Characterization of mucins and proteoglycans synthesized by a mucin-secreting HT-29 cell subpopulation.

abstract：:In hepatocytes, PLIN2 is the major protein coating lipid droplets (LDs), an organelle the hepatitis C virus (HCV) hijacks for virion morphogenesis. We investigated the consequences of PLIN2 deficiency on LDs and on HCV infection. Knockdown of PLIN2 did not affect LD homeostasis, likely due to compensation by PLIN3, bu...

journal_title：Journal of cell science

authors： Lassen S,Grüttner C,Nguyen-Dinh V,Herker E

更新日期：2019-01-09 00:00:00

abstract：:Ligand-dependent transcriptional regulation by nuclear receptors is believed to be mediated by intermediary factors (TIFs) acting on remodelling of the chromatin structure and/or the activity of the transcriptional machinery. The putative transcriptional mediator TIF1alpha is a nuclear protein kinase that has been ide...

journal_title：Journal of cell science

authors： Remboutsika E,Lutz Y,Gansmuller A,Vonesch JL,Losson R,Chambon P

更新日期：1999-06-01 00:00:00

abstract：:Glomerular mesangial cells (MCs) are central to the pathogenesis of progressive glomeruli-associated renal diseases. However, molecular mechanisms underlying changes in MC functions still remain poorly understood. Here, we show that in MCs, the urokinase-type plasminogen activator (uPA) induces, via its specific recep...

journal_title：Journal of cell science

authors： Shushakova N,Tkachuk N,Dangers M,Tkachuk S,Park JK,Hashimoto K,Haller H,Dumler I

更新日期：2005-06-15 00:00:00

abstract：:Schizosaccharomyces pombe cells divide by medial fission throughout contraction of an actomyosin ring and deposition of a multilayered division septum that must be cleaved to release the two daughter cells. Although many studies have focused on the actomoysin ring and septum assembly, little information is available c...

journal_title：Journal of cell science

authors： Martín-Cuadrado AB,Dueñas E,Sipiczki M,Vázquez de Aldana CR,del Rey F

更新日期：2003-05-01 00:00:00

abstract：:Integrin and receptor tyrosine kinase signalling networks cooperate to regulate various biological functions. The molecular details underlying the integration of both signalling networks remain largely uncharacterized. Here we identify a signalling module composed of a fibronectin-α5β1-integrin-integrin-linked-kinase ...

journal_title：Journal of cell science

authors： Azimifar SB,Böttcher RT,Zanivan S,Grashoff C,Krüger M,Legate KR,Mann M,Fässler R

更新日期：2012-01-15 00:00:00

abstract：:The fate of concanavalin A (Con A) bound to normal and enucleated L cells was followed at the ultrastructural level over a 20-h period. In both enucleates and normal cells the Con A is seen to be distributed in a uniform manner over the entire cell surface following a 30-min pulse with a low concentration of Con A. In...

journal_title：Journal of cell science

authors： Wise GE

更新日期：1976-12-01 00:00:00

abstract：:Sox7, Sox17 and Sox18 constitute group F of the Sox family of HMG box transcription factor genes. Dominant-negative mutations in Sox18 underlie the cardiovascular defects observed in ragged mutant mice. By contrast, Sox18(-/-) mice are viable and fertile, and display no appreciable anomaly in their vasculature, sugges...

journal_title：Journal of cell science

authors： Matsui T,Kanai-Azuma M,Hara K,Matoba S,Hiramatsu R,Kawakami H,Kurohmaru M,Koopman P,Kanai Y

更新日期：2006-09-01 00:00:00

abstract：:Epithelia are compact tissues comprising juxtaposed cells that function as mechanical and chemical barriers between the body and the environment. This barrier relies, in part, on adhesive contacts within adherens junctions, which are formed and stabilized by E-cadherin and catenin proteins linked to the actomyosin cyt...

journal_title：Journal of cell science

authors： Le Bras S,Le Borgne R

更新日期：2014-12-15 00:00:00

abstract：:Errors during the first meiotic division are common in our species, but virtually all occur during female meiosis. The reason why oogenesis is more error prone than spermatogenesis remains unknown. Normal segregation of homologous chromosomes at the first meiotic division (MI) requires coordinated behavior of the sist...

journal_title：Journal of cell science

authors： Hodges CA,LeMaire-Adkins R,Hunt PA

更新日期：2001-07-01 00:00:00

abstract：:SH4 domains provide bipartite membrane-targeting signals for oncogenic Src family kinases. Here we report the induction of non-apoptotic plasma membrane (PM) blebbing as a novel and conserved activity of SH4 domains derived from the prototypic Src kinases Src, Fyn, Yes and Lck as well as the HASPB protein of Leishmani...

journal_title：Journal of cell science

authors： Tournaviti S,Hannemann S,Terjung S,Kitzing TM,Stegmayer C,Ritzerfeld J,Walther P,Grosse R,Nickel W,Fackler OT

更新日期：2007-11-01 00:00:00

abstract：:Glycosylphosphatidylinositol-anchored proteins on the egg surface have been proposed to play a role in gamete fusion on the basis of in vitro experiments. We tested this hypothesis by asking if oocyte GPI-anchored proteins are required for fertilization in vivo. Oocyte-specific knockout mice were created using the Cre...

journal_title：Journal of cell science

authors： Alfieri JA,Martin AD,Takeda J,Kondoh G,Myles DG,Primakoff P

更新日期：2003-06-01 00:00:00

abstract：:The microtubule-associated protein tau interacts with the SH3 domain of non-receptor Src family protein tyrosine kinases. A potential consequence of the SH3 interaction is the upregulation of tyrosine kinase activity. Here we investigated the activation of Src or Fyn by tau, both in vitro and in vivo. Tau increased th...

journal_title：Journal of cell science

authors： Sharma VM,Litersky JM,Bhaskar K,Lee G

更新日期：2007-03-01 00:00:00

abstract：:Prox1 is a master regulator for the development of lymphatic vasculature and the induction of lymphangiogenesis. In this study, we identified Prox1 as a new target for small ubiquitin-like modifier 1 (SUMO-1). Lysine 556 (K556) was found to be the major sumoylation site for Prox1 in vitro and in vivo. Mutation of this...

journal_title：Journal of cell science

authors： Pan MR,Chang TM,Chang HC,Su JL,Wang HW,Hung WC

更新日期：2009-09-15 00:00:00

abstract：:An analysis of the concanavalinA binding polypeptide components of bovine tongue epithelial desmosomes reveals that in addition to the known desmosomal glycoproteins of 100/115K (the 'desmocollins'), 140K and 160/165K ('desmoglein 1') there is an uncharacterized glycoprotein of 125K (K = Mr x 10(-3). This latter polyp...

journal_title：Journal of cell science

authors： Jones JC

更新日期：1988-02-01 00:00:00

abstract：:Sister chromatid cohesion is essential for proper segregation of the genome in mitosis and meiosis. Central to this process is cohesin, a multi-protein complex conserved from yeast to human. Previous genetic studies in fungi have identified Pds5/BimD/Spo76 as an additional factor implicated in cohesion. Here we descri...

journal_title：Journal of cell science

authors： Losada A,Yokochi T,Hirano T

更新日期：2005-05-15 00:00:00

abstract：:New crystal structures of the kinesin motors differ from previously described motor-ADP atomic models, showing striking changes both in the switch I region near the nucleotide-binding cleft and in the switch II or 'relay' helix at the filament-binding face of the motor. The switch I region, present as a short helix fl...

journal_title：Journal of cell science

authors： Kull FJ,Endow SA

更新日期：2002-01-01 00:00:00

abstract：:During meiosis, cohesion between sister chromatids is required for normal levels of homologous recombination, maintenance of chiasmata and accurate chromosome segregation during both divisions. In Drosophila, null mutations in the ord gene abolish meiotic cohesion, although how ORD protein promotes cohesion has remain...

journal_title：Journal of cell science

authors： Khetani RS,Bickel SE

更新日期：2007-09-01 00:00:00

abstract：:The aryl hydrocarbon receptor (AhR) is a transcriptional regulator of genes involved in xenobiotic metabolism. Increasingly clear is also the role of the AhR in the control of cell growth and proliferation. By analyzing differential patterns of gene expression between wild-type (AhR+/+) and null (AhR-/-) mouse embryo ...

journal_title：Journal of cell science

authors： Santiago-Josefat B,Mulero-Navarro S,Dallas SL,Fernandez-Salguero PM

更新日期：2004-02-29 00:00:00

abstract：:MicroRNAs are short non-coding RNAs involved in post-transcriptional regulation of multiple messenger RNA targets. The miR-1/miR-206 family is expressed during skeletal muscle differentiation and is an integral component of myogenesis. To better understand miR-1/miR-206 function during myoblast differentiation we iden...

journal_title：Journal of cell science

authors： Goljanek-Whysall K,Pais H,Rathjen T,Sweetman D,Dalmay T,Münsterberg A

更新日期：2012-08-01 00:00:00

abstract：:Endocytosis and recycling of membrane proteins are key processes for nutrient uptake, receptor signaling and synaptic transmission. Different steps in these fission and fusion cycles have been proposed to be regulated by physiological changes in plasma membrane (PM) phosphatidylinositol (4,5)-bisphosphate [PtdIns(4,5)...

journal_title：Journal of cell science

authors： Abe N,Inoue T,Galvez T,Klein L,Meyer T

更新日期：2008-05-01 00:00:00

abstract：:Basonuclin is a zinc finger protein mainly expressed in keratinocytes of the basal layer of epidermis and the outer root sheath of hair follicles. It is also found in abundance in the germ cells of testis and ovary. In cultured keratinocytes, basonuclin is associated with chromatin in all phases of the cell cycle, inc...

journal_title：Journal of cell science

authors： Tseng H,Biegel JA,Brown RS

更新日期：1999-09-01 00:00:00

abstract：:IGFBP3 is a multi-functional protein that has IGF-dependent and IGF-independent actions in cultured cells. Here we show that the IGF binding domain (IBD), nuclear localization signal (NLS) and transactivation domain (TA) are conserved and functional in zebrafish Igfbp3. The in vivo roles of these domains were investig...

journal_title：Journal of cell science

authors： Zhong Y,Lu L,Zhou J,Li Y,Liu Y,Clemmons DR,Duan C

更新日期：2011-06-01 00:00:00

abstract：:In the fission yeast Schizosaccharomyces pombe, a series of diploid mutants divides at smaller cell sizes than wild type. In these smaller strains, the mean gene concentration (defined by previous authors as the DNA to protein ratio) is higher than in wild type. Such an increase in gene concentration should also incre...

journal_title：Journal of cell science

authors： Fraser RS,Nurse P

更新日期：1979-02-01 00:00:00

abstract：:Centralspindlin, a complex of the kinesin-6-family member MKLP1 and MgcRacGAP (also known as Kif23 and Racgap1, respectively), is required for cytokinesis and cell-cell junctions. During anaphase, Centralspindlin accumulates at overlapping central spindle microtubules and directs contractile ring formation by recruiti...

journal_title：Journal of cell science

authors： Breznau EB,Murt M,Blasius TL,Verhey KJ,Miller AL

更新日期：2017-05-15 00:00:00

abstract：:Na+/H+ exchangers (NHEs) are transporters that exchange sodium and proton ions across the plasma membrane at the expense of their chemical gradient. In higher eukaryotes these transporters exist as multiple specialized isoforms. For example, NHE1, the ubiquitously expressed form is a major pH-regulating system whereas...

journal_title：Journal of cell science

authors： Noel J,Roux D,Pouysségur J

更新日期：1996-05-01 00:00:00

abstract：:In the cytoplasm of chlorenchyma cells in primary Origanum dictamnus leaves, rectangular prism-like crystals occur, which, as shown by pepsin digestion, are proteinaceous. In sections they usually show either a square lattice or striations running parallel to the short or long axis. In both cases the interspacings are...

journal_title：Journal of cell science

authors： Bosabalidis AM,Papadopoulos D

更新日期：1983-11-01 00:00:00

abstract：:Semaphorin 3A (Sema3A), a secretory semaphorin, exerts various biological actions through a complex between neuropilin-1 and plexin-As (PlexAs). Sema3A induces retrograde signaling, which is involved in regulating dendritic localization of GluA2 (also known as GRIA2), an AMPA receptor subunit. Here, we investigated a ...

journal_title：Journal of cell science

authors： Yamashita N,Yamane M,Suto F,Goshima Y

更新日期：2016-05-01 00:00:00

abstract：:Here we report Drosophila Waharan (Wah), a 170-kD predominantly nuclear protein with two potential human homologues, as a newly identified regulator of endosomal trafficking. Wah is required for neuromuscular-junction development and muscle integrity. In muscles, knockdown of Wah caused novel accumulations of tightly ...

journal_title：Journal of cell science

authors： Lone M,Kungl T,Koper A,Bottenberg W,Kammerer R,Klein M,Sweeney ST,Auburn RP,O'Kane CJ,Prokop A

更新日期：2010-07-15 00:00:00

abstract：:The post-translational modifications of the G protein of vesicular stomatitis virus, described in the preceding paper, indicate that its transport is arrested by carbonylcyanide m-chlorophenylhydrazone (CCCP) in or near the trans-Golgi. Immunofluorescence microscopy of BHK-21 cells infected with vesicular stomatitis v...

journal_title：Journal of cell science

authors： Burkhardt JK,Hester S,Argon Y

更新日期：1989-04-01 00:00:00

abstract：:By an improved freeze-fracture device, a number of double replicas were obtained from the rod disk membranes of the carp retina for investigation of complementary, continuing, and transitional relationships between fracture faces. Some separate particles found along the smooth edge bordering on the adjacent interdisk ...

journal_title：Journal of cell science

authors： Tonosaki A,Washioka H,Yamasaki M,Mizoguchi J

更新日期：1980-04-01 00:00:00