Abstract：
:Directed axonal growth depends on correct coordination of the actin and microtubule cytoskeleton in the growth cone. However, despite the relatively large number of proteins implicated in actin-microtubule crosstalk, the mechanisms whereby actin polymerization is coupled to microtubule stabilization and advancement in the peripheral growth cone remained largely unclear. Here, we identified the formin Dishevelled-associated activator of morphogenesis (DAAM) as a novel factor playing a role in concerted regulation of actin and microtubule remodeling in Drosophilamelanogaster primary neurons. In vitro, DAAM binds to F-actin as well as to microtubules and has the ability to crosslink the two filament systems. Accordingly, DAAM associates with the neuronal cytoskeleton, and a significant fraction of DAAM accumulates at places where the actin filaments overlap with that of microtubules. Loss of DAAM affects growth cone and microtubule morphology, and several aspects of microtubule dynamics; and biochemical and cellular assays revealed a microtubule stabilization activity and binding to the microtubule tip protein EB1. Together, these data suggest that, besides operating as an actin assembly factor, DAAM is involved in linking actin remodeling in filopodia to microtubule stabilization during axonal growth.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Szikora S,Földi I,Tóth K,Migh E,Vig A,Bugyi B,Maléth J,Hegyi P,Kaltenecker P,Sanchez-Soriano N,Mihály Jdoi
10.1242/jcs.203455subject
Has Abstractpub_date
2017-08-01 00:00:00pages
2506-2519issue
15eissn
0021-9533issn
1477-9137pii
jcs.203455journal_volume
130pub_type
杂志文章abstract：:The IGF-II mRNA-binding proteins (IMPs), which are composed of two RNA recognition motifs, (RRM) and four hnRNP K homology (KH) domains, have been implicated in subcytoplasmic localization of mRNAs during embryogenesis. The IMP family originated via two gene duplications before the divergence of vertebrates, and IMP h...
journal_title：Journal of cell science
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更新日期：2002-05-15 00:00:00
abstract：:The post-translational modifications of the G protein of vesicular stomatitis virus, described in the preceding paper, indicate that its transport is arrested by carbonylcyanide m-chlorophenylhydrazone (CCCP) in or near the trans-Golgi. Immunofluorescence microscopy of BHK-21 cells infected with vesicular stomatitis v...
journal_title：Journal of cell science
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doi：
更新日期：1989-04-01 00:00:00
abstract：:The fractionation of human liver mitochondria into inner membrane, outer membrane and matrix material is reported. Compared with rat, human liver mitochondria are more fragile. Fractionation can be achieved in only 2 steps, a digitonin treatment for removal of the outer membrane and centrifugation of the inner membran...
journal_title：Journal of cell science
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更新日期：1979-02-01 00:00:00
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更新日期：2000-08-01 00:00:00
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journal_title：Journal of cell science
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更新日期：2001-10-01 00:00:00
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更新日期：2017-02-01 00:00:00
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doi：
更新日期：2001-07-01 00:00:00
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更新日期：2007-09-15 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：2000-06-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1996-03-01 00:00:00
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journal_title：Journal of cell science
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2004-11-15 00:00:00
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journal_title：Journal of cell science
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更新日期：2016-10-15 00:00:00
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更新日期：2012-09-01 00:00:00
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doi：
更新日期：1991-05-01 00:00:00
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更新日期：1988-08-01 00:00:00
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更新日期：2000-01-01 00:00:00
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更新日期：1991-10-01 00:00:00
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journal_title：Journal of cell science
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更新日期：1996-06-01 00:00:00
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