Abstract:
:The contractile vacuole complex (CVC) of Trypanosoma cruzi, the etiologic agent of Chagas disease, collects and expels excess water as a mechanism of regulatory volume decrease after hyposmotic stress; it also has a role in cell shrinking after hyperosmotic stress. Here, we report that, in addition to its role in osmoregulation, the CVC of T. cruzi has a role in the biogenesis of acidocalcisomes. Expression of dominant-negative mutants of the CVC-located small GTPase Rab32 (TcCLB.506289.80) results in lower numbers of less-electron-dense acidocalcisomes, lower content of polyphosphate, lower capacity for acidocalcisome acidification and Ca(2+) uptake that is driven by the vacuolar proton pyrophosphatase and the Ca(2+)-ATPase, respectively, as well as less-infective parasites, revealing the role of this organelle in parasite infectivity. By using fluorescence, electron microscopy and electron tomography analyses, we provide further evidence of the active contact of acidocalcisomes with the CVC, indicating an active exchange of proteins between the two organelles.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Niyogi S,Jimenez V,Girard-Dias W,de Souza W,Miranda K,Docampo Rdoi
10.1242/jcs.169466subject
Has Abstractpub_date
2015-06-15 00:00:00pages
2363-73issue
12eissn
0021-9533issn
1477-9137pii
jcs.169466journal_volume
128pub_type
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