Abstract：
:Protein traffic moving from the endoplasmic reticulum (ER) to the Golgi complex in mammalian cells passes through the tubulovesicular membrane clusters of the ER-Golgi intermediate compartment (ERGIC), the marker of which is the lectin ERGIC-53. The dynamic nature and functional role of the ERGIC have been debated for quite some time. In the most popular current view, the ERGIC clusters are mobile transport complexes that deliver secretory cargo from ER-exit sites to the Golgi. Recent live-cell imaging data revealing the formation of anterograde carriers from stationary ERGIC-53-positive membranes, however, suggest a stable compartment model in which ER-derived cargo is first shuttled from ER-exit sites to stationary ERGIC clusters in a COPII-dependent step and subsequently to the Golgi in a second vesicular transport step. This model can better accommodate previous morphological and functional data on ER-to-Golgi traffic. Such a stationary ERGIC would be a major site of anterograde and retrograde sorting that is controlled by coat proteins, Rab and Arf GTPases, as well as tethering complexes, SNAREs and cytoskeletal networks. The ERGIC also contributes to the concentration, folding, and quality control of newly synthesized proteins.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Appenzeller-Herzog C,Hauri HPdoi
10.1242/jcs.03019subject
Has Abstractpub_date
2006-06-01 00:00:00pages
2173-83issue
Pt 11eissn
0021-9533issn
1477-9137pii
119/11/2173journal_volume
119pub_type
杂志文章,评审abstract：:A monoclonal antibody, specific to phosphoproteins in mitotic HeLa cells was found to crossreact with a similar set of proteins in embryos of the nematode, Caenorhabditis elegans. In C. elegans, as in mammalian cells, the highly conserved antigenic epitope is associated with a family of high molecular weight polypepti...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1987-03-01 00:00:00
abstract：:Earlier studies have established that the average speed of a replication fork is two to three times slower in early S-phase than in late S-phase and that the intracellular 2'-deoxyribonucleoside 5'-triphosphate pools grow during S-phase. In this study, the effect of the exogenous 2'-deoxyribonucleoside 5'-triphosphate...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-02-01 00:00:00
abstract：:The novel NSP gene was previously shown to encode, among a variety of neuroendocrine cell types, two 3'-overlapping transcripts, a 3.4 kb one for NSP-A (776 amino acids) and a 1.8 kb one for NSP-C (208 amino acids). The deduced proteins, which were predicted to possess distinct amino-terminal regions, appeared to exhi...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
abstract：:Most tissues harbor a substantial population of resident macrophages. Here, we elucidate a functional link between the Slc7a7 cationic amino acid transporter and tissue macrophages. We identified a mutant zebrafish devoid of microglia due to a mutation in the slc7a7 gene. We found that in Slc7a7-deficient larvae, macr...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.249037
更新日期：2020-10-22 00:00:00
abstract：:We have used MPM-2, a monoclonal antibody raised against mitotic HeLa cells, to stain a Drosophila cell line, whole mounts of Drosophila embryos, and sectioned tissue from embryonic and larval stages of development. MPM-2 recognizes a major phosphoprotein of approximately 125 X 10(3) Mr in Drosophila tissue culture ce...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1987-02-01 00:00:00
abstract：:The centrosome and cilium are organelles with important roles in microtubule organisation, cell division, cell signalling, embryogenesis and tissue homeostasis. The two organelles are mutually exclusive. The centriole/basal body is found at the core of the centrosome (centriole) or at the base of the cilium (basal bod...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.196642
更新日期：2017-10-01 00:00:00
abstract：:We have generated novel lentiviral vectors to integrate various connexin cDNAs into primary, non-dividing cells. We have used these vectors to test whether proper control of insulin secretion depends on a specific connexin isoform and/or on its level of expression. We have observed that transduced connexin32, connexin...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00442
更新日期：2003-06-01 00:00:00
abstract：:Polypeptide chains synthesized by membrane-bound ribosomes are translocated through, and integrated into, the endoplasmic reticulum (ER) membrane by means of the protein translocation channel, the translocon. Positive charges on the nascent chain determine the orientation of the hydrophobic segment as it is inserted i...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.086850
更新日期：2011-12-15 00:00:00
abstract：:Rac1 is a small Rho family GTPase that regulates changes in cell morphology associated with cell spreading and migration. Integrin-mediated adhesion is known to activate Rac1 and to regulate the interaction of Rac1 with downstream effectors. Currently, it is not clear how integrins signal Rac1 activation following cel...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.00109
更新日期：2002-11-15 00:00:00
abstract：:Cilia and flagella are conserved eukaryotic organelles important for motility and sensory. The RanGTPase, best known for nucleocytoplasmic transport functions, may also play a role in protein trafficking into the specialized flagellar/ciliary compartments, although the regulatory mechanisms controlling Ran activity at...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.148015
更新日期：2014-11-15 00:00:00
abstract：:Errors during the first meiotic division are common in our species, but virtually all occur during female meiosis. The reason why oogenesis is more error prone than spermatogenesis remains unknown. Normal segregation of homologous chromosomes at the first meiotic division (MI) requires coordinated behavior of the sist...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
abstract：:The vacuolar H(+)-ATPase (V-ATPase) is a multi-subunit enzyme that plays important roles in eukaryotic cells. In Dictyostelium, it is found primarily in membranes of the contractile vacuole complex, where it energizes fluid accumulation by this osmoregulatory organelle and also in membranes of endolysosomes, where it ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-07-15 00:00:00
abstract：:The primary structures of three human forms of secretory carrier membrane proteins (SCAMPs) have been deduced from full-length clones isolated from a HeLa cell cDNA library and confirmed by a combination of comparison to expressed sequence tags, microsequencing of purified protein, and in vitro transcription and trans...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：:Many adherent cells in vitro are surrounded by a transparent exclusion zone or halo, several micrometers thick, which red blood cells, bacteria and carbon particles cannot penetrate. This halo is rapidly and specifically removed by hyaluronidase and its high degree of hydration is demonstrated by the fact that, althou...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1983-07-01 00:00:00
abstract：:Pieces of small intestine from newborn, I- and 6-day-old piglets were incubated in vitro and ligated segments were incubated in vivo with polycationic ferritin (PCF) to determine the distribution and possible role in protein uptake of anionic sites on membranes at the luminal surfaces of the epithelial cells. Most PCF...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1980-12-01 00:00:00
abstract：:Phagocytosis is an actin-based process used by macrophages to clear particles greater than 0.5 microm in diameter. In addition to its role in immunological responses, phagocytosis is also necessary for tissue remodeling and repair. To prevent catastrophic autoimmune reactions, phagocytosis must be tightly regulated. I...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2002-02-15 00:00:00
abstract：:Brain-derived neurotrophic factor (BDNF) plays an important role in neuronal survival, neurite outgrowth and synaptic plasticity by activating the receptor tropomyosin receptor kinase B (TrkB, also known as NTRK2). TrkB has been shown to undergo recycling after BDNF stimulation. We have previously reported that full-l...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.160259
更新日期：2015-03-15 00:00:00
abstract：:In sciarid flies (Diptera, Sciaridae), one or two paternally derived X chromosomes are discarded from the soma at early cleavages to determine the sex of the embryo (XX, females; X0, males). X chromosome(s) elimination is achieved by an abnormal anaphase segregation so that X sister chromatids do not reach the poles a...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.128900
更新日期：2013-07-15 00:00:00
abstract：:Increased deposition of collagen in extracellular matrix (ECM) leads to increased tissue stiffness and occurs in breast tumors. When present, this increases tumor invasion and metastasis. Precisely how this deposition is regulated and maintained in tumors is unclear. Much has been learnt about mechanical signal transd...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.180539
更新日期：2016-05-15 00:00:00
abstract：:MicroRNAs (miRNAs) play important roles in the development of skeletal muscle. In our previous study, expression of miR-195 and miR-497 were shown to be upregulated during muscle development in pigs. In this study, we investigated the roles of these two miRNAs in myogenesis and analyzed their transcriptional regulatio...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.174235
更新日期：2016-01-01 00:00:00
abstract：:Neural stem cells (NSCs) typically show efficient self-renewal and selective differentiation. Their invasion potential, however, is not well studied. In this study, Sox2-positive NSCs from the E14.5 rat cortex were found to be non-invasive and showed only limited migration in vitro. By contrast, FGF2-expanded NSCs sho...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.125757
更新日期：2013-08-15 00:00:00
abstract：:If mitotic-arrested, cold-stored HeLa cells are incubated at 37 degrees C a proportion of the population divides by an aberrant process which we have called subdivision by extrusion. This process has been studied by time-lapse photography and shown to differ from normal cleavage in several respects. The cell surface b...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1976-11-01 00:00:00
abstract：:Apoptosis, or programmed cell death, involves a cascade of regulatory events leading to the activation of specific proteases. However, the key substrates for these proteases remain to be identified. We previously demonstrated that levels of five unidentified polypeptides were specifically increased in neurons from emb...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:Tumor necrosis factor alpha-converting enzyme (TACE/ADAM17) has been implicated in the inducible shedding of numerous cell surface proteins. In light of this, the regulation of TACE catalytic activation and protein maturation has recently received considerable interest in the context of extracellular signal-regulated ...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.02357
更新日期：2005-06-01 00:00:00
abstract：:B chromosomes are centric chromosomal fragments present in thousands of eukaryotic genomes. Because most B chromosomes are non-essential, they can be lost without consequence. In order to persist, however, some B chromosomes can impose strong forms of intra-genomic conflict. An extreme case is the paternal sex ratio (...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.113423
更新日期：2012-11-01 00:00:00
abstract：:Mammalian, or mechanistic, target of rapamycin complex 2 (mTORC2) regulates a variety of vital cellular processes, and its aberrant functioning is often associated with various diseases. Rictor is a peculiar and distinguishing mTORC2 component playing a pivotal role in controlling its assembly and activity. Among exta...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.236505
更新日期：2019-11-14 00:00:00
abstract：:The distribution of centromeres in the interphase nuclei of human diploid fibroblasts was analyzed using anti-centromere immunofluorescence and laser scanning confocal microscopoy. The positions of the centromeres were placed within the nuclear chromatin distribution and presented some aspects of the dynamics of nucle...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:Although it is well established that embryonic stem (ES) cells have the potential to differentiate into dopamine neurons, the molecular basis of this process, particularly the role of microRNAs (miRNAs), remains largely unknown. Here we report that miR-132 plays a key role in the differentiation of dopamine neurons by...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：10.1242/jcs.086421
更新日期：2012-04-01 00:00:00
abstract：:A high molecular weight tight junction-associated protein, ZO-1, has been demonstrated in liver (hepatocytes) and in both epithelium and endothelium. We carried out studies to examine the presence of the protein in vascular endothelial cell cultures and several other types of cultured cells, and the relationship betwe...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
abstract：:Effective cell-to-cell spreading of the facultative intracellular pathogen Listeria monocytogenes requires the interaction between bacteria and the microfilament system of the host cell. By recruiting actin filaments into a 'comet tail' localized at one pole of the bacterial cell wall, Listeria become mobile and prope...
journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1994-10-01 00:00:00
