Abstract：
:In adherent cells, the relevance of a physical mechanotransduction pathway provided by the perinuclear actin cap stress fibers has recently emerged. Here, we investigate the impact of a functional actin cap on the cellular adaptive response to topographical cues and uniaxial cyclic strain. Lmna-deficient fibroblasts are used as a model system because they do not develop an intact actin cap, but predominantly form a basal layer of actin stress fibers underneath the nucleus. We observe that topographical cues induce alignment in both normal and Lmna-deficient fibroblasts, suggesting that the topographical signal transmission occurs independently of the integrity of the actin cap. By contrast, in response to cyclic uniaxial strain, Lmna-deficient cells show a compromised strain avoidance response, which is completely abolished when topographical cues and uniaxial strain are applied along the same direction. These findings point to the importance of an intact and functional actin cap in mediating cellular strain avoidance.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Tamiello C,Halder M,Kamps MA,Baaijens FP,Broers JL,Bouten CVdoi
10.1242/jcs.184838subject
Has Abstractpub_date
2017-02-15 00:00:00pages
779-790issue
4eissn
0021-9533issn
1477-9137pii
jcs.184838journal_volume
130pub_type
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更新日期：2010-03-15 00:00:00
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doi：
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更新日期：2000-11-01 00:00:00
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doi：
更新日期：1996-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1242/jcs.01504
更新日期：2004-11-15 00:00:00
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更新日期：1979-02-01 00:00:00
