Chondrons from articular cartilage: I. Immunolocalization of type VI collagen in the pericellular capsule of isolated canine tibial chondrons.

abstract：:Using a genome-wide technical knockout, we isolated a newly identified set of GRIM (genes associated with retinoid-interferon-induced mortality) genes; GRIM genes mediate IFN- and retinoic-acid (RA)-induced cell death. Here, we describe the isolation and characterization of one such gene, GRIM-1. Three proteins, with ...

journal_title：Journal of cell science

authors： Hofmann ER,Nallar SC,Lin L,D'Cunha J,Lindner DJ,Weihua X,Kalvakolanu DV

更新日期：2010-08-15 00:00:00

abstract：:Multinucleated muscle fibres arise by fusion of precursor cells called myoblasts. We previously showed that CKIP-1 ectopic expression in C2C12 myoblasts increased cell fusion. In this work, we report that CKIP-1 depletion drastically impairs C2C12 myoblast fusion in vitro and in vivo during zebrafish muscle developmen...

journal_title：Journal of cell science

authors： Baas D,Caussanel-Boude S,Guiraud A,Calhabeu F,Delaune E,Pilot F,Chopin E,Machuca-Gayet I,Vernay A,Bertrand S,Rual JF,Jurdic P,Hill DE,Vidal M,Schaeffer L,Goillot E

更新日期：2012-08-15 00:00:00

abstract：:end13-1 was isolated in a screen for endocytosis mutants and has been shown to have a post-internalisation defect in endocytic transport as well as a defect in vacuolar protein sorting (Vps(-) phenotype), leading to secretion of newly synthesised vacuolar proteins. Here we demonstrate that END13 is identical to VPS4, ...

journal_title：Journal of cell science

authors： Zahn R,Stevenson BJ,Schröder-Köhne S,Zanolari B,Riezman H,Munn AL

更新日期：2001-05-01 00:00:00

abstract：:The role of basal epithelial cells in prostatic function, development and carcinogenesis is unknown. The ability of basal prostatic epithelial cells to acquire a luminal phenotype was explored in vitro using the NRP-152 rat dorsal-lateral prostate epithelial cell line as a model system. NRP-152, which was spontaneousl...

journal_title：Journal of cell science

authors： Danielpour D

更新日期：1999-01-01 00:00:00

abstract：:Phosphatidylinositol-3-phosphate [PtdIns(3)P] regulates endocytic and autophagic membrane traffic. In order to understand the downstream effects of PtdIns(3)P in these processes, it is important to identify PtdIns(3)P-binding proteins, many of which contain FYVE zinc-finger domains. Here, we describe a novel giant FYV...

journal_title：Journal of cell science

authors： Simonsen A,Birkeland HC,Gillooly DJ,Mizushima N,Kuma A,Yoshimori T,Slagsvold T,Brech A,Stenmark H

更新日期：2004-08-15 00:00:00

abstract：:A new mouse model has been developed to study the localisation and trafficking of the glucose transporter GLUT4 in muscle. The mouse line has specific expression of a GFP and HA-epitope-tagged version of GLUT4 under the control of a muscle-specific promoter. The exofacial HA-tag has enabled fluorescent labelling of on...

journal_title：Journal of cell science

authors： Fazakerley DJ,Lawrence SP,Lizunov VA,Cushman SW,Holman GD

更新日期：2009-03-01 00:00:00

abstract：:Hemidesmosomes are epithelial-specific attachment structures that maintain tissue integrity and resist tension. Despite their importance, how hemidesmosomes are regulated at the post-transcriptional level is poorly understood. Caenorhabditiselegans hemidesmosomes (CeHDs) have a similar structure and composition to the...

journal_title：Journal of cell science

authors： Fu R,Zhu Y,Jiang X,Li Y,Zhu M,Dong M,Huang Z,Wang C,Labouesse M,Zhang H

更新日期：2018-06-11 00:00:00

abstract：:A comprehensive analysis of the molecular network of cellular factors establishing and maintaining pluripotency as well as self renewal of pluripotent stem cells is key for further progress in understanding basic stem cell biology. Nanog is necessary for the natural induction of pluripotency in early mammalian develop...

journal_title：Journal of cell science

authors： Münst B,Thier MC,Winnemöller D,Helfen M,Thummer RP,Edenhofer F

更新日期：2016-03-01 00:00:00

abstract：:The molecular motor dynein is essential for mitotic spindle orientation, which defines the axis of cell division. The light intermediate chain subunits, LIC1 and LIC2, define biochemically and functionally distinct vertebrate dynein complexes, with LIC2-dynein playing a crucial role in ensuring spindle orientation. We...

journal_title：Journal of cell science

authors： Sharma A,Dagar S,Mylavarapu SVS

更新日期：2020-06-23 00:00:00

abstract：:Drosophila Stardust (Sdt), a member of the MAGUK family of scaffolding proteins, is a constituent of the evolutionarily conserved Crumbs-Stardust (Crb-Sdt) complex that controls epithelial cell polarity in the embryo and morphogenesis of photoreceptor cells. Although apical localisation is a hallmark of the complex in...

journal_title：Journal of cell science

authors： Bulgakova NA,Kempkens O,Knust E

更新日期：2008-06-15 00:00:00

abstract：:Vaccinia virus B1 kinase plays a key role in viral DNA replication. The homologous mammalian vaccinia-related kinases (VRKs) are also implicated in the regulation of DNA replication, although direct evidence remains elusive. Here we show that VRK1 regulates cell cycle progression in the DNA replication period by induc...

journal_title：Journal of cell science

authors： Kang TH,Park DY,Kim W,Kim KT

更新日期：2008-09-15 00:00:00

abstract：:PINCH1 is composed of 5 LIM domains, binds integrin-linked kinase (ILK) and locates to integrin-mediated adhesion sites. In order to investigate PINCH1 function we generated mice and embryonic stem (ES) cell-derived embryoid bodies (EBs) lacking the PINCH1 gene. Similar to mice lacking beta1 integrin or Ilk, loss of P...

journal_title：Journal of cell science

authors： Li S,Bordoy R,Stanchi F,Moser M,Braun A,Kudlacek O,Wewer UM,Yurchenco PD,Fässler R

更新日期：2005-07-01 00:00:00

abstract：:The 16 kDa proteolipid (subunit c) of the eukaryotic vacuolar H(+)-ATPase (V-ATPase) is closely related to the ductin polypeptide that forms the connexon channel of gap junctions in the crustacean Nephrops norvegicus. Here we show that the major protein component of Manduca sexta gap junction preparations is a 16 kDa ...

journal_title：Journal of cell science

authors： Finbow ME,Goodwin SF,Meagher L,Lane NJ,Keen J,Findlay JB,Kaiser K

更新日期：1994-07-01 00:00:00

abstract：:Eukaryotic cells have developed complex machineries to distribute proteins and lipids from the Golgi complex. Contrary to what has originally been postulated, delivery of proteins to the cell surface is not a simple bulk flow process but involves sorting into distinct pathways from the trans-Golgi network. Here we des...

journal_title：Journal of cell science

authors： Keller P,Simons K

更新日期：1997-12-01 00:00:00

abstract：:In adipocytes and muscle cells, the GLUT4 glucose transporter isoform is present in intracellular vesicles which continuously recycle between an intracytoplasmic location and the plasma membrane. It is not clear whether the GLUT4-vesicles represent a specific kind of vesicle or resemble typical secretory granules or s...

journal_title：Journal of cell science

authors： Thorens B,Roth J

更新日期：1996-06-01 00:00:00

abstract：:MIP and MP70 are putative gap junction components in the plasma membranes of the mammalian lens fibre cells. We show now that MP70 can be solubilized separately from MIP in mild detergent solutions, and that this treatment results in the dissociation of the fibre gap junctions. Solubilized MP70 was isolated as 16.9 S ...

journal_title：Journal of cell science

authors： Kistler J,Bullivant S

更新日期：1988-11-01 00:00:00

abstract：:Neutrophil migration is vital for immunity and precedes effector functions such as pathogen killing. Here, we report that this process is regulated by the Rab27a GTPase, a protein known to control granule exocytosis. Rab27a-deficient (Rab27a KO) neutrophils exhibit migration defects in vitro and in vivo, and live-cell...

journal_title：Journal of cell science

authors： Singh RK,Liao W,Tracey-White D,Recchi C,Tolmachova T,Rankin SM,Hume AN,Seabra MC

更新日期：2012-04-01 00:00:00

abstract：:The tandemly organised ribosomal DNA (rDNA) repeats are transcribed by a dedicated RNA polymerase in a specialised nuclear compartment, the nucleolus. There appears to be an intimate link between the maintenance of nucleolar structure and the presence of heterochromatic chromatin domains. This is particularly evident ...

journal_title：Journal of cell science

authors： Akhmanova A,Verkerk T,Langeveld A,Grosveld F,Galjart N

更新日期：2000-12-01 00:00:00

abstract：:A growing number of proteins originally found in endocytic structures of the plasma membrane appear to be able to traffic into the nucleus, but the cellular function of this translocation remains unclear. We have found that beta-arrestin2, which typically shows a cytoplasmic localization owing to constitutive nuclear ...

journal_title：Journal of cell science

authors： Neuhaus EM,Mashukova A,Barbour J,Wolters D,Hatt H

更新日期：2006-08-01 00:00:00

abstract：:Notwithstanding the fact that adult bone marrow cell engraftment to epithelial organs seems a somewhat uncommon event, there is no doubt it does occur, and under appropriate conditions of a strong and positive selection pressure these cells will expand clonally and make a significant contribution to tissue replacement...

journal_title：Journal of cell science

authors： Alison MR,Poulsom R,Otto WR,Vig P,Brittan M,Direkze NC,Preston SL,Wright NA

更新日期：2003-02-15 00:00:00

abstract：:Sequence similarity between the Drosophila segment polarity protein Armadillo and the vertebrate adherens junction protein beta-catenin raised the possibility that adherens junctions function in transduction of intercellular signals like that mediated by Wingless/Wnt-1. To substantiate the sequence similarity, propert...

journal_title：Journal of cell science

authors： Peifer M

更新日期：1993-08-01 00:00:00

abstract：:During the interphase to metaphase transition, microtubules are destabilized by a cdc2 kinase-dependent phosphorylation event. This destabilization is due to a dramatic increase in the rate at which each growing microtubule starts to shrink (catastrophe rate). In principle, this could be brought about by lowering the ...

journal_title：Journal of cell science

authors： Faruki S,Dorée M,Karsenti E

更新日期：1992-01-01 00:00:00

abstract：:Interferon regulatory factor 6 (Irf6) regulates keratinocyte proliferation and differentiation. In this study, we tested the hypothesis that Irf6 regulates cellular migration and adhesion. Irf6-deficient embryos at 10.5 days post-conception failed to close their wound compared with wild-type embryos. In vitro, Irf6-de...

journal_title：Journal of cell science

authors： Biggs LC,Naridze RL,DeMali KA,Lusche DF,Kuhl S,Soll DR,Schutte BC,Dunnwald M

更新日期：2014-07-01 00:00:00

abstract：:Compartmentalization of membrane transport and signaling processes is of pivotal importance to eukaryotic cell function. While plasma membrane compartmentalization and dynamics are well known to depend on the scaffolding function of septin GTPases, the roles of septins at intracellular membranes have remained largely ...

journal_title：Journal of cell science

authors： Song K,Gras C,Capin G,Gimber N,Lehmann M,Mohd S,Puchkov D,Rödiger M,Wilhelmi I,Daumke O,Schmoranzer J,Schürmann A,Krauss M

更新日期：2019-02-01 00:00:00

abstract：:Dynamin, a microtubule-activated GTPase, has recently been identified as the product of the shibire gene in Drosophila. shi(ts) mutants are defective in synaptic vesicle recycling, which leads to rapid and reversible temperature-sensitive paralysis. In the present study, results from RNase protection assays and analys...

journal_title：Journal of cell science

authors： Chen MS,Burgess CC,Vallee RB,Wadsworth SC

更新日期：1992-11-01 00:00:00

abstract：:The key read-out of Wnt signalling is a change in the transcriptional profile of the cell, which is driven by β-catenin. β-catenin levels are normally kept low by a phosphorylation event that is mediated by glycogen synthase kinase 3 (GSK3, α- and β-isoforms), which targets β-catenin for ubiquitylation and proteasomal...

journal_title：Journal of cell science

authors： Metcalfe C,Bienz M

更新日期：2011-11-01 00:00:00

abstract：:Barrier junctions prevent pathogen invasion and restrict paracellular leakage across epithelial sheets. To understand how one barrier junction, the septate junction (SJ), is regulated in vivo, we used fluorescence recovery after photobleaching (FRAP) to examine SJ protein dynamics in Drosophila. Most SJ-associated pro...

journal_title：Journal of cell science

authors： Oshima K,Fehon RG

更新日期：2011-08-15 00:00:00

abstract：:Concomitant expression of mutant p53 and oncogenic Ras, leading to cellular transformation, is well documented. However, the mechanisms by which the various mutant p53 categories cooperate with Ras remain largely obscure. From this study we suggest that different mutant p53 categories cooperate with H-Ras in different...

journal_title：Journal of cell science

authors： Solomon H,Buganim Y,Kogan-Sakin I,Pomeraniec L,Assia Y,Madar S,Goldstein I,Brosh R,Kalo E,Beatus T,Goldfinger N,Rotter V

更新日期：2012-07-01 00:00:00

abstract：:TANGLED (TAN) is the founding member of a family of plant-specific proteins required for correct orientation of the division plane. Arabidopsis thaliana TAN is localized before prophase until the end of cytokinesis at the cortical division site (CDS), where it appears to help guide the cytokinetic apparatus towards th...

journal_title：Journal of cell science

authors： Rasmussen CG,Sun B,Smith LG

更新日期：2011-01-15 00:00:00

abstract：:We have evaluated a series of laminarin sulphates with different degrees of sulphation (0.3-2.3) as antagonists of basic fibroblast growth factor (bFGF) and as inhibitors of the bFGF-dependent endothelial cell line FBHE. Inhibition of binding of bFGF by the laminarin sulphates increased with increasing degree of sulph...

journal_title：Journal of cell science

authors： Hoffman R,Paper DH,Donaldson J,Alban S,Franz G

更新日期：1995-11-01 00:00:00