Abstract：
:Nuclear actin plays an important role in many processes that regulate gene expression. Cytoplasmic actin dynamics are tightly controlled by numerous actin-binding proteins, but regulation of nuclear actin has remained unclear. Here, we performed a genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins that influence either nuclear polymerization or import of actin. We validate 19 factors as specific hits, and show that Chinmo (known as Bach2 in mammals), SNF4Aγ (Prkag1 in mammals) and Rab18 play a role in nuclear localization of actin in both fly and mammalian cells. We identify several new regulators of cofilin activity, and characterize modulators of both cofilin kinases and phosphatase. For example, Chinmo/Bach2, which regulates nuclear actin levels also in vivo, maintains active cofilin by repressing the expression of the kinase Cdi (Tesk in mammals). Finally, we show that Nup98 and lamin are candidates for regulating nuclear actin polymerization. Our screen therefore reveals new aspects of actin regulation and links nuclear actin to many cellular processes.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Dopie J,Rajakylä EK,Joensuu MS,Huet G,Ferrantelli E,Xie T,Jäälinoja H,Jokitalo E,Vartiainen MKdoi
10.1242/jcs.169441subject
Has Abstractpub_date
2015-07-01 00:00:00pages
2388-400issue
13eissn
0021-9533issn
1477-9137pii
jcs.169441journal_volume
128pub_type
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更新日期：2002-07-15 00:00:00
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更新日期：1994-11-01 00:00:00
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更新日期：2017-04-15 00:00:00
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