Abstract：
:Phosphoinositides represent a major class of lipids specifically involved in the organization of signaling cascades, maintenance of the identity of organelles and regulation of multiple intracellular trafficking steps. We previously reported that phosphatidylinositol 5-monophosphate (PI5P), produced by the Shigella flexneri phosphatase IpgD, is implicated in the endosomal sorting of the epidermal growth factor receptor (EGFR). Here, we show that the adaptor protein TOM1 is a new direct binding partner of PI5P. We identify the domain of TOM1 involved in this interaction and characterize the binding motif. Finally, we demonstrate that the recruitment of TOM1 by PI5P on signaling endosomes is responsible for the delay in EGFR degradation and fluid-phase bulk endocytosis. Taken together, our data strongly suggest that PI5P enrichment in signaling endosomes prevents endosomal maturation through the recruitment of TOM1, and point to a new function of PI5P in regulating discrete maturation steps in the endosomal system.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Boal F,Mansour R,Gayral M,Saland E,Chicanne G,Xuereb JM,Marcellin M,Burlet-Schiltz O,Sansonetti PJ,Payrastre B,Tronchère Hdoi
10.1242/jcs.166314subject
Has Abstractpub_date
2015-02-15 00:00:00pages
815-27issue
4eissn
0021-9533issn
1477-9137pii
jcs.166314journal_volume
128pub_type
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