Disruption of microtubules uncouples budding and nuclear division in Toxoplasma gondii.

abstract：:Previous studies have shown that unactivated lymphocytes bind to CS1 peptide and that the adhesion of these cells to high endothelium is inhibited by CS1 peptide. These results suggest that lymphocyte binding occurs via recognition of the CS1-containing splice variant of fibronectin expressed on the high endothelial s...

journal_title：Journal of cell science

authors： May MJ,Entwistle G,Humphries MJ,Ager A

更新日期：1993-09-01 00:00:00

abstract：:During early stages of meiotic prophase I the nucleus undergoes considerable reorganization, including the clustering of telomeres, the release of contacts between chromosomes and the nuclear membrane, the reorganization of the nucleolus, and chromatin remodeling. Using a light squashing technique for the analysis of ...

journal_title：Journal of cell science

authors： Yang X,Timofejeva L,Ma H,Makaroff CA

更新日期：2006-09-15 00:00:00

abstract：:Transcripts containing expanded CNG repeats, which are found in several neuromuscular diseases, are not exported from the nucleus and aggregate as ribonuclear inclusions by an unknown mechanism. Using the MS2-GFP system, which tethers fluorescent proteins to a specific mRNA, we followed the dynamics of single CUG-repe...

journal_title：Journal of cell science

authors： Querido E,Gallardo F,Beaudoin M,Ménard C,Chartrand P

更新日期：2011-05-15 00:00:00

abstract：:In Rat-1 fibroblasts, v-Src causes a profound remodelling of cortical actin cytoskeleton. This transformation includes membrane ruffling, a hallmark of the leading edge in migrating cells, and results from activation of phosphoinositide 3-kinase (PI 3-kinase), phospholipase C (PLC) and phospholipase D (PLD). We theref...

journal_title：Journal of cell science

authors： Platek A,Mettlen M,Camby I,Kiss R,Amyere M,Courtoy PJ

更新日期：2004-09-15 00:00:00

abstract：:Adhesion between cells is established by the formation of specialized intercellular junctional complexes, such as desmosomes. Desmosomes contain isoforms of two members of the cadherin superfamily of cell adhesion proteins, desmocollins (Dsc) and desmogleins (Dsg), but their combinatorial roles in desmosome assembly a...

journal_title：Journal of cell science

authors： Lowndes M,Rakshit S,Shafraz O,Borghi N,Harmon RM,Green KJ,Sivasankar S,Nelson WJ

更新日期：2014-05-15 00:00:00

abstract：:The protozoan parasite Giardia intestinalis (also known as Giardia lamblia) is a major waterborne pathogen. During its life cycle, Giardia alternates between the actively growing trophozoite, which has two diploid nuclei with low levels of allelic heterozygosity, and the infectious cyst, which has four nuclei and a to...

journal_title：Journal of cell science

authors： Carpenter ML,Assaf ZJ,Gourguechon S,Cande WZ

更新日期：2012-05-15 00:00:00

abstract：:Centrioles organise centrosomes and cilia, and these organelles have an important role in many cell processes. In flies, the centriole protein Ana1 is required for the assembly of functional centrosomes and cilia. It has recently been shown that Cep135 (also known as Bld10) initially recruits Ana1 to newly formed cent...

journal_title：Journal of cell science

authors： Saurya S,Roque H,Novak ZA,Wainman A,Aydogan MG,Volanakis A,Sieber B,Pinto DM,Raff JW

更新日期：2016-07-01 00:00:00

abstract：:Key factors in the adenosine 3',5'-cyclic monophosphate (cyclic AMP) metabolic pathway are two enzymes responsible for its generation and degradation, namely, adenylate cyclase (AC) and phosphodiesterase (PDE). In LD: 12,12 (12 h light, 12 h dark), these enzymes were found to undergo bimodal, circadian variation of ac...

journal_title：Journal of cell science

authors： Tong J,Carre IA,Edmunds LN Jr

更新日期：1991-10-01 00:00:00

abstract：:Regulation of cell-cell contacts is essential for integrity of the vascular endothelium. Here, a critical role of the F-actin-binding protein drebrin in maintaining endothelial integrity is revealed under conditions mimicking vascular flow. Drebrin knockdown leads to weakening of cell-cell contacts, characterized by l...

journal_title：Journal of cell science

authors： Rehm K,Panzer L,van Vliet V,Genot E,Linder S

更新日期：2013-08-15 00:00:00

abstract：:In many cells, the endoplasmic reticulum (ER) contains segregated smooth and rough domains, but the mechanism of this segregation is unclear. Here, we used a HeLa cell line, inducibly expressing a GFP fusion protein [GFP-b(5)tail] anchored to the ER membrane, as a tool to investigate factors influencing ER organisatio...

journal_title：Journal of cell science

authors： Sprocati T,Ronchi P,Raimondi A,Francolini M,Borgese N

更新日期：2006-08-01 00:00:00

abstract：:Cells synthesize ceramides in the endoplasmic reticulum (ER) as precursors for sphingolipids to form an impermeable plasma membrane. As ceramides are engaged in apoptotic pathways, cells would need to monitor their levels closely to avoid killing themselves during sphingolipid biosynthesis. How this is accomplished re...

journal_title：Journal of cell science

authors： Tafesse FG,Vacaru AM,Bosma EF,Hermansson M,Jain A,Hilderink A,Somerharju P,Holthuis JC

更新日期：2014-01-15 00:00:00

abstract：:By operating as both a subunit of the cadherin complex and a key component of Wnt signalling, β-catenin acts as the lynchpin between cell-cell contact and transcriptional regulation of proliferation, coordinating epithelial tissue homeostasis and regeneration. The integration of multiple growth-regulatory inputs with ...

journal_title：Journal of cell science

authors： Georgopoulos NT,Kirkwood LA,Southgate J

更新日期：2014-07-01 00:00:00

abstract：:The alternative lengthening of telomeres (ALT) mechanism allows cancer cells to escape senescence and apoptosis in the absence of active telomerase. A characteristic feature of this pathway is the assembly of ALT-associated promyelocytic leukemia (PML) nuclear bodies (APBs) at telomeres. Here, we dissected the role of...

journal_title：Journal of cell science

authors： Osterwald S,Deeg KI,Chung I,Parisotto D,Wörz S,Rohr K,Erfle H,Rippe K

更新日期：2015-05-15 00:00:00

abstract：:Tumour necrosis factor receptors (TNF-Rs) and their ligands, tumour necrosis factors, are highly conserved proteins described in all metazoan phyla. They function as inducers of extrinsic apoptotic signalling and facilitate inflammation, differentiation and cell survival. TNF-Rs use distinct adaptor molecules to activ...

journal_title：Journal of cell science

authors： Steichele M,Sauermann LS,König AC,Hauck S,Böttger A

更新日期：2021-01-25 00:00:00

abstract：:Basement membranes are complex extracellular matrices contributing to the regulation of growth, migration and differentiation of many cell types. However, little is known about the mechanisms regulating the deposition and assembly of basement membrane from its constituents. We have investigated the role of extracellul...

journal_title：Journal of cell science

authors： Austria MR,Couchman JR

更新日期：1991-06-01 00:00:00

abstract：:In mammalian cells, Rho GTPases control the reorganisation of the actin cytoskeleton in response to growth factors. In the cytoplasm, the polymerisation of actin filaments and their organisation into complex architectures is orchestrated by numerous proteins which act either directly, by interacting with actin, or by ...

journal_title：Journal of cell science

authors： Dutartre H,Davoust J,Gorvel JP,Chavrier P

更新日期：1996-02-01 00:00:00

abstract：:The expression level and functional activity of estrogen receptor alpha is an important determinant of breast physiology and breast cancer treatment. However, it has been difficult to identify the signals that regulate estrogen receptor because cultured mammary epithelial cells generally do not respond to estrogenic s...

journal_title：Journal of cell science

authors： Novaro V,Roskelley CD,Bissell MJ

更新日期：2003-07-15 00:00:00

abstract：:Morphological, enzymic and antigenic data are presented regarding a human bone-marrow stromal cell line maintained for 10 months and subcultured weekly. The main characteristics are a fibroblastoid morphology, diffuse growth in collagen gels, no colony formation in soft gel media, contact inhibition of growth and conv...

journal_title：Journal of cell science

authors： Lanotte M,Allen TD,Dexter TM

更新日期：1981-08-01 00:00:00

abstract：:MicroRNAs are known to play regulatory roles in gene expression associated with cancer development. We analyzed levels of the microRNA miR-24 in patients with breast carcinoma and found that miR-24 was higher in breast carcinoma samples than in benign breast tissues. We generated constructs expressing miR-24 and studi...

journal_title：Journal of cell science

authors： Du WW,Fang L,Li M,Yang X,Liang Y,Peng C,Qian W,O'Malley YQ,Askeland RW,Sugg SL,Qian J,Lin J,Jiang Z,Yee AJ,Sefton M,Deng Z,Shan SW,Wang CH,Yang BB

更新日期：2013-03-15 00:00:00

abstract：:We have isolated mini-titin from the nematodes Ascaris lumbricoides and Caenorhabditis elegans under native conditions using a modification in the procedure to prepare this protein from insect muscle. The proteins have an apparent molecular weight of 600,000 and appear in oriented specimens as flexible thin rods with ...

journal_title：Journal of cell science

authors： Nave R,Fürst D,Vinkemeier U,Weber K

更新日期：1991-04-01 00:00:00

abstract：:The acidic carboxy-terminal regions of alpha- and beta-tubulin subunits are currently thought to be centrally involved in microtubule stability and in microtubule association with a variety of proteins (MAPs) such as MAP2 and tau proteins. Here, pure tubulin microtubules were exposed to subtilisin to produce polymers ...

journal_title：Journal of cell science

authors： Saoudi Y,Paintrand I,Multigner L,Job D

更新日期：1995-01-01 00:00:00

abstract：:Although the anti-apoptotic activity of Bcl-2 has been extensively studied, its mode of action is still incompletely understood. In the nematode Caenorhabditis elegans, 131 of 1090 somatic cells undergo programmed cell death during development. Transgenic expression of human Bcl-2 reduced cell death during nematode de...

journal_title：Journal of cell science

authors： Jabbour AM,Puryer MA,Yu JY,Lithgow T,Riffkin CD,Ashley DM,Vaux DL,Ekert PG,Hawkins CJ

更新日期：2006-06-15 00:00:00

abstract：:Protein with tau-like repeats (PTL-1) is the sole Caenorhabditis elegans homolog of tau and MAP2, which are members of the mammalian family of microtubule-associated proteins (MAPs). In mammalian neurons, tau and MAP2 are segregated, with tau being mainly localised to the axon and MAP2 mainly to the dendrite. In parti...

journal_title：Journal of cell science

authors： Chew YL,Fan X,Götz J,Nicholas HR

更新日期：2013-05-01 00:00:00

abstract：:We have isolated suppressors of the deletion allele of delta-tubulin, uni3-1, in the biflagellate green alga Chlamydomonas reinhardtii. The deletion of delta-tubulin produces cells that assemble zero, one or two flagella and have basal bodies composed primarily of doublet rather than triplet microtubules. Flagellar nu...

journal_title：Journal of cell science

authors： Fromherz S,Giddings TH Jr,Gomez-Ospina N,Dutcher SK

更新日期：2004-01-15 00:00:00

abstract：:More than 15 subunits of the integrin family of cell surface adhesion molecules have been identified. The alpha 6 beta 4 integrin has recently been identified as a component of hemidesmosomes of stratified squamous epithelium. The monoclonal antibody (mAb) 346-11A binds to the beta 4 subunit in mice. Sequence analysis...

journal_title：Journal of cell science

authors： Kennel SJ,Godfrey V,Ch'ang LY,Lankford TK,Foote LJ,Makkinje A

更新日期：1992-01-01 00:00:00

abstract：:Epithelial junctions depend on intercellular interactions between β(1) subunits of the Na(+)/K(+)-ATPase molecules of neighboring cells. The interaction between dog and rat subunits is less effective than the interaction between two dog β(1) subunits, indicating the importance of species-specific regions for β(1)-β(1)...

journal_title：Journal of cell science

authors： Tokhtaeva E,Sachs G,Sun H,Dada LA,Sznajder JI,Vagin O

更新日期：2012-03-15 00:00:00

abstract：:In contrast to the highly dynamic actin cytoskeleton in non-muscle cells, actin filaments in muscle sarcomeres are thought to be relatively stable and undergo dynamics only at their ends. However, many proteins that promote rapid actin dynamics are also expressed in striated muscles. We show that a subset of actin fil...

journal_title：Journal of cell science

authors： Skwarek-Maruszewska A,Hotulainen P,Mattila PK,Lappalainen P

更新日期：2009-06-15 00:00:00

abstract：:In this study we describe DNA-RNA complexes in matrix DNA of Friend cells. The presence of such unusual structures is confirmed by the following evidence. When a preparation of matrix DNA is electrophoresed in agarose an RNA component always migrates together with DNA. There should be a close interaction between DNA a...

journal_title：Journal of cell science

authors： Patriotis C,Andreeva M,Pascaleva M,Ivanov V,Djondjurov L

更新日期：1990-04-01 00:00:00

abstract：:Cyclin E is a regulatory subunit of the cdc2-related protein kinase cdk2, which is activated shortly before S-phase entry, thus defining it as a G1 cyclin. We report here the existence of a 43 kDa splice variant of human cyclin E, termed cyclin Es, which lacks 49 amino acids within the cyclin box compared to the known...

journal_title：Journal of cell science

authors： Sewing A,Rönicke V,Bürger C,Funk M,Müller R

更新日期：1994-02-01 00:00:00

abstract：:In adipocytes and muscle cells, the GLUT4 glucose transporter isoform is present in intracellular vesicles which continuously recycle between an intracytoplasmic location and the plasma membrane. It is not clear whether the GLUT4-vesicles represent a specific kind of vesicle or resemble typical secretory granules or s...

journal_title：Journal of cell science

authors： Thorens B,Roth J

更新日期：1996-06-01 00:00:00