Expression of prelamin A but not mature lamin A confers sensitivity of DNA biosynthesis to lovastatin on F9 teratocarcinoma cells.

abstract：:Neural progenitors in the vertebrate nervous system are fully polarized epithelial cells, with intercellular junctions at the apical region. These progenitor cells remain within the neuroepithelium throughout neurogenesis, and will ultimately give rise to all the neurons in the mature nervous system. We have addressed...

journal_title：Journal of cell science

authors： Afonso C,Henrique D

更新日期：2006-10-15 00:00:00

abstract：:In many cells, the endoplasmic reticulum (ER) contains segregated smooth and rough domains, but the mechanism of this segregation is unclear. Here, we used a HeLa cell line, inducibly expressing a GFP fusion protein [GFP-b(5)tail] anchored to the ER membrane, as a tool to investigate factors influencing ER organisatio...

journal_title：Journal of cell science

authors： Sprocati T,Ronchi P,Raimondi A,Francolini M,Borgese N

更新日期：2006-08-01 00:00:00

abstract：:Phosphoinositides regulate myriad cellular processes, acting as potent signaling molecules in conserved signaling pathways and as organelle gatekeepers that recruit effector proteins to membranes. Phosphoinositide-generating enzymes have been studied extensively in yeast and cultured cells, yet their roles in animal d...

journal_title：Journal of cell science

authors： Tan J,Oh K,Burgess J,Hipfner DR,Brill JA

更新日期：2014-03-01 00:00:00

abstract：:Epidermal growth factor receptor (EGFR) ligands undergo a complex series of processing events during their maturation to active signaling proteins. Like its mammalian homologs, the predominant Drosophila EGFR ligand Spitz is produced as a transmembrane pro-protein. In the secretory pathway, Spitz is cleaved within its...

journal_title：Journal of cell science

authors： Steinhauer J,Liu HH,Miller E,Treisman JE

更新日期：2013-10-01 00:00:00

abstract：:The nimO predicted protein of Aspergillus nidulans is related structurally and functionally to Dbf4p, the regulatory subunit of Cdc7p kinase in budding yeast. nimOp and Dbf4p are most similar in their C-termini, which contain a PEST motif and a novel, short-looped Cys2-His2 zinc finger-like motif. DNA labelling and re...

journal_title：Journal of cell science

authors： James SW,Bullock KA,Gygax SE,Kraynack BA,Matura RA,MacLeod JA,McNeal KK,Prasauckas KA,Scacheri PC,Shenefiel HL,Tobin HM,Wade SD

更新日期：1999-05-01 00:00:00

abstract：:p120-catenin is an E-cadherin-associated protein that modulates E-cadherin function and stability. We describe here that p120-catenin is required for Wnt pathway signaling. p120-catenin binds and is phosphorylated by CK1ε in response to Wnt3a. p120-catenin also associates to the Wnt co-receptor LRP5/6, an interaction ...

journal_title：Journal of cell science

authors： Casagolda D,Del Valle-Pérez B,Valls G,Lugilde E,Vinyoles M,Casado-Vela J,Solanas G,Batlle E,Reynolds AB,Casal JI,de Herreros AG,Duñach M

更新日期：2010-08-01 00:00:00

abstract：:Mitochondrial DNA (mtDNA) replication and transcription are crucial for cell function, but these processes are poorly understood at the single-cell level. We describe a novel fluorescence in situ hybridization protocol, called mTRIP (mitochondrial transcription and replication imaging protocol), that reveals simultane...

journal_title：Journal of cell science

authors： Chatre L,Ricchetti M

更新日期：2013-02-15 00:00:00

abstract：:The nature, intracellular distribution, and role of proteins synthesized during meiotic maturation of mouse oocytes in vitro have been examined. Proteins synthesized during the initial stages of maturation are concentrated within the nucleus (germinal vesicle) and become intimately associated with the condensing chrom...

journal_title：Journal of cell science

authors： Wassarman PM,Letourneau GE

更新日期：1976-05-01 00:00:00

abstract：:An acrosome reaction was induced in ejaculated ram spermatozoa by treatment with calcium and the ionophore A23187. Samples were fixed at different times after initiation of induction, and the morphological changes within the head membranes that took place as exocytosis occurred were studied in freeze-fracture replicas...

journal_title：Journal of cell science

authors： Fléchon JE,Harrison RA,Fléchon B,Escaig J

更新日期：1986-03-01 00:00:00

abstract：:The study of plasma membrane and phagosome membrane of Dictyostelium discoideum was performed using 2 lectins: concanavalin A (Con A) and Wheat Germ Agglutinin (WGA), and 2 markers of anionic sites: colloidal iron hydroxide (CIH) and cationized ferritin (CF). These labellings were applied to fixed partially broken cel...

journal_title：Journal of cell science

authors： Ryter A,Hellio R

更新日期：1980-02-01 00:00:00

abstract：:The tumor suppressor p53 is activated in response to many forms of cellular stress leading to cell cycle arrest, senescence or apoptosis. Appropriate sub-cellular localization is essential for modulating p53 function. We recently showed that p53 localizes to the nucleolus after proteasome inhibition with MG132 and thi...

journal_title：Journal of cell science

authors： Karni-Schmidt O,Zupnick A,Castillo M,Ahmed A,Matos T,Bouvet P,Cordon-Cardo C,Prives C

更新日期：2008-12-15 00:00:00

abstract：:The centrosome and cilium are organelles with important roles in microtubule organisation, cell division, cell signalling, embryogenesis and tissue homeostasis. The two organelles are mutually exclusive. The centriole/basal body is found at the core of the centrosome (centriole) or at the base of the cilium (basal bod...

journal_title：Journal of cell science

authors： de Saram P,Iqbal A,Murdoch JN,Wilkinson CJ

更新日期：2017-10-01 00:00:00

abstract：:Keratins 8 and 18 (K8 and K18) are regulated by site-specific phosphorylation in response to multiple stresses. We examined the effect and regulation of hyposmotic stress on keratin phosphorylation. K8 phospho-Ser431 (Ser431-P) becomes dephosphorylated in HT29 cells, but hyperphosphorylated on other K8 but not K18 sit...

journal_title：Journal of cell science

authors： Tao GZ,Toivola DM,Zhou Q,Strnad P,Xu B,Michie SA,Omary MB

更新日期：2006-04-01 00:00:00

abstract：:The large GTPase dynamin, long known for its role in endocytosis, has most recently been implicated as a facilitator of cell migration and invasion. Recent observations link dynamin to the cycle of membrane expansion and retraction essential for cell motility. Its role in actin polymerization, membrane deformation and...

journal_title：Journal of cell science

authors： Kruchten AE,McNiven MA

更新日期：2006-05-01 00:00:00

abstract：:Angiogenesis is critical for vascular remodeling during development and contributes to the pathogenesis of diseases such as cancer. Targeted disruption of several EphB class receptor tyrosine kinases results in vascular remodeling defects during embryogenesis. The role of EphA class receptors in vascular remodeling, h...

journal_title：Journal of cell science

authors： Brantley-Sieders DM,Caughron J,Hicks D,Pozzi A,Ruiz JC,Chen J

更新日期：2004-04-15 00:00:00

abstract：:The mechanism responsible for spermatid translocation in the mammalian seminiferous epithelium was proposed to be the microtubule-based transport of specialized junction plaques (ectoplasmic specializations) that occur in Sertoli cell regions attached to spermatid heads. These plaques each consist of a cistern of endo...

journal_title：Journal of cell science

authors： Guttman JA,Kimel GH,Vogl AW

更新日期：2000-06-01 00:00:00

abstract：:Ca(2+) signaling mediated by phospholipase C that produces inositol 1,4,5-trisphosphate [Ins(1,4,5)P(3)] and diacylglycerol (DAG) controls lymphocyte activation. In contrast to store-operated Ca(2+) entry activated by Ins(1,4,5)P(3)-induced Ca(2+) release from endoplasmic reticulum, the importance of DAG-activated Ca(...

journal_title：Journal of cell science

authors： Numaga T,Nishida M,Kiyonaka S,Kato K,Katano M,Mori E,Kurosaki T,Inoue R,Hikida M,Putney JW Jr,Mori Y

更新日期：2010-03-15 00:00:00

abstract：:The Sendai virus-induced fusion of HeLa cells has been studied by freeze-fracture electron microscopy. Freeze-fracture observations confirm previous scanning electron-microscope studies (1977) and show that at 4 degrees C virus particles bind to the cell surface and that cell agglutination results from the crosslinkin...

journal_title：Journal of cell science

authors： Knutton S

更新日期：1979-04-01 00:00:00

abstract：:Asymmetric dimethylarginine (ADMA) is an inhibitor of nitric oxide production associated with abnormal blood vessel growth and repair, however, the mechanism of action of ADMA is not well understood. We studied the role of exogenous and endogenous ADMA in the regulation of cell motility and actin cytoskeleton in porci...

journal_title：Journal of cell science

authors： Wojciak-Stothard B,Torondel B,Tsang LY,Fleming I,Fisslthaler B,Leiper JM,Vallance P

更新日期：2007-03-15 00:00:00

abstract：:Changes in the cytoskeletal structure of cultured A10 smooth muscle cells induced by calyculin-A (CL-A), a potent inhibitor of types 1 and 2A protein phosphatases, were analyzed using indirect fluorescence techniques. In the presence of 1 x 10(-7) M CL-A the cells became round and subsequently detached from the substr...

journal_title：Journal of cell science

authors： Hosoya N,Mitsui M,Yazama F,Ishihara H,Ozaki H,Karaki H,Hartshorne DJ,Mohri H

更新日期：1993-08-01 00:00:00

abstract：:The regulation and function of the crucial cell cycle regulator cyclin E (CycE) remains elusive. Unlike other cyclins, CycE can be uniquely controlled by mitochondrial energetics, the exact mechanism being unclear. Using mammalian cells (in vitro) and Drosophila (in vivo) model systems in parallel, we show that CycE c...

journal_title：Journal of cell science

authors： Parker DJ,Iyer A,Shah S,Moran A,Hjelmeland AB,Basu MK,Liu R,Mitra K

更新日期：2015-11-15 00:00:00

abstract：:Previous studies have shown that unactivated lymphocytes bind to CS1 peptide and that the adhesion of these cells to high endothelium is inhibited by CS1 peptide. These results suggest that lymphocyte binding occurs via recognition of the CS1-containing splice variant of fibronectin expressed on the high endothelial s...

journal_title：Journal of cell science

authors： May MJ,Entwistle G,Humphries MJ,Ager A

更新日期：1993-09-01 00:00:00

abstract：:The alternative lengthening of telomeres (ALT) mechanism allows cancer cells to escape senescence and apoptosis in the absence of active telomerase. A characteristic feature of this pathway is the assembly of ALT-associated promyelocytic leukemia (PML) nuclear bodies (APBs) at telomeres. Here, we dissected the role of...

journal_title：Journal of cell science

authors： Osterwald S,Deeg KI,Chung I,Parisotto D,Wörz S,Rohr K,Erfle H,Rippe K

更新日期：2015-05-15 00:00:00

abstract：:Neutrophil leucocytes circulate in plasma, which contains broad-spectrum antiproteases such as alpha-2-macroglobulin and alpha-1-antitrypsin, and move into environments in which antiproteases are present. Plasma antiproteases significantly reduce the adhesiveness of leucocytes in both static and dynamic adhesion assay...

journal_title：Journal of cell science

authors： Forrester JV,Lackie JM,Brown AF

更新日期：1983-01-01 00:00:00

abstract：:Nuclear actin plays an important role in many processes that regulate gene expression. Cytoplasmic actin dynamics are tightly controlled by numerous actin-binding proteins, but regulation of nuclear actin has remained unclear. Here, we performed a genome-wide RNA interference (RNAi) screen in Drosophila cells to ident...

journal_title：Journal of cell science

authors： Dopie J,Rajakylä EK,Joensuu MS,Huet G,Ferrantelli E,Xie T,Jäälinoja H,Jokitalo E,Vartiainen MK

更新日期：2015-07-01 00:00:00

abstract：:Eukaryogenesis - the emergence of eukaryotic cells - represents a pivotal evolutionary event. With a fundamentally more complex cellular plan compared to prokaryotes, eukaryotes are major contributors to most aspects of life on Earth. For decades, we have understood that eukaryotic origins lie within both the Archaea ...

journal_title：Journal of cell science

authors： Dacks JB,Field MC,Buick R,Eme L,Gribaldo S,Roger AJ,Brochier-Armanet C,Devos DP

更新日期：2016-10-15 00:00:00

abstract：:The beta 2-tubulin isotype of Physarum polycephalum is only 83% identical in amino acid sequence with the constitutively expressed beta 1B-tubulin and the myxamoeba-specific beta 1A-tubulin isotypes. A polyclonal antibody specific for beta 2-tubulin was used to monitor the subcellular distribution of the beta 2-tubuli...

journal_title：Journal of cell science

authors： Diggins-Gilicinski M,Solnica-Krezel L,Burland TG,Paul EC,Dove WF

更新日期：1989-10-01 00:00:00

abstract：:The lymphatic endothelial cell (LEC) fate decision program during development has been described. However, the mechanism underlying the maintenance of differentiated LEC identity remains largely unknown. Here, we show that fibroblast growth factor 2 (FGF2) plays a fundamental role in maintaining a differentiated LEC t...

journal_title：Journal of cell science

authors： Ichise T,Yoshida N,Ichise H

更新日期：2014-02-15 00:00:00

abstract：:alphaA-Crystallin (alphaA) is a molecular chaperone expressed preferentially in the lens. alphaA transcripts are first detected during the early stages of lens development and its synthesis continues as the lens grows throughout life. alphaA(-/-) mouse lenses are smaller than controls, and lens epithelial cells derive...

journal_title：Journal of cell science

authors： Xi JH,Bai F,Andley UP

更新日期：2003-03-15 00:00:00

abstract：:Dispersion of neural crest cells and their ultimate regroupment into peripheral ganglia are associated with precisely coordinated regulations both in time and space of the expression and function of cell adhesion receptors. In particular, the disappearance of N-cadherin from the cell surface at the onset of migration ...

journal_title：Journal of cell science

authors： Monier-Gavelle F,Duband JL

更新日期：1995-12-01 00:00:00