its cellular function, we screened a rat brain cDNA library using centaurin-alpha(1) as bait to identify cen...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02369
更新日期：2005-06-01 00:00:00
abstract：:The role of basal epithelial cells in prostatic function, development and carcinogenesis is unknown. The ability of basal prostatic epithelial cells to acquire a luminal phenotype was explored in vitro using the NRP-152 rat dorsal-lateral prostate epithelial cell line as a model system. NRP-152, which was spontaneousl...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:Actomyosin stress fibers (SFs) enable cells to exert traction on planar extracellular matrices (ECMs) by tensing focal adhesions (FAs) at the cell-ECM interface. Although it is widely appreciated that the spatial and temporal distribution of these tensile forces play key roles in polarity, motility, fate choice, and o...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.119032
更新日期：2013-07-15 00:00:00
abstract：:Mutations in inositol polyphosphate 5-phosphatase E (INPP5E) cause the ciliopathies known as Joubert and MORM syndromes; however, the role of INPP5E in ciliary biology is not well understood. Here, we describe an interaction between INPP5E and AURKA, a centrosomal kinase that regulates mitosis and ciliary disassembly,...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.161323
更新日期：2015-01-15 00:00:00
abstract：:Despite observations that the lacrimal gland has been identified as the principal source of dimeric immunoglobulin A (dIgA) in tears, the mechanism used by lacrimal gland acinar cells (LGACs) to transcytose dIgA produced by interstitial plasma cells is not well-characterized. This study identifies a transcytotic pathw...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.088906
更新日期：2011-10-15 00:00:00
abstract：:The 5T4 oncofoetal antigen is a 72 kDa glycoprotein defined by a monoclonal antibody raised against human placental trophoblast and is expressed in many different carcinomas but detected only at low levels in some normal epithelia. Immunohistochemical analysis of the patterns of expression in colorectal carcinomas has...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-08-01 00:00:00
abstract：:The influence of extracellular environmental factors on preimplantation rat blastocysts was tested by determining the number of embryos which escaped from their zonae pellucidae, followed by attachment and outgrowth of trophoblast giant cells, after 72 h in culture Uterine luminal ocmponents from individual females, o...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1977-06-01 00:00:00
abstract：:Postsynaptic neuronal dendrites undergo functional and morphological changes in response to pathologically excessive synaptic activation. Although rapid formation of segmental focal swelling (varicosity) is the most prominent hallmark in such excitotoxic injury, little is known about the pathophysiological function of...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
abstract：:In adipocytes and muscle cells, the GLUT4 glucose transporter isoform is present in intracellular vesicles which continuously recycle between an intracytoplasmic location and the plasma membrane. It is not clear whether the GLUT4-vesicles represent a specific kind of vesicle or resemble typical secretory granules or s...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-06-01 00:00:00
abstract：:The intraerythrocytic developmental cycle of Plasmodium falciparum is completed with the release of up to 32 invasive daughter cells, the merozoites, into the blood stream. Before release, the final step of merozoite development is the assembly of the cortical pellicle, a multi-layered membrane structure. This unique ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.181230
更新日期：2016-02-15 00:00:00
abstract：:Xenopus embryos initiate replication at random closely spaced sites until a certain concentration of nuclei is achieved within the embryo, after which fewer, more specific chromosomal sites are utilized as origins. We have examined the relationship between nucleo-cytosolic ratio and origin specification when Chinese h...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-10-01 00:00:00
abstract：:Ena/VASP proteins are actin-binding proteins that localize to actin stress fibres, the tips of filopodia and the lamellipodial leading edge. In the past few years, a number of seemingly conflicting studies have confused the Ena/VASP field, pointing to roles for these proteins in both promotion and inhibition of actin-...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.00218
更新日期：2002-12-15 00:00:00
abstract：:In neutrophils and other phagocytic cells, the small GTPase Rac is an essential regulator of a multi-component NADPH oxidase that produces high levels of superoxide, which kills invading pathogens. In many other cell types, Rac and newly discovered relatives of the neutrophil burst oxidase and its subunits have been f...
journal_title：Journal of cell science
pub_type： 杂志文章,评审
doi：10.1242/jcs.00937
更新日期：2004-01-15 00:00:00
abstract：:The alpha6beta4 integrin is located at the basal surface of keratinocytes, in hemidesmosomal structures that mediate stable adhesion of epidermal cells to the underlying basement membrane component laminin-5. The absence of alpha6beta4 integrin causes junctional epidermolysis bullosa, a severe blistering disease of th...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01689
更新日期：2005-03-01 00:00:00
abstract：:Oocytes of Xenopus laevis in pachytene and early diplotene of meiosis have been studied using the Miller spreading technique. Transcription first appears in germinal vesicles 25-40 micrometers in diameter, when the oocyte is in early diplotene. Transcription at this stage consists of arrays of short RNP transcripts, i...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1980-08-01 00:00:00
abstract：:Vertebrate oocytes proceed through the first and the second meiotic division without an intervening S-phase to become haploid. Although DNA replication does not take place, unfertilized oocytes acquire the competence to replicate DNA one hour after the first meiotic division by accumulating an essential factor of the ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.166553
更新日期：2015-07-15 00:00:00
abstract：:To understand the mechanism involved in the apical sorting of glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) we fused to the C-terminus of GFP the GPI-anchor-attachment signal of the folate receptor (FR) or of the prion protein (PrP), two native GPI-anchored proteins that are sorted apically or basolat...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.036038
更新日期：2008-12-15 00:00:00
abstract：:The myosin regulatory light chain (RLC) of Dictyostelium discoideum is phosphorylated at a single serine site in response to chemoattractant. To investigate the role of the phosphorylation of RLC in both motility and chemotaxis, mutants were generated in which the single phosphorylatable serine was replaced with a non...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-04-15 00:00:00
abstract：:Prox1 plays pivotal roles during embryonic lymphatic development and maintenance of adult lymphatic systems by modulating the expression of various lymphatic endothelial cell (LEC) markers, such as vascular endothelial growth factor receptor 3 (VEGFR3). However, the molecular mechanisms by which Prox1 transactivates i...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.083998
更新日期：2011-08-15 00:00:00
abstract：:Neutrophil leucocytes circulate in plasma, which contains broad-spectrum antiproteases such as alpha-2-macroglobulin and alpha-1-antitrypsin, and move into environments in which antiproteases are present. Plasma antiproteases significantly reduce the adhesiveness of leucocytes in both static and dynamic adhesion assay...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：:Many of the cilia- and flagella-specific integral membrane proteins identified to date function to sense the extracellular milieu, and there is considerable interest in defining pathways for targeting such proteins to these sensory organelles. The flagellar glucose transporter of Leishmania mexicana, LmxGT1, is target...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.103028
更新日期：2012-07-15 00:00:00
abstract：:A number of structural and functional subnuclear compartments have been described, including regions exclusive of chromosomes previously hypothesized to form a reactive nuclear space. We have now explored this accessible nuclear space and interchromosomal nucleoplasmic domains experimentally using Xenopus vimentin eng...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
abstract：:Misfolded endoplasmic reticulum (ER) proteins are dislocated towards the cytosol and degraded by the ubiquitin-proteasome system in a process called ER-associated protein degradation (ERAD). During infection with human cytomegalovirus (HCMV), the viral US2 protein targets HLA class I molecules (HLA-I) for degradation ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.206839
更新日期：2017-09-01 00:00:00
abstract：:The dynamics of cell adhesion sites control cell morphology and motility. Adhesion-site turnover is thought to depend on the local availability of the acidic phospholipid phosphatidylinositol-4,5-bisphosphate (PIP(2)). PIP(2) can bind to many cell adhesion proteins such as vinculin and talin, but the consequences of t...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.01734
更新日期：2005-04-01 00:00:00
abstract：:The alternative lengthening of telomeres (ALT) mechanism allows cancer cells to escape senescence and apoptosis in the absence of active telomerase. A characteristic feature of this pathway is the assembly of ALT-associated promyelocytic leukemia (PML) nuclear bodies (APBs) at telomeres. Here, we dissected the role of...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.148296
更新日期：2015-05-15 00:00:00
abstract：:A method is reported for preparing surface (plasma) membranes from adrenal tumour (Y-1) cells. The procedure is based upon homogenization in hypotonic ZnCl2 followed by sedimentation through two sucrose density gradients. The purified membranes consist of large sheets of membrane. The identity and purity of the membra...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1985-08-01 00:00:00
abstract：:Dishevelled-3 (Dvl3) is a multivalent scaffold protein that is essential to Wnt signaling during development. Although Dvl-based punctae have been visualized by fluorescence microscopy; the physical nature and dynamic character of the such complexes are enigmatic. We use steric-exclusion chromatography, affinity pull-...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.075275
更新日期：2010-11-01 00:00:00
