SH3YL1 cooperates with ESCRT-I in the sorting and degradation of the EGF receptor.

abstract：:The growth of two human diploid skin fibroblast cell lines, originally grown in medium supplemented with foetal bovine serum and later adapted to medium supplemented with newborn bovine, bovine calf or horse serum, has been studied. Prolonged generation times increased cell volumes and decreased plating efficiencies w...

journal_title：Journal of cell science

authors： Zamansky GB,Arundel C,Nagasawa H,Little JB

更新日期：1983-05-01 00:00:00

abstract：:The fate of concanavalin A (Con A) bound to normal and enucleated L cells was followed at the ultrastructural level over a 20-h period. In both enucleates and normal cells the Con A is seen to be distributed in a uniform manner over the entire cell surface following a 30-min pulse with a low concentration of Con A. In...

journal_title：Journal of cell science

authors： Wise GE

更新日期：1976-12-01 00:00:00

abstract：:Nuclear domains called ND10 or PML nuclear bodies consist of an aggregation of several proteins, most notably PML and Sp100. PML is essential in the nucleation and formation of ND10 as well as in the recruitment of other ND10-associated proteins such as Daxx, pRb, BLM and Sp100. In cells induced to overexpress Sp100, ...

journal_title：Journal of cell science

authors： Negorev D,Ishov AM,Maul GG

更新日期：2001-01-01 00:00:00

abstract：:Excitatory amino acid transporter type 3 (EAAT3, also known as SLC1A1) is a high-affinity, Na(+)-dependent glutamate carrier that localizes primarily within the cell and at the apical plasma membrane. Although previous studies have reported proteins and sequence regions involved in EAAT3 trafficking, the detailed mole...

journal_title：Journal of cell science

authors： Su JF,Wei J,Li PS,Miao HH,Ma YC,Qu YX,Xu J,Qin J,Li BL,Song BL,Xu ZP,Luo J

更新日期：2016-08-15 00:00:00

abstract：:The establishment of a tissue-culture system for epithelial cells derived from human foetal pancreas has recently been reported. Further analyses have now been made on these cells in vitro, together with parallel investigation of the distribution of different cell types within the intact foetal pancreas. Results suppo...

journal_title：Journal of cell science

authors： Harris A,Coleman L

更新日期：1988-05-01 00:00:00

abstract：:Brain-derived neurotrophic factor (BDNF) is encoded by multiple mRNA variants whose differential subcellular distribution constitutes a 'spatial code' for local translation of BDNF and selective morphological remodeling of dendrites. Here, we investigated where BDNF translation takes place and what are the signaling p...

journal_title：Journal of cell science

authors： Baj G,Pinhero V,Vaghi V,Tongiorgi E

更新日期：2016-07-15 00:00:00

abstract：:Axin and conductin (also known as axin2) are structurally related inhibitors of Wnt/β-catenin signalling that promote degradation of β-catenin. Whereas axin is constitutively expressed, conductin is a Wnt target gene implicated in Wnt negative-feedback regulation. Here, we show that axin and conductin differ in their ...

journal_title：Journal of cell science

authors： Bernkopf DB,Hadjihannas MV,Behrens J

更新日期：2015-01-01 00:00:00

abstract：:In sciarid flies (Diptera, Sciaridae), one or two paternally derived X chromosomes are discarded from the soma at early cleavages to determine the sex of the embryo (XX, females; X0, males). X chromosome(s) elimination is achieved by an abnormal anaphase segregation so that X sister chromatids do not reach the poles a...

journal_title：Journal of cell science

authors： Escribá MC,Goday C

更新日期：2013-07-15 00:00:00

abstract：:Neutrophil leucocytes circulate in plasma, which contains broad-spectrum antiproteases such as alpha-2-macroglobulin and alpha-1-antitrypsin, and move into environments in which antiproteases are present. Plasma antiproteases significantly reduce the adhesiveness of leucocytes in both static and dynamic adhesion assay...

journal_title：Journal of cell science

authors： Forrester JV,Lackie JM,Brown AF

更新日期：1983-01-01 00:00:00

abstract：:NEDD8 is an important regulatory factor in many biological processes. However, the substrates for neddylation, and the relationship between the ubiquitin and NEDD8 pathways remain largely unknown. Here, we show that NEDD8 is covalently conjugated to histone 2A (H2A), and that neddylation of H2A antagonizes its ubiquit...

journal_title：Journal of cell science

authors： Li T,Guan J,Huang Z,Hu X,Zheng X

更新日期：2014-05-15 00:00:00

abstract：:We have investigated the effect of nitric oxide (NO) on apoptosis in Swiss 3T3 fibroblasts and compared it to the effect of the nitrosonium cation (NO+). Both species induced apoptosis, confirmed by electron microscopy, propidium iodide staining, DNA laddering and activation of caspases. The kinetics of triggering apo...

journal_title：Journal of cell science

authors： Khan S,Kayahara M,Joashi U,Mazarakis ND,Sarraf C,Edwards AD,Hughes MN,Mehmet H

更新日期：1997-09-01 00:00:00

abstract：:Previously we cloned and characterized the hamster intermediate filament genes coding for vimentin and desmin. It was demonstrated that the cloned desmin gene was expressed after gene transfer and that the newly synthesized protein assembles into intermediate filaments. Here we present data on the transfection of modi...

journal_title：Journal of cell science

authors： van den Heuvel RM,van Eys GJ,Ramaekers FC,Quax WJ,Vree Egberts WT,Schaart G,Cuypers HT,Bloemendal H

更新日期：1987-11-01 00:00:00

abstract：:Gp78 (also known as AMFR), an endoplasmic-reticulum (ER)-associated protein degradation (ERAD) E3 ubiquitin ligase, localizes to mitochondria-associated ER and targets the mitofusin (Mfn1 and Mfn2) mitochondrial fusion proteins for degradation. Gp78 is also the cell surface receptor for autocrine motility factor (AMF)...

journal_title：Journal of cell science

authors： Wang PT,Garcin PO,Fu M,Masoudi M,St-Pierre P,Panté N,Nabi IR

更新日期：2015-08-01 00:00:00

abstract：:Increases in cytosolic Ca(2+) concentration ([Ca(2+)](c)) mediated by inositol (1,4,5)-trisphosphate [Ins(1,4,5)P(3), hereafter InsP(3)] regulate activities that include division, contraction and cell death. InsP(3)-evoked Ca(2+) release often begins at a single site, then regeneratively propagates through the cell as...

journal_title：Journal of cell science

authors： Olson ML,Sandison ME,Chalmers S,McCarron JG

更新日期：2012-11-15 00:00:00

abstract：:end13-1 was isolated in a screen for endocytosis mutants and has been shown to have a post-internalisation defect in endocytic transport as well as a defect in vacuolar protein sorting (Vps(-) phenotype), leading to secretion of newly synthesised vacuolar proteins. Here we demonstrate that END13 is identical to VPS4, ...

journal_title：Journal of cell science

authors： Zahn R,Stevenson BJ,Schröder-Köhne S,Zanolari B,Riezman H,Munn AL

更新日期：2001-05-01 00:00:00

abstract：:T cell activation is accompanied by actin-mediated receptor clustering and reorganization of lipid rafts. It has been suggested that costimulatory molecules might be involved in these processes. We here provide evidence that engagement of the adhesion molecule CD44 initiates cytoskeletal rearrangement and membrane reo...

journal_title：Journal of cell science

authors： Föger N,Marhaba R,Zöller M

更新日期：2001-03-01 00:00:00

abstract：:By an improved freeze-fracture device, a number of double replicas were obtained from the rod disk membranes of the carp retina for investigation of complementary, continuing, and transitional relationships between fracture faces. Some separate particles found along the smooth edge bordering on the adjacent interdisk ...

journal_title：Journal of cell science

authors： Tonosaki A,Washioka H,Yamasaki M,Mizoguchi J

更新日期：1980-04-01 00:00:00

abstract：:The neural cell adhesion molecule NCAM plays an important role during neural development and in the adult brain. To study the intracellular trafficking of NCAM in neurons, two major isoforms, NCAM140 or NCAM180, were expressed in primary cortical neurons and in the rat B35 neuroblastoma cell line. NCAM was endocytosed...

journal_title：Journal of cell science

authors： Diestel S,Schaefer D,Cremer H,Schmitz B

更新日期：2007-11-15 00:00:00

abstract：:Drosophila abnormal spindle (asp) mutants exhibit a mitotic metaphase checkpoint arrest with abnormal spindle poles, which reflects a requirement for Asp for the integrity of microtubule organising centres (MTOCs). In male meiosis, the absence of a strong spindle integrity checkpoint enables asp mutant cells to procee...

journal_title：Journal of cell science

authors： Riparbelli MG,Callaini G,Glover DM,Avides Mdo C

更新日期：2002-03-01 00:00:00

abstract：:Centrioles organise centrosomes and cilia, and these organelles have an important role in many cell processes. In flies, the centriole protein Ana1 is required for the assembly of functional centrosomes and cilia. It has recently been shown that Cep135 (also known as Bld10) initially recruits Ana1 to newly formed cent...

journal_title：Journal of cell science

authors： Saurya S,Roque H,Novak ZA,Wainman A,Aydogan MG,Volanakis A,Sieber B,Pinto DM,Raff JW

更新日期：2016-07-01 00:00:00

abstract：:In adherent cells, the relevance of a physical mechanotransduction pathway provided by the perinuclear actin cap stress fibers has recently emerged. Here, we investigate the impact of a functional actin cap on the cellular adaptive response to topographical cues and uniaxial cyclic strain. Lmna-deficient fibroblasts a...

journal_title：Journal of cell science

authors： Tamiello C,Halder M,Kamps MA,Baaijens FP,Broers JL,Bouten CV

更新日期：2017-02-15 00:00:00

abstract：:The median cell cycle of synchronized NHIK3025 cells grown in Eagle's MEM with 10% foetal calf serum was 23.6 h, compared to 18 h observed earlier in Puck's E2a medium with 30% serum (20% human and 10% horse). This difference is due to prolongation of both G1 and G2 in the MEM type medium. Testosterone and 4-androsten...

journal_title：Journal of cell science

authors： Ostgaard K,Wibe E,Lindmo T,Eik-Nes KB

更新日期：1981-04-01 00:00:00

abstract：:The number of human genetic disorders shown to be due to defects in membrane trafficking has greatly increased during the past five years. Defects have been identified in components involved in sorting of cargo into transport carriers, vesicle budding and scission, movement of vesicles along cytoskeletal tracks, as we...

journal_title：Journal of cell science

authors： Olkkonen VM,Ikonen E

更新日期：2006-12-15 00:00:00

abstract：:Megasporogenesis in the heterosporous fern Marsilea (Hydropterideae) shows features intermediate between sporogenesis in homosporous ferns and that in heterosporous seed plants. The plastids in the dyads and young spores were associated with tubules 30-35 nm in diameter, probably a specialized form of endoplasmic reti...

journal_title：Journal of cell science

authors： Bell PR

更新日期：1981-10-01 00:00:00

abstract：:The Wnt-5a gene encodes a secreted protein that controls several normal processes during embryogenesis and development of adult tissues by as yet unknown mechanisms. Endogenous expression of Wnt-5a mRNA is known to occur in both mouse and human mammary cell lines. To investigate the biological role of Wnt-5a in the hu...

journal_title：Journal of cell science

authors： Jönsson M,Andersson T

更新日期：2001-06-01 00:00:00

abstract：:The main neuropathological features of Parkinson's disease are dopaminergic nigrostriatal neuron degeneration, and intraneuronal and intraneuritic proteinaceous inclusions named Lewy bodies and Lewy neurites, respectively, which mainly contain α-synuclein (α-syn, also known as SNCA). The neuronal phosphoprotein synaps...

journal_title：Journal of cell science

authors： Zaltieri M,Grigoletto J,Longhena F,Navarria L,Favero G,Castrezzati S,Colivicchi MA,Della Corte L,Rezzani R,Pizzi M,Benfenati F,Spillantini MG,Missale C,Spano P,Bellucci A

更新日期：2015-07-01 00:00:00

abstract：:Candida albicans is a dimorphic fungus capable of growing as a budding yeast and as a filamentous hypha. We have used the technique of immunofluorescence to study the changes in the microtubule cytoskeleton during the cell cycle in both growth forms. This approach has revealed the presence of an extensive system of mi...

journal_title：Journal of cell science

authors： Barton R,Gull K

更新日期：1988-10-01 00:00:00

abstract：:The extracellular environment and tissue architecture contribute to proper cell function and growth control. Cells growing in monolayers on standard polystyrene tissue culture plates differ in their shape, growth rate and response to external stimuli, compared with cells growing in vivo. Here, we showed that the EGFR ...

journal_title：Journal of cell science

authors： Mizushima H,Wang X,Miyamoto S,Mekada E

更新日期：2009-12-01 00:00:00

abstract：:We have investigated the role in macrophage lysosomal enzyme release of arachidonate metabolites, extracellular divalent cations and cyclic AMP (cAMP) which modulate secretion in other cell types. Lysosomal enzyme secretion induced by zymosan was accompanied by release of malondialdehyde (MDA), which is derived from a...

journal_title：Journal of cell science

authors： McMillan RM,Macintyre DE,Beesley JE,Gordon JL

更新日期：1980-08-01 00:00:00

abstract：:Multinucleated muscle fibres arise by fusion of precursor cells called myoblasts. We previously showed that CKIP-1 ectopic expression in C2C12 myoblasts increased cell fusion. In this work, we report that CKIP-1 depletion drastically impairs C2C12 myoblast fusion in vitro and in vivo during zebrafish muscle developmen...

journal_title：Journal of cell science

authors： Baas D,Caussanel-Boude S,Guiraud A,Calhabeu F,Delaune E,Pilot F,Chopin E,Machuca-Gayet I,Vernay A,Bertrand S,Rual JF,Jurdic P,Hill DE,Vidal M,Schaeffer L,Goillot E

更新日期：2012-08-15 00:00:00