Functional consequences in yeast of single-residue alterations in a consensus calmodulin.

abstract：:A cDNA encoding rat CaBP1 has been isolated and sequenced. The deduced polypeptide chain consists of 440 amino acids including two internal thioredoxin-like domains and a C-terminal KDEL retention/retrieval signal. Regarding the high degree of identity to the hamster protein P5, CaBP1 is considered to be the homologou...

journal_title：Journal of cell science

authors： Füllekrug J,Sönnichsen B,Wünsch U,Arseven K,Nguyen Van P,Söling HD,Mieskes G

更新日期：1994-10-01 00:00:00

abstract：:Podosomes are actin-rich adhesion and invasion structures. Especially in macrophages, podosomes exist in two subpopulations, large precursors at the cell periphery and smaller podosomes (successors) in the cell interior. To date, the mechanisms that differentially regulate these subpopulations are largely unknown. Her...

journal_title：Journal of cell science

authors： Bhuwania R,Cornfine S,Fang Z,Krüger M,Luna EJ,Linder S

更新日期：2012-05-01 00:00:00

abstract：:Mammalian cytochrome P-450s in the CYP1A gene family catalyse the oxidation of a wide range of drugs and foreign compounds resulting in their excretion. These enzymes are highly inducible by a range of compounds, including polycyclic aromatic hydrocarbons such as 3-methylcholanthrene (3-MC) and dioxins. Analysis of th...

journal_title：Journal of cell science

authors： Campbell SJ,Carlotti F,Hall PA,Clark AJ,Wolf CR

更新日期：1996-11-01 00:00:00

abstract：:Endo180 was previously characterized as a novel, cell type specific, recycling transmembrane glycoprotein. This manuscript describes the isolation of a full length human Endo180 cDNA clone which was shown to encode a fourth member of a family of proteins comprising the macrophage mannose receptor, the phospholipase A(...

journal_title：Journal of cell science

authors： Sheikh H,Yarwood H,Ashworth A,Isacke CM

更新日期：2000-03-01 00:00:00

abstract：:Cell surface glycans are believed to play a role in tumour invasion and metastasis. Yet, we have previously shown that the inhibitors of N-linked glycan processing swainsonine (SW) and 1-deoxynojirimycin (dNM) did not prevent invasion of chick heart fragments by MO4 murine fibrosarcoma cells in organ culture. We now p...

journal_title：Journal of cell science

authors： Bruyneel EA,De Mets M,Dragonetti CH,Hooghe RJ,Di Virgilio S,Mareel MM

更新日期：1990-02-01 00:00:00

abstract：:Vertebrate nuclear lamins form a multigene family with developmentally controlled expression. In contrast, invertebrates have long been thought to contain only a single lamin, which in Drosophila is the well-characterized lamin Dm0. Recently, however, a Drosophila cDNA clone (pG-IF) has been identified that codes for ...

journal_title：Journal of cell science

authors： Riemer D,Stuurman N,Berrios M,Hunter C,Fisher PA,Weber K

更新日期：1995-10-01 00:00:00

abstract：:During vertebrate gastrulation, a well-orchestrated series of morphogenetic changes leads to the formation of the three germ layers: the ectoderm, mesoderm and endoderm. The analysis of gene expression patterns during gastrulation has been central to the identification of genes involved in germ layer formation. Howeve...

journal_title：Journal of cell science

authors： Link V,Carvalho L,Castanon I,Stockinger P,Shevchenko A,Heisenberg CP

更新日期：2006-05-15 00:00:00

abstract：:Inhibitory and excitatory neurons exhibit distinct patterns of development in the mammalian cerebral cortex. The morphological development of inhibitory and excitatory neurons derived from fetal rat cerebral cortex has now been compared in vitro. Inhibitory neurons were identified by immunofluorescence staining with a...

journal_title：Journal of cell science

authors： Hayashi K,Kawai-Hirai R,Harada A,Takata K

更新日期：2003-11-01 00:00:00

abstract：:Mechanical cues are essential for the regulation of cell and tissue physiology. Hence, it has become an utmost necessity for cell biologists to account for those mechanical parameters when investigating biological processes and they need devices to manipulate cells accordingly. Here, we report a simple mechanical cell...

journal_title：Journal of cell science

authors： Boulter E,Tissot FS,Dilly J,Pisano S,Féral CC

更新日期：2020-01-06 00:00:00

abstract：:RIF1 controls both DNA replication timing and the DNA double-strand break (DSB) repair pathway to maintain genome integrity. However, it remains unclear how RIF1 links these two processes following exposure to ionizing radiation (IR). Here, we show that inhibition of homologous recombination repair (HRR) by RIF1 occur...

journal_title：Journal of cell science

authors： Saito Y,Kobayashi J,Kanemaki MT,Komatsu K

更新日期：2020-06-22 00:00:00

abstract：:Export out of the endoplasmic reticulum (ER) involves the Sar1 and COPII machinery acting at ER exit sites (ERES). Whether and how cargo proteins are recruited upstream of Sar1 and COPII is unclear. Two models are conceivable, a recruitment model where cargo is actively transported through a transport factor and hande...

journal_title：Journal of cell science

authors： Yonemura Y,Li X,Müller K,Krämer A,Atigbire P,Mentrup T,Feuerhake T,Kroll T,Shomron O,Nohl R,Arndt HD,Hoischen C,Hemmerich P,Hirschberg K,Kaether C

更新日期：2016-10-15 00:00:00

abstract：:The establishment of the junctional complex in epithelial cells requires the presence of extracellular calcium, and is controlled by a network of reactions involving G-proteins, phospholipase C and protein kinase C. Since potential candidates for phosphorylation are the tight junction associated proteins ZO1, ZO2 and ...

journal_title：Journal of cell science

authors： Perez-Moreno M,Avila A,Islas S,Sanchez S,González-Mariscal L

更新日期：1998-12-01 00:00:00

abstract：:Major histocompatibility complex class I (MHC-I) molecules signal infection or transformation by engaging receptors on T lymphocytes. The spatial organization of MHC-I on the plasma membranes is important for this engagement. We and others have shown that MHC-I molecules, like other membrane proteins, are not uniforml...

journal_title：Journal of cell science

authors： Blumenthal D,Edidin M,Gheber LA

更新日期：2016-09-01 00:00:00

abstract：:Endocytic trafficking is regulated by ubiquitylation (also known as ubiquitination) of cargoes and endocytic machineries. The role of ubiquitylation in lysosomal delivery has been well documented, but its role in the recycling pathway is largely unknown. Here, we report that the ubiquitin (Ub) ligase RFFL regulates ub...

journal_title：Journal of cell science

authors： Sakai R,Fukuda R,Unida S,Aki M,Ono Y,Endo A,Kusumi S,Koga D,Fukushima T,Komada M,Okiyoneda T

更新日期：2019-02-07 00:00:00

abstract：:Using a polyclonal antibody against purified zymogen granule membrane components from rat pancreas a cDNA coding for the 29 kDa protein (ZG29p) was identified by immunoscreening of a hormonally stimulated pancreas cDNA library. Western blot analysis suggests that ZG29p is a pancreas-specific protein and immunofluoresc...

journal_title：Journal of cell science

authors： Kleene R,Zdzieblo J,Wege K,Kern HF

更新日期：1999-08-01 00:00:00

abstract：:When tumour cells are fused with normal ones, malignancy is suppressed. It has been shown that this suppression is associated with the imposition on the hybrid cell of the terminal differentiation programme of the normal parent cell. We report here the consequences of imposing the synthesis of keratin 1 and keratin 10...

journal_title：Journal of cell science

authors： Harris H,Rawlins J,Sharps J

更新日期：1996-09-01 00:00:00

abstract：:The family of integrin transmembrane receptors is essential for the normal function of multicellular organisms by facilitating cell-extracellular matrix adhesion. The vitronectin-binding integrin αVβ5 localizes to focal adhesions (FAs) as well as poorly characterized flat clathrin lattices (FCLs). Here, we show that, ...

journal_title：Journal of cell science

authors： Zuidema A,Wang W,Kreft M,Te Molder L,Hoekman L,Bleijerveld OB,Nahidiazar L,Janssen H,Sonnenberg A

更新日期：2018-11-05 00:00:00

abstract：:Low-density lipoprotein (LDL) deposition, aggregation and retention in the endothelial sub-intima are critical initiating events during atherosclerosis. Macrophages digest aggregated LDL (agLDL) through a process called exophagy. High-density lipoprotein (HDL) plays an atheroprotective role, but studies attempting to ...

journal_title：Journal of cell science

authors： Singh RK,Lund FW,Haka AS,Maxfield FR

更新日期：2019-12-02 00:00:00

abstract：:The forkhead transcription factor FOXN1 is required for normal cutaneous and thymic epithelial development. Mutations in FOXN1 give rise to the nude phenotype in mice, rats and man. However, the genes that are regulated by FOXN1 are unknown. To investigate FOXN1 function we expressed an inducible form of the protein, ...

journal_title：Journal of cell science

authors： Janes SM,Ofstad TA,Campbell DH,Watt FM,Prowse DM

更新日期：2004-08-15 00:00:00

abstract：:PML nuclear bodies (NBs) are involved in the regulation of key nuclear pathways but their biochemical function in nuclear metabolism is unknown. In this study PML NB assembly dynamics were assessed by live cell imaging and mathematic modeling of its major component parts. We show that all six nuclear PML isoforms exhi...

journal_title：Journal of cell science

authors： Weidtkamp-Peters S,Lenser T,Negorev D,Gerstner N,Hofmann TG,Schwanitz G,Hoischen C,Maul G,Dittrich P,Hemmerich P

更新日期：2008-08-15 00:00:00

abstract：:The interplay between pathogen-encoded virulence factors and host cell signaling networks is critical for both the establishment and clearance of microbial infections. Yersinia uptake into host cells serves as an in vitro model for exploring how host cells respond to Yersinia adherence. In this study, we provide insig...

journal_title：Journal of cell science

authors： Bruce-Staskal PJ,Weidow CL,Gibson JJ,Bouton AH

更新日期：2002-07-01 00:00:00

abstract：:Genomes exist in vivo as complex physical structures, and their functional output (i.e. the gene expression profile of a cell) is related to their spatial organization inside the nucleus as well as to local chromatin status. Chromatin modifications and chromosome conformation are distinct in different tissues and cell...

journal_title：Journal of cell science

authors： Naumova N,Dekker J

更新日期：2010-06-15 00:00:00

abstract：:The monoclonal antibody AGF2.3 identifies a nuclear envelope protein that is restricted to certain cell types. In particular, this antigen shows a reduced level of expression during haemopoietic cell maturation. In this study, we have examined the relationship of this protein to known nuclear envelope proteins that ha...

journal_title：Journal of cell science

authors： Lord JM,Thick JA,Bunce CM,Taylor AM,Gallimore PH,Mills D,Brown G

更新日期：1988-06-01 00:00:00

abstract：:During animal development, cells need to sense and adapt to mechanical forces from their environment. Ultimately, these forces are transduced through the actomyosin cortex. How the cortex simultaneously responds to and creates forces during cytokinesis is not well understood. Here we show that, under mechanical stress...

journal_title：Journal of cell science

authors： Singh D,Odedra D,Dutta P,Pohl C

更新日期：2019-10-09 00:00:00

abstract：:Prox1 plays pivotal roles during embryonic lymphatic development and maintenance of adult lymphatic systems by modulating the expression of various lymphatic endothelial cell (LEC) markers, such as vascular endothelial growth factor receptor 3 (VEGFR3). However, the molecular mechanisms by which Prox1 transactivates i...

journal_title：Journal of cell science

authors： Yoshimatsu Y,Yamazaki T,Mihira H,Itoh T,Suehiro J,Yuki K,Harada K,Morikawa M,Iwata C,Minami T,Morishita Y,Kodama T,Miyazono K,Watabe T

更新日期：2011-08-15 00:00:00

abstract：:The contractile vacuole complex (CVC) of Trypanosoma cruzi, the etiologic agent of Chagas disease, collects and expels excess water as a mechanism of regulatory volume decrease after hyposmotic stress; it also has a role in cell shrinking after hyperosmotic stress. Here, we report that, in addition to its role in osmo...

journal_title：Journal of cell science

authors： Niyogi S,Jimenez V,Girard-Dias W,de Souza W,Miranda K,Docampo R

更新日期：2015-06-15 00:00:00

abstract：:Syntaxins are thought to participate in the specific interactions between vesicles and acceptor membranes in intracellular protein trafficking. VAM3 of Saccharomyces cerevisiae encodes a 33 kDa protein (Vam3p) with a hydrophobic transmembrane segment at its C terminus. Vam3p has structural similarities to syntaxins of...

journal_title：Journal of cell science

authors： Wada Y,Nakamura N,Ohsumi Y,Hirata A

更新日期：1997-06-01 00:00:00

abstract：:Our previous studies of PAWS1 (protein associated with SMAD1; also known as FAM83G) have suggested that this molecule has roles beyond BMP signalling. To investigate these roles, we have used CRISPR/Cas9 to generate PAWS1-knockout U2OS osteosarcoma cells. Here, we show that PAWS1 plays a role in the regulation of the ...

journal_title：Journal of cell science

authors： Cummins TD,Wu KZL,Bozatzi P,Dingwell KS,Macartney TJ,Wood NT,Varghese J,Gourlay R,Campbell DG,Prescott A,Griffis E,Smith JC,Sapkota GP

更新日期：2018-01-10 00:00:00

abstract：:p120-catenin is an E-cadherin-associated protein that modulates E-cadherin function and stability. We describe here that p120-catenin is required for Wnt pathway signaling. p120-catenin binds and is phosphorylated by CK1ε in response to Wnt3a. p120-catenin also associates to the Wnt co-receptor LRP5/6, an interaction ...

journal_title：Journal of cell science

authors： Casagolda D,Del Valle-Pérez B,Valls G,Lugilde E,Vinyoles M,Casado-Vela J,Solanas G,Batlle E,Reynolds AB,Casal JI,de Herreros AG,Duñach M

更新日期：2010-08-01 00:00:00

abstract：:Cell migration is a complex process requiring density and rigidity sensing of the microenvironment to adapt cell migratory speed through focal adhesion and actin cytoskeleton regulation. ICAP-1 (also known as ITGB1BP1), a β1 integrin partner, is essential for ensuring integrin activation cycle and focal adhesion forma...

journal_title：Journal of cell science

authors： Bouin AP,Kyumurkov A,Régent-Kloeckner M,Ribba AS,Faurobert E,Fournier HN,Bourrin-Reynard I,Manet-Dupé S,Oddou C,Balland M,Planus E,Albiges-Rizo C

更新日期：2017-02-01 00:00:00